General information | |||
TC007094 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9628012..9629993 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC007094 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07241 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
labium sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head fused - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax symmetry irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
legs partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax partially unilateralfused - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax fused - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007094 RA
MEDIKTKSI HHTAIKICE DFKSSPSYQ ALFQDLQKL VSTPSVKYD DFKESLLKA MKDNGLDAG LRNNVYHWM KTKKKEVSS PYDAFFRKA QIHWDKRIH KSLNSMCAE LGINLAKAR TVAEREEIA SKWGELSNY EVDIHKYRP VYAPKDFLE VLLNLKGPT TRTKGCPKW EFAQMPLKV KNLTDLRCL YLELSRGEQ ILGINCSMM SPHYSTLES ERITLGEKV LAKNYAPMA QEFLKKGCP QCLRAKIWT LVLGADVKP YHYSYFESL KESVLQYDL MIDKLIIKD INLTASNDD QYFVFEDVL YQVMLCFSR DGEILKDLS TQPGFMQVV IKGKQNGAE NTIIFPPSG VIPFHGFTM YAAPFCYLY DDPIQLYFV FRTFYIRYF HRLHRVCGN PQGVVALCL QYEKMLQCF EPSLWHHFR KCQIHPVRV VIKWIMRAF SGHLPPEQL LNLWDLILA YDSLEIIPL LALVILVFR KENLLKVNT LQNIEAVLA DLSSISVIP LLQMALLKE
- >TC007094 RA
atggaagac atcaaaacc aaaagcatt caccacact gccataaaa atctgtgaa gacttcaaa tcgtctccc tcttatcag gccctcttc caggatcta cagaaatta gtgtccacc cccagtgta aaatatgac gatttcaag gaaagtcta ctcaaggcc atgaaagat aacggcctg gacgccggt ttgcgaaac aatgtgtat cattggatg aaaacaaag aagaaagaa gtgtcttcg ccttacgac gcgtttttc cgaaaagca caaattcac tgggataaa agaatccac aaatcactg aactcgatg tgtgccgaa cttgggatc aaccttgcg aaggcgcga actgtggca gaaagggag gaaattgcg agtaaatgg ggcgaactt agcaattat gaggtcgac attcataaa tacagaccg gtgtatgcg ccgaaagac tttctggaa gttttgcta aacctcaaa ggaccaaca acgaggact aaaggttgt ccgaaatgg gaatttgcg caaatgcct ctcaaagtc aagaattta acggacttg agatgtctc tacttggag ctttcaaga ggcgagcaa atcttgggc atcaactgc tcaatgatg tctccacac tacagtacg ttagaaagc gaacgcata acacttggt gaaaaagtc ttagctaag aattacgcc cccatggcg caagaattc ctgaaaaag ggctgcccc cagtgcttg agggccaaa atctggact ttagtttta ggagccgat gttaaacct taccattat tcatacttc gagtcgctg aaagaaagc gtcctccag tacgactta atgatcgat aaactgatc atcaaagac atcaacctg acggcatca aacgacgat caatatttc gtgtttgag gatgttttg taccaggtg atgttgtgt ttttcgcgc gatggagaa atcctgaaa gacctctcc actcaaccc ggattcatg caagtcgtg atcaaaggc aaacagaac ggtgctgaa aacacgatt attttccca ccaagtggc gtaatccca tttcacggc ttcacaatg tacgctgcc cctttttgt tacttgtac gacgacccg atccaactg tatttcgtg tttcgcact ttttacatt cgttacttc caccgtttg catcgcgtg tgtggaaat ccgcaagga gtggttgcg ttgtgtctt cagtatgag aaaatgttg cagtgtttt gagcccagt ctttggcac cactttcga aaatgtcaa attcatccc gttcgagtg gtaattaag tggataatg cgcgcgttc agtggacat ctaccacct gaacagttg ttgaattta tgggatttg attctggcg tatgattcg ctcgagatt attccgctg ttggcgctt gtgattcta gtttttcgg aaagagaat ttgttgaaa gtgaatacg ttgcaaaat attgaagct gttttggcg gatttgtcg tcgatttct gttattccg ttgctgcaa atggcgctt ttgaaagaa tga
- >TC007094 RA
agtgtttga taagacgtc gacaatgga agacatcaa aaccaaaag cattcacca cactgccat aaaaatctg tgaagactt caaatcgtc tccctctta tcaggccct cttccagga tctacagaa attagtgtc cacccccag tgtaaaata tgacgattt caaggaaag tctactcaa ggccatgaa agataacgg cctggacgc cggtttgcg aaacaatgt gtatcattg gatgaaaac aaagaagaa agaagtgtc ttcgcctta cgacgcgtt tttccgaaa agcacaaat tcactggga taaaagaat ccacaaatc actgaactc gatgtgtgc cgaacttgg gatcaacct tgcgaaggc gcgaactgt ggcagaaag ggaggaaat tgcgagtaa atggggcga acttagcaa ttatgaggt cgacattca taaatacag accggtgta tgcgccgaa agactttct ggaagtttt gctaaacct caaaggacc aacaacgag gactaaagg ttgtccgaa atgggaatt tgcgcaaat gcctctcaa agtcaagaa tttaacgga cttgagatg tctctactt ggagctttc aagaggcga gcaaatctt gggcatcaa ctgctcaat gatgtctcc acactacag tacgttaga aagcgaacg cataacact tggtgaaaa agtcttagc taagaatta cgcccccat ggcgcaaga attcctgaa aaagggctg cccccagtg cttgagggc caaaatctg gactttagt tttaggagc cgatgttaa accttacca ttattcata cttcgagtc gctgaaaga aagcgtcct ccagtacga cttaatgat cgataaact gatcatcaa agacatcaa cctgacggc atcaaacga cgatcaata tttcgtgtt tgaggatgt tttgtacca ggtgatgtt gtgtttttc gcgcgatgg agaaatcct gaaagacct ctccactca acccggatt catgcaagt cgtgatcaa aggcaaaca gaacggtgc tgaaaacac gattatttt cccaccaag tggcgtaat cccatttca cggcttcac aatgtacgc tgccccttt ttgttactt gtacgacga cccgatcca actgtattt cgtgtttcg cacttttta cattcgtta cttccaccg tttgcatcg cgtgtgtgg aaatccgca aggagtggt tgcgttgtg tcttcagta tgagaaaat gttgcagtg ttttgagcc cagtctttg gcaccactt tcgaaaatg tcaaattca tcccgttcg agtggtaat taagtggat aatgcgcgc gttcagtgg acatctacc acctgaaca gttgttgaa tttatggga tttgattct ggcgtatga ttcgctcga gattattcc gctgttggc gcttgtgat tctagtttt tcggaaaga gaatttgtt gaaagtgaa tacgttgca aaatattga agctgtttt ggcggattt gtcgtcgat ttctgttat tccgttgct gcaaatggc gcttttgaa agaatgagt tgttggaaa tggtcgatc actgccatt ttgtaattt tgtattgtt atattgtat tggtctaat aaagttgat agataaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
