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Gene information
General information
TC007094 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9628012..9629993
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007094
    iBeetle screen iB_07241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    labium sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax symmetry irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax partially unilateralfused - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007094 RA
      MEDIKTKSI HHTAIKICE DFKSSPSYQ ALFQDLQKL 
      VSTPSVKYD DFKESLLKA MKDNGLDAG LRNNVYHWM 
      KTKKKEVSS PYDAFFRKA QIHWDKRIH KSLNSMCAE 
      LGINLAKAR TVAEREEIA SKWGELSNY EVDIHKYRP 
      VYAPKDFLE VLLNLKGPT TRTKGCPKW EFAQMPLKV 
      KNLTDLRCL YLELSRGEQ ILGINCSMM SPHYSTLES 
      ERITLGEKV LAKNYAPMA QEFLKKGCP QCLRAKIWT 
      LVLGADVKP YHYSYFESL KESVLQYDL MIDKLIIKD 
      INLTASNDD QYFVFEDVL YQVMLCFSR DGEILKDLS 
      TQPGFMQVV IKGKQNGAE NTIIFPPSG VIPFHGFTM 
      YAAPFCYLY DDPIQLYFV FRTFYIRYF HRLHRVCGN 
      PQGVVALCL QYEKMLQCF EPSLWHHFR KCQIHPVRV 
      VIKWIMRAF SGHLPPEQL LNLWDLILA YDSLEIIPL 
      LALVILVFR KENLLKVNT LQNIEAVLA DLSSISVIP 
      LLQMALLKE
    CDS sequence
    • >TC007094 RA
      atggaagac atcaaaacc aaaagcatt caccacact 
      gccataaaa atctgtgaa gacttcaaa tcgtctccc 
      tcttatcag gccctcttc caggatcta cagaaatta 
      gtgtccacc cccagtgta aaatatgac gatttcaag 
      gaaagtcta ctcaaggcc atgaaagat aacggcctg 
      gacgccggt ttgcgaaac aatgtgtat cattggatg 
      aaaacaaag aagaaagaa gtgtcttcg ccttacgac 
      gcgtttttc cgaaaagca caaattcac tgggataaa 
      agaatccac aaatcactg aactcgatg tgtgccgaa 
      cttgggatc aaccttgcg aaggcgcga actgtggca 
      gaaagggag gaaattgcg agtaaatgg ggcgaactt 
      agcaattat gaggtcgac attcataaa tacagaccg 
      gtgtatgcg ccgaaagac tttctggaa gttttgcta 
      aacctcaaa ggaccaaca acgaggact aaaggttgt 
      ccgaaatgg gaatttgcg caaatgcct ctcaaagtc 
      aagaattta acggacttg agatgtctc tacttggag 
      ctttcaaga ggcgagcaa atcttgggc atcaactgc 
      tcaatgatg tctccacac tacagtacg ttagaaagc 
      gaacgcata acacttggt gaaaaagtc ttagctaag 
      aattacgcc cccatggcg caagaattc ctgaaaaag 
      ggctgcccc cagtgcttg agggccaaa atctggact 
      ttagtttta ggagccgat gttaaacct taccattat 
      tcatacttc gagtcgctg aaagaaagc gtcctccag 
      tacgactta atgatcgat aaactgatc atcaaagac 
      atcaacctg acggcatca aacgacgat caatatttc 
      gtgtttgag gatgttttg taccaggtg atgttgtgt 
      ttttcgcgc gatggagaa atcctgaaa gacctctcc 
      actcaaccc ggattcatg caagtcgtg atcaaaggc 
      aaacagaac ggtgctgaa aacacgatt attttccca 
      ccaagtggc gtaatccca tttcacggc ttcacaatg 
      tacgctgcc cctttttgt tacttgtac gacgacccg 
      atccaactg tatttcgtg tttcgcact ttttacatt 
      cgttacttc caccgtttg catcgcgtg tgtggaaat 
      ccgcaagga gtggttgcg ttgtgtctt cagtatgag 
      aaaatgttg cagtgtttt gagcccagt ctttggcac 
      cactttcga aaatgtcaa attcatccc gttcgagtg 
      gtaattaag tggataatg cgcgcgttc agtggacat 
      ctaccacct gaacagttg ttgaattta tgggatttg 
      attctggcg tatgattcg ctcgagatt attccgctg 
      ttggcgctt gtgattcta gtttttcgg aaagagaat 
      ttgttgaaa gtgaatacg ttgcaaaat attgaagct 
      gttttggcg gatttgtcg tcgatttct gttattccg 
      ttgctgcaa atggcgctt ttgaaagaa tga
    mRNA sequence
    • >TC007094 RA
      agtgtttga taagacgtc gacaatgga agacatcaa 
      aaccaaaag cattcacca cactgccat aaaaatctg 
      tgaagactt caaatcgtc tccctctta tcaggccct 
      cttccagga tctacagaa attagtgtc cacccccag 
      tgtaaaata tgacgattt caaggaaag tctactcaa 
      ggccatgaa agataacgg cctggacgc cggtttgcg 
      aaacaatgt gtatcattg gatgaaaac aaagaagaa 
      agaagtgtc ttcgcctta cgacgcgtt tttccgaaa 
      agcacaaat tcactggga taaaagaat ccacaaatc 
      actgaactc gatgtgtgc cgaacttgg gatcaacct 
      tgcgaaggc gcgaactgt ggcagaaag ggaggaaat 
      tgcgagtaa atggggcga acttagcaa ttatgaggt 
      cgacattca taaatacag accggtgta tgcgccgaa 
      agactttct ggaagtttt gctaaacct caaaggacc 
      aacaacgag gactaaagg ttgtccgaa atgggaatt 
      tgcgcaaat gcctctcaa agtcaagaa tttaacgga 
      cttgagatg tctctactt ggagctttc aagaggcga 
      gcaaatctt gggcatcaa ctgctcaat gatgtctcc 
      acactacag tacgttaga aagcgaacg cataacact 
      tggtgaaaa agtcttagc taagaatta cgcccccat 
      ggcgcaaga attcctgaa aaagggctg cccccagtg 
      cttgagggc caaaatctg gactttagt tttaggagc 
      cgatgttaa accttacca ttattcata cttcgagtc 
      gctgaaaga aagcgtcct ccagtacga cttaatgat 
      cgataaact gatcatcaa agacatcaa cctgacggc 
      atcaaacga cgatcaata tttcgtgtt tgaggatgt 
      tttgtacca ggtgatgtt gtgtttttc gcgcgatgg 
      agaaatcct gaaagacct ctccactca acccggatt 
      catgcaagt cgtgatcaa aggcaaaca gaacggtgc 
      tgaaaacac gattatttt cccaccaag tggcgtaat 
      cccatttca cggcttcac aatgtacgc tgccccttt 
      ttgttactt gtacgacga cccgatcca actgtattt 
      cgtgtttcg cacttttta cattcgtta cttccaccg 
      tttgcatcg cgtgtgtgg aaatccgca aggagtggt 
      tgcgttgtg tcttcagta tgagaaaat gttgcagtg 
      ttttgagcc cagtctttg gcaccactt tcgaaaatg 
      tcaaattca tcccgttcg agtggtaat taagtggat 
      aatgcgcgc gttcagtgg acatctacc acctgaaca 
      gttgttgaa tttatggga tttgattct ggcgtatga 
      ttcgctcga gattattcc gctgttggc gcttgtgat 
      tctagtttt tcggaaaga gaatttgtt gaaagtgaa 
      tacgttgca aaatattga agctgtttt ggcggattt 
      gtcgtcgat ttctgttat tccgttgct gcaaatggc 
      gcttttgaa agaatgagt tgttggaaa tggtcgatc 
      actgccatt ttgtaattt tgtattgtt atattgtat 
      tggtctaat aaagttgat agataaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY