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Gene information
General information
TC010923 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1323090..1328417
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010923
    iBeetle screen iB_07268

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010923 RA
      MADKVDKAE LRNRLTPIQ YHVTQEKGT 
      ERPFTGCYN KVYDAGTYV CIVCEQPLF 
      SSETKYDSG CGWPAFNDV LDQGKVKLT 
      PDTSGGRIR TEVTCAQCG AHLGHVFND 
      GPMPTRKRF CINSASLNF IPDKKEGGD 
      S
    • >TC010923 RB
      MHKHTGGLL GRSGRFLVY SLSRKFVNN 
      TPVVRMADK VDKAELRNR LTPIQYHVT 
      QEKGTERPF TGCYNKVYD AGTYVCIVC 
      EQPLFSSET KYDSGCGWP AFNDVLDQG 
      KVKLTPDTS GGRIRTEVT CAQCGAHLG 
      HVFNDGPMP TRKRFCINS ASLNFIPDK 
      KEGGDS
    CDS sequence
    • >TC010923 RA
      atggccgat aaagtggat aaggccgag ttgaggaat 
      cggctcact cccatacag taccatgtg acccaagaa 
      aagggcact gagcgaccg ttcacaggg tgttacaat 
      aaagtctac gacgcgggc acttacgtc tgcatcgtc 
      tgtgaacaa ccactgttc agttcggag acgaaatac 
      gacagtggt tgcggctgg cctgccttc aacgatgtc 
      ttagaccaa ggcaaagtc aaattgaca ccggacacc 
      agtggaggg cggataaga accgaagtg acttgtgcc 
      caatgcggt gcccatctc ggtcacgtg ttcaacgat 
      ggacccatg cccacgagg aagaggttc tgtataaat 
      tcggcctct cttaatttc atcccggat aaaaaggag 
      ggtggtgat agctag
    • >TC010923 RB
      atgcataaa cacacgggg ggactgttg ggtagatcg 
      ggccgcttt ttagtctac agtttgagc aggaaattc 
      gtaaataat acaccagtg gtgaggatg gccgataaa 
      gtggataag gccgagttg aggaatcgg ctcactccc 
      atacagtac catgtgacc caagaaaag ggcactgag 
      cgaccgttc acagggtgt tacaataaa gtctacgac 
      gcgggcact tacgtctgc atcgtctgt gaacaacca 
      ctgttcagt tcggagacg aaatacgac agtggttgc 
      ggctggcct gccttcaac gatgtctta gaccaaggc 
      aaagtcaaa ttgacaccg gacaccagt ggagggcgg 
      ataagaacc gaagtgact tgtgcccaa tgcggtgcc 
      catctcggt cacgtgttc aacgatgga cccatgccc 
      acgaggaag aggttctgt ataaattcg gcctctctt 
      aatttcatc ccggataaa aaggagggt ggtgatagc 
      tag
    mRNA sequence
    • >TC010923 RA
      ttaggcgtg tcaggcgta attagcgtt tattggggt 
      tattacgtt aattgggtg ctcaattta cataattgt 
      gtttataat ttgcgaagt agtggttgc ggtaaagtg 
      aggttaggt ccagtgtca aaataatgt cgcttacgt 
      aacgtggaa attcggtca attttgtat aaatattgg 
      cgcaactaa tcgtgtccg cgctcaaat gtgatgcat 
      aaacacacg gggggactg ttgggtaga tcgggccgc 
      tttttagtc tacagtttg agcaggaaa ttcgtaaat 
      aatacacca ggtgtttgt cactcgttt cgcataaca 
      ctttttatt ttgtgctgc actagtggt gaggatggc 
      cgataaagt ggataaggc cgagttgag gaatcggct 
      cactcccat acagtacca tgtgaccca agaaaaggg 
      cactgagcg accgttcac agggtgtta caataaagt 
      ctacgacgc gggcactta cgtctgcat cgtctgtga 
      acaaccact gttcagttc ggagacgaa atacgacag 
      tggttgcgg ctggcctgc cttcaacga tgtcttaga 
      ccaaggcaa agtcaaatt gacaccgga caccagtgg 
      agggcggat aagaaccga agtgacttg tgcccaatg 
      cggtgccca tctcggtca cgtgttcaa cgatggacc 
      catgcccac gaggaagag gttctgtat aaattcggc 
      ctctcttaa tttcatccc ggataaaaa ggagggtgg 
      tgatagcta ggagggacc actaaccaa tcaaagtga 
      taatactaa ctagttagg cctactgtt tttatttag 
      tcgtgtgat caactaatc gttgaagcc aaactcgaa 
      tttcttacc tcgaattgt ttttgttag cttttgtgt 
      tgtgtattt atgcatatc gaattaaca ttttttaag 
      aggctttac attttgtga aacaataaa tttgataga 
      aaagc
    • >TC010923 RB
      ttaggcgtg tcaggcgta attagcgtt tattggggt 
      tattacgtt aattgggtg ctcaattta cataattgt 
      gtttataat ttgcgaagt agtggttgc ggtaaagtg 
      aggttaggt ccagtgtca aaataatgt cgcttacgt 
      aacgtggaa attcggtca attttgtat aaatattgg 
      cgcaactaa tcgtgtccg cgctcaaat gtgatgcat 
      aaacacacg gggggactg ttgggtaga tcgggccgc 
      tttttagtc tacagtttg agcaggaaa ttcgtaaat 
      aatacacca gtggtgagg atggccgat aaagtggat 
      aaggccgag ttgaggaat cggctcact cccatacag 
      taccatgtg acccaagaa aagggcact gagcgaccg 
      ttcacaggg tgttacaat aaagtctac gacgcgggc 
      acttacgtc tgcatcgtc tgtgaacaa ccactgttc 
      agttcggag acgaaatac gacagtggt tgcggctgg 
      cctgccttc aacgatgtc ttagaccaa ggcaaagtc 
      aaattgaca ccggacacc agtggaggg cggataaga 
      accgaagtg acttgtgcc caatgcggt gcccatctc 
      ggtcacgtg ttcaacgat ggacccatg cccacgagg 
      aagaggttc tgtataaat tcggcctct cttaatttc 
      atcccggat aaaaaggag ggtggtgat agctaggag 
      ggaccacta accaatcaa agtgataat actaactag 
      ttaggccta ctgttttta tttagtcgt gtgatcaac 
      taatcgttg aagccaaac tcgaatttc ttacctcga 
      attgttttt gttagcttt tgtgttgtg tatttatgc 
      atatcgaat taacatttt ttaagaggc tttacattt 
      tgtgaaaca ataaatttg atagaaaag c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY