Status
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Gene information
General information
TC032993
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013892
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 304777..311844
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032993
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07264

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032993 RA
      MKITVAVSA CFYILAIQK SESKLFNPS LVVSFIKPT 
      IVDLIDKSV EESRCASDL KQFITDLFK LKWWAIRMF 
      DASSKLHPG FFNGNFRQF GNYDQCLSV SKTLNGGEI 
      SGQYCTVSF QAFENTTKV FFKMVKNIS QSRGTGKMI 
      DWKFFLKYT KAFLGLCLP KSCSIKDMN RLWSYIQRV 
      FRIPVEAKF LEGACVSGR EQYASADDF YVFIFFGVI 
      LAILLSSTI YDLFLQSNK GQPDVFVCF SLYTNTKRL 
      LSTRCENDS LTCLYGIRV ISMAWVIIG HIAVIQMFS 
      SNINTLYVV TEWKTSYMN TFITAAPYA VDTFFSISG 
      LLVVYIHLK YSEKFKINL WAFYLHRVI RLVPALVAT 
      LLIYTSLYK YVSEGPLWP LIVKKLQVP CQYTWWATL 
      LFFNNYLDF NNQCMQHTW YLSVDTQLY VIVAPIILY 
      YMKKNPSKT LLGITLACL FSILYTFLM VVHYNLGFI 
      PYWEMEDND SKITYFNPL SRMPSWLVG SVLGYLLYQ 
      NKKLQNPQK INFLLWTAS LLLMLGLIL YHQVFMNSP 
      HNVFRSAFF NSFSRFMWS LAVCFIIFS CSTGRGDFI 
      NNFLSHPVF IVLGKLTYS MYLTHVLII MAVTGNQKH 
      ASYFSNFGV FCDFCSNSV IIIGCSTLM CLCFETPFI 
      SILKHLDKR KNIKHS
    CDS sequence
    • >TC032993 RA
      atgaaaata actgttgcc gtttcagcc tgtttctac 
      attcttgca attcaaaaa tccgaatca aaattattt 
      aacccatct ctagttgtc agttttatc aagccaaca 
      attgtagat ttgattgat aaaagtgtt gaagaatca 
      cgatgtgcc tcagaccta aaacaattc atcaccgac 
      ttgttcaaa ctcaaatgg tgggcgatt cgaatgttt 
      gatgctagc agtaaattg catcctgga ttttttaat 
      ggcaatttt cgccaattt ggaaactat gaccaatgt 
      ctcagtgtg tctaaaact ttgaatggg ggagaaata 
      agtgggcaa tattgcacg gtttcattc caggcattc 
      gaaaatacg acaaaagtg tttttcaag atggtcaaa 
      aatattagt caaagcaga gggactgga aaaatgatt 
      gattggaaa tttttcctc aaatatact aaagctttt 
      ctaggtctt tgtctcccc aaatcatgc tcaatcaaa 
      gatatgaat aggttgtgg agctacata cagagagtt 
      tttcgtatt ccagttgag gcgaagttt ttggaaggc 
      gcatgtgtt tctggaagg gagcaatac gcaagtgct 
      gatgatttt tacgtcttc atatttttt ggggtgata 
      ttggccata cttttatca agcacgatt tatgatttg 
      tttttgcaa agtaataag ggacaaccg gacgttttt 
      gtatgtttt tctctctat acaaacact aaacgactt 
      ttgtcaaca cgttgcgaa aatgatagt ctcacttgt 
      ttgtatgga attcgagtc attagtatg gcatgggtc 
      atcattggc catattgca gtcatccaa atgttttcc 
      tccaatatt aacactctc tacgttgtc acagagtgg 
      aaaactagt tatatgaac acttttatc accgcagcc 
      ccttacgca gtcgatacg tttttttca attagtggt 
      cttttggta gtttacatc catttgaaa tactcagaa 
      aagttcaag atcaatctg tgggctttc tatttacac 
      agagtaata aggctagtt ccagcacta gttgcaacc 
      cttttaatt tacacaagc ttgtacaaa tacgtaagt 
      gaagggcca ctatggcct ttaatcgtc aaaaaatta 
      caagtgcct tgtcaatac acatggtgg gcaacttta 
      ctttttttc aataattat cttgatttt aataatcag 
      tgtatgcaa cacacttgg tacttatca gtggacacc 
      caactgtat gtgatagta gcaccaatc atcctgtat 
      tacatgaag aaaaaccca tcaaaaact ctattagga 
      attactttg gcttgctta ttttcaatt ttatacact 
      tttttaatg gttgttcat tacaatctt ggatttata 
      ccatactgg gaaatggaa gacaatgat tcaaaaata 
      acgtatttt aatccctta tcacgtatg ccttcctgg 
      cttgttggc agtgttttg ggttactta ctctaccaa 
      aataagaag ctccaaaat cctcaaaaa attaacttc 
      ttgctatgg acagcctcg ttgctactt atgttagga 
      ctaattttg tatcatcaa gtcttcatg aattctcca 
      cacaacgtg tttcgatca gccttcttt aacagcttt 
      tcccggttc atgtggtct ttagccgtt tgttttatt 
      atttttagt tgcagtacc ggacgtgga gattttatc 
      aacaatttt ttgtctcat cccgtattc atcgttttg 
      ggaaaactt acctacagt atgtatctc actcacgta 
      ctgattata atggctgtg acagggaac caaaaacat 
      gctagttat tttagcaat tttggagtg ttttgtgac 
      ttttgtagc aattcggtg atcattatt ggatgttcg 
      actttaatg tgcttgtgt ttcgagaca ccgtttata 
      tccatttta aaacatttg gataaacgg aaaaatatt 
      aaacactcg tag
    mRNA sequence
    • >TC032993 RA
      atcccatat caacttgaa ctcatatct aatggtatg 
      ttaagtgtt cacatttac tttcaaata attagtaat 
      tttaattat tgtcaaaac gataggtaa aagttgata 
      aaagccgat aaaaataca cttcttaaa aacgaatag 
      aacgacagt cgcaatgaa aataactgt tgccgtttc 
      agcctgttt ctacattct tgcaattca aaaatccga 
      atcaaaatt atttaaccc atctctagt tgtcagttt 
      tatcaagcc aacaattgt agatttgat tgataaaag 
      tgttgaaga atcacgatg tgcctcaga cctaaaaca 
      attcatcac cgacttgtt caaactcaa atggtgggc 
      gattcgaat gtttgatgc tagcagtaa attgcatcc 
      tggattttt taatggcaa ttttcgcca atttggaaa 
      ctatgacca atgtctcag tgtgtctaa aactttgaa 
      tgggggaga aataagtgg gcaatattg cacggtttc 
      attccaggc attcgaaaa tacgacaaa agtgttttt 
      caagatggt caaaaatat tagtcaaag cagagggac 
      tggaaaaat gattgattg gaaattttt cctcaaata 
      tactaaagc ttttctagg tctttgtct ccccaaatc 
      atgctcaat caaagatat gaataggtt gtggagcta 
      catacagag agtttttcg tattccagt tgaggcgaa 
      gtttttgga aggcgcatg tgtttctgg aagggagca 
      atacgcaag tgctgatga tttttacgt cttcatatt 
      ttttggggt gatattggc catactttt atcaagcac 
      gatttatga tttgttttt gcaaagtaa taagggaca 
      accggacgt ttttgtatg tttttctct ctatacaaa 
      cactaaacg acttttgtc aacacgttg cgaaaatga 
      tagtctcac ttgtttgta tggaattcg agtcattag 
      tatggcatg ggtcatcat tggccatat tgcagtcat 
      ccaaatgtt ttcctccaa tattaacac tctctacgt 
      tgtcacaga gtggaaaac tagttatat gaacacttt 
      tatcaccgc agcccctta cgcagtcga tacgttttt 
      ttcaattag tggtctttt ggtagttta catccattt 
      gaaatactc agaaaagtt caagatcaa tctgtgggc 
      tttctattt acacagagt aataaggct agttccagc 
      actagttgc aaccctttt aatttacac aagcttgta 
      caaatacgt aagtgaagg gccactatg gcctttaat 
      cgtcaaaaa attacaagt gccttgtca atacacatg 
      gtgggcaac tttactttt tttcaataa ttatcttga 
      ttttaataa tcagtgtat gcaacacac ttggtactt 
      atcagtgga cacccaact gtatgtgat agtagcacc 
      aatcatcct gtattacat gaagaaaaa cccatcaaa 
      aactctatt aggaattac tttggcttg cttattttc 
      aattttata cactttttt aatggttgt tcattacaa 
      tcttggatt tataccata ctgggaaat ggaagacaa 
      tgattcaaa aataacgta ttttaatcc cttatcacg 
      tatgccttc ctggcttgt tggcagtgt tttgggtta 
      cttactcta ccaaaataa gaagctcca aaatcctca 
      aaaaattaa cttcttgct atggacagc ctcgttgct 
      acttatgtt aggactaat tttgtatca tcaagtctt 
      catgaattc tccacacaa cgtgtttcg atcagcctt 
      ctttaacag cttttcccg gttcatgtg gtctttagc 
      cgtttgttt tattatttt tagttgcag taccggacg 
      tggagattt tatcaacaa ttttttgtc tcatcccgt 
      attcatcgt tttgggaaa acttaccta cagtatgta 
      tctcactca cgtactgat tataatggc tgtgacagg 
      gaaccaaaa acatgctag ttattttag caattttgg 
      agtgttttg tgacttttg tagcaattc ggtgatcat 
      tattggatg ttcgacttt aatgtgctt gtgtttcga 
      gacaccgtt tatatccat tttaaaaca tttggataa 
      acggaaaaa tattaaaca ctcgtagtt gtatgagta 
      aaacattaa aaaattgcg gccttagac acggaacta 
      ttttatttt gagtttatt tctatcatc agtgatgaa 
      gtaatttgt taaaaacca cttaagtac gttaaccat 
      gaataactg ttaccttga gtgttaata acaaacact 
      atgctgttt gatataatt atcatttca agatcctga 
      acggataca tctttttac ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY