Status
Please wait ...
Gene information
General information
TC010924 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1340900..1342252
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010924
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07269

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    egg chamber structure irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber mostly fused - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    10 larvae on the slide and all of them showing empty egg with some other phenotype.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010924 RA
      MKKKVLLMG KSGSGKTSM RSIIFANYI ARDTRRLGA 
      TIDVEHSHV RFLGNLVLN LWDCGGQEA FMENYFASQ 
      RDNIFRNVE VLIYVFDVE SRELEKDMH YYQSCLEAI 
      LQNSPEAKV FCLIHKMDL VTEDQREII FKEREADLK 
      RLSLPLECT CFRTSIWDE TLYRAWSSI VYMLIPNVK 
      ELENSLQQF ANIVDADEV LLFERATFL VISHCQRKH 
      HRDVHRFEK VSNIIKQFK LSCSKLAAQ FQSMEVRNS 
      AFAAYIDMF TSNTYVMVC MSDPQIPSE VTLINIRNA 
      RKHFEKLEK VSSSAPAIG HR
    CDS sequence
    • >TC010924 RA
      atgaagaag aaggtcctg ttaatgggt aaaagcggc 
      tccggcaag actagcatg aggtcaata attttcgcc 
      aattacatc gccagagac acgcggagg ttgggggcc 
      accatcgac gtggagcac tcacacgtc cgatttttg 
      ggcaacttg gtcctgaat ttgtgggac tgtgggggt 
      caggaggcc ttcatggag aactatttc gcgtcacag 
      cgtgacaat attttcaga aatgtggag gtccttatc 
      tatgttttc gacgtggaa agccgggag ttggagaaa 
      gacatgcac tactaccag tcgtgtctt gaggcgatt 
      ttacaaaac tcaccagag gctaaagtg ttctgctta 
      atccataaa atggatctt gtgactgag gaccaaaga 
      gaaattatt ttcaaagaa cgcgaggcg gatttaaaa 
      cgattgtca ctaccactt gaatgtact tgctttagg 
      acttcaatc tgggatgag actttgtac agagcttgg 
      tcaagtatt gtgtacatg ttgattccc aacgtcaaa 
      gaactggaa aatagtctg caacagttc gcgaatatt 
      gtcgacgct gatgaggtg ctgttgttt gaaagggca 
      acatttttg gtgatctcg cactgccag agaaaacat 
      caccgagac gtacaccgg tttgaaaaa gtctcaaat 
      ataataaaa cagttcaaa ttgtcgtgc tccaaactt 
      gcggcgcaa tttcagagc atggaagtt cgaaattcg 
      gcgtttgcg gcctatatt gacatgttc actagcaat 
      acttacgta atggtgtgc atgtctgac ccgcagatt 
      ccgtcagaa gtgactcta atcaatatt cggaatgct 
      aggaaacat ttcgagaag ttggagaag gtttcgagc 
      tcagcgccg gccattgga caccggtag
    mRNA sequence
    • >TC010924 RA
      cgcatttga cgttttgat tttgatata tcgcgtcct 
      gtgtttaga gtatagatt acaaatttt attttgagt 
      caacaaaac ggccacaaa atgaagaag aaggtcctg 
      ttaatgggt aaaagcggc tccggcaag actagcatg 
      aggtcaata attttcgcc aattacatc gccagagac 
      acgcggagg ttgggggcc accatcgac gtggagcac 
      tcacacgtc cgatttttg ggcaacttg gtcctgaat 
      ttgtgggac tgtgggggt caggaggcc ttcatggag 
      aactatttc gcgtcacag cgtgacaat attttcaga 
      aatgtggag gtccttatc tatgttttc gacgtggaa 
      agccgggag ttggagaaa gacatgcac tactaccag 
      tcgtgtctt gaggcgatt ttacaaaac tcaccagag 
      gctaaagtg ttctgctta atccataaa atggatctt 
      gtgactgag gaccaaaga gaaattatt ttcaaagaa 
      cgcgaggcg gatttaaaa cgattgtca ctaccactt 
      gaatgtact tgctttagg acttcaatc tgggatgag 
      actttgtac agagcttgg tcaagtatt gtgtacatg 
      ttgattccc aacgtcaaa gaactggaa aatagtctg 
      caacagttc gcgaatatt gtcgacgct gatgaggtg 
      ctgttgttt gaaagggca acatttttg gtgatctcg 
      cactgccag agaaaacat caccgagac gtacaccgg 
      tttgaaaaa gtctcaaat ataataaaa cagttcaaa 
      ttgtcgtgc tccaaactt gcggcgcaa tttcagagc 
      atggaagtt cgaaattcg gcgtttgcg gcctatatt 
      gacatgttc actagcaat acttacgta atggtgtgc 
      atgtctgac ccgcagatt ccgtcagaa gtgactcta 
      atcaatatt cggaatgct aggaaacat ttcgagaag 
      ttggagaag gtttcgagc tcagcgccg gccattgga 
      caccggtag gaagcaagg ggaaagtct tgtgccaaa 
      tgaagagta caattaagg ttccaattc agcatatcc 
      ggatttgta atgtctgca atacttgaa atattaatt 
      ttatttatt gtaataagg tgctataaa ctatagcta 
      tgtgctata taaaagaga ttaatatcc aaacggtat 
      tgtattaat attttcgag aataacccc attcttctg 
      tttctattt taattaaaa gttctttag tttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY