Status
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Gene information
General information
TC011005 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6863403..6865279
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011005
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07251

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011005 RA
      MVSTRGIKL KFCENERVL CYEPDPTKA KVLYDSKVL 
      DVVVAKDQR GRKTVEYLI HFQGWNSSW DRYVSEEYV 
      LKDTPENRQ LQKDLAEKS QLQLGAYLY RRDRKKSRK 
      TSTTAPSSD DCSSGSPTR MDTDDGQGV TSSSEDDSS 
      IEEETVPIE LTPELRACL EQDYCLINT KNKLVKLPA 
      EPNVVTILE NYWRHYANG QISDLNEKT SQRHRYPFN 
      NTQRRRPED VQRNLNICS EVLDGIRLY FDYTVNDLL 
      LYKCEQGQI ETKQAVYTS IHLDKEVSS SKNEVSYMD 
      YITYPNNHD DYQNGNRQA MQRKRSLRS NKSTDAVSN 
      GNSQDSAKA KASSSVDNN DSCGSRPLS LRLLPEHVY 
      NQQPPPPCL VYGAIHLTR LFVKLPELL NAATIDEKK 
      WTTLLQHMD TFIDYLNEH REWFGEKFY IDRQ
    CDS sequence
    • >TC011005 RA
      atggtttca actcgaggt atcaaatta aagttctgt 
      gaaaacgag cgcgtgttg tgttatgag cctgacccg 
      accaaagcc aaagtcttg tacgactcc aaagtgttg 
      gacgtagta gtggccaag gaccaacgt ggacgcaaa 
      accgtcgag tacctcatt cattttcag ggctggaat 
      tcctcatgg gacagatac gtcagtgag gagtacgtt 
      ttgaaagac actcccgaa aacaggcaa ttgcagaaa 
      gacttggcc gaaaaatcc caactgcaa ttaggggcg 
      tatttgtac agacgggac cggaaaaag agtcgcaag 
      actagcaca acagccccc tcttctgat gactgcagt 
      tccgggagc cccacgagg atggacacg gacgatggg 
      caaggcgtg acaagcagt tctgaagat gattcttca 
      attgaggaa gaaacggtg ccgattgag ctaacgccc 
      gaattacgg gcgtgtctc gaacaggac tattgtctt 
      atcaatacg aaaaataaa ctagttaaa ttaccggcc 
      gaaccaaat gttgttaca attttggaa aattattgg 
      aggcattat gccaatggc cagattagt gatttgaac 
      gaaaagacc agtcagagg cacaggtat ccgttcaat 
      aacacgcaa aggaggcgg cctgaagat gtacaaaga 
      aatttaaat atttgtagc gaggttctg gacggcatc 
      cgtctgtat tttgattac acggtcaat gatttgttg 
      ctgtataag tgtgaacaa gggcaaatt gagacgaag 
      caggctgtg tatacttct attcatttg gacaaagaa 
      gtctcaagt tcaaaaaat gaggttagt tatatggat 
      tacataacg tatccaaac aatcatgat gattaccaa 
      aatggcaat aggcaagcc atgcaaagg aagcgaagt 
      ctgagatca aacaaaagc actgacgct gtttcgaat 
      ggtaatagt caggatagc gctaaagca aaagctagt 
      tcaagcgtt gacaataat gacagttgt ggctcacgc 
      ccactttcg ctccgcctt ttacctgaa catgtgtac 
      aatcaacaa ccccctccc ccatgtctt gtgtacggt 
      gccattcat ttgacgcga ctttttgtc aaattgccc 
      gaattgttg aacgcagcc accattgat gagaaaaaa 
      tggacaact ttattgcaa cacatggac acttttatt 
      gattactta aacgagcat cgggagtgg tttggggaa 
      aagttctac atcgacagg caataa
    mRNA sequence
    • >TC011005 RA
      catgtcaac aacaaagtg aatttaaac caaatataa 
      acaatacga taatatggt ttcaactcg aggtatcaa 
      attaaagtt ctgtgaaaa cgagcgcgt gttgtgtta 
      tgagcctga cccgaccaa agccaaagt cttgtacga 
      ctccaaagt gttggacgt agtagtggc caaggacca 
      acgtggacg caaaaccgt cgagtacct cattcattt 
      tcagggctg gaattcctc atgggacag atacgtcag 
      tgaggagta cgttttgaa agacactcc cgaaaacag 
      gcaattgca gaaagactt ggccgaaaa atcccaact 
      gcaattagg ggcgtattt gtacagacg ggaccggaa 
      aaagagtcg caagactag cacaacagc cccctcttc 
      tgatgactg cagttccgg gagccccac gaggatgga 
      cacggacga tgggcaagg cgtgacaag cagttctga 
      agatgattc ttcaattga ggaagaaac ggtgccgat 
      tgagctaac gcccgaatt acgggcgtg tctcgaaca 
      ggactattg tcttatcaa tacgaaaaa taaactagt 
      taaattacc ggccgaacc aaatgttgt tacaatttt 
      ggaaaatta ttggaggca ttatgccaa tggccagat 
      tagtgattt gaacgaaaa gaccagtca gaggcacag 
      gtatccgtt caataacac gcaaaggag gcggcctga 
      agatgtaca aagaaattt aaatatttg tagcgaggt 
      tctggacgg catccgtct gtattttga ttacacggt 
      caatgattt gttgctgta taagtgtga acaagggca 
      aattgagac gaagcaggc tgtgtatac ttctattca 
      tttggacaa agaagtctc aagttcaaa aaatgaggt 
      tagttatat ggattacat aacgtatcc aaacaatca 
      tgatgatta ccaaaatgg caataggca agccatgca 
      aaggaagcg aagtctgag atcaaacaa aagcactga 
      cgctgtttc gaatggtaa tagtcagga tagcgctaa 
      agcaaaagc tagttcaag cgttgacaa taatgacag 
      ttgtggctc acgcccact ttcgctccg ccttttacc 
      tgaacatgt gtacaatca acaaccccc tcccccatg 
      tcttgtgta cggtgccat tcatttgac gcgactttt 
      tgtcaaatt gcccgaatt gttgaacgc agccaccat 
      tgatgagaa aaaatggac aactttatt gcaacacat 
      ggacacttt tattgatta cttaaacga gcatcggga 
      gtggtttgg ggaaaagtt ctacatcga caggcaata 
      aaacaaggc gtttttaat agaagatgt attacctac 
      aagtaaata tttggtaac aaataaata tgtcattat 
      tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY