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Gene information
General information
TC008518 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12078717..12091885
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008518
    iBeetle screen iB_07260

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008518 RA
      MEMEEEDTE KVKSFTVKA TDVPPEFFI TRDIAKRYF 
      RRFGKVKLV TFRPKRNTL LIEYSNEDG MLNALAVAG 
      EYNGHMFNV IRDTETIVK RKKVIKNPD PDWTDDPEV 
      KAELDAMGG FAPRTYDLR PEAMAVDVP ETLVTKTKR 
      QRWKGASPK PLKKKGGFS PEQSDLINL VRQQAVTIE 
      DKYRILDAR DKLIRIKIK KNLELTKSG ATVGTCPDM 
      CPEKERLMR ETQHQVALY EQEEGGKAM DPRLAVKQY 
      SRSSADQEA PLPQELRPV SVLQMTMGY LMHRIMGLC 
      DTPDVNLAE WYHFLWDRT RGIRKDITQ QELCCQGAV 
      ELVEQCARF HIFCSARLV AEDPSVFDQ KINTENLTK 
      CLQTLKYMY HDLALKGED CTNEAEFRA YIILLNLND 
      GNFMWEVQQ LKKHIQQSQ QVRFALEVY SSLDKQNYV 
      KFFKLINST TFLNACILM RYFIQVRLS AIKTLLKSY 
      SPRISQTSL PVSYLTKIL AFESQDSTI EFFETYGLF 
      TNIERTRIS LDRTNFGAP EFPYVLDRA INLVESKRI 
      FSVGRIVCG RELPPKLYE NHQPQNSFD QSGFLVFDF 
      EMDEIDSGK KQEIKMEEK IIEKIERVV ETPPPQIFT 
      PVTTVQSSQ NQTIFSQNK SSSVGIFAQ KTNEKSIFG 
      GKNIFGNVG NTTKSIFGG EITQLASPV AILPEVPKV 
      TPDEIEQKR IKEEEEEAK RKKDQELER IRKEEDEKR 
      RLKLLEDQE KKRRLDEAR KFEEMKRQM EEEAQRKQE 
      RRKNIEIKA TVSNILDEI LDTVEEKIK RETLAKIGR 
      KIRDRKLHH VMTKWREYA RRKRKRKAL DFDPLWFNQ 
      SIAEEACEL RTENQNLVL CNKRYKRGR VSDFVPKTD 
      DVGPIDLSE LTYKLLSRK HWDVEMKFR NELFWKVIV 
      SLPNDLSQV EKVLDKCIG WQHGVLIQQ SKSVPTVTY 
      CIEKQKGLA VNTRNDTNG FIFIANCFD DDLWRRIVE 
      NFKNFGVFV KIPVVLILQ NYDEAKPSL KTLIREGII 
      SDYLILVDK FTVRNLVNL IEEALIFLS TRVEKTPPL 
      QMDTLQSFL TKFLVTDLW KRCNSFSKW NSSYKNCLK 
      NPNTVIYLH NEALDRIKS IVLDEECRE YPKFPTSFR 
      DVLRSNIPD SLPCDYKYF PSFWDSDRY LAHLRSVLE 
      TLKLPNFLE PWPPCNQLN LELAVSKYC AQVVMEPQK 
      LFYKVMSVL LRFDFKDIA NVVWCDIIE LVVLEKLSQ 
      TDFSLYGTG FVNKSVYNQ LIVVYDVGK LDYLKSDWF 
      YVNNPIIKQ KIVEFLQHE EDTSKTEID VIDLDIDAI 
      FAKITQPQK QNVAKIKSE LKTCKKLLT DLEESVVIH 
      KSILQKSGN ILKSIIEEK
    CDS sequence
    • >TC008518 RA
      atggagatg gaagaggaa gacaccgaa aaagtgaag 
      tcttttacc gtaaaggcc accgatgtt ccccccgaa 
      tttttcata acgcgtgac attgccaaa cgttacttt 
      cgacgtttt gggaaagtt aaactcgtc acatttcgc 
      cccaaacgc aatacactt ctaattgaa tattcgaat 
      gaagatggg atgttaaac gctttagca gtcgcaggg 
      gaatacaac ggtcacatg tttaatgtg attcgagac 
      acggaaact attgttaaa agaaagaag gttataaaa 
      aatccagat ccagactgg acggatgat cccgaagtt 
      aaggccgaa ttggacgcc atggggggc ttcgcccct 
      cggacctac gatttgaga cccgaagcc atggctgtg 
      gatgttcct gaaacacta gtaacgaaa acaaaacgg 
      caaaggtgg aaaggagct tcacccaag ccacttaag 
      aagaagggg ggtttcagt ccggaacaa tccgattta 
      attaattta gttcgccaa caagctgtg acaattgag 
      gataaatat cgtattctc gatgctcgt gacaaatta 
      attcgcata aaaattaaa aaaaacctt gaattgact 
      aaaagtggg gcaactgtg ggcacatgc cctgacatg 
      tgccccgaa aaagagcga ctaatgcgc gaaacgcaa 
      caccaagtg gcgctctac gagcaggag gaagggggc 
      aaagcgatg gatccccgt cttgcggtt aaacaatac 
      tcacgaagc tcagccgat caggaagct cccttgcct 
      caggaatta cgaccggtt tcggtgctt caaatgaca 
      atggggtac cttatgcat cgaattatg ggcttatgt 
      gacacccct gatgtaaat ttagccgaa tggtatcat 
      ttcttatgg gaccgaact agagggatt cgtaaagat 
      atcactcaa caagagttg tgttgtcag ggggctgtt 
      gaattggta gaacagtgc gccagattc catattttt 
      tgttctgca agattggta gctgaagat ccgtctgtt 
      ttcgatcag aagattaat actgaaaat ttgacaaag 
      tgtctacaa acacttaaa tatatgtat catgatttg 
      gccttgaag ggtgaagac tgtacgaat gaggcggag 
      tttagagcc tatattatt ttgcttaat ttgaacgat 
      gggaatttt atgtgggag gtccaacaa ctcaaaaaa 
      cacatccaa caatcgcaa caagtccgc tttgccttg 
      gaagtatac tcatcattg gacaaacaa aactatgtg 
      aaatttttc aaattaatc aactcaaca acatttctc 
      aacgcttgt atcttaatg cgatatttt attcaagtg 
      cgattatcg gccataaaa actctccta aaatcatat 
      tcacctcga atatcacaa acatcactt cccgtctca 
      tacttgaca aaaatccta gctttcgaa agtcaagat 
      tcaacaatc gaatttttt gaaacttat ggcttattc 
      actaatatt gaacggaca agaattagt ttagatcga 
      acaaatttt ggtgcacca gaattcccc tatgtgcta 
      gaccgtgct ataaatcta gtggaatcg aaacgaatt 
      ttttcagtt ggtagaatt gtttgcggt agagaatta 
      ccaccgaaa ctttacgaa aatcaccaa ccacaaaat 
      agtttcgat caaagtggt tttttggtt tttgatttc 
      gagatggac gaaatcgat tcagggaaa aaacaagaa 
      atcaagatg gaggaaaaa ataattgaa aaaatagag 
      agagttgtt gagacgccg cctcctcaa atttttact 
      cctgtgacg acagttcag tcaagtcaa aatcaaacg 
      atcttcagt caaaataag tcaagtagt gttgggatt 
      ttcgcccaa aaaaccaac gaaaaaagt atttttggg 
      gggaaaaat atcttcggt aatgttggt aatactact 
      aaaagtata ttcggtggt gagataacc caactagcg 
      tcacctgtt gccatacta cctgaagta ccaaaagtc 
      acaccggac gaaatcgaa cagaaacga atcaaagaa 
      gaagaagaa gaagccaaa cgaaagaaa gatcaagaa 
      ttagaaaga ataaggaaa gaagaagac gaaaaacgc 
      cgtcttaaa ctcttagaa gatcaagaa aagaaacgc 
      cgacttgac gaagcccga aaattcgaa gaaatgaaa 
      cgtcaaatg gaggaagaa gcacaacgg aagcaagaa 
      cgtcgcaaa aacatcgaa attaaagca accgtaagc 
      aacatcctt gatgagatt ttagacacg gttgaagaa 
      aaaattaag cgtgagacc ctggcgaaa atcgggcgg 
      aaaatccgc gaccggaaa ttacaccac gtgatgaca 
      aaatggcgc gaatacgct cgccggaaa cggaaacgt 
      aaagctctc gattttgat ccattatgg ttcaatcaa 
      tcaattgcc gaagaagct tgtgaatta cgaacagaa 
      aaccaaaac ttggttttg tgtaataag agatacaaa 
      cgtgggcgg gttagtgat tttgtacct aaaaccgac 
      gatgttggt ccgattgac ttgtcagaa ttgacgtat 
      aagttacta agtcgaaaa cattgggat gttgaaatg 
      aagtttcgt aacgaattg ttctggaaa gtaattgtt 
      tcgttgccg aacgatttg agtcaagtt gagaaggtt 
      ttggataaa tgtataggt tggcaacat ggtgtttta 
      atccaacag tcgaagtca gtgccaact gtgacgtac 
      tgtatcgag aaacagaaa ggtctagcc gtaaacaca 
      cgaaacgac acgaatgga tttatcttt attgcgaat 
      tgttttgac gatgatctt tggcgacgt attgttgaa 
      aattttaaa aatttcggc gtttttgtt aaaattcca 
      gtcgtttta atattacag aactatgat gaggcaaag 
      ccgagtctt aaaacctta attagggaa ggaataatt 
      tccgattat ttgatactt gtggataag ttcacggtc 
      cgaaatttg gtgaatcta atagaggag gctttgatt 
      tttctttcg acacgagtc gagaaaaca ccaccgttg 
      caaatggac actttacaa tcgtttcta acgaagttt 
      ttggtcacg gatttgtgg aaacgttgc aatagtttc 
      tcaaagtgg aattcgagt tataaaaat tgtttaaaa 
      aatccgaac actgtgatt tatctccac aacgaagcc 
      ttggaccga atcaaaagc attgttttg gatgaagag 
      tgtcgagaa tacccgaag tttccgacc agctttcgc 
      gatgtgctt cgtagtaat atccccgat tcgcttcct 
      tgcgactat aaatatttc cccagtttt tgggatagt 
      gacagatat ttagcgcat ctgaggtcg gttttagag 
      actttaaaa ctgcccaat tttctcgaa ccttggcca 
      ccctgcaac caactcaac ctcgaatta gctgtctca 
      aaatattgc gctcaggtg gtaatggag ccacaaaaa 
      ctattctac aaagtgatg agtgtctta ttgcggttt 
      gattttaaa gatattgcg aatgtcgtc tggtgtgat 
      attatcgag ttggtagtg ctggaaaag ctaagccag 
      actgatttc tccttgtat ggaactggt tttgtcaat 
      aagtcggtt tataaccag ttgattgtg gtctacgat 
      gttggtaaa cttgattat ttgaaaagt gattggttt 
      tatgttaat aatccgatt attaagcaa aaaattgtc 
      gaattttta caacacgag gaagatacc agtaaaacg 
      gaaatcgat gttatcgac ttggatatt gatgcgatt 
      tttgccaaa ataacgcaa ccgcaaaaa caaaatgtt 
      gctaaaatt aagagcgag ttgaaaact tgtaaaaaa 
      ttgttgact gatttggag gagagtgtt gtcatacat 
      aaaagtatt ttgcaaaag tcggggaat attttaaaa 
      agtattata gaggaaaag tga
    mRNA sequence
    • >TC008518 RA
      gggttggtg acattgcca aagatggcg cggttgtgt 
      aagttttga aatgagtta atttactca aaaatctgt 
      gttagtgtg tgtaaaaat tgcccaaaa ttagtatca 
      ataacagcc gcagcatgg agatggaag aggaagaca 
      ccgaaaaag tgaagtctt ttaccgtaa aggccaccg 
      atgttcccc ccgaatttt tcataacgc gtgacattg 
      ccaaacgtt actttcgac gttttggga aagttaaac 
      tcgtcacat ttcgcccca aacgcaata cacttctaa 
      ttgaatatt cgaatgaag atgggatgt taaacgctt 
      tagcagtcg caggggaat acaacggtc acatgttta 
      atgtgattc gagacacgg aaactattg ttaaaagaa 
      agaaggtta taaaaaatc cagatccag actggacgg 
      atgatcccg aagttaagg ccgaattgg acgccatgg 
      ggggcttcg cccctcgga cctacgatt tgagacccg 
      aagccatgg ctgtggatg ttcctgaaa cactagtaa 
      cgaaaacaa aacggcaaa ggtggaaag gagcttcac 
      ccaagccac ttaagaaga aggggggtt tcagtccgg 
      aacaatccg atttaatta atttagttc gccaacaag 
      ctgtgacaa ttgaggata aatatcgta ttctcgatg 
      ctcgtgaca aattaattc gcataaaaa ttaaaaaaa 
      accttgaat tgactaaaa gtggggcaa ctgtgggca 
      catgccctg acatgtgcc ccgaaaaag agcgactaa 
      tgcgcgaaa cgcaacacc aagtggcgc tctacgagc 
      aggaggaag ggggcaaag cgatggatc cccgtcttg 
      cggttaaac aatactcac gaagctcag ccgatcagg 
      aagctccct tgcctcagg aattacgac cggtttcgg 
      tgcttcaaa tgacaatgg ggtacctta tgcatcgaa 
      ttatgggct tatgtgaca cccctgatg taaatttag 
      ccgaatggt atcatttct tatgggacc gaactagag 
      ggattcgta aagatatca ctcaacaag agttgtgtt 
      gtcaggggg ctgttgaat tggtagaac agtgcgcca 
      gattccata ttttttgtt ctgcaagat tggtagctg 
      aagatccgt ctgttttcg atcagaaga ttaatactg 
      aaaatttga caaagtgtc tacaaacac ttaaatata 
      tgtatcatg atttggcct tgaagggtg aagactgta 
      cgaatgagg cggagttta gagcctata ttattttgc 
      ttaatttga acgatggga attttatgt gggaggtcc 
      aacaactca aaaaacaca tccaacaat cgcaacaag 
      tccgctttg ccttggaag tatactcat cattggaca 
      aacaaaact atgtgaaat ttttcaaat taatcaact 
      caacaacat ttctcaacg cttgtatct taatgcgat 
      attttattc aagtgcgat tatcggcca taaaaactc 
      tcctaaaat catattcac ctcgaatat cacaaacat 
      cacttcccg tctcatact tgacaaaaa tcctagctt 
      tcgaaagtc aagattcaa caatcgaat tttttgaaa 
      cttatggct tattcacta atattgaac ggacaagaa 
      ttagtttag atcgaacaa attttggtg caccagaat 
      tcccctatg tgctagacc gtgctataa atctagtgg 
      aatcgaaac gaatttttt cagttggta gaattgttt 
      gcggtagag aattaccac cgaaacttt acgaaaatc 
      accaaccac aaaatagtt tcgatcaaa gtggttttt 
      tggtttttg atttcgaga tggacgaaa tcgattcag 
      ggaaaaaac aagaaatca agatggagg aaaaaataa 
      ttgaaaaaa tagagagag ttgttgaga cgccgcctc 
      ctcaaattt ttactcctg tgacgacag ttcagtcaa 
      gtcaaaatc aaacgatct tcagtcaaa ataagtcaa 
      gtagtgttg ggattttcg cccaaaaaa ccaacgaaa 
      aaagtattt ttgggggga aaaatatct tcggtaatg 
      ttggtaata ctactaaaa gtatattcg gtggtgaga 
      taacccaac tagcgtcac ctgttgcca tactacctg 
      aagtaccaa aagtcacac cggacgaaa tcgaacaga 
      aacgaatca aagaagaag aagaagaag ccaaacgaa 
      agaaagatc aagaattag aaagaataa ggaaagaag 
      aagacgaaa aacgccgtc ttaaactct tagaagatc 
      aagaaaaga aacgccgac ttgacgaag cccgaaaat 
      tcgaagaaa tgaaacgtc aaatggagg aagaagcac 
      aacggaagc aagaacgtc gcaaaaaca tcgaaatta 
      aagcaaccg taagcaaca tccttgatg agattttag 
      acacggttg aagaaaaaa ttaagcgtg agaccctgg 
      cgaaaatcg ggcggaaaa tccgcgacc ggaaattac 
      accacgtga tgacaaaat ggcgcgaat acgctcgcc 
      ggaaacgga aacgtaaag ctctcgatt ttgatccat 
      tatggttca atcaatcaa ttgccgaag aagcttgtg 
      aattacgaa cagaaaacc aaaacttgg ttttgtgta 
      ataagagat acaaacgtg ggcgggtta gtgattttg 
      tacctaaaa ccgacgatg ttggtccga ttgacttgt 
      cagaattga cgtataagt tactaagtc gaaaacatt 
      gggatgttg aaatgaagt ttcgtaacg aattgttct 
      ggaaagtaa ttgtttcgt tgccgaacg atttgagtc 
      aagttgaga aggttttgg ataaatgta taggttggc 
      aacatggtg ttttaatcc aacagtcga agtcagtgc 
      caactgtga cgtactgta tcgagaaac agaaaggtc 
      tagccgtaa acacacgaa acgacacga atggattta 
      tctttattg cgaattgtt ttgacgatg atctttggc 
      gacgtattg ttgaaaatt ttaaaaatt tcggcgttt 
      ttgttaaaa ttccagtcg ttttaatat tacagaact 
      atgatgagg caaagccga gtcttaaaa ccttaatta 
      gggaaggaa taatttccg attatttga tacttgtgg 
      ataagttca cggtccgaa atttggtga atctaatag 
      aggaggctt tgatttttc tttcgacac gagtcgaga 
      aaacaccac cgttgcaaa tggacactt tacaatcgt 
      ttctaacga agtttttgg tcacggatt tgtggaaac 
      gttgcaata gtttctcaa agtggaatt cgagttata 
      aaaattgtt taaaaaatc cgaacactg tgatttatc 
      tccacaacg aagccttgg accgaatca aaagcattg 
      ttttggatg aagagtgtc gagaatacc cgaagtttc 
      cgaccagct ttcgcgatg tgcttcgta gtaatatcc 
      ccgattcgc ttccttgcg actataaat atttcccca 
      gtttttggg atagtgaca gatatttag cgcatctga 
      ggtcggttt tagagactt taaaactgc ccaattttc 
      tcgaacctt ggccaccct gcaaccaac tcaacctcg 
      aattagctg tctcaaaat attgcgctc aggtggtaa 
      tggagccac aaaaactat tctacaaag tgatgagtg 
      tcttattgc ggtttgatt ttaaagata ttgcgaatg 
      tcgtctggt gtgatatta tcgagttgg tagtgctgg 
      aaaagctaa gccagactg atttctcct tgtatggaa 
      ctggttttg tcaataagt cggtttata accagttga 
      ttgtggtct acgatgttg gtaaacttg attatttga 
      aaagtgatt ggttttatg ttaataatc cgattatta 
      agcaaaaaa ttgtcgaat ttttacaac acgaggaag 
      ataccagta aaacggaaa tcgatgtta tcgacttgg 
      atattgatg cgatttttg ccaaaataa cgcaaccgc 
      aaaaacaaa atgttgcta aaattaaga gcgagttga 
      aaacttgta aaaaattgt tgactgatt tggaggaga 
      gtgttgtca tacataaaa gtattttgc aaaagtcgg 
      ggaatattt taaaaagta ttatagagg aaaagtgat 
      gtaataaaa tgactcatg aaaaagata taatagtat 
      acagtggaa cctctacaa tccgaagta gtagaagaa 
      gatggacaa ctgaaataa tcaataatg cgccctttg 
      gtggttaat tttgaaatc gcttcttta caaaaaagc 
      ggtaatgac aaccgacat ttttgttta ctaattttg 
      atagtaccc ccacccccc tttatcatc ctggtaata 
      ataaaaaaa taaaaactc gattatttc acccaaaag 
      tctttatct ttttgctgt tacttattt ataaggggc 
      gtttgtttt tttcattta aacattttg agggaaaac 
      acttattgc aaaacctaa atacaaaaa aataataaa 
      aaaatcaat cgttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY