Status
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Gene information
General information
TC032955
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007013
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11927842..11937054
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032955
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07259

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032955 RA
      MNGKTPISL LQELMAARG ISLPVYIEE GFGTSFKCT 
      VKAGGVTAC GYGTSKKNA KHESAKNAL VKLRREDKS 
      PEIKPRAEN NNLFRNFVG ELNEYAASR YGAKYPSYD 
      FLTVSVDGE FFFKCSFAN EESIGEGMN KKDAKQDSA 
      RKMLELVKK NQPVYERKV QEKTTITIN NVEEIIEKY 
      KQHSLSETS MVNSSKAGQ VFEPTPGII LADEESIDS 
      LRNKLKSHN ICYTLTQFQ INPCIMYVQ INGTDLCFM 
      AVGDDREEA TRNVLRQVL QVKKTGCSL STNHLLNFN
    CDS sequence
    • >TC032955 RA
      atgaacgga aaaacaccg atttctctc ttacaagag 
      ttaatggct gctcgggga atttccctt ccggtctac 
      atcgaagaa ggcttcggc acttcattc aaatgcact 
      gttaaagca ggtggtgta acagcttgt ggttacgga 
      acgtctaaa aaaaacgca aaacacgaa agcgccaaa 
      aacgccctt gtaaaatta agacgagag gacaagtct 
      ccagaaatt aaaccacga gctgaaaat aataattta 
      tttcgaaat tttgtgggc gaattgaat gaatatgca 
      gcatccaga tatggcgca aagtatcca agttatgat 
      tttttaacc gtttcggtg gacggtgag ttttttttc 
      aagtgttct tttgcgaat gaggaaagt attggagaa 
      gggatgaac aaaaaagat gccaaacaa gattctgca 
      agaaaaatg ttggaattg gttaagaaa aatcaacca 
      gtttatgag cgaaaggta caagaaaaa acaacaatt 
      acaataaac aatgttgaa gaaattata gaaaagtat 
      aaacaacat tcattgagc gaaaccagc atggttaat 
      tcatcaaaa gctgggcaa gtttttgaa cctactcct 
      ggtatcatc ttggccgat gaagaaagc atagatagt 
      cttcgaaat aaacttaaa tcgcacaac atttgctac 
      acattaacg caattccag ataaaccct tgcattatg 
      tatgtgcaa ataaatgga actgatttg tgttttatg 
      gctgttgga gatgatcga gaggaagcg actagaaat 
      gttcttcgt caggtgctt caagttaag aaaactgga 
      tgttctctt agtactaat cacctgttg aattttaat 
      tga
    mRNA sequence
    • >TC032955 RA
      tctgtctag cttgaaacc taacctctc tctttttct 
      atgttccac tacgaagtc gtgtaaaac cccgatgac 
      agtttttca taatgacag tgcctgaga ctgctttga 
      cctggtttt gttgatggg tctaaaatg aacggaaaa 
      acaccgatt tctctctta caagagtta atggctgct 
      cggggaatt tcccttccg gtctacatc gaagaaggc 
      ttcggcact tcattcaaa tgcactgtt aaagcaggt 
      ggtgtaaca gcttgtggt tacggaacg tctaaaaaa 
      aacgcaaaa cacgaaagc gccaaaaac gcccttgta 
      aaattaaga cgagaggac aagtctcca gaaattaaa 
      ccacgagct gaaaataat aatttattt cgaaatttt 
      gtgggcgaa ttgaatgaa tatgcagca tccagatat 
      ggcgcaaag tatccaagt tatgatttt ttaaccgtt 
      tcggtggac ggtgagttt tttttcaag tgttctttt 
      gcgaatgag gaaagtatt ggagaaggg atgaacaaa 
      aaagatgcc aaacaagat tctgcaaga aaaatgttg 
      gaattggtt aagaaaaat caaccagtt tatgagcga 
      aaggtacaa gaaaaaaca acaattaca ataaacaat 
      gttgaagaa attatagaa aagtataaa caacattca 
      ttgagcgaa accagcatg gttaattca tcaaaagct 
      gggcaagtt tttgaacct actcctggt atcatcttg 
      gccgatgaa gaaagcata gatagtctt cgaaataaa 
      cttaaatcg cacaacatt tgctacaca ttaacgcaa 
      ttccagata aacccttgc attatgtat gtgcaaata 
      aatggaact gatttgtgt tttatggct gttggagat 
      gatcgagag gaagcgact agaaatgtt cttcgtcag 
      gtgcttcaa gttaagaaa actggatgt tctcttagt 
      actaatcac ctgttgaat tttaattga tttttatta 
      ttatttatt tgcctaggt taagtataa ttatgtgcc 
      tgcaattgt tgttggaga tcaattttt tttaaacat 
      ttttacact cgtatcgat attttcaaa taaagatag 
      gagaaaatt aacagtttg acaatagta aaaactatt 
      gaacaaatt ttaaatact ttactcagt atcagtcta 
      gatcaagta ataacatta agctaccga tttgtttca 
      tttttatct aaacttttt ttgaaggaa attttattc 
      tctacaact ttgtttctt gggggctga tttaaagtt 
      tggtcgcaa agttgcatg ggtctctgt tgcttagtt 
      gcaacaaat agcattgca aaatgcaag atttgcgcc 
      gttgctata acaataccg atttgcgcc gttgctata 
      acaataccg atttgcgcc ggtcataag tcaacttga 
      aatcagccc ctatgtctt ttttggtat cacaaccca 
      tttaaaatt atagaacgg tgcgcaaat tagtaaaag 
      tttgactga aatatagtt tttgatatt tttttccta 
      atcccgacc agatgttag atttaatgt ttaattttg 
      gtgtgatgt attgttact tattaccta attaataat 
      gaaatgaaa ttcattacg taactagtt ttgttaact 
      cttaaaaaa attgttgga ataaatttt ccattaggt 
      attaggaaa aattgtgta cgtaatacg ttatgaaag 
      gcctattat tgtttaact cgcataatt tgcaaaact 
      tgtttgctg cgctatgtc attttggcg tatattttg 
      ctctttaaa caaaatcgg tgttttcat aattaatca 
      cgtaaacta ttcttttaa aacactata cgtacaaaa 
      aaatatctt tgttatcag gggcggatc cagaaatta 
      attttagag ggactttct aaaattttt aaaaggagt 
      caaaattct tacggcctg atttcaagt ttggtcgca 
      aagttgcgt gggtcgcag ttgcatagc agcaacgaa 
      tgacattgc taggtttgc accgttact ataacaata 
      cggacttgc gtcggttgt aactcaact tgaaatcag 
      cccattagt gaacagtca tttaactaa aagacaaaa 
      atattcaaa taacaaaaa atctgaatc cgtccttgt 
      ttgttattt gtattttgt tttatttta ttattgttg 
      taaaatatt tatttttaa acaaatttt aaatcgcaa 
      ttacaaatt tgttttttt tgttggtaa cattttaaa 
      gccttttta atatctaaa tcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY