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Gene information
General information
TC000688 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7581682..7587439
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000688
    iBeetle screen iB_07280

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000688 RA
      MSQKLKKVC IVGSGNWGS AIAKIVGSN AKKLPHFDD 
      KVTMYVYEE MINGKKLTE IINETHENV KYLPGHKLP 
      PNVVAVPDV VEAAKEADI LIFVVPHQF IRTLCSTLL 
      GKIKPTAIA LSLIKGFDR AEGGGIDLI SHIITRHLR 
      IPCSVLMGA NLAGEVADE NFCETTIGC RDTKQGPLL 
      RDIIQTDYF RVVVVDDED TVEICGALK NIVACGAGF 
      VDGLGLGDN TKAAVIRLG LMEMIKFVD VFYPGGKLS 
      TFFESCGVA DLITTCYGG RNRKVSEAF VKTGKSIKV 
      LEDEMLNGQ KLQGPFTAE EVNYMLKNK GMEEKFPLF 
      TAIHKICTG QKPVAEFID CIRHHPEHM
    • >TC000688 RB
      MSQKLKKVC IVGSGNWGS AIAKIVGSN AKKLPHFDD 
      KVTMYVYEE MINGKKLTE IINETHENV KYLPGHKLP 
      PNVVAVPDV VEAAKEADI LIFVVPHQF IRTLCSTLL 
      GKIKPTAIA LSLIKGFDR AEGGGIDLI SHIITRHLR 
      IPCSVLMGA NLAGEVADE NFCETTIGC RDTKQGPLL 
      RDIIQTDYF RVVVVDDED TVEICGALK NIVACGAGF 
      VDGLGLGDN TKAAVIRLG LMEMIKFVD VFYPGGKLS 
      TFFESCGVA DLITTCYGG RNRKVSEAF VKTGKSIKV 
      LEDEMLNGQ KLQGPFTAE EVNYMLKNK GMEEKFPLF 
      TAIHKICTG QKPVAEFID CIRHHPEHM VKQ
    • >TC000688 RC
      MSQKLKKVC IVGSGNWGS AIAKIVGSN AKKLPHFDD 
      KVTMYVYEE MINGKKLTE IINETHENV KYLPGHKLP 
      PNVVAVPDV VEAAKEADI LIFVVPHQF IRTLCSTLL 
      GKIKPTAIA LSLIKGFDR AEGGGIDLI SHIITRHLR 
      IPCSVLMGA NLAGEVADE NFCETTIGC RDTKQGPLL 
      RDIIQTDYF RVVVVDDED TVEICGALK NIVACGAGF 
      VDGLGLGDN TKAAVIRLG LMEMIKFVD VFYPGGKLS 
      TFFESCGVA DLITTCYGG RNRKVSEAF VKTGKSIKV 
      LEDEMLNGQ KLQGPFTAE EVNYMLKNK GMEEKFPLF 
      TAIHKICTG QKPVAEFID CIRHHPEHM DNSYDRPKC 
      KL
    CDS sequence
    • >TC000688 RA
      atgtctcag aaattaaaa aaagtgtgc atagtaggc 
      agcggcaac tggggctca gccatcgcg aaaattgtc 
      gggagcaat gcgaaaaaa ctaccccat ttcgatgat 
      aaggttacg atgtatgtt tacgaagaa atgataaat 
      gggaaaaaa cttacagag atcattaat gaaacccac 
      gaaaatgtc aaatatctt ccaggtcac aagcttcct 
      cctaatgtc gtggcggtt cccgatgta gtagaggcc 
      gccaaagag gctgatatt cttattttt gtagtgcca 
      caccagttc ataagaact ttatgctca actctttta 
      gggaaaatc aaaccaaca gctattgct ttatctctg 
      attaagggc tttgaccga gcagagggc ggcggaatc 
      gacctcatt tcgcacata atcacccgc cacttgcgc 
      atcccttgc tccgtcctc atgggggcg aacctcgct 
      ggtgaagtg gccgacgag aatttctgc gaaacaacc 
      atcggttgt agagacacc aaacaggga ccactgctc 
      cgcgatatt atccaaact gattatttc cgagtggtc 
      gtagtggac gacgaagac actgtcgag atttgtggg 
      gcgttgaaa aacatcgtc gcgtgtggg gcaggtttc 
      gtggacggt ttgggcttg ggtgacaac accaaggcc 
      gccgtaatc cgattgggg ctcatggag atgatcaaa 
      ttcgtcgat gttttctac ccgggagga aaattgtca 
      accttcttt gaaagttgc ggcgtcgct gatttgatc 
      acgacgtgt tacgggggt agaaatagg aaagtgagc 
      gaagcgttc gtcaaaaca ggaaaatcg ataaaagtg 
      ttggaggac gagatgctg aacggacag aagttgcaa 
      gggcccttc acggcagag gaggtcaac tatatgttg 
      aaaaataag ggcatggag gaaaagttc ccgttgttt 
      acagccatt cataaaata tgtacagga cagaaacca 
      gtcgctgaa tttatcgac tgcattagg caccatcct 
      gagcacatg taa
    • >TC000688 RB
      atgtctcag aaattaaaa aaagtgtgc atagtaggc 
      agcggcaac tggggctca gccatcgcg aaaattgtc 
      gggagcaat gcgaaaaaa ctaccccat ttcgatgat 
      aaggttacg atgtatgtt tacgaagaa atgataaat 
      gggaaaaaa cttacagag atcattaat gaaacccac 
      gaaaatgtc aaatatctt ccaggtcac aagcttcct 
      cctaatgtc gtggcggtt cccgatgta gtagaggcc 
      gccaaagag gctgatatt cttattttt gtagtgcca 
      caccagttc ataagaact ttatgctca actctttta 
      gggaaaatc aaaccaaca gctattgct ttatctctg 
      attaagggc tttgaccga gcagagggc ggcggaatc 
      gacctcatt tcgcacata atcacccgc cacttgcgc 
      atcccttgc tccgtcctc atgggggcg aacctcgct 
      ggtgaagtg gccgacgag aatttctgc gaaacaacc 
      atcggttgt agagacacc aaacaggga ccactgctc 
      cgcgatatt atccaaact gattatttc cgagtggtc 
      gtagtggac gacgaagac actgtcgag atttgtggg 
      gcgttgaaa aacatcgtc gcgtgtggg gcaggtttc 
      gtggacggt ttgggcttg ggtgacaac accaaggcc 
      gccgtaatc cgattgggg ctcatggag atgatcaaa 
      ttcgtcgat gttttctac ccgggagga aaattgtca 
      accttcttt gaaagttgc ggcgtcgct gatttgatc 
      acgacgtgt tacgggggt agaaatagg aaagtgagc 
      gaagcgttc gtcaaaaca ggaaaatcg ataaaagtg 
      ttggaggac gagatgctg aacggacag aagttgcaa 
      gggcccttc acggcagag gaggtcaac tatatgttg 
      aaaaataag ggcatggag gaaaagttc ccgttgttt 
      acagccatt cataaaata tgtacagga cagaaacca 
      gtcgctgaa tttatcgac tgcattagg caccatcct 
      gagcacatg gtcaagcag taa
    • >TC000688 RC
      atgtctcag aaattaaaa aaagtgtgc atagtaggc 
      agcggcaac tggggctca gccatcgcg aaaattgtc 
      gggagcaat gcgaaaaaa ctaccccat ttcgatgat 
      aaggttacg atgtatgtt tacgaagaa atgataaat 
      gggaaaaaa cttacagag atcattaat gaaacccac 
      gaaaatgtc aaatatctt ccaggtcac aagcttcct 
      cctaatgtc gtggcggtt cccgatgta gtagaggcc 
      gccaaagag gctgatatt cttattttt gtagtgcca 
      caccagttc ataagaact ttatgctca actctttta 
      gggaaaatc aaaccaaca gctattgct ttatctctg 
      attaagggc tttgaccga gcagagggc ggcggaatc 
      gacctcatt tcgcacata atcacccgc cacttgcgc 
      atcccttgc tccgtcctc atgggggcg aacctcgct 
      ggtgaagtg gccgacgag aatttctgc gaaacaacc 
      atcggttgt agagacacc aaacaggga ccactgctc 
      cgcgatatt atccaaact gattatttc cgagtggtc 
      gtagtggac gacgaagac actgtcgag atttgtggg 
      gcgttgaaa aacatcgtc gcgtgtggg gcaggtttc 
      gtggacggt ttgggcttg ggtgacaac accaaggcc 
      gccgtaatc cgattgggg ctcatggag atgatcaaa 
      ttcgtcgat gttttctac ccgggagga aaattgtca 
      accttcttt gaaagttgc ggcgtcgct gatttgatc 
      acgacgtgt tacgggggt agaaatagg aaagtgagc 
      gaagcgttc gtcaaaaca ggaaaatcg ataaaagtg 
      ttggaggac gagatgctg aacggacag aagttgcaa 
      gggcccttc acggcagag gaggtcaac tatatgttg 
      aaaaataag ggcatggag gaaaagttc ccgttgttt 
      acagccatt cataaaata tgtacagga cagaaacca 
      gtcgctgaa tttatcgac tgcattagg caccatcct 
      gagcacatg gacaatagt tatgacagg ccaaagtgt 
      aaactataa
    mRNA sequence
    • >TC000688 RA
      aatatttat attttgata aaaacattt ttattgcca 
      aggcacttg tgatgtaat agccccaat gctgccatc 
      gtgccatgc aatttccca tgcgattac ataacgcaa 
      ctcgtgata taaggcaac tcctctgga gggtgaatt 
      gcaattttc gaccttaaa gttcgcggg tccaccttt 
      catctagcc aaggttcgc gtttgaata aatttgatc 
      ggagtcaag tagatgacc tcccggcag ccctatgat 
      tggcttctt gtcgtttta tcaatctct gattggcag 
      gattagaat cgacgtcgg tcgtcgttt ttgggcggt 
      atcaacctt atcagctag gtttaaacc acaacaatc 
      gcggcagtc gttgacaag taagcgttc cagctgtca 
      ctcaactaa cctctttga atcgtccgc gagaatgtc 
      tcagaaatt aaaaaaagt gtgcatagt aggcagcgg 
      caactgggg ctcagccat cgcgaaaat tgtcgggag 
      caatgcgaa aaaactacc ccatttcga tgataaggt 
      tacgatgta tgtttacga agaaatgat aaatgggaa 
      aaaacttac agagatcat taatgaaac ccacgaaaa 
      tgtcaaata tcttccagg tcacaagct tcctcctaa 
      tgtcgtggc ggttcccga tgtagtaga ggccgccaa 
      agaggctga tattcttat ttttgtagt gccacacca 
      gttcataag aactttatg ctcaactct tttagggaa 
      aatcaaacc aacagctat tgctttatc tctgattaa 
      gggctttga ccgagcaga gggcggcgg aatcgacct 
      catttcgca cataatcac ccgccactt gcgcatccc 
      ttgctccgt cctcatggg ggcgaacct cgctggtga 
      agtggccga cgagaattt ctgcgaaac aaccatcgg 
      ttgtagaga caccaaaca gggaccact gctccgcga 
      tattatcca aactgatta tttccgagt ggtcgtagt 
      ggacgacga agacactgt cgagatttg tggggcgtt 
      gaaaaacat cgtcgcgtg tggggcagg tttcgtgga 
      cggtttggg cttgggtga caacaccaa ggccgccgt 
      aatccgatt ggggctcat ggagatgat caaattcgt 
      cgatgtttt ctacccggg aggaaaatt gtcaacctt 
      ctttgaaag ttgcggcgt cgctgattt gatcacgac 
      gtgttacgg gggtagaaa taggaaagt gagcgaagc 
      gttcgtcaa aacaggaaa atcgataaa agtgttgga 
      ggacgagat gctgaacgg acagaagtt gcaagggcc 
      cttcacggc agaggaggt caactatat gttgaaaaa 
      taagggcat ggaggaaaa gttcccgtt gtttacagc 
      cattcataa aatatgtac aggacagaa accagtcgc 
      tgaatttat cgactgcat taggcacca tcctgagca 
      catgtaagt gcttttcac aaaataaca cagagtaca 
      acttgctaa tgcatgtgc tataaacat tatttaccg 
      caaagcaaa gtccgttga tgagaccgt ctagtaatt 
      attacatta attaccaat tatttgtta tgttttttt 
      actaggtgg tcgtttata tatttttat actatacaa 
      ttgttgcgt agtttttag ctacattgt tatgttttt 
      atctttact aatatgttt gagattgcg aaaattcta 
      ttgattgtt atgcatgta attaagttg ctatagaga 
      acaggaatt ttacagggt ttttacaat atattttat 
      ttattttaa tcttctaac gtgactcaa tctagagtt 
      taatgtatt gatcatgta gattagtaa gtatggctt 
      cttgctttt gaaattaac cggaacaga ttaattccc 
      cgttacata tcagaattt tcgtgattt tttttcaag 
      caatttgtc atgtcttgc tattttaat ttttttacc 
      tcatgtata aatatcatt tgctatgcc atgcatgta 
      tttgttata acttgtaac aatgccaat agaaaaggt 
      gttaatttg aagtagcga gtgccttga aaatctttg 
      gaaaacatc atataatac atttcatca aaagtatac 
      taataacaa tcgtgtgta ggtgataat tagtcattc 
      cgccaaatt tacttaaat atgtactaa aaccacttc 
      aacttcagt caataccta aatcattta tttcacgag 
      tgtagatgt atacttgta atttttgat aaaaacttc 
      aggtgtgtg tgtgtgata actgataac attgtgtgt 
      gagatttgg accatacgt aattataat attttcgct 
      aaaagtaca agatgtccg gatttgtga ataatcggt 
      gaataaaaa tattagcaa cca
    • >TC000688 RB
      aatatttat attttgata aaaacattt ttattgcca 
      aggcacttg tgatgtaat agccccaat gctgccatc 
      gtgccatgc aatttccca tgcgattac ataacgcaa 
      ctcgtgata taaggcaac tcctctgga gggtgaatt 
      gcaattttc gaccttaaa gttcgcggg tccaccttt 
      catctagcc aaggttcgc gtttgaata aatttgatc 
      ggagtcaag tagatgacc tcccggcag ccctatgat 
      tggcttctt gtcgtttta tcaatctct gattggcag 
      gattagaat cgacgtcgg tcgtcgttt ttgggcggt 
      atcaacctt atcagctag gtttaaacc acaacaatc 
      gcggcagtc gttgacaag taagcgttc cagctgtca 
      ctcaactaa cctctttga atcgtccgc gagaatgtc 
      tcagaaatt aaaaaaagt gtgcatagt aggcagcgg 
      caactgggg ctcagccat cgcgaaaat tgtcgggag 
      caatgcgaa aaaactacc ccatttcga tgataaggt 
      tacgatgta tgtttacga agaaatgat aaatgggaa 
      aaaacttac agagatcat taatgaaac ccacgaaaa 
      tgtcaaata tcttccagg tcacaagct tcctcctaa 
      tgtcgtggc ggttcccga tgtagtaga ggccgccaa 
      agaggctga tattcttat ttttgtagt gccacacca 
      gttcataag aactttatg ctcaactct tttagggaa 
      aatcaaacc aacagctat tgctttatc tctgattaa 
      gggctttga ccgagcaga gggcggcgg aatcgacct 
      catttcgca cataatcac ccgccactt gcgcatccc 
      ttgctccgt cctcatggg ggcgaacct cgctggtga 
      agtggccga cgagaattt ctgcgaaac aaccatcgg 
      ttgtagaga caccaaaca gggaccact gctccgcga 
      tattatcca aactgatta tttccgagt ggtcgtagt 
      ggacgacga agacactgt cgagatttg tggggcgtt 
      gaaaaacat cgtcgcgtg tggggcagg tttcgtgga 
      cggtttggg cttgggtga caacaccaa ggccgccgt 
      aatccgatt ggggctcat ggagatgat caaattcgt 
      cgatgtttt ctacccggg aggaaaatt gtcaacctt 
      ctttgaaag ttgcggcgt cgctgattt gatcacgac 
      gtgttacgg gggtagaaa taggaaagt gagcgaagc 
      gttcgtcaa aacaggaaa atcgataaa agtgttgga 
      ggacgagat gctgaacgg acagaagtt gcaagggcc 
      cttcacggc agaggaggt caactatat gttgaaaaa 
      taagggcat ggaggaaaa gttcccgtt gtttacagc 
      cattcataa aatatgtac aggacagaa accagtcgc 
      tgaatttat cgactgcat taggcacca tcctgagca 
      catggtcaa gcagtaaca gtgaataaa tcaaacagc 
      taattattt tcttccaat aggtgtcaa tattataga 
      aaaaatagt cacaaataa gaagtacaa aaaatgtgt 
      tctgcaaat attgtaact taggcaaag ctagttttg 
      gatagtatt ttttacact caaaatcca agggtgtat 
      taagtatta tttgttgaa ccataaatg tacttgaac 
      gattttgtt ctttgattc ccccctcaa aaaaccaac 
      tccttgcaa catattaac ttatat
    • >TC000688 RC
      aatatttat attttgata aaaacattt ttattgcca 
      aggcacttg tgatgtaat agccccaat gctgccatc 
      gtgccatgc aatttccca tgcgattac ataacgcaa 
      ctcgtgata taaggcaac tcctctgga gggtgaatt 
      gcaattttc gaccttaaa gttcgcggg tccaccttt 
      catctagcc aaggttcgc gtttgaata aatttgatc 
      ggagtcaag tagatgacc tcccggcag ccctatgat 
      tggcttctt gtcgtttta tcaatctct gattggcag 
      gattagaat cgacgtcgg tcgtcgttt ttgggcggt 
      atcaacctt atcagctag gtttaaacc acaacaatc 
      gcggcagtc gttgacaag taagcgttc cagctgtca 
      ctcaactaa cctctttga atcgtccgc gagaatgtc 
      tcagaaatt aaaaaaagt gtgcatagt aggcagcgg 
      caactgggg ctcagccat cgcgaaaat tgtcgggag 
      caatgcgaa aaaactacc ccatttcga tgataaggt 
      tacgatgta tgtttacga agaaatgat aaatgggaa 
      aaaacttac agagatcat taatgaaac ccacgaaaa 
      tgtcaaata tcttccagg tcacaagct tcctcctaa 
      tgtcgtggc ggttcccga tgtagtaga ggccgccaa 
      agaggctga tattcttat ttttgtagt gccacacca 
      gttcataag aactttatg ctcaactct tttagggaa 
      aatcaaacc aacagctat tgctttatc tctgattaa 
      gggctttga ccgagcaga gggcggcgg aatcgacct 
      catttcgca cataatcac ccgccactt gcgcatccc 
      ttgctccgt cctcatggg ggcgaacct cgctggtga 
      agtggccga cgagaattt ctgcgaaac aaccatcgg 
      ttgtagaga caccaaaca gggaccact gctccgcga 
      tattatcca aactgatta tttccgagt ggtcgtagt 
      ggacgacga agacactgt cgagatttg tggggcgtt 
      gaaaaacat cgtcgcgtg tggggcagg tttcgtgga 
      cggtttggg cttgggtga caacaccaa ggccgccgt 
      aatccgatt ggggctcat ggagatgat caaattcgt 
      cgatgtttt ctacccggg aggaaaatt gtcaacctt 
      ctttgaaag ttgcggcgt cgctgattt gatcacgac 
      gtgttacgg gggtagaaa taggaaagt gagcgaagc 
      gttcgtcaa aacaggaaa atcgataaa agtgttgga 
      ggacgagat gctgaacgg acagaagtt gcaagggcc 
      cttcacggc agaggaggt caactatat gttgaaaaa 
      taagggcat ggaggaaaa gttcccgtt gtttacagc 
      cattcataa aatatgtac aggacagaa accagtcgc 
      tgaatttat cgactgcat taggcacca tcctgagca 
      catggacaa tagttatga caggccaaa gtgtaaact 
      ataacttag tgttgtatt tcctttgat ttaaacgtt 
      ataagcaac taacgaaat caaagcttc aagttaaca 
      attcatgtg gttgtcgta aaattggtg ttgaataag 
      ttaagactg atttttttg ctagttgaa taaatagca 
      tttaaatgg ggcgtttta ttaaaacat catccgttt 
      cattgtact aacacctca ataattcaa ttaggaaag 
      taattatta cataattgt atttttcca gggtcaagc 
      agtaacagt gaataaatc aaacagcta attattttc 
      ttccaatag gtgtcaata ttatagaaa aaatagtca 
      caaataaga agtacaaaa aatgtgttc tgcaaatat 
      tgtaactta ggcaaagct agttttgga tagtatttt 
      ttacactca aaatccaag ggtgtatta agtattatt 
      tgttgaacc ataaatgta cttgaacga ttttgttct 
      ttgattccc ccctcaaaa aaccaactc cttgcaaca 
      tattaactt atat
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