Status
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Gene information
General information
TC011261 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1129788..1135647
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011261
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07231

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    vitellogenic egg chamber shape irregular - Number of animals showing the phenotype: 6

    previtellogenic egg chamber mostly potentiallynot present - Number of animals showing the phenotype: 6

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011261 RA
      MLKIGQTVL NNLKTGKNL NVSRLLKST IAEAKVSEI 
      QEEPKPKKY ERSQNNMVA AVFASLQNE VKTPLTDDK 
      ISKASDINE LLSISEGSG VSRKHALKV VSTLAEWSS 
      SGKTQLSEF ENDHRFIKL CRILSKGAK VNRAMVSRS 
      EDLSTVLSV TADDEAARL INNLTLPQM IKVMSTLTM 
      RKRRSVLLL RTLAYNIAA SGETLNLKQ SADLLYSIS 
      VDIFLLDLS EVLDSLSEW ILQNHLICR PQDVFSLFV 
      TLATVNYLP TNSEELFKV LVPQLTISE ARKPLVWLD 
      FVWSLVILN QASQSHISS VLAPDFVEK IEDASVFGK 
      VKLLNINGA AKYLMEGFT GETLSPLSE IHNVNIVRT 
      KENGEMAEC VLDTLKNLI QSDNLNFDV NTGLGFSID 
      VECLLDKKC NPLPITKEN SNKTDFTKI ALIAYDYHN 
      MCIGKVEPT GANVLNSRL LEACGYKVL AIPYTEFKI 
      TDKLVHRVQ YLESKLKNI VK
    • >TC011261 RB
      MLKIGQTVL NNLKTGKNL NVSRLLKST IAEAKVSEI 
      QEEPKPKKY ERSQNNMVA AVFASLQNE VKTPLTDDK 
      ISKASDINE LLSISEGSG VSRKHALKV VSTLAEWSS 
      SGKTQLSEF ENDHRFIKL CRILSKGAK VNRAMVSRS 
      EDLSTVLSV TADDEAARL INNLTLPQM IKVMSTLTM 
      RKRRSVLLL RTLAYNIAA SGETLNLKQ SADLLYSIS 
      VDIFLLDLS EVLDSLSEW ILQNHLICR PQDVFSLFV 
      TLATVNYLP TNSEELFKV LVPQLTISE ARKPLVWLD 
      FVWSLVILN QASQSHISS VLAPDFVEK IEVDASVFG 
      KVKLLNING AAKYLMEGF TGETLSPLS EIHNVNIVR 
      TKENGEMAE CVLDTLKNL IQSDNLNFD VNTGLGFSI 
      DVECLLDKK CNPLPITKE NSNKTDFTK IALIAYDYH 
      NMCIGKVEP TGANVLNSR LLEACGYKV LAIPYTEFK 
      ITDKLVHRV QYLESKLKN IVK
    CDS sequence
    • >TC011261 RA
      atgcttaaa ataggccaa accgtacta aacaacctc 
      aaaaccggc aaaaacctg aacgtgtcc cgattgcta 
      aaatcaacc atagcagag gcaaaagtg agcgaaatc 
      caggaagaa ccaaagcca aaaaaatat gagaggtcg 
      caaaataac atggttgcg gctgtgttt gcgtccctt 
      caaaacgaa gtcaaaact ccactgacc gatgataaa 
      ataagcaaa gccagtgac attaacgaa ctgctttca 
      atatcagag ggtagcgga gtctcgcga aaacatgcc 
      ctgaaggtc gtttcgacc ctggccgaa tggtcatca 
      tcaggaaaa actcagtta tcggagttt gaaaatgat 
      catcgcttt atcaaactg tgtcgaatt ttgagcaag 
      ggggccaaa gtcaacaga gctatggtg tcaaggagt 
      gaagatttg tcgactgtg cttagtgtc acagctgat 
      gatgaggcc gcaaggctt ataaataat ttaacgttg 
      ccgcaaatg atcaaggtg atgtcaaca ctcacgatg 
      agaaaacgt cgatcagtg cttttgtta cgcacgctt 
      gcctataat atagcagcc agtggggag acacttaat 
      ttaaaacag agcgcagac ttattatac agcatcagt 
      gttgacata tttctgttg gatctatcg gaagttttg 
      gattcactg agtgaatgg attttgcaa aaccatttg 
      atttgccga cctcaggat gttttcagc ttatttgtg 
      actttggct acagtcaac tatttgcca accaattct 
      gaggaactg ttcaaagtg ttggtgcca caattaaca 
      atttcagaa gcaaggaaa cctctggtg tggttggac 
      tttgtttgg tcactcgtt attttaaat caggcctct 
      cagagtcat atttctagc gtcctagcg ccggatttt 
      gttgaaaaa attgaagat gcgagtgtg tttgggaaa 
      gtaaaactg ttaaatata aacggtgcc gctaaatat 
      ttgatggaa ggttttacc ggtgagaca ttgtcgcct 
      ttgtcagaa atccacaac gtgaatatt gttaggacg 
      aaggaaaac ggagaaatg gctgagtgt gttttagac 
      actttgaag aacttgatt cagtcagac aacttaaat 
      tttgatgtg aacacagga ctggggttt tccatagat 
      gttgagtgt ttacttgac aaaaaatgc aatccactt 
      ccaatcact aaagaaaat tccaacaaa actgatttc 
      accaagatt gctttaata gcatacgat taccataat 
      atgtgtata ggcaaagtg gaacctact ggtgctaat 
      gtcctcaat tctcgacta cttgaagct tgtggttat 
      aaggtacta gctattcct tacacggaa tttaaaatc 
      acggacaag ttggtgcat cgagtccag tatctagaa 
      agcaagcta aaaaatatt gtcaagtga
    • >TC011261 RB
      atgcttaaa ataggccaa accgtacta aacaacctc 
      aaaaccggc aaaaacctg aacgtgtcc cgattgcta 
      aaatcaacc atagcagag gcaaaagtg agcgaaatc 
      caggaagaa ccaaagcca aaaaaatat gagaggtcg 
      caaaataac atggttgcg gctgtgttt gcgtccctt 
      caaaacgaa gtcaaaact ccactgacc gatgataaa 
      ataagcaaa gccagtgac attaacgaa ctgctttca 
      atatcagag ggtagcgga gtctcgcga aaacatgcc 
      ctgaaggtc gtttcgacc ctggccgaa tggtcatca 
      tcaggaaaa actcagtta tcggagttt gaaaatgat 
      catcgcttt atcaaactg tgtcgaatt ttgagcaag 
      ggggccaaa gtcaacaga gctatggtg tcaaggagt 
      gaagatttg tcgactgtg cttagtgtc acagctgat 
      gatgaggcc gcaaggctt ataaataat ttaacgttg 
      ccgcaaatg atcaaggtg atgtcaaca ctcacgatg 
      agaaaacgt cgatcagtg cttttgtta cgcacgctt 
      gcctataat atagcagcc agtggggag acacttaat 
      ttaaaacag agcgcagac ttattatac agcatcagt 
      gttgacata tttctgttg gatctatcg gaagttttg 
      gattcactg agtgaatgg attttgcaa aaccatttg 
      atttgccga cctcaggat gttttcagc ttatttgtg 
      actttggct acagtcaac tatttgcca accaattct 
      gaggaactg ttcaaagtg ttggtgcca caattaaca 
      atttcagaa gcaaggaaa cctctggtg tggttggac 
      tttgtttgg tcactcgtt attttaaat caggcctct 
      cagagtcat atttctagc gtcctagcg ccggatttt 
      gttgaaaaa attgaagta gatgcgagt gtgtttggg 
      aaagtaaaa ctgttaaat ataaacggt gccgctaaa 
      tatttgatg gaaggtttt accggtgag acattgtcg 
      cctttgtca gaaatccac aacgtgaat attgttagg 
      acgaaggaa aacggagaa atggctgag tgtgtttta 
      gacactttg aagaacttg attcagtca gacaactta 
      aattttgat gtgaacaca ggactgggg ttttccata 
      gatgttgag tgtttactt gacaaaaaa tgcaatcca 
      cttccaatc actaaagaa aattccaac aaaactgat 
      ttcaccaag attgcttta atagcatac gattaccat 
      aatatgtgt ataggcaaa gtggaacct actggtgct 
      aatgtcctc aattctcga ctacttgaa gcttgtggt 
      tataaggta ctagctatt ccttacacg gaatttaaa 
      atcacggac aagttggtg catcgagtc cagtatcta 
      gaaagcaag ctaaaaaat attgtcaag tga
    mRNA sequence
    • >TC011261 RA
      tccttgtca aaatttgtg caaaagtta ctaaaaagt 
      gaggttaga gattttaag atttagggg tgtttttta 
      attaatagt gcattttac cgcaaagtt ttgagtgaa 
      aacataaca attacaaat cctggaaga taattaaag 
      tgctgtttc ttgttgata accccgtgt aaccccatg 
      gaatgaagt gaggttagg acatcacat acctattat 
      gtcataaga gggaccgtc taaaaacag tcatatttt 
      tggtaataa agaggcaaa atgcttaaa ataggccaa 
      accgtacta aacaacctc aaaaccggc aaaaacctg 
      aacgtgtcc cgattgcta aaatcaacc atagcagag 
      gcaaaagtg agcgaaatc caggaagaa ccaaagcca 
      aaaaaatat gagaggtcg caaaataac atggttgcg 
      gctgtgttt gcgtccctt caaaacgaa gtcaaaact 
      ccactgacc gatgataaa ataagcaaa gccagtgac 
      attaacgaa ctgctttca atatcagag ggtagcgga 
      gtctcgcga aaacatgcc ctgaaggtc gtttcgacc 
      ctggccgaa tggtcatca tcaggaaaa actcagtta 
      tcggagttt gaaaatgat catcgcttt atcaaactg 
      tgtcgaatt ttgagcaag ggggccaaa gtcaacaga 
      gctatggtg tcaaggagt gaagatttg tcgactgtg 
      cttagtgtc acagctgat gatgaggcc gcaaggctt 
      ataaataat ttaacgttg ccgcaaatg atcaaggtg 
      atgtcaaca ctcacgatg agaaaacgt cgatcagtg 
      cttttgtta cgcacgctt gcctataat atagcagcc 
      agtggggag acacttaat ttaaaacag agcgcagac 
      ttattatac agcatcagt gttgacata tttctgttg 
      gatctatcg gaagttttg gattcactg agtgaatgg 
      attttgcaa aaccatttg atttgccga cctcaggat 
      gttttcagc ttatttgtg actttggct acagtcaac 
      tatttgcca accaattct gaggaactg ttcaaagtg 
      ttggtgcca caattaaca atttcagaa gcaaggaaa 
      cctctggtg tggttggac tttgtttgg tcactcgtt 
      attttaaat caggcctct cagagtcat atttctagc 
      gtcctagcg ccggatttt gttgaaaaa attgaagat 
      gcgagtgtg tttgggaaa gtaaaactg ttaaatata 
      aacggtgcc gctaaatat ttgatggaa ggttttacc 
      ggtgagaca ttgtcgcct ttgtcagaa atccacaac 
      gtgaatatt gttaggacg aaggaaaac ggagaaatg 
      gctgagtgt gttttagac actttgaag aacttgatt 
      cagtcagac aacttaaat tttgatgtg aacacagga 
      ctggggttt tccatagat gttgagtgt ttacttgac 
      aaaaaatgc aatccactt ccaatcact aaagaaaat 
      tccaacaaa actgatttc accaagatt gctttaata 
      gcatacgat taccataat atgtgtata ggcaaagtg 
      gaacctact ggtgctaat gtcctcaat tctcgacta 
      cttgaagct tgtggttat aaggtacta gctattcct 
      tacacggaa tttaaaatc acggacaag ttggtgcat 
      cgagtccag tatctagaa agcaagcta aaaaatatt 
      gtcaagtga agaatgtaa tttattata gataaataa 
      agagacttt ttgagt
    • >TC011261 RB
      tccttgtca aaatttgtg caaaagtta ctaaaaagt 
      gaggttaga gattttaag atttagggg tgtttttta 
      attaatagt gcattttac cgcaaagtt ttgagtgaa 
      aacataaca attacaaat cctggaaga taattaaag 
      tgctgtttc ttgttgata accccgtgt aaccccatg 
      gaatgaagt gaggttagg acatcacat acctattat 
      gtcataaga gggaccgtc taaaaacag tcatatttt 
      tggtaataa agaggcaaa atgcttaaa ataggccaa 
      accgtacta aacaacctc aaaaccggc aaaaacctg 
      aacgtgtcc cgattgcta aaatcaacc atagcagag 
      gcaaaagtg agcgaaatc caggaagaa ccaaagcca 
      aaaaaatat gagaggtcg caaaataac atggttgcg 
      gctgtgttt gcgtccctt caaaacgaa gtcaaaact 
      ccactgacc gatgataaa ataagcaaa gccagtgac 
      attaacgaa ctgctttca atatcagag ggtagcgga 
      gtctcgcga aaacatgcc ctgaaggtc gtttcgacc 
      ctggccgaa tggtcatca tcaggaaaa actcagtta 
      tcggagttt gaaaatgat catcgcttt atcaaactg 
      tgtcgaatt ttgagcaag ggggccaaa gtcaacaga 
      gctatggtg tcaaggagt gaagatttg tcgactgtg 
      cttagtgtc acagctgat gatgaggcc gcaaggctt 
      ataaataat ttaacgttg ccgcaaatg atcaaggtg 
      atgtcaaca ctcacgatg agaaaacgt cgatcagtg 
      cttttgtta cgcacgctt gcctataat atagcagcc 
      agtggggag acacttaat ttaaaacag agcgcagac 
      ttattatac agcatcagt gttgacata tttctgttg 
      gatctatcg gaagttttg gattcactg agtgaatgg 
      attttgcaa aaccatttg atttgccga cctcaggat 
      gttttcagc ttatttgtg actttggct acagtcaac 
      tatttgcca accaattct gaggaactg ttcaaagtg 
      ttggtgcca caattaaca atttcagaa gcaaggaaa 
      cctctggtg tggttggac tttgtttgg tcactcgtt 
      attttaaat caggcctct cagagtcat atttctagc 
      gtcctagcg ccggatttt gttgaaaaa attgaagta 
      gatgcgagt gtgtttggg aaagtaaaa ctgttaaat 
      ataaacggt gccgctaaa tatttgatg gaaggtttt 
      accggtgag acattgtcg cctttgtca gaaatccac 
      aacgtgaat attgttagg acgaaggaa aacggagaa 
      atggctgag tgtgtttta gacactttg aagaacttg 
      attcagtca gacaactta aattttgat gtgaacaca 
      ggactgggg ttttccata gatgttgag tgtttactt 
      gacaaaaaa tgcaatcca cttccaatc actaaagaa 
      aattccaac aaaactgat ttcaccaag attgcttta 
      atagcatac gattaccat aatatgtgt ataggcaaa 
      gtggaacct actggtgct aatgtcctc aattctcga 
      ctacttgaa gcttgtggt tataaggta ctagctatt 
      ccttacacg gaatttaaa atcacggac aagttggtg 
      catcgagtc cagtatcta gaaagcaag ctaaaaaat 
      attgtcaag tgaagaatg taatttatt atagataaa 
      taaagagac tttttgagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY