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Gene information
General information
TC007111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9226700..9234708
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007111
    iBeetle screen iB_07237

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber potentially not present - Number of animals showing the phenotype: 2

    vitellogenic egg chamber potentially shape irregular - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09944

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007111 RA
      MSWPQWSAA PQPSVPPVL NPTTPVINS APSVVPPGL 
      QYTPEQWAQ LQQQNWNQW AQWQQQYQQ WHQQYGAEY 
      QKSINALAQ AAQVQQTPA LPNVAVPPP LPADNRPPP 
      PVDEPPPPS YTSAPPPSH GYNTAPPLK TMRQSWGNQ 
      NYRFEQQKR NYEQPDDVN KRPMVNKPG WQQPRPSKD 
      NLEELSEAE KKFDKQFAE WEAQFNKWK EQNAEHPDK 
      EQYREYEKK WESWRAQLL ERREQMKRK RLGLAVDSP 
      SKSRPGSGQ SFNSPQANF NQPPPKVLP SVPPKEGLL 
      PLPQPSFNK PPPTLNDEP LKFKDSKPV PELDDSVSS 
      FFKPNTGGG IPGLDLVKD DPKPDKSAT EDVVLVDPE 
      EKEREKKGP DFDAISKGI NTILGDQKL LNMLSNSQI 
      KVSRIIPIM GKVTHKENL LILMIKLGA ASRWLATTR 
      KRDSMDLEV SSKRIKISF QIDLGILIK EIALGVSTK 
      GKAILVQDP TKGFHQEAR ISLDFHQIK IKIGLVQIK 
      ISLPRIKIS LVQIKIGLV RTKISLVQV RTGLVRIGL 
      ALIKAETGR TNGLGLIRI GLVLIRASL VRIRISLGR 
      IRNNLVRIS RDSVRTKTG LDLIKIDLA LVRIRDRNF 
      SRGPPQKGQ FNYNNDNNF NQYDNDQGY NEEEYEENY 
      DYDQVASNF EEETSHSMD WKEEENYED QNQPPVVEQ 
      EPPEAPKIT GIFQPSEVI DYEHKPPKL GEPEVLIEP 
      IYMFDYRHK PLSRIPIPQ RPKWLADVV KNIREFDPP 
      TVRPYEPNL RRYPPPMDH WRKDRYQEN WSPHNRRSY 
      NDDRRPYGD RYDEPPMEK RREDRRSYP EEKKQFNRM 
      ELEEEKISD TEWPDDDFE AEKPNFTKF QKPDKPTKN 
      VVLIEDLIS PPGRFKRPP RIVIILRGP PGSGKTFLA 
      KLIKDKEVE NGGSAPRIL SLDDYFMVE QEKEVTEEG 
      KKVKIKEMV YEYEAEMEE SYRTSLIKS FKKTITDGY 
      FSFIIVDNV NDKVKYFGE MWSYAKQYG FQVYICQLD 
      LDVHTCSKR NIHGRSEAD IERCVSNWE PTPQHHPIL 
      DATSLLQSA SIPEVEMEE VNSPESDLN ECDGSEDQV 
      ARSKWDTFD CSVDNLAKL DGVSKPLRH RTMEEYLQL 
      DTEWTPIKP TKPGQKRVR WADLEEQKQ QEKMRAIGF 
      VVGHTDWDR MMDPTGGSS ALTQTKYIE RTGRFKVDQ 
      K
    CDS sequence
    • >TC007111 RA
      atgtcttgg ccccagtgg tccgcagcc cctcagccg 
      tcagtcccc ccagtgctg aacccaacc actcccgtt 
      ataaattca gccccgagt gtggttccc cctggttta 
      caatacact ccagagcag tgggcccaa ctgcaacaa 
      caaaactgg aaccagtgg gcgcagtgg caacaacag 
      taccaacag tggcaccaa caatacggt gccgagtac 
      cagaaatcg ataaatgct cttgctcaa gcagcccaa 
      gtacagcaa acgccagcg ttgccaaat gtggcagtg 
      ccgccccct ttaccggcc gataatcgg ccgccgcca 
      ccggtggac gagccgccg cctccgtcc tatacttcc 
      gcgccgcct ccaagtcac ggttacaac accgcacct 
      ccgctaaaa acaatgcgc caaagttgg ggcaatcag 
      aattataga tttgaacaa caaaagaga aattatgag 
      caaccagat gatgttaat aagcgacca atggtcaat 
      aagcctgga tggcagcaa ccacggcct tctaaagac 
      aatctcgag gagttgtcc gaagcggag aaaaaattc 
      gataaacag tttgcggaa tgggaggca caatttaat 
      aaatggaag gaacaaaat gcggaacat cccgataaa 
      gagcagtac cgggagtat gagaaaaaa tgggagagt 
      tggagggca caactcctg gaaaggagg gagcaaatg 
      aagaggaag agattggga ttggctgtg gattcgccc 
      agtaaaagt agaccagga tcggggcaa agttttaat 
      tctcctcaa gcaaacttt aaccaacct ccacctaaa 
      gtattacct agtgttcct ccaaaagaa gggctactt 
      ccgcttcct caaccctct tttaataaa ccaccaccg 
      actttaaac gatgagcct ttgaaattt aaggactcc 
      aaacctgtg cccgaattg gacgattct gtgtccagt 
      tttttcaaa cctaacact gggggcgga atcccaggc 
      ttagacttg gttaaagat gatccaaaa cctgacaaa 
      agtgcaact gaagatgtt gtgctcgtt gatcctgaa 
      gaaaaggag agagagaag aaaggacct gactttgat 
      gccatttcc aagggtatt aatacgatt ctcggagac 
      caaaaactg cttaatatg ttatcgaac agtcaaatc 
      aaagtttcg aggatcatt ccaatcatg gggaaagtt 
      acgcacaaa gagaatttg ttaattttg atgatcaaa 
      ctcggagca gcttcacga tggttagca acgaccagg 
      aaacgagat tcgatggac ctggaagtt tcgtcgaaa 
      agaatcaaa atcagtttc caaatcgat ttgggaatt 
      ttaatcaag gaaatcgct ttgggagtt tcgacgaag 
      ggaaaagca attttggtg caagacccg acgaaaggt 
      ttccaccag gaggcccga atcagtcta gatttccac 
      caaatcaag atcaaaata ggtttggtc caaatcaag 
      ataagtttg ccccgaatc aagatcagt ttggtccaa 
      atcaaaata ggtttggtc cgaaccaag ataagtttg 
      gtccaggtc aggacaggt ttggtccga ataggtttg 
      gccctaatc aaggcagaa acgggccga accaacggt 
      ttgggccta atcagaata ggtttggtc ctaatcagg 
      gcaagtttg gtccgaatc aggataagt ttgggccga 
      atcaggaac aatttggtc cgaatcagc agagattcg 
      gtccgaacc aaaacaggt ttggaccta atcaaaata 
      gatttggcc ctggtccga atcagggat cggaatttt 
      tcaagaggt ccgccccag aaaggacag tttaattat 
      aataacgat aataacttc aatcagtat gacaatgat 
      cagggatac aatgaagaa gagtacgag gaaaattac 
      gattacgat caagtggcg tcaaatttt gaggaagaa 
      acaagtcat tcaatggac tggaaagaa gaagaaaat 
      tatgaagat caaaaccaa ccgccagta gttgagcaa 
      gaaccgcct gaagcaccg aaaataact ggaattttt 
      caaccgtcg gaagttatc gattatgag cataaacct 
      cccaaactt ggggaaccg gaagttctg atcgaacct 
      atttacatg tttgactac cgtcacaaa ccattgagt 
      cgcattcca attccgcag aggcctaaa tggcttgct 
      gatgtcgtt aaaaacata agagaattt gatccgcca 
      actgttagg ccttacgaa ccaaacttg cgaaggtat 
      cctccacct atggatcat tggaggaaa gatcgctac 
      caagaaaac tggtcgccc cataataga cgaagttac 
      aatgacgac agacgaccc tatggtgat agatacgac 
      gagccacca atggagaaa aggagagaa gacaggagg 
      agttatccg gaagagaag aaacagttt aatagaatg 
      gagttggag gaagagaaa ataagtgat acggagtgg 
      ccagacgat gattttgaa gcggaaaaa ccgaatttt 
      acgaaattt caaaaaccg gacaaacct acaaaaaat 
      gttgtcttg atcgaggat ttgatcagt cctcctggc 
      aggtttaag cggcctcca cgcatcgta ataatcctg 
      agaggtcct ccagggagt ggcaaaacg ttccttgcg 
      aaactcatc aaagataag gaggtggag aatggcggt 
      tcggcgcca cgtatttta tcccttgat gattatttt 
      atggtcgaa caagagaaa gaagtgact gaagagggc 
      aaaaaagtg aagattaaa gaaatggtt tacgagtac 
      gaggccgaa atggaggag tcctaccgg acttctttg 
      atcaaatcg ttcaagaaa accataaca gacggttac 
      ttctcgttt attattgtg gacaatgtc aacgacaaa 
      gtgaaatat ttcggtgaa atgtggagt tatgcgaaa 
      cagtacggt tttcaggtt tacatttgt cagttagat 
      ttggacgtt cacacttgt tcgaaacga aatattcac 
      ggacgatct gaggctgac atcgaaagg tgtgtctca 
      aactgggaa cccactcct caacatcac cccattctc 
      gacgccaca agtctcctc caatcagcg tcgattccc 
      gaagtcgaa atggaagaa gttaactca cctgaaagt 
      gatctgaac gaatgtgac gggtcggag gaccaagta 
      gctagaagc aagtgggac actttcgac tgttctgtt 
      gacaatctc gcgaaactc gatggcgtc agcaaaccc 
      ttacgacac cggaccatg gaagaatac ctccaattg 
      gatacagag tggactccc atcaaaccc accaaaccc 
      gggcaaaag agagtcaga tgggccgat ttggaagaa 
      cagaaacaa caagagaag atgagagca attggattt 
      gtcgttgga cacacagac tgggaccga atgatggat 
      ccgacaggg ggcagttct gcgctaact caaacgaaa 
      tatatcgaa cgaacggga cggtttaaa gttgatcag 
      aaataa
    mRNA sequence
    • >TC007111 RA
      tgcatgaat ttaattaaa atagggaaa aaacgttaa 
      gggtattga tttgaggtt atgtaatga attatcaat 
      tttttctaa ataactttt ttattaatg aacagaact 
      acacaaaaa cgaattaaa taacattct aattattat 
      atacagcaa tcagtccct agattcgtg tcttccact 
      taatttaag atttctttt ctgcatctt ccttttcct 
      tcgccaaca aaagcgcac ttttgatgc caaccttct 
      ccaactcct cgatcagat cccccgact ggtgcattt 
      gcagcatta tatcacagc cccccacaa attcgccgt 
      tgataaaaa cctgtggta ttgtgggcc aatttgaaa 
      aatccttaa taccctgcc gtagcgact catcagaca 
      ggacatcat gggcttcat actgcaccc catgcattc 
      gtaaaattt gcacaaccg cattgctaa agccacacc 
      ggggctgtt caggaaccc ccttcataa aaaccacaa 
      ctttgttat tgttgacca tttttttaa tttcttcgg 
      ccggttttg tgctataaa acctagcga gagtggtcg 
      cattttgta acaggtttc tactctttg tgaaaaaat 
      ttaaactca ttttttgtt tgaatttgt gtaacctca 
      cttttagac agatagggg gtggaaact agagatttt 
      cgttccgtg ttggcaacc ttgttgatt gcttgacaa 
      gaaacaggt tgacaggaa tgagccgca aaattaata 
      aattattgt tttttgcca atatttcaa acagcaatt 
      caggtatct caagttttt ttctaacta attggcgtt 
      ctctaccag tgttggttt ttcattgtt tcaaaaaca 
      acgtttttt atggtttta gcgtccaca gaatagatt 
      tattggaat aagttgagg caatgtctt ggccccagt 
      ggtccgcag cccctcagc cgtcagtcc ccccagtgc 
      tgaacccaa ccactcccg ttataaatt cagccccga 
      gtgtggttc cccctggtt tacaataca ctccagagc 
      agtgggccc aactgcaac aacaaaact ggaaccagt 
      gggcgcagt ggcaacaac agtaccaac agtggcacc 
      aacaatacg gtgccgagt accagaaat cgataaatg 
      ctcttgctc aagcagccc aagtacagc aaacgccag 
      cgttgccaa atgtggcag tgccgcccc ctttaccgg 
      ccgataatc ggccgccgc caccggtgg acgagccgc 
      cgcctccgt cctatactt ccgcgccgc ctccaagtc 
      acggttaca acaccgcac ctccgctaa aaacaatgc 
      gccaaagtt ggggcaatc agaattata gatttgaac 
      aacaaaaga gaaattatg agcaaccag atgatgtta 
      ataagcgac caatggtca ataagcctg gatggcagc 
      aaccacggc cttctaaag acaatctcg aggagttgt 
      ccgaagcgg agaaaaaat tcgataaac agtttgcgg 
      aatgggagg cacaattta ataaatgga aggaacaaa 
      atgcggaac atcccgata aagagcagt accgggagt 
      atgagaaaa aatgggaga gttggaggg cacaactcc 
      tggaaagga gggagcaaa tgaagagga agagattgg 
      gattggctg tggattcgc ccagtaaaa gtagaccag 
      gatcggggc aaagtttta attctcctc aagcaaact 
      ttaaccaac ctccaccta aagtattac ctagtgttc 
      ctccaaaag aagggctac ttccgcttc ctcaaccct 
      cttttaata aaccaccac cgactttaa acgatgagc 
      ctttgaaat ttaaggact ccaaacctg tgcccgaat 
      tggacgatt ctgtgtcca gttttttca aacctaaca 
      ctgggggcg gaatcccag gcttagact tggttaaag 
      atgatccaa aacctgaca aaagtgcaa ctgaagatg 
      ttgtgctcg ttgatcctg aagaaaagg agagagaga 
      agaaaggac ctgactttg atgccattt ccaagggta 
      ttaatacga ttctcggag accaaaaac tgcttaata 
      tgttatcga acagtcaaa tcaaagttt cgaggatca 
      ttccaatca tggggaaag ttacgcaca aagagaatt 
      tgttaattt tgatgatca aactcggag cagcttcac 
      gatggttag caacgacca ggaaacgag attcgatgg 
      acctggaag tttcgtcga aaagaatca aaatcagtt 
      tccaaatcg atttgggaa ttttaatca aggaaatcg 
      ctttgggag tttcgacga agggaaaag caattttgg 
      tgcaagacc cgacgaaag gtttccacc aggaggccc 
      gaatcagtc tagatttcc accaaatca agatcaaaa 
      taggtttgg tccaaatca agataagtt tgccccgaa 
      tcaagatca gtttggtcc aaatcaaaa taggtttgg 
      tccgaacca agataagtt tggtccagg tcaggacag 
      gtttggtcc gaataggtt tggccctaa tcaaggcag 
      aaacgggcc gaaccaacg gtttgggcc taatcagaa 
      taggtttgg tcctaatca gggcaagtt tggtccgaa 
      tcaggataa gtttgggcc gaatcagga acaatttgg 
      tccgaatca gcagagatt cggtccgaa ccaaaacag 
      gtttggacc taatcaaaa tagatttgg ccctggtcc 
      gaatcaggg atcggaatt tttcaagag gtccgcccc 
      agaaaggac agtttaatt ataataacg ataataact 
      tcaatcagt atgacaatg atcagggat acaatgaag 
      aagagtacg aggaaaatt acgattacg atcaagtgg 
      cgtcaaatt ttgaggaag aaacaagtc attcaatgg 
      actggaaag aagaagaaa attatgaag atcaaaacc 
      aaccgccag tagttgagc aagaaccgc ctgaagcac 
      cgaaaataa ctggaattt ttcaaccgt cggaagtta 
      tcgattatg agcataaac ctcccaaac ttggggaac 
      cggaagttc tgatcgaac ctatttaca tgtttgact 
      accgtcaca aaccattga gtcgcattc caattccgc 
      agaggccta aatggcttg ctgatgtcg ttaaaaaca 
      taagagaat ttgatccgc caactgtta ggccttacg 
      aaccaaact tgcgaaggt atcctccac ctatggatc 
      attggagga aagatcgct accaagaaa actggtcgc 
      cccataata gacgaagtt acaatgacg acagacgac 
      cctatggtg atagatacg acgagccac caatggaga 
      aaaggagag aagacagga ggagttatc cggaagaga 
      agaaacagt ttaatagaa tggagttgg aggaagaga 
      aaataagtg atacggagt ggccagacg atgattttg 
      aagcggaaa aaccgaatt ttacgaaat ttcaaaaac 
      cggacaaac ctacaaaaa atgttgtct tgatcgagg 
      atttgatca gtcctcctg gcaggttta agcggcctc 
      cacgcatcg taataatcc tgagaggtc ctccaggga 
      gtggcaaaa cgttccttg cgaaactca tcaaagata 
      aggaggtgg agaatggcg gttcggcgc cacgtattt 
      tatcccttg atgattatt ttatggtcg aacaagaga 
      aagaagtga ctgaagagg gcaaaaaag tgaagatta 
      aagaaatgg tttacgagt acgaggccg aaatggagg 
      agtcctacc ggacttctt tgatcaaat cgttcaaga 
      aaaccataa cagacggtt acttctcgt ttattattg 
      tggacaatg tcaacgaca aagtgaaat atttcggtg 
      aaatgtgga gttatgcga aacagtacg gttttcagg 
      tttacattt gtcagttag atttggacg ttcacactt 
      gttcgaaac gaaatattc acggacgat ctgaggctg 
      acatcgaaa ggtgtgtct caaactggg aacccactc 
      ctcaacatc accccattc tcgacgcca caagtctcc 
      tccaatcag cgtcgattc ccgaagtcg aaatggaag 
      aagttaact cacctgaaa gtgatctga acgaatgtg 
      acgggtcgg aggaccaag tagctagaa gcaagtggg 
      acactttcg actgttctg ttgacaatc tcgcgaaac 
      tcgatggcg tcagcaaac ccttacgac accggacca 
      tggaagaat acctccaat tggatacag agtggactc 
      ccatcaaac ccaccaaac ccgggcaaa agagagtca 
      gatgggccg atttggaag aacagaaac aacaagaga 
      agatgagag caattggat ttgtcgttg gacacacag 
      actgggacc gaatgatgg atccgacag ggggcagtt 
      ctgcgctaa ctcaaacga aatatatcg aacgaacgg 
      gacggttta aagttgatc agaaataaa aacactttt 
      ttgtcattt tttttatta cgagtgaac atttagttg 
      tatgctaat aataaatta aaaatcatg gttttacgg 
      ctttttcgc tagtccgga gtttaaccg gaatttgcg 
      ccatgtatt gattttgcg gcttagttt cttctcttg 
      attccttga caagggtcg agttcaact cactttcgc 
      ccttgcgtt tctgtgctt cgatttgac gcgtctctt 
      gaatttttg cttgcatta cgaccactt gcgggttca 
      tttcgtcca tgtgttctt gtagacagt tttgtagaa 
      tgttgagga agaacaaaa atgcgagga gagttaagg 
      ctgttaaag ctatgctgt gtttgccgc cgccttttg 
      aaccactat tttcacaaa aaaataaaa cgctattaa 
      ttaactaat ttaagtgct tactgactg tgtgatgat 
      ccaccagaa ctgctgtaa tgcgtcctg tcgatgctt 
      agaggcggc ttaacgccc aaatttggc attagcgga 
      ttaggtgtt aacgccatg ttttcttgt acacttgtg 
      cggatttat cccggaact ttgtagtgt cattccttg 
      taactgttt ttccggcct gagattttc agcggaaat 
      aatgtgacg agacgtatc tttgttcca taattgtgc 
      ggcgacaat tttttctct ttgccgatt cattatcca 
      acacctcag acgaaaagg aatagactt ttatgtgtt 
      tggtgtgct gaatgcaat tttattttg aagggatcg 
      tgagaaaat cgcattttg actattttt attggcact 
      atttgtttc agaagtaag tggttctat tgatttttg 
      gtacagaat ttaaatttg aagcccgtt tgtttttat 
      ttaagtact taacaaaaa taataatat gaaatgtga 
      caatacaaa caaatgggt tttaaattt ggattttgg 
      gtgaaaaaa catgtagag actacatgc tttggattt 
      acttaacag aagagtatc aataatgtt caaaatagc 
      cgaaacatg tgatatttt taaaatttg agagtggtt 
      tttaatttc gctgttagc acataaagt gcccctctc 
      gtctaaagg aggagaatt tgttggaca gtgttatta 
      aggccattt gctgtttca taatcagta gtaatacag 
      cccctatag gaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY