General information | |||
TC007099 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9578553..9583281 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC007099 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07239 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%
larva fragmented - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
mesothorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
metathorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007099 RA
MSRLILKSG ASFAQTFQN CGFLSVFPT NFGAQKLKL REQRRFMSA GIVQSPLGP SKKIPNENL VEYIYKNND KWIEEPAMT CGVSGRSYT YGMLRMLIN RCAQALLGH CGMKPREVV GLLLPNIPE YAVVCHGAI EAGLVVTFV NPLYTPDEI KRQFENAGV KMIVTVPQL LEVALTIAP QLQEYRTTI CIGGEDDPS KNVNGLQSM LMAGHEAEL PGINPREIA ILPYSSGTT GLPKGVMLS HYNLVANLV QGEHPALED LETKDGKRH TMLTVLPFF HIYGFNGIL NLCLKNGAH IITIPRFTP EDYLKTLVE YKPSFIFVV PSLLLFLAS HPAVTKEHL SSIEAVQSG AAPLTEGLL QKFRQKVGR DDILIRQGY GMTESSPVT FCMPKLTPP SKIATIGLP YPGTEAKVI SLSNGEPQG THKSGELLV RGPQIMMGY LNNEQATAE TVDEEGWLH TGDVAYYDE DFYFYIVDR CKELIKVKG NQVSFF
- >TC007099 RA
atgtcgcgt ttaatttta aaaagtggt gcatccttc gcgcaaacc ttccaaaat tgtggattc ctctccgtt tttccgacc aacttcggg gcccaaaaa ctgaaactg cgtgaacag aggcgattt atgagtgct ggaatcgtc cagtcgcct ctgggacca agcaaaaaa ataccaaac gaaaacctc gtcgagtac atttacaaa aataacgac aaatggatc gaggagccc gccatgact tgtggcgtt tccgggcga agctacacc tacggaatg ctccggatg ctgatcaac agatgtgcc caggccctt ttgggccac tgcggcatg aaaccgcga gaagtcgtc ggtttgctt ctaccaaac attcccgaa tacgccgta gtgtgtcat ggggccatc gaggccgga cttgtggtc acttttgtc aacccttta tacactcct gatgaaatc aaacgccag tttgagaat gccggagtc aagatgatt gttacagtg cctcaattg ctagaggtg gcgttaaca atagcccca caattgcaa gagtatcga actacgatt tgtatcgga ggagaggac gatccctcg aagaacgtt aacgggctt cagagcatg ttaatggcg gggcatgaa gccgaactt cccgggatt aatcccaga gagattgcc attttgccg tattcgagt ggcacgacg ggattgccg aagggagta atgctctcg cattataat ttagtggcg aatttggtg caaggggaa cacccggct ttggaggac ttggaaact aaagatggg aaaaggcac accatgcta acagtcctt cccttcttc cacatttac ggcttcaat ggaattttg aacttgtgc ctcaaaaac ggcgctcac atcatcacc attccacgg tttactccc gaagattac ctcaaaacc ctcgtcgaa tacaaaccg agctttatt ttcgtcgtt ccttccctg ctcttgttc ctggcctcg catcctgcc gtcaccaag gaacacttg agttctatt gaagccgtc cagtcagga gcggccccc ttgactgaa ggtctcttg caaaaattc agacagaag gtgggaaga gacgatatt ctgataaga caaggttac ggcatgacg gagagttcg ccggttact ttctgtatg cccaagttg acgccaccg tcgaaaatt gcaacgatt ggacttcct tatcccggg acggaagca aaagttatt agtttgtca aatggggaa ccgcagggg acacacaag tcgggtgaa ttgctagtg agggggcca cagattatg atggggtac ttgaacaac gaacaagcc actgcggag actgtcgat gaggaaggg tggctccat acaggggat gtggcctat tacgacgaa gatttctac ttttacatt gtcgatagg tgcaaggaa ctcatcaaa gtcaagggc aatcaagta agttttttt taa
- >TC007099 RA
tactttgtg taaaaagtg atatacgtc agtgttgga taaataaga aacacgtgt gtttggcat cttttggtc tgatttacc atcaatcaa actttgcac caaacaatt aatgataat ggaaatggc gcaaacaaa acgattcaa tgcaatgta aagtcctta ttcattctt tgcggccaa tctagggct tattacaat aagtgaaag cgtcagaaa gttaccgct gctattttt ctcggcgtt cagccgctt gtttgatca gaaatatcc gaatggtga gaagttgga ctttgaatc attggactc cgtacaagt tatctattt tattttaat taaatgtac tcgcggatc cattcaaaa caatcatag gtgcaataa agacaacac ggttgcggt gtgctcaaa accagtgcg agtgttaat taacccacc ttcaagatt tttcactat tttccaagg tagcacacc tatttttac cggggaata aatatgtcg cgtttaatt ttaaaaagt ggtgcatcc ttcgcgcaa accttccaa aattgtgga ttcctctcc gtttttccg accaacttc ggggcccaa aaactgaaa ctgcgtgaa cagaggcga tttatgagt gctggaatc gtccagtcg cctctggga ccaagcaaa aaaatacca aacgaaaac ctcgtcgag tacatttac aaaaataac gacaaatgg atcgaggag cccgccatg acttgtggc gtttccggg cgaagctac acctacgga atgctccgg atgctgatc aacagatgt gcccaggcc cttttgggc cactgcggc atgaaaccg cgagaagtc gtcggtttg cttctacca aacattccc gaatacgcc gtagtgtgt catggggcc atcgaggcc ggacttgtg gtcactttt gtcaaccct ttatacact cctgatgaa atcaaacgc cagtttgag aatgccgga gtcaagatg attgttaca gtgcctcaa ttgctagag gtggcgtta acaatagcc ccacaattg caagagtat cgaactacg atttgtatc ggaggagag gacgatccc tcgaagaac gttaacggg cttcagagc atgttaatg gcggggcat gaagccgaa cttcccggg attaatccc agagagatt gccattttg ccgtattcg agtggcacg acgggattg ccgaaggga gtaatgctc tcgcattat aatttagtg gcgaatttg gtgcaaggg gaacacccg gctttggag gacttggaa actaaagat gggaaaagg cacaccatg ctaacagtc cttcccttc ttccacatt tacggcttc aatggaatt ttgaacttg tgcctcaaa aacggcgct cacatcatc accattcca cggtttact cccgaagat tacctcaaa accctcgtc gaatacaaa ccgagcttt attttcgtc gttccttcc ctgctcttg ttcctggcc tcgcatcct gccgtcacc aaggaacac ttgagttct attgaagcc gtccagtca ggagcggcc cccttgact gaaggtctc ttgcaaaaa ttcagacag aaggtggga agagacgat attctgata agacaaggt tacggcatg acggagagt tcgccggtt actttctgt atgcccaag ttgacgcca ccgtcgaaa attgcaacg attggactt ccttatccc gggacggaa gcaaaagtt attagtttg tcaaatggg gaaccgcag gggacacac aagtcgggt gaattgcta gtgaggggg ccacagatt atgatgggg tacttgaac aacgaacaa gccactgcg gagactgtc gatgaggaa gggtggctc catacaggg gatgtggcc tattacgac gaagatttc tacttttac attgtcgat aggtgcaag gaactcatc aaagtcaag ggcaatcaa gtaagtttt ttttaatta gctcaaaag gtaagtaca gattttatc accactaat aagtggata agcgcaaat cttacgaat tcgcgtcta ttcaaagtt aatttacaa caaaatgta gccaaaaag gacaagact ctgctctac ttgttagat ttaaactaa ctgtagaac gtagggaaa agaatcata attattaca catacatat tacgttacc ttaaataca aggtgttta aacgtaatt tacgcctat aaaatgcaa tcagcaata catataatt taagaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
