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Gene information
General information
TC011005 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6863403..6865279
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011005
    iBeetle screen iB_07251

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011005 RA
      MVSTRGIKL KFCENERVL CYEPDPTKA KVLYDSKVL 
DVVVAKDQR GRKTVEYLI HFQGWNSSW DRYVSEEYV 
LKDTPENRQ LQKDLAEKS QLQLGAYLY RRDRKKSRK 
TSTTAPSSD DCSSGSPTR MDTDDGQGV TSSSEDDSS 
IEEETVPIE LTPELRACL EQDYCLINT KNKLVKLPA 
EPNVVTILE NYWRHYANG QISDLNEKT SQRHRYPFN 
NTQRRRPED VQRNLNICS EVLDGIRLY FDYTVNDLL 
LYKCEQGQI ETKQAVYTS IHLDKEVSS SKNEVSYMD 
YITYPNNHD DYQNGNRQA MQRKRSLRS NKSTDAVSN 
GNSQDSAKA KASSSVDNN DSCGSRPLS LRLLPEHVY 
NQQPPPPCL VYGAIHLTR LFVKLPELL NAATIDEKK 
WTTLLQHMD TFIDYLNEH REWFGEKFY IDRQ
    CDS sequence
    • >TC011005 RA
      atggtttca actcgaggt atcaaatta aagttctgt 
gaaaacgag cgcgtgttg tgttatgag cctgacccg 
accaaagcc aaagtcttg tacgactcc aaagtgttg 
gacgtagta gtggccaag gaccaacgt ggacgcaaa 
accgtcgag tacctcatt cattttcag ggctggaat 
tcctcatgg gacagatac gtcagtgag gagtacgtt 
ttgaaagac actcccgaa aacaggcaa ttgcagaaa 
gacttggcc gaaaaatcc caactgcaa ttaggggcg 
tatttgtac agacgggac cggaaaaag agtcgcaag 
actagcaca acagccccc tcttctgat gactgcagt 
tccgggagc cccacgagg atggacacg gacgatggg 
caaggcgtg acaagcagt tctgaagat gattcttca 
attgaggaa gaaacggtg ccgattgag ctaacgccc 
gaattacgg gcgtgtctc gaacaggac tattgtctt 
atcaatacg aaaaataaa ctagttaaa ttaccggcc 
gaaccaaat gttgttaca attttggaa aattattgg 
aggcattat gccaatggc cagattagt gatttgaac 
gaaaagacc agtcagagg cacaggtat ccgttcaat 
aacacgcaa aggaggcgg cctgaagat gtacaaaga 
aatttaaat atttgtagc gaggttctg gacggcatc 
cgtctgtat tttgattac acggtcaat gatttgttg 
ctgtataag tgtgaacaa gggcaaatt gagacgaag 
caggctgtg tatacttct attcatttg gacaaagaa 
gtctcaagt tcaaaaaat gaggttagt tatatggat 
tacataacg tatccaaac aatcatgat gattaccaa 
aatggcaat aggcaagcc atgcaaagg aagcgaagt 
ctgagatca aacaaaagc actgacgct gtttcgaat 
ggtaatagt caggatagc gctaaagca aaagctagt 
tcaagcgtt gacaataat gacagttgt ggctcacgc 
ccactttcg ctccgcctt ttacctgaa catgtgtac 
aatcaacaa ccccctccc ccatgtctt gtgtacggt 
gccattcat ttgacgcga ctttttgtc aaattgccc 
gaattgttg aacgcagcc accattgat gagaaaaaa 
tggacaact ttattgcaa cacatggac acttttatt 
gattactta aacgagcat cgggagtgg tttggggaa 
aagttctac atcgacagg caataa
    mRNA sequence
    • >TC011005 RA
      catgtcaac aacaaagtg aatttaaac caaatataa 
acaatacga taatatggt ttcaactcg aggtatcaa 
attaaagtt ctgtgaaaa cgagcgcgt gttgtgtta 
tgagcctga cccgaccaa agccaaagt cttgtacga 
ctccaaagt gttggacgt agtagtggc caaggacca 
acgtggacg caaaaccgt cgagtacct cattcattt 
tcagggctg gaattcctc atgggacag atacgtcag 
tgaggagta cgttttgaa agacactcc cgaaaacag 
gcaattgca gaaagactt ggccgaaaa atcccaact 
gcaattagg ggcgtattt gtacagacg ggaccggaa 
aaagagtcg caagactag cacaacagc cccctcttc 
tgatgactg cagttccgg gagccccac gaggatgga 
cacggacga tgggcaagg cgtgacaag cagttctga 
agatgattc ttcaattga ggaagaaac ggtgccgat 
tgagctaac gcccgaatt acgggcgtg tctcgaaca 
ggactattg tcttatcaa tacgaaaaa taaactagt 
taaattacc ggccgaacc aaatgttgt tacaatttt 
ggaaaatta ttggaggca ttatgccaa tggccagat 
tagtgattt gaacgaaaa gaccagtca gaggcacag 
gtatccgtt caataacac gcaaaggag gcggcctga 
agatgtaca aagaaattt aaatatttg tagcgaggt 
tctggacgg catccgtct gtattttga ttacacggt 
caatgattt gttgctgta taagtgtga acaagggca 
aattgagac gaagcaggc tgtgtatac ttctattca 
tttggacaa agaagtctc aagttcaaa aaatgaggt 
tagttatat ggattacat aacgtatcc aaacaatca 
tgatgatta ccaaaatgg caataggca agccatgca 
aaggaagcg aagtctgag atcaaacaa aagcactga 
cgctgtttc gaatggtaa tagtcagga tagcgctaa 
agcaaaagc tagttcaag cgttgacaa taatgacag 
ttgtggctc acgcccact ttcgctccg ccttttacc 
tgaacatgt gtacaatca acaaccccc tcccccatg 
tcttgtgta cggtgccat tcatttgac gcgactttt 
tgtcaaatt gcccgaatt gttgaacgc agccaccat 
tgatgagaa aaaatggac aactttatt gcaacacat 
ggacacttt tattgatta cttaaacga gcatcggga 
gtggtttgg ggaaaagtt ctacatcga caggcaata 
aaacaaggc gtttttaat agaagatgt attacctac 
aagtaaata tttggtaac aaataaata tgtcattat 
tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY