Status
Please wait ...
Gene information
General information
TC007042 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10905765..10911373
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007042
    iBeetle screen iB_07255

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007042 RA
      MTGHRDLPV FVTVKRESV VFIVLCYYL LFFAFTGAY 
      GEDVKEKKK PETVQDLFN TSSCIPKPY RCPHRQIQF 
      FLYTRRTQK TPELLDTTK EESLYNSQF NRINPTKIV 
      IHGFGGGRN LSPSTDMRE AYFHRGNYN IIIVDYGTL 
      VREPCLKQM EWAPRFCAK CIAQLINYL THHPRGVRA 
      DDLHLVGYS VGAHIAGLV ANYLNPIEH GKLGRITGL 
      DPTIVFYMG NNRSRDLDY TDAHFVDVL HTGAGILGQ 
      WGPTGHADF YINGGSSQP GCGKDTIFK TLACDHTKV 
      TPYFIESIV TKKGFWAYP CPTLLSFMT GFCSPKDEE 
      YVLMGEHVT HKARGIYYV STNPNPPYA QGPPKKQIG 
      RRGLLNRLF
    CDS sequence
    • >TC007042 RA
      atgactggt catcgagac ttgccggta tttgtgaca 
      gtgaaacga gaaagtgtg gtgtttatt gttctttgc 
      tattatctc ctcttcttc gcgtttacc ggcgcttac 
      ggcgaagac gtcaaggaa aagaaaaag ccagagacg 
      gttcaagac ctttttaac acctcctct tgcatccct 
      aagccttat aggtgtcct caccgccaa atccaattt 
      ttcttatac acaaggcgg acccagaaa actccggag 
      ctgttagac acaaccaaa gaagagtcg ttgtacaat 
      agtcaattt aatagaatt aatccaacg aaaattgtg 
      atccatggg tttggtggg gggcgcaat ttatcgccg 
      agtactgac atgagagaa gcctatttc cacagaggg 
      aactacaac attatcatt gtggattac ggcacttta 
      gtccgggaa ccatgcttg aagcaaatg gaatgggcg 
      ccccgtttc tgcgccaaa tgcattgcc cagctcatc 
      aattaccta acccatcat cccagaggc gtaagagcc 
      gatgacttg catttggtt ggttatagt gttggtgct 
      cacattgct ggtctcgtt gcgaattat ctcaatccc 
      atcgaacac ggaaaactg ggtcgaatt accggcctg 
      gaccccacc atcgtcttc tacatgggc aacaacaga 
      tcgcgagac ctggattat actgatgcc catttcgtt 
      gatgttttg cacacaggg gccgggatt ttggggcaa 
      tgggggccc acaggacac gccgatttt tacatcaat 
      ggggggtca agtcagccg gggtgtggc aaagacacc 
      atattcaaa actttggcg tgtgatcac acgaaagtg 
      acaccgtat tttatcgag tctattgtg acgaaaaaa 
      gggttctgg gcatatcct tgcccaacg ttgttaagt 
      ttcatgacg ggtttttgt agccctaag gacgaggag 
      tacgttttg atgggcgaa catgtgaca cataaggct 
      cgtggtatt tactacgtt agcactaat ccgaaccca 
      ccttatgca caaggacct ccgaagaaa caaattggg 
      aggagaggt ctcctcaac cgactgttt taa
    mRNA sequence
    • >TC007042 RA
      gtgttgttg ttactcaac atccgacga cacattttc 
      aaaaaaatg tgcccggaa gcatgggat aattgatgg 
      tactgtaat gggtgccaa aacattagc acctgaatt 
      ttctccctc ttacgcaac tcagtgtta tcatcaaaa 
      atccccatg tgtggctca tatcccggt gagagtggt 
      tggaggagg ttgtaaaag tgaaggtac tcttcgtac 
      gacgatgca tacggcgat tgaccatcg tgcaggtcg 
      cttgatcga ctttcactg ctaaaaatt actcaaata 
      gactaaaac agggccaat gttaggatg actggtcat 
      cgagacttg ccggtattt gtgacagtg aaacgagaa 
      agtgtggtg tttattgtt ctttgctat tatctcctc 
      ttcttcgcg tttaccggc gcttacggc gaagacgtc 
      aaggaaaag aaaaagcca gagacggtt caagacctt 
      tttaacacc tcctcttgc atccctaag ccttatagg 
      tgtcctcac cgccaaatc caatttttc ttatacaca 
      aggcggacc cagaaaact ccggagctg ttagacaca 
      accaaagaa gagtcgttg tacaatagt caatttaat 
      agaattaat ccaacgaaa attgtgatc catgggttt 
      ggtgggggg cgcaattta tcgccgagt actgacatg 
      agagaagcc tatttccac agagggaac tacaacatt 
      atcattgtg gattacggc actttagtc cgggaacca 
      tgcttgaag caaatggaa tgggcgccc cgtttctgc 
      gccaaatgc attgcccag ctcatcaat tacctaacc 
      catcatccc agaggcgta agagccgat gacttgcat 
      ttggttggt tatagtgtt ggtgctcac attgctggt 
      ctcgttgcg aattatctc aatcccatc gaacacgga 
      aaactgggt cgaattacc ggcctggac cccaccatc 
      gtcttctac atgggcaac aacagatcg cgagacctg 
      gattatact gatgcccat ttcgttgat gttttgcac 
      acaggggcc gggattttg gggcaatgg gggcccaca 
      ggacacgcc gatttttac atcaatggg gggtcaagt 
      cagccgggg tgtggcaaa gacaccata ttcaaaact 
      ttggcgtgt gatcacacg aaagtgaca ccgtatttt 
      atcgagtct attgtgacg aaaaaaggg ttctgggca 
      tatccttgc ccaacgttg ttaagtttc atgacgggt 
      ttttgtagc cctaaggac gaggagtac gttttgatg 
      ggcgaacat gtgacacat aaggctcgt ggtatttac 
      tacgttagc actaatccg aacccacct tatgcacaa 
      ggacctccg aagaaacaa attgggagg agaggtctc 
      ctcaaccga ctgttttaa gtcaaaaat gccaaaatt 
      tggactacg gtttgacac aattagcca gtagttttt 
      tgtaattaa aagtttgcc attaggact aataattta 
      ttgtagttc ttagatgtt ttgttaata aaatttgag 
      taaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY