Status
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Gene information
General information
TC007002 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12192540..12202675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007002
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07262

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007002 RA
      MQVRLMCEL PKREKTTLR SAFLVVFIL LVLIAWIYD 
      IFASPDFKD TKFAKSKFG KFISIFSLT QNWHRLNKI 
      DLTNPDIRS LRCLQGIRF FCMYNVIAA HCVAANLSI 
      PVVNTRYTE EISERVADR WVGSGSRHA ILQTYFMLS 
      SWLLTYQFF VDHEKKEKI KFSYLVNSF VKRYFRITP 
      TLLILVGMC ATWLVHLSR GPFWDDIIG HSYRTCRSN 
      WWSNPIYLN NFIHPVEMC QPSLWYIAV DTQYYIVGL 
      FILCLIWKY QEKIALILG CLWVVHVLD TYSRIYSSK 
      IILPIYPEL FYNMKFILW DHAGSVAKA PIANMSGLI 
      AGLGFGYTF YKCRNKKLF TKRIHVILW WFASLGVSI 
      LIIPIPALF KKTTIFSNN PEVNFAIYA CLSRSIYIF 
      GTGFFIFGM THGQGHIIR AINEWPPMY VLGRLTYSA 
      YLLHVPIQL VKAGLKRAP NYLNDFILN WEMLSDLFL 
      SYMAALFFT LFFELPISE LKKHITSML KNLRRNEVT 
      CYSNKTEKC E
    CDS sequence
    • >TC007002 RA
      atgcaagtt cgcctaatg tgcgaattg ccaaaaagg 
      gagaagacg acactccgt agtgcattt cttgtcgta 
      tttatccta cttgtgctg atagcgtgg atctacgac 
      atttttgca tctcctgac ttcaaagac acaaaattt 
      gccaaatcg aaatttgga aagtttatc tcaatattt 
      tccctaacc caaaactgg catcgactt aataaaatc 
      gatctcacc aatcctgat atcagatca ttgcgatgt 
      ctgcaaggc atccgtttt ttctgcatg tacaacgtt 
      attgctgca cattgtgtg gctgctaat ttatctatt 
      ccagttgta aataccaga tatacagag gagatttcc 
      gaacgtgtg gctgatcga tgggttgga agtggctct 
      cgacacgct atacttcaa acttatttt atgttgtca 
      tcgtggctg ctaacgtat cagtttttt gtggatcat 
      gaaaagaaa gaaaaaatc aaattttcc tatttggtc 
      aattcgttt gtgaagaga tacttcaga ataactccc 
      actttgctc atccttgtt ggtatgtgt gcgacgtgg 
      ttggtccat ctatcacgt ggtccgttt tgggacgac 
      attattggt cattcctac agaacctgc cgttccaat 
      tggtggagc aatcccata tatttgaac aatttcata 
      catccagtc gaaatgtgt caaccctct ctatggtat 
      atagctgtc gatacacaa tactatata gtgggcctg 
      tttattttg tgtctcata tggaagtac caagaaaaa 
      attgcacta atattaggc tgcctttgg gtagtacac 
      gtccttgat acatattct cgaatctat agttcaaaa 
      attatttta ccaatttat ccagagttg ttctacaac 
      atgaaattt atactatgg gaccatgct ggttcagta 
      gctaaagcc cctattgct aatatgtca ggtctgatc 
      gctggttta ggttttggc tacactttt tacaaatgt 
      agaaataaa aaattattt accaaaagg attcatgtt 
      attttatgg tggtttgca agtctcgga gtttcaata 
      ttgataatc ccgattcca gcactattt aaaaaaaca 
      acaatattt tcaaataac cctgaagtt aattttgcg 
      atatatgct tgtttaagc agaagtatc tatattttt 
      gggacgggt ttttttatt tttggaatg acacacgga 
      caaggacat ataataaga gcaataaat gagtggcct 
      cctatgtac gttcttggc cgcctcact tacagtgct 
      tacctttta cacgttccc atccagttg gtgaaagct 
      ggcttgaaa agagcacca aattatttg aatgacttc 
      attttgaat tgggagatg ctttcggat cttttcctt 
      tcctacatg gcggcgttg ttttttaca cttttcttt 
      gagctgccg attagtgag ttaaaaaaa cacattact 
      tcaatgttg aaaaatcta aggagaaat gaggttact 
      tgttacagc aataaaaca gaaaagtgt gaataa
    mRNA sequence
    • >TC007002 RA
      gagaggaaa tcgagtttt ttccagaga tgcaagttc 
      gcctaatgt gcgaattgc caaaaaggg agaagacga 
      cactccgta gtgcatttc ttgtcgtat ttatcctac 
      ttgtgctga tagcgtgga tctacgaca tttttgcat 
      ctcctgact tcaaagaca caaaatttg ccaaatcga 
      aatttggaa agtttatct caatatttt ccctaaccc 
      aaaactggc atcgactta ataaaatcg atctcacca 
      atcctgata tcagatcat tgcgatgtc tgcaaggca 
      tccgttttt tctgcatgt acaacgtta ttgctgcac 
      attgtgtgg ctgctaatt tatctattc cagttgtaa 
      ataccagat atacagagg agatttccg aacgtgtgg 
      ctgatcgat gggttggaa gtggctctc gacacgcta 
      tacttcaaa cttatttta tgttgtcat cgtggctgc 
      taacgtatc agttttttg tggatcatg aaaagaaag 
      aaaaaatca aattttcct atttggtca attcgtttg 
      tgaagagat acttcagaa taactccca ctttgctca 
      tccttgttg gtatgtgtg cgacgtggt tggtccatc 
      tatcacgtg gtccgtttt gggacgaca ttattggtc 
      attcctaca gaacctgcc gttccaatt ggtggagca 
      atcccatat atttgaaca atttcatac atccagtcg 
      aaatgtgtc aaccctctc tatggtata tagctgtcg 
      atacacaat actatatag tgggcctgt ttattttgt 
      gtctcatat ggaagtacc aagaaaaaa ttgcactaa 
      tattaggct gcctttggg tagtacacg tccttgata 
      catattctc gaatctata gttcaaaaa ttattttac 
      caatttatc cagagttgt tctacaaca tgaaattta 
      tactatggg accatgctg gttcagtag ctaaagccc 
      ctattgcta atatgtcag gtctgatcg ctggtttag 
      gttttggct acacttttt acaaatgta gaaataaaa 
      aattattta ccaaaagga ttcatgtta ttttatggt 
      ggtttgcaa gtctcggag tttcaatat tgataatcc 
      cgattccag cactattta aaaaaacaa caatatttt 
      caaataacc ctgaagtta attttgcga tatatgctt 
      gtttaagca gaagtatct atatttttg ggacgggtt 
      tttttattt ttggaatga cacacggac aaggacata 
      taataagag caataaatg agtggcctc ctatgtacg 
      ttcttggcc gcctcactt acagtgctt accttttac 
      acgttccca tccagttgg tgaaagctg gcttgaaaa 
      gagcaccaa attatttga atgacttca ttttgaatt 
      gggagatgc tttcggatc ttttccttt cctacatgg 
      cggcgttgt tttttacac ttttctttg agctgccga 
      ttagtgagt taaaaaaac acattactt caatgttga 
      aaaatctaa ggagaaatg aggttactt gttacagca 
      ataaaacag aaaagtgtg aataaaggt tttgcagta 
      aactgtttt tttcttaat tatttttta caaaaacta 
      gttatatga aggtctctt aagtgttaa ccctaaaga 
      gactcagta gaaaccgcc attcaagtg aataaattt 
      atttgcaaa tagggtctc actctctct gaaacatta 
      agactctta gttactaac taaaaaata tagaaaagt 
      ggagaaatt aataaataa aaaaaatat actaaagtt 
      gattaagtg atactctaa atatttaac taaaacctt 
      gagagatag aaaacatta gaaattttg gaatctaaa 
      agctgaaga accgaaaca ctgagaaat ttattttta 
      aattgggag gtggaatct gtggcgact tgccgaata 
      aaattagaa ttaaaaatc aagtaatac acaacgtag 
      atatagtat atgtaaata cttaacaag ttatcaaga 
      aattaaaac aagacaaaa aagtttctt caatttttc 
      ttgtatttc gtaaataac gcatctaca aattggtga 
      ccccgacga acccaataa ccgaggatg tctaacgac 
      aacgtagga agtggagat acaacaata caccactac 
      cgacgccga cttcaacac tggtgagag tgatctcag 
      agactggcc atgtagcta tcgctgcac tttacttgc 
      ctcgatttt ggtcatcaa ggcctgact tatggtttg 
      cgatatcag agacaaaat tctgttaca aaaattctg 
      gaggtaggt ttagaagta gaagtcgta gtatatcta 
      gagaaccta accaaagca gaaataatc atcgaaaac 
      attattgtt ggtatcatt tcaaatttg atgctgaag 
      ctaaacgtt gcatacaac cgtatgaac tttaaaaac 
      acgccgatc aagggcggg agtaaccac agctctcgc 
      cccgctcaa aagtacgtg tcgcttatt tgtaaaaca 
      caaaaactc aaaaattag tttcttggt cgacacagg 
      tgcaaactt cagtatttt gcctgttaa ataccgaga 
      ttctaatcg taatcgaac atatgtacc tcgctttaa 
      tcttcaatc ggctagcgg ttttgtaac aaaagtgtt 
      cggagaaaa atcattaat ttaaattta ggacttgga 
      tgggtgtgg acgtacaca gaaaaagat tagtagact 
      cgacaagtt cattagaca tgtctgaaa aaagagact 
      cgtgacgta ctattcgcg cggtatttg aaaatacaa 
      attcagtat atca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY