Status
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Gene information
General information
TC034075
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005708,TC005709,TC005707,TC005706
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8585036..8619698
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034075
    iBeetle screen iB_07277

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034075 RA
      MCVIFFKEK CRITQIQED EDEASENPD VQSADLLGS 
      APPSSNQWG SRCGFGPFL AKVRLQCWR AADMITSPL 
      LGLCWLPNN RVFSVQSAH NKILGRLVW NQHQCFLTI 
      GQAQSVTPY VGFWNSVAI FQPSSHPFN METRYKSGI 
      RSSAVRSSV VASGGGGGG GGGGSGRLC EVRRAYKAA 
      VVPCKTGRR RARGMSMGQ KVSGVKAVN RDAAAPYKP 
      VIPRELAQD FARPPRLDM LLDMPAALR ETQLKHSWN 
      QDDRSLNIF VKEDDKLTF HRHPVAQST DCIRGKVGF 
      TKGMHCWEV CWSTRQRGT HAVVGVATA EAPLHSVGY 
      QSLVGSTDT SWGWDLGRN KLYHDSKNC PGVTYPTLL 
      KPDETFIVP DKFLVVLDM DEGTLSFVV DGQYLGVAF 
      RGLKGRKLY PIVSAVWGH CEITMRYIG GLDPEPLPL 
      MDLCRRVIR QRLGKQHLE EKVMNLQLP QALQTYLLY 
      RDRR
    • >TC034075 RB
      MCVIFFKEK CRITQIQED EDEASENPD VQSADLLGS 
      APPSSNQWG SRCGFGPFL AKVRLQCWR AADMITSPL 
      LGLCWLPNN RVFSVQSAH NKILGRLVW NQHQCFLTI 
      GQAQSVTPY VGFWNSVAI FQPSSHPFN METRSENSA 
      PHLPLPPES YLHIFPPNT STVPLSLQA KAILEVAQF 
      HNTHPIVKK FRLRSKMSG NRKTSPRSF IFGRFVILI 
      GGIPTPLAR GPLVTPKLF VVESISILG ANSGRQFRC 
      RATIEGRFR GSDPPDSGG SVLRRVRVA PGLRWLSEM 
      DFNNAGFFV WWPFNARFL MDVANQIRQ SFVVESTVP 
      FRFLSLRHT SLLPLSLIS SSLFGAFTR VVLQLPLRH 
      ANRRCNVTS VGVLVSFFQ QGRKKMEYA RRKYKSGIR 
      SSAVRSSVV ASGGGGGGG GGGSGRLCE VRRAYKAAV 
      VPCKTGRRR ARGMSMGQK VSGVKAVNR DAAAPYKPV 
      IPRELAQDF ARPPRLDML LDMPAALRE TQLKHSWNQ 
      DDRSLNIFV KEDDKLTFH RHPVAQSTD CIRGKVGFT 
      KGMHCWEVC WSTRQRGTH AVVGVATAE APLHSVGYQ 
      SLVGSTDTS WGWDLGRNK LYHDSKNCP GVTYPTLLK 
      PDETFIVPD KFLVVLDMD EGTLSFVVD GQYLGVAFR 
      GLKGRKLYP IVSAVWGHC EITMRYIGG LDPEPLPLM 
      DLCRRVIRQ RLGKQHLEE KVMNLQLPQ ALQTYLLYR 
      DRR
    • >TC034075 RC
      MKFMEAHKY KFVGYGVRP ALRIRTLAL RNPAFYWLR 
      GSAPPSSNQ WGSRCGFGP FLAKVRLQC WRAADMITS 
      PLLGLCWLP NNRVFSVQS AHNKILGRL VWNQHQCFL 
      TIGQAQSVT PYVGFWNSV AIFQPSSHP FNMETRSEN 
      SAPHLPLPP ESYLHIFPP NTSTVPLSL QAKAILEVA 
      QFHNTHPIV KKFRLRSKM SGNRKTSPR SFIFGRFVI 
      LIGGIPTPL ARGPLVTPK LFVVESISI LGANSGRQF 
      RCRATIEGR FRGSDPPDS GGSVLRRVR VAPGLRWLS 
      EMDFNNAGF FVWWPFNAR FLMDVANQI RQSFVVEST 
      VPFRFLSLR HTSLLPLSL ISSSLFGAF TRVVLQLPL 
      RHANRRCNV TSVGVLVSF FQQGRKKME YARRKYKSG 
      IRSSAVRSS VVASGGGGG GGGGGSGRL CEVRRAYKA 
      AVVPCKTGR RRARGMSMG QKVSGVKAV NRDAAAPYK 
      PVIPRELAQ DFARPPRLD MLLDMPAAL RETQLKHSW 
      NQDDRSLNI FVKEDDKLT FHRHPVAQS TDCIRGKVG 
      FTKGMHCWE VCWSTRQRG THAVVGVAT AEAPLHSVG 
      YQSLVGSTD TSWGWDLGR NKLYHDSKN CPGVTYPTL 
      LKPDETFIV PDKFLVVLD MDEGTLSFV VDGQYLGVA 
      FRGLKGRKL YPIVSAVWG HCEITMRYI GGLDPEPLP 
      LMDLCRRVI RQRLGKQHL EEKVMNLQL PQALQTYLL 
      YRDRR
    CDS sequence
    • >TC034075 RA
      atgtgtgtg atttttttc aaagaaaag tgcaggata 
      acacaaatt caggaggac gaggatgag gcatccgaa 
      aatcctgac gtacaatcc gcggattta ttagggtcc 
      gcgcctccc tcttcgaac caatggggc tcccggtgc 
      ggcttcgga ccgtttctt gccaaggtt agacttcag 
      tgttggcga gcagcggac atgataact tctcccctg 
      ctcggactc tgttggctg cctaataac cgtgtgttt 
      agtgtacaa tccgctcac aataagata ctcggacgt 
      ttagtgtgg aaccaacat cagtgtttt ttgacaata 
      ggacaagcg caatctgtg actccgtat gtcggattt 
      tggaatagt gtcgctatt tttcaacca tcatcacac 
      ccctttaat atggaaaca aggtacaaa agcggcatc 
      cgcagcagc gcagtgagg agcagcgtg gtagccagc 
      ggaggcggc ggcggcggc ggcggcggg ggcagcggt 
      aggctgtgc gaagtgagg cgcgcatac aaagcggcc 
      gtggtgccg tgcaaaacg ggccgtcgc agggcccgg 
      ggcatgagc atgggccag aaggtgtca ggagtcaag 
      gctgttaac cgggatgca gccgcaccc tacaagccc 
      gtcatccca agggaactg gcgcaggat ttcgctcgt 
      ccaccgcgg ctggacatg ctgctcgac atgcctgca 
      gctctcagg gagacccaa ctgaagcac agttggaac 
      caggacgac cgctctctc aacatcttc gtcaaagag 
      gacgacaaa ctgaccttc catagacat cccgtggcg 
      caaagcacg gactgtata cgaggcaaa gtcggcttc 
      acgaagggc atgcactgt tgggaggtg tgttggtcg 
      acgaggcag cgaggcacg catgcagtg gtgggcgtc 
      gcgacggcc gaggcgccg ctgcattcg gtcggatac 
      cagagccta gtcggcagc acggacacg tcgtggggg 
      tgggattta ggtcgaaat aaactctac cacgattcg 
      aaaaactgt cccggtgtg acgtacccg actttatta 
      aagccggac gagactttc atagtgccc gataagttt 
      ttagtggtg ttagatatg gatgagggg acacttagt 
      tttgttgtg gacggccag tacttggga gtagcgttc 
      aggggactg aaagggagg aagttgtat ccaatcgtt 
      agtgccgtt tggggtcac tgtgagatc accatgagg 
      tacattgga ggacttgat ccggagcct ttgccgctg 
      atggacctc tgccggcgc gtgatccgg cagcgctta 
      ggcaagcag cacctcgag gagaaagtg atgaacctg 
      cagctgccc caagctcta cagacttac ctcttgtac 
      agagatcgc agataa
    • >TC034075 RB
      atgtgtgtg atttttttc aaagaaaag tgcaggata 
      acacaaatt caggaggac gaggatgag gcatccgaa 
      aatcctgac gtacaatcc gcggattta ttagggtcc 
      gcgcctccc tcttcgaac caatggggc tcccggtgc 
      ggcttcgga ccgtttctt gccaaggtt agacttcag 
      tgttggcga gcagcggac atgataact tctcccctg 
      ctcggactc tgttggctg cctaataac cgtgtgttt 
      agtgtacaa tccgctcac aataagata ctcggacgt 
      ttagtgtgg aaccaacat cagtgtttt ttgacaata 
      ggacaagcg caatctgtg actccgtat gtcggattt 
      tggaatagt gtcgctatt tttcaacca tcatcacac 
      ccctttaat atggaaaca agaagcgaa aatagcgcg 
      ccccatttg cctttaccc cctgagagc tacctgcac 
      atttttcca ccaaacacc tcgaccgta ccactttct 
      ttacaggcc aaggcgatt cttgaggtc gcacagttt 
      cacaacacc catccaatt gtgaaaaag ttccgttta 
      cgatcgaaa atgagcggc aatcgcaaa acttcccct 
      cgctctttt attttcggt cggtttgtt atccttatc 
      ggtggtatt ccgacccct ctggctcgg ggacccttg 
      gtaacaccc aaacttttc gttgttgaa agcattagt 
      attctcgga gcgaattcg ggaaggcag tttcgatgt 
      cgtgcgaca atagagggt aggtttcgc ggcagcgac 
      cctccggac tccggaggt tcagtcttg agacgggtg 
      cgtgtggct ccgggtctt cgatggcta tcggagatg 
      gattttaat aacgcgggt tttttcgtt tggtggccg 
      tttaatgcg agatttttg atggatgtg gccaatcaa 
      attcggcag tcgttcgtc gtcgaatcg acggttccg 
      tttcgtttt ctctcactc cgacacacg tctcttctc 
      cctctctct ctcatttcg agttctttg ttcggcgcg 
      ttcacacgt gttgtgctt cagttaccg ctcaggcat 
      gccaaccgt cggtgtaac gtcacgtcc gttggtgtt 
      ttagtgtcc ttttttcaa caggggagg aaaaaaatg 
      gaatacgcc aggcggaag tacaaaagc ggcatccgc 
      agcagcgca gtgaggagc agcgtggta gccagcgga 
      ggcggcggc ggcggcggc ggcgggggc agcggtagg 
      ctgtgcgaa gtgaggcgc gcatacaaa gcggccgtg 
      gtgccgtgc aaaacgggc cgtcgcagg gcccggggc 
      atgagcatg ggccagaag gtgtcagga gtcaaggct 
      gttaaccgg gatgcagcc gcaccctac aagcccgtc 
      atcccaagg gaactggcg caggatttc gctcgtcca 
      ccgcggctg gacatgctg ctcgacatg cctgcagct 
      ctcagggag acccaactg aagcacagt tggaaccag 
      gacgaccgc tctctcaac atcttcgtc aaagaggac 
      gacaaactg accttccat agacatccc gtggcgcaa 
      agcacggac tgtatacga ggcaaagtc ggcttcacg 
      aagggcatg cactgttgg gaggtgtgt tggtcgacg 
      aggcagcga ggcacgcat gcagtggtg ggcgtcgcg 
      acggccgag gcgccgctg cattcggtc ggataccag 
      agcctagtc ggcagcacg gacacgtcg tgggggtgg 
      gatttaggt cgaaataaa ctctaccac gattcgaaa 
      aactgtccc ggtgtgacg tacccgact ttattaaag 
      ccggacgag actttcata gtgcccgat aagttttta 
      gtggtgtta gatatggat gaggggaca cttagtttt 
      gttgtggac ggccagtac ttgggagta gcgttcagg 
      ggactgaaa gggaggaag ttgtatcca atcgttagt 
      gccgtttgg ggtcactgt gagatcacc atgaggtac 
      attggagga cttgatccg gagcctttg ccgctgatg 
      gacctctgc cggcgcgtg atccggcag cgcttaggc 
      aagcagcac ctcgaggag aaagtgatg aacctgcag 
      ctgccccaa gctctacag acttacctc ttgtacaga 
      gatcgcaga taa
    • >TC034075 RC
      atgaaattt atggaggca cacaaatat aagtttgtc 
      ggatatggg gtgcgcccg gcgctccga attcgaacc 
      ttggcgctc cgaaatccg gcgttctat tggctcaga 
      gggtccgcg cctccctct tcgaaccaa tggggctcc 
      cggtgcggc ttcggaccg tttcttgcc aaggttaga 
      cttcagtgt tggcgagca gcggacatg ataacttct 
      cccctgctc ggactctgt tggctgcct aataaccgt 
      gtgtttagt gtacaatcc gctcacaat aagatactc 
      ggacgttta gtgtggaac caacatcag tgttttttg 
      acaatagga caagcgcaa tctgtgact ccgtatgtc 
      ggattttgg aatagtgtc gctattttt caaccatca 
      tcacacccc tttaatatg gaaacaaga agcgaaaat 
      agcgcgccc catttgcct ttaccccct gagagctac 
      ctgcacatt tttccacca aacacctcg accgtacca 
      ctttcttta caggccaag gcgattctt gaggtcgca 
      cagtttcac aacacccat ccaattgtg aaaaagttc 
      cgtttacga tcgaaaatg agcggcaat cgcaaaact 
      tcccctcgc tcttttatt ttcggtcgg tttgttatc 
      cttatcggt ggtattccg acccctctg gctcgggga 
      cccttggta acacccaaa cttttcgtt gttgaaagc 
      attagtatt ctcggagcg aattcggga aggcagttt 
      cgatgtcgt gcgacaata gagggtagg tttcgcggc 
      agcgaccct ccggactcc ggaggttca gtcttgaga 
      cgggtgcgt gtggctccg ggtcttcga tggctatcg 
      gagatggat tttaataac gcgggtttt ttcgtttgg 
      tggccgttt aatgcgaga tttttgatg gatgtggcc 
      aatcaaatt cggcagtcg ttcgtcgtc gaatcgacg 
      gttccgttt cgttttctc tcactccga cacacgtct 
      cttctccct ctctctctc atttcgagt tctttgttc 
      ggcgcgttc acacgtgtt gtgcttcag ttaccgctc 
      aggcatgcc aaccgtcgg tgtaacgtc acgtccgtt 
      ggtgtttta gtgtccttt tttcaacag gggaggaaa 
      aaaatggaa tacgccagg cggaagtac aaaagcggc 
      atccgcagc agcgcagtg aggagcagc gtggtagcc 
      agcggaggc ggcggcggc ggcggcggc gggggcagc 
      ggtaggctg tgcgaagtg aggcgcgca tacaaagcg 
      gccgtggtg ccgtgcaaa acgggccgt cgcagggcc 
      cggggcatg agcatgggc cagaaggtg tcaggagtc 
      aaggctgtt aaccgggat gcagccgca ccctacaag 
      cccgtcatc ccaagggaa ctggcgcag gatttcgct 
      cgtccaccg cggctggac atgctgctc gacatgcct 
      gcagctctc agggagacc caactgaag cacagttgg 
      aaccaggac gaccgctct ctcaacatc ttcgtcaaa 
      gaggacgac aaactgacc ttccataga catcccgtg 
      gcgcaaagc acggactgt atacgaggc aaagtcggc 
      ttcacgaag ggcatgcac tgttgggag gtgtgttgg 
      tcgacgagg cagcgaggc acgcatgca gtggtgggc 
      gtcgcgacg gccgaggcg ccgctgcat tcggtcgga 
      taccagagc ctagtcggc agcacggac acgtcgtgg 
      gggtgggat ttaggtcga aataaactc taccacgat 
      tcgaaaaac tgtcccggt gtgacgtac ccgacttta 
      ttaaagccg gacgagact ttcatagtg cccgataag 
      tttttagtg gtgttagat atggatgag gggacactt 
      agttttgtt gtggacggc cagtacttg ggagtagcg 
      ttcagggga ctgaaaggg aggaagttg tatccaatc 
      gttagtgcc gtttggggt cactgtgag atcaccatg 
      aggtacatt ggaggactt gatccggag cctttgccg 
      ctgatggac ctctgccgg cgcgtgatc cggcagcgc 
      ttaggcaag cagcacctc gaggagaaa gtgatgaac 
      ctgcagctg ccccaagct ctacagact tacctcttg 
      tacagagat cgcagataa
    mRNA sequence
    • >TC034075 RA
      acaattggc catcagtaa cagcgcaca gttatgttt 
      atgtagata taagacggt cttaaagac tgtgttatt 
      tctttattt tattgttaa ttgacgcaa agttaatta 
      acgcgcctg ttgtgatag ccagttttt gggccaagt 
      gcaccagtg ctccatttt ttgcgtgtt ttgttaccc 
      aacgttaca aatcgtact aaccccaaa acaaactcc 
      ggcaccacc ggaggaacg tccgtccgt cgtctgcgc 
      actctacga gcatacgtc gctgtcgtt gtgtgtttt 
      tgaaaaatg ttacggtgc gctcctggt gtgtggtcc 
      ggtgtatag aaggatttt ttcttcggc ttgaacgct 
      tttaaaaag tgcgaggtg aacgttgcg atgccgatg 
      ttgaaactg tgccgtcga agtcgttgc atccgaagt 
      ttgtttatt tttagacgg ggccaaatg tgcgtgggt 
      aattttcat tctgtctag gttctgaat gtgtgtgat 
      ttttttcaa agaaaagtg caggataac acaaattca 
      ggaggacga ggatgaggc atccgaaaa tcctgacgt 
      acaatccgc ggatttatt agggtccgc gcctccctc 
      ttcgaacca atggggctc ccggtgcgg cttcggacc 
      gtttcttgc caaggttag acttcagtg ttggcgagc 
      agcggacat gataacttc tcccctgct cggactctg 
      ttggctgcc taataaccg tgtgtttag tgtacaatc 
      cgctcacaa taagatact cggacgttt agtgtggaa 
      ccaacatca gtgtttttt gacaatagg acaagcgca 
      atctgtgac tccgtatgt cggattttg gaatagtgt 
      cgctatttt tcaaccatc atcacaccc ctttaatat 
      ggaaacaag gtacaaaag cggcatccg cagcagcgc 
      agtgaggag cagcgtggt agccagcgg aggcggcgg 
      cggcggcgg cggcggggg cagcggtag gctgtgcga 
      agtgaggcg cgcatacaa agcggccgt ggtgccgtg 
      caaaacggg ccgtcgcag ggcccgggg catgagcat 
      gggccagaa ggtgtcagg agtcaaggc tgttaaccg 
      ggatgcagc cgcacccta caagcccgt catcccaag 
      ggaactggc gcaggattt cgctcgtcc accgcggct 
      ggacatgct gctcgacat gcctgcagc tctcaggga 
      gacccaact gaagcacag ttggaacca ggacgaccg 
      ctctctcaa catcttcgt caaagagga cgacaaact 
      gaccttcca tagacatcc cgtggcgca aagcacgga 
      ctgtatacg aggcaaagt cggcttcac gaagggcat 
      gcactgttg ggaggtgtg ttggtcgac gaggcagcg 
      aggcacgca tgcagtggt gggcgtcgc gacggccga 
      ggcgccgct gcattcggt cggatacca gagcctagt 
      cggcagcac ggacacgtc gtgggggtg ggatttagg 
      tcgaaataa actctacca cgattcgaa aaactgtcc 
      cggtgtgac gtacccgac tttattaaa gccggacga 
      gactttcat agtgcccga taagttttt agtggtgtt 
      agatatgga tgaggggac acttagttt tgttgtgga 
      cggccagta cttgggagt agcgttcag gggactgaa 
      agggaggaa gttgtatcc aatcgttag tgccgtttg 
      gggtcactg tgagatcac catgaggta cattggagg 
      acttgatcc ggagccttt gccgctgat ggacctctg 
      ccggcgcgt gatccggca gcgcttagg caagcagca 
      cctcgagga gaaagtgat gaacctgca gctgcccca 
      agctctaca gacttacct cttgtacag agatcgcag 
      ataataaca gtgcttctt agtgcagtg aagtgtcgg 
      acaaaattt tattatcat ccgatcgtg tgtcagctg 
      tcaggtgta gagctaaac caaccaaaa agaatctaa 
      aaattgacg gaggaatgt gtctcagag cgagcgaga 
      gggagagaa agtacgacg cggacgtat ttcgtttcg 
      gacgatttt tattatgat ttcgttaat tggacacac 
      tcaagtctt cgcgctagt tctatattt gtgctgtga 
      atggtacga ttgatttct ttcctcatg atttcgatt 
      gaaacaaac aaataacgg aaacttcct gatctcggg 
      acaagccaa cttgagaga acaatgcca taaggtgta 
      catagttgt aactcagtc ttcttaact tatattctc 
      ggcacggct tcctgatat agctttaca aaatagatt 
      tttaaactt ttttgggtc aagtgtact cgattcgag 
      gatggttgt atacaggga gcgtcagcg aatacaacg 
      aatcgaggg cgtgccttc ggtggcgcc ctctgtgtt 
      gacttttgt acattcgtc ggagctgaa tggaattac 
      accaagata atattactg tatgtaaga atattagat 
      tatttttgt aaatattta gttagtgcc agtctcatt 
      acaccataa tttatgtct gatgcgaac aatcgactg 
      ctctgttct atgagatta atgataatt tttaagagt 
      gtataagat tttttcttt ttttgcatt attgctgaa 
      tccgggcca tctaatggc ctgtcgtga ggtttagaa 
      tgtcggagt gtatgtaat tgtgtattg tgttagttt 
      ttagattgt ttattaaga aatttcaaa acattacgt 
      gttcattag ataaatttt tgcaattat tgtttttac 
      tgttttttt cttttttcg ttttatggt tttaaacaa 
      gcaaaagtt ctcgaatcg ttttggtga tagatttcg 
      accagattt ttttggtct tgttagaat attgaaaat 
      gcaatgcgt agtggcctg gactactat gatattgat 
      aagtagtat gttttattg tattctaag tttcgctgt 
      gaatgtcca ggcctatat ttcagttac tcgatgtat 
      gattatact aaataataa taagtaaac tcttta
    • >TC034075 RB
      acaattggc catcagtaa cagcgcaca gttatgttt 
      atgtagata taagacggt cttaaagac tgtgttatt 
      tctttattt tattgttaa ttgacgcaa agttaatta 
      acgcgcctg ttgtgatag ccagttttt gggccaagt 
      gcaccagtg ctccatttt ttgcgtgtt ttgttaccc 
      aacgttaca aatcgtact aaccccaaa acaaactcc 
      ggcaccacc ggaggaacg tccgtccgt cgtctgcgc 
      actctacga gcatacgtc gctgtcgtt gtgtgtttt 
      tgaaaaatg ttacggtgc gctcctggt gtgtggtcc 
      ggtgtatag aaggatttt ttcttcggc ttgaacgct 
      tttaaaaag tgcgaggtg aacgttgcg atgccgatg 
      ttgaaactg tgccgtcga agtcgttgc atccgaagt 
      ttgtttatt tttagacgg ggccaaatg tgcgtgggt 
      aattttcat tctgtctag gttctgaat gtgtgtgat 
      ttttttcaa agaaaagtg caggataac acaaattca 
      ggaggacga ggatgaggc atccgaaaa tcctgacgt 
      acaatccgc ggatttatt agggtccgc gcctccctc 
      ttcgaacca atggggctc ccggtgcgg cttcggacc 
      gtttcttgc caaggttag acttcagtg ttggcgagc 
      agcggacat gataacttc tcccctgct cggactctg 
      ttggctgcc taataaccg tgtgtttag tgtacaatc 
      cgctcacaa taagatact cggacgttt agtgtggaa 
      ccaacatca gtgtttttt gacaatagg acaagcgca 
      atctgtgac tccgtatgt cggattttg gaatagtgt 
      cgctatttt tcaaccatc atcacaccc ctttaatat 
      ggaaacaag aagcgaaaa tagcgcgcc ccatttgcc 
      tttaccccc tgagagcta cctgcacat ttttccacc 
      aaacacctc gaccgtacc actttcttt acaggccaa 
      ggcgattct tgaggtcgc acagtttca caacaccca 
      tccaattgt gaaaaagtt ccgtttacg atcgaaaat 
      gagcggcaa tcgcaaaac ttcccctcg ctcttttat 
      tttcggtcg gtttgttat ccttatcgg tggtattcc 
      gacccctct ggctcgggg acccttggt aacacccaa 
      acttttcgt tgttgaaag cattagtat tctcggagc 
      gaattcggg aaggcagtt tcgatgtcg tgcgacaat 
      agagggtag gtttcgcgg cagcgaccc tccggactc 
      cggaggttc agtcttgag acgggtgcg tgtggctcc 
      gggtcttcg atggctatc ggagatgga ttttaataa 
      cgcgggttt tttcgtttg gtggccgtt taatgcgag 
      atttttgat ggatgtggc caatcaaat tcggcagtc 
      gttcgtcgt cgaatcgac ggttccgtt tcgttttct 
      ctcactccg acacacgtc tcttctccc tctctctct 
      catttcgag ttctttgtt cggcgcgtt cacacgtgt 
      tgtgcttca gttaccgct caggcatgc caaccgtcg 
      gtgtaacgt cacgtccgt tggtgtttt agtgtcctt 
      ttttcaaca ggggaggaa aaaaatgga atacgccag 
      gcggaagta caaaagcgg catccgcag cagcgcagt 
      gaggagcag cgtggtagc cagcggagg cggcggcgg 
      cggcggcgg cgggggcag cggtaggct gtgcgaagt 
      gaggcgcgc atacaaagc ggccgtggt gccgtgcaa 
      aacgggccg tcgcagggc ccggggcat gagcatggg 
      ccagaaggt gtcaggagt caaggctgt taaccggga 
      tgcagccgc accctacaa gcccgtcat cccaaggga 
      actggcgca ggatttcgc tcgtccacc gcggctgga 
      catgctgct cgacatgcc tgcagctct cagggagac 
      ccaactgaa gcacagttg gaaccagga cgaccgctc 
      tctcaacat cttcgtcaa agaggacga caaactgac 
      cttccatag acatcccgt ggcgcaaag cacggactg 
      tatacgagg caaagtcgg cttcacgaa gggcatgca 
      ctgttggga ggtgtgttg gtcgacgag gcagcgagg 
      cacgcatgc agtggtggg cgtcgcgac ggccgaggc 
      gccgctgca ttcggtcgg ataccagag cctagtcgg 
      cagcacgga cacgtcgtg ggggtggga tttaggtcg 
      aaataaact ctaccacga ttcgaaaaa ctgtcccgg 
      tgtgacgta cccgacttt attaaagcc ggacgagac 
      tttcatagt gcccgataa gtttttagt ggtgttaga 
      tatggatga ggggacact tagttttgt tgtggacgg 
      ccagtactt gggagtagc gttcagggg actgaaagg 
      gaggaagtt gtatccaat cgttagtgc cgtttgggg 
      tcactgtga gatcaccat gaggtacat tggaggact 
      tgatccgga gcctttgcc gctgatgga cctctgccg 
      gcgcgtgat ccggcagcg cttaggcaa gcagcacct 
      cgaggagaa agtgatgaa cctgcagct gccccaagc 
      tctacagac ttacctctt gtacagaga tcgcagata 
      ataacagtg cttcttagt gcagtgaag tgtcggaca 
      aaattttat tatcatccg atcgtgtgt cagctgtca 
      ggtgtagag ctaaaccaa ccaaaaaga atctaaaaa 
      ttgacggag gaatgtgtc tcagagcga gcgagaggg 
      agagaaagt acgacgcgg acgtatttc gtttcggac 
      gatttttat tatgatttc gttaattgg acacactca 
      agtcttcgc gctagttct atatttgtg ctgtgaatg 
      gtacgattg atttctttc ctcatgatt tcgattgaa 
      acaaacaaa taacggaaa cttcctgat ctcgggaca 
      agccaactt gagagaaca atgccataa ggtgtacat 
      agttgtaac tcagtcttc ttaacttat attctcggc 
      acggcttcc tgatatagc tttacaaaa tagattttt 
      aaacttttt tgggtcaag tgtactcga ttcgaggat 
      ggttgtata cagggagcg tcagcgaat acaacgaat 
      cgagggcgt gccttcggt ggcgccctc tgtgttgac 
      ttttgtaca ttcgtcgga gctgaatgg aattacacc 
      aagataata ttactgtat gtaagaata ttagattat 
      ttttgtaaa tatttagtt agtgccagt ctcattaca 
      ccataattt atgtctgat gcgaacaat cgactgctc 
      tgttctatg agattaatg ataattttt aagagtgta 
      taagatttt ttctttttt tgcattatt gctgaatcc 
      gggccatct aatggcctg tcgtgaggt ttagaatgt 
      cggagtgta tgtaattgt gtattgtgt tagttttta 
      gattgttta ttaagaaat ttcaaaaca ttacgtgtt 
      cattagata aatttttgc aattattgt ttttactgt 
      ttttttctt ttttcgttt tatggtttt aaacaagca 
      aaagttctc gaatcgttt tggtgatag atttcgacc 
      agatttttt tggtcttgt tagaatatt gaaaatgca 
      atgcgtagt ggcctggac tactatgat attgataag 
      tagtatgtt ttattgtat tctaagttt cgctgtgaa 
      tgtccaggc ctatatttc agttactcg atgtatgat 
      tatactaaa taataataa gtaaactct tta
    • >TC034075 RC
      acaattggc catcagtaa cagcgcaca gttatgttt 
      atgtagata taagacggt cttaaagac tgtgttatt 
      tctttattt tattgttaa ttgacgcaa agttaatta 
      acgcgcctg ttgtgatag ccagttttt gggccaagt 
      gcaccagtg ctccatttt ttgcgtgtt ttgttaccc 
      aacgttaca aatcggagg acgaggatg aggcatccg 
      aaaatcctg acgtacaat ccgcggatt tattaggtg 
      agtgtcgaa aatttcagt ttttaataa atcccgatt 
      agccgaatt aaatcgggc ttttggcgc ggtgccatt 
      ttaatcgag ggagggcca ccctatgac atacattat 
      atgggttaa gcctaaaag tggtagttc atcgacctc 
      attctcccc ctttcataa ttttaatta caaataatg 
      aaatttatg gaggcacac aaatataag tttgtcgga 
      tatggggtg cgcccggcg ctccgaatt cgaaccttg 
      gcgctccga aatccggcg ttctattgg ctcagaggg 
      tccgcgcct ccctcttcg aaccaatgg ggctcccgg 
      tgcggcttc ggaccgttt cttgccaag gttagactt 
      cagtgttgg cgagcagcg gacatgata acttctccc 
      ctgctcgga ctctgttgg ctgcctaat aaccgtgtg 
      tttagtgta caatccgct cacaataag atactcgga 
      cgtttagtg tggaaccaa catcagtgt tttttgaca 
      ataggacaa gcgcaatct gtgactccg tatgtcgga 
      ttttggaat agtgtcgct atttttcaa ccatcatca 
      caccccttt aatatggaa acaagaagc gaaaatagc 
      gcgccccat ttgccttta ccccctgag agctacctg 
      cacattttt ccaccaaac acctcgacc gtaccactt 
      tctttacag gccaaggcg attcttgag gtcgcacag 
      tttcacaac acccatcca attgtgaaa aagttccgt 
      ttacgatcg aaaatgagc ggcaatcgc aaaacttcc 
      cctcgctct tttattttc ggtcggttt gttatcctt 
      atcggtggt attccgacc cctctggct cggggaccc 
      ttggtaaca cccaaactt ttcgttgtt gaaagcatt 
      agtattctc ggagcgaat tcgggaagg cagtttcga 
      tgtcgtgcg acaatagag ggtaggttt cgcggcagc 
      gaccctccg gactccgga ggttcagtc ttgagacgg 
      gtgcgtgtg gctccgggt cttcgatgg ctatcggag 
      atggatttt aataacgcg ggttttttc gtttggtgg 
      ccgtttaat gcgagattt ttgatggat gtggccaat 
      caaattcgg cagtcgttc gtcgtcgaa tcgacggtt 
      ccgtttcgt tttctctca ctccgacac acgtctctt 
      ctccctctc tctctcatt tcgagttct ttgttcggc 
      gcgttcaca cgtgttgtg cttcagtta ccgctcagg 
      catgccaac cgtcggtgt aacgtcacg tccgttggt 
      gttttagtg tcctttttt caacagggg aggaaaaaa 
      atggaatac gccaggcgg aagtacaaa agcggcatc 
      cgcagcagc gcagtgagg agcagcgtg gtagccagc 
      ggaggcggc ggcggcggc ggcggcggg ggcagcggt 
      aggctgtgc gaagtgagg cgcgcatac aaagcggcc 
      gtggtgccg tgcaaaacg ggccgtcgc agggcccgg 
      ggcatgagc atgggccag aaggtgtca ggagtcaag 
      gctgttaac cgggatgca gccgcaccc tacaagccc 
      gtcatccca agggaactg gcgcaggat ttcgctcgt 
      ccaccgcgg ctggacatg ctgctcgac atgcctgca 
      gctctcagg gagacccaa ctgaagcac agttggaac 
      caggacgac cgctctctc aacatcttc gtcaaagag 
      gacgacaaa ctgaccttc catagacat cccgtggcg 
      caaagcacg gactgtata cgaggcaaa gtcggcttc 
      acgaagggc atgcactgt tgggaggtg tgttggtcg 
      acgaggcag cgaggcacg catgcagtg gtgggcgtc 
      gcgacggcc gaggcgccg ctgcattcg gtcggatac 
      cagagccta gtcggcagc acggacacg tcgtggggg 
      tgggattta ggtcgaaat aaactctac cacgattcg 
      aaaaactgt cccggtgtg acgtacccg actttatta 
      aagccggac gagactttc atagtgccc gataagttt 
      ttagtggtg ttagatatg gatgagggg acacttagt 
      tttgttgtg gacggccag tacttggga gtagcgttc 
      aggggactg aaagggagg aagttgtat ccaatcgtt 
      agtgccgtt tggggtcac tgtgagatc accatgagg 
      tacattgga ggacttgat ccggagcct ttgccgctg 
      atggacctc tgccggcgc gtgatccgg cagcgctta 
      ggcaagcag cacctcgag gagaaagtg atgaacctg 
      cagctgccc caagctcta cagacttac ctcttgtac 
      agagatcgc agataataa cagtgcttc ttagtgcag 
      tgaagtgtc ggacaaaat tttattatc atccgatcg 
      tgtgtcagc tgtcaggtg tagagctaa accaaccaa 
      aaagaatct aaaaattga cggaggaat gtgtctcag 
      agcgagcga gagggagag aaagtacga cgcggacgt 
      atttcgttt cggacgatt tttattatg atttcgtta 
      attggacac actcaagtc ttcgcgcta gttctatat 
      ttgtgctgt gaatggtac gattgattt ctttcctca 
      tgatttcga ttgaaacaa acaaataac ggaaacttc 
      ctgatctcg ggacaagcc aacttgaga gaacaatgc 
      cataaggtg tacatagtt gtaactcag tcttcttaa 
      cttatattc tcggcacgg cttcctgat atagcttta 
      caaaataga tttttaaac ttttttggg tcaagtgta 
      ctcgattcg aggatggtt gtatacagg gagcgtcag 
      cgaatacaa cgaatcgag ggcgtgcct tcggtggcg 
      ccctctgtg ttgactttt gtacattcg tcggagctg 
      aatggaatt acaccaaga taatattac tgtatgtaa 
      gaatattag attattttt gtaaatatt tagttagtg 
      ccagtctca ttacaccat aatttatgt ctgatgcga 
      acaatcgac tgctctgtt ctatgagat taatgataa 
      tttttaaga gtgtataag attttttct ttttttgca 
      ttattgctg aatccgggc catctaatg gcctgtcgt 
      gaggtttag aatgtcgga gtgtatgta attgtgtat 
      tgtgttagt ttttagatt gtttattaa gaaatttca 
      aaacattac gtgttcatt agataaatt tttgcaatt 
      attgttttt actgttttt ttctttttt cgttttatg 
      gttttaaac aagcaaaag ttctcgaat cgttttggt 
      gatagattt cgaccagat ttttttggt cttgttaga 
      atattgaaa atgcaatgc gtagtggcc tggactact 
      atgatattg ataagtagt atgttttat tgtattcta 
      agtttcgct gtgaatgtc caggcctat atttcagtt 
      actcgatgt atgattata ctaaataat aataagtaa 
      actcttta
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