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Gene information
General information
TC008355 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8975380..8979565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008355
    iBeetle screen iB_07234

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008355 RA
      MWHEARRQE RKIRGMLVD YRRRAERRR DFYEKIKAD 
      PTQFLQIHG RQCKIHLDP SIAAAADSA SAMMPWQGQ 
      ADNLIDRFD VRAHLDFIP PVKKEEEEE LTFEERQIN 
      YERYRIIAQ NSFLSINEE KFLKQLHLE EQFGYTESK 
      SKKEQRTGG ASIGFNYED NTAQTLAPD YHNDEESDG 
      NSDSDLDVD LSINVSKMD SVQAHEMNR HGRNFGMSS 
      NDFYSFLTN DLEEAESFR LAKEEEHEK ALFSGRKSR 
      RERRAHREK RLANRVISP PSYAARTSP TYPSFRKSK 
      SPSKSPTPE NSGKITYIT SFGDEEESP STSKPNNDK 
      SKQAKKRRR NSSSERTVY RRSYSREKN YKGRRSRSR 
      DTRSRSRAR RRSISRDRY RKSRSRSRG RSYRRKRST 
      SSSSVSSSS TSSSSSKSK SRSSSPKYR KKSSSSRSP 
      SPVKKSKSK SPEKKESPP KVVSRYYGR KKSDQSSSE 
      LSDTSIKDE EMRVANSPV NSEKWTGYG NSGSVIKKT 
      GQNLSIQER LKRKRQALL NKQFKADKI AEQLKTERE 
      KQEQQTREH ELREMAIKL RRRQREKRH AYDSNKSSN 
      SSDSDSSKS KSPPPTVEN REDEPRAIK TRRRQREKR 
      HSSSSSSSD SSKSKSPPP PVPTVSTYN SYKNEKTRS 
      PSPHASRRD DYREARRSR ERRDHSRDR RASRSPRRC 
      NEKKIVYRD RRDRRRDDY ERDRDYKRD SYDRDRDRD 
      RREERGSRS LVDY
    CDS sequence
    • >TC008355 RA
      atgtggcat gaggcaaga cgacaagaa cgcaaaatt 
      cgcggcatg ttggtggac tatcggcga agggctgaa 
      cgacgccgt gatttctac gaaaaaatc aaagccgac 
      cccactcag tttttacaa atccacgga agacaatgc 
      aagattcac ttggacccc tcgatcgca gctgcggca 
      gacagtgct tcggcaatg atgccgtgg caaggacaa 
      gctgataat ttaattgac cgttttgat gtaagagcg 
      catttagat tttatcccc cctgttaag aaggaagaa 
      gaggaggaa ttgacgttt gaagaacgg caaattaat 
      tacgaacgg taccggatt attgcccaa aactcgttc 
      cttagcata aatgaggag aagtttttg aaacaattg 
      catctggag gagcagttt ggctacact gagtctaag 
      tcaaagaag gagcaaagg actggaggt gctagtatt 
      gggtttaac tacgaggac aacactgca caaactttg 
      gctcctgac taccacaat gatgaggaa tcagacggc 
      aattctgat tctgatttg gacgttgat ttgtccatt 
      aatgtttca aaaatggat tcggttcaa gcacacgaa 
      atgaatcgc cacggcaga aactttggg atgagctca 
      aatgatttt tattcgttt cttacaaat gatcttgaa 
      gaggcggaa agcttcaga cttgccaaa gaagaagag 
      catgaaaaa gccttgttt tcaggacga aaatcgcga 
      agggagcgc cgagcgcat agggaaaaa cgtctggct 
      aatagggtg ataagtcct ccaagttac gccgcacgg 
      acatcacct acttaccct tcgtttcgg aaaagtaaa 
      tcgccttca aaatcaccg acgccggaa aatagtggc 
      aaaattaca tacatcaca tcgtttggt gatgaggaa 
      gaaagccct tcgactagt aaaccaaac aatgataag 
      tcaaaacaa gccaagaag agacgaaga aattctagt 
      tcagagaga acagtttat aggagaagt tacagtaga 
      gaaaagaat tataaagga aggaggtca agaagtcga 
      gatacgagg agtcgctca agggcaaga cgccgaagt 
      atctcaaga gatagatac aggaagtcc cggtctcgt 
      tctagaggc cgcagttat cgtcgtaag cgttccact 
      agttcatcg tctgtttct tcatcttcg acttcttca 
      tcttcatct aaaagtaaa tctcgttct tcatcccca 
      aagtatcgc aaaaaatcg tcgtcgtct cgttcgccc 
      agtcctgtg aaaaaatca aagtcaaaa agtcccgag 
      aagaaagaa tctccgccg aaagtcgtt tctcgatat 
      tacggacgg aagaaaagt gatcaaagt tcgtcagaa 
      ttaagcgat acaagcatc aaggacgaa gaaatgaga 
      gtggcaaat agcccggtc aactctgaa aaatggact 
      ggctatggt aattctggc tccgtgatc aaaaaaact 
      ggccagaac ttgagcatt caagagcgc cttaaacgg 
      aaacgccaa gccttatta aataaacaa ttcaaagcc 
      gacaaaatc gcggaacag ttaaagaca gagcgtgaa 
      aaacaggaa cagcagacc agagagcac gagttgcgc 
      gaaatggca ataaaattg cgccgaaga caaagagaa 
      aaacgccac gcttatgat agcaataaa agctcaaat 
      agttctgat tctgattct agtaaaagt aaaagtccg 
      ccgcccaca gttgagaat cgagaagac gaacctcga 
      gcaataaaa acacgaaga agacaaagg gagaaacgt 
      cactcttcg agcagttcc agttccgac tctagcaaa 
      agcaagagt ccgcctcct cctgtccca accgtttca 
      acatacaat tcgtacaaa aatgaaaag acacgaagt 
      ccaagtccg cacgcaagc agaagggat gattatcga 
      gaggcgaga agaagtagg gaaagaaga gatcacagt 
      cgtgataga cgggcttca aggtcgcca agaaggtgc 
      aacgagaaa aaaatcgtg tacagagac agaagggac 
      aggagaaga gatgattat gagcgagac agggactat 
      aaaagagat agttacgat cgagaccgt gatagggat 
      aggagagag gaacgcggt agccgctct ttagtggat 
      tactga
    mRNA sequence
    • >TC008355 RA
      tgtgtgaaa tgtgatggc atttttatt tcttgtaac 
      cgcctctta attattgtt attttcggt tgattacac 
      aagttttaa acataaaaa ccatgtggc atgaggcaa 
      gacgacaag aacgcaaaa ttcgcggca tgttggtgg 
      actatcggc gaagggctg aacgacgcc gtgatttct 
      acgaaaaaa tcaaagccg accccactc agtttttac 
      aaatccacg gaagacaat gcaagattc acttggacc 
      cctcgatcg cagctgcgg cagacagtg cttcggcaa 
      tgatgccgt ggcaaggac aagctgata atttaattg 
      accgttttg atgtaagag cgcatttag attttatcc 
      cccctgtta agaaggaag aagaggagg aattgacgt 
      ttgaagaac ggcaaatta attacgaac ggtaccgga 
      ttattgccc aaaactcgt tccttagca taaatgagg 
      agaagtttt tgaaacaat tgcatctgg aggagcagt 
      ttggctaca ctgagtcta agtcaaaga aggagcaaa 
      ggactggag gtgctagta ttgggttta actacgagg 
      acaacactg cacaaactt tggctcctg actaccaca 
      atgatgagg aatcagacg gcaattctg attctgatt 
      tggacgttg atttgtcca ttaatgttt caaaaatgg 
      attcggttc aagcacacg aaatgaatc gccacggca 
      gaaactttg ggatgagct caaatgatt tttattcgt 
      ttcttacaa atgatcttg aagaggcgg aaagcttca 
      gacttgcca aagaagaag agcatgaaa aagccttgt 
      tttcaggac gaaaatcgc gaagggagc gccgagcgc 
      atagggaaa aacgtctgg ctaataggg tgataagtc 
      ctccaagtt acgccgcac ggacatcac ctacttacc 
      cttcgtttc ggaaaagta aatcgcctt caaaatcac 
      cgacgccgg aaaatagtg gcaaaatta catacatca 
      catcgtttg gtgatgagg aagaaagcc cttcgacta 
      gtaaaccaa acaatgata agtcaaaac aagccaaga 
      agagacgaa gaaattcta gttcagaga gaacagttt 
      ataggagaa gttacagta gagaaaaga attataaag 
      gaaggaggt caagaagtc gagatacga ggagtcgct 
      caagggcaa gacgccgaa gtatctcaa gagatagat 
      acaggaagt cccggtctc gttctagag gccgcagtt 
      atcgtcgta agcgttcca ctagttcat cgtctgttt 
      cttcatctt cgacttctt catcttcat ctaaaagta 
      aatctcgtt cttcatccc caaagtatc gcaaaaaat 
      cgtcgtcgt ctcgttcgc ccagtcctg tgaaaaaat 
      caaagtcaa aaagtcccg agaagaaag aatctccgc 
      cgaaagtcg tttctcgat attacggac ggaagaaaa 
      gtgatcaaa gttcgtcag aattaagcg atacaagca 
      tcaaggacg aagaaatga gagtggcaa atagcccgg 
      tcaactctg aaaaatgga ctggctatg gtaattctg 
      gctccgtga tcaaaaaaa ctggccaga acttgagca 
      ttcaagagc gccttaaac ggaaacgcc aagccttat 
      taaataaac aattcaaag ccgacaaaa tcgcggaac 
      agttaaaga cagagcgtg aaaaacagg aacagcaga 
      ccagagagc acgagttgc gcgaaatgg caataaaat 
      tgcgccgaa gacaaagag aaaaacgcc acgcttatg 
      atagcaata aaagctcaa atagttctg attctgatt 
      ctagtaaaa gtaaaagtc cgccgccca cagttgaga 
      atcgagaag acgaacctc gagcaataa aaacacgaa 
      gaagacaaa gggagaaac gtcactctt cgagcagtt 
      ccagttccg actctagca aaagcaaga gtccgcctc 
      ctcctgtcc caaccgttt caacataca attcgtaca 
      aaaatgaaa agacacgaa gtccaagtc cgcacgcaa 
      gcagaaggg atgattatc gagaggcga gaagaagta 
      gggaaagaa gagatcaca gtcgtgata gacgggctt 
      caaggtcgc caagaaggt gcaacgaga aaaaaatcg 
      tgtacagag acagaaggg acaggagaa gagatgatt 
      atgagcgag acagggact ataaaagag atagttacg 
      atcgagacc gtgataggg ataggagag aggaacgcg 
      gtagccgct ctttagtgg attactgat ttaaattat 
      gataagttt ttgtttgta ataaaattg tttagtaga 
      gctcacacg agtaatttt tttgagggt gaatattta 
      ttagctaag aagtgagaa ataaaggtt gaggtggaa 
      t
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