Status
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Gene information
General information
TC011261 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1129788..1135647
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011261
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07231

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    vitellogenic egg chamber shape irregular - Number of animals showing the phenotype: 6

    previtellogenic egg chamber mostly potentiallynot present - Number of animals showing the phenotype: 6

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011261 RA
      MLKIGQTVL NNLKTGKNL NVSRLLKST IAEAKVSEI 
QEEPKPKKY ERSQNNMVA AVFASLQNE VKTPLTDDK 
ISKASDINE LLSISEGSG VSRKHALKV VSTLAEWSS 
SGKTQLSEF ENDHRFIKL CRILSKGAK VNRAMVSRS 
EDLSTVLSV TADDEAARL INNLTLPQM IKVMSTLTM 
RKRRSVLLL RTLAYNIAA SGETLNLKQ SADLLYSIS 
VDIFLLDLS EVLDSLSEW ILQNHLICR PQDVFSLFV 
TLATVNYLP TNSEELFKV LVPQLTISE ARKPLVWLD 
FVWSLVILN QASQSHISS VLAPDFVEK IEDASVFGK 
VKLLNINGA AKYLMEGFT GETLSPLSE IHNVNIVRT 
KENGEMAEC VLDTLKNLI QSDNLNFDV NTGLGFSID 
VECLLDKKC NPLPITKEN SNKTDFTKI ALIAYDYHN 
MCIGKVEPT GANVLNSRL LEACGYKVL AIPYTEFKI 
TDKLVHRVQ YLESKLKNI VK
    • >TC011261 RB
      MLKIGQTVL NNLKTGKNL NVSRLLKST IAEAKVSEI 
QEEPKPKKY ERSQNNMVA AVFASLQNE VKTPLTDDK 
ISKASDINE LLSISEGSG VSRKHALKV VSTLAEWSS 
SGKTQLSEF ENDHRFIKL CRILSKGAK VNRAMVSRS 
EDLSTVLSV TADDEAARL INNLTLPQM IKVMSTLTM 
RKRRSVLLL RTLAYNIAA SGETLNLKQ SADLLYSIS 
VDIFLLDLS EVLDSLSEW ILQNHLICR PQDVFSLFV 
TLATVNYLP TNSEELFKV LVPQLTISE ARKPLVWLD 
FVWSLVILN QASQSHISS VLAPDFVEK IEVDASVFG 
KVKLLNING AAKYLMEGF TGETLSPLS EIHNVNIVR 
TKENGEMAE CVLDTLKNL IQSDNLNFD VNTGLGFSI 
DVECLLDKK CNPLPITKE NSNKTDFTK IALIAYDYH 
NMCIGKVEP TGANVLNSR LLEACGYKV LAIPYTEFK 
ITDKLVHRV QYLESKLKN IVK
    CDS sequence
    • >TC011261 RA
      atgcttaaa ataggccaa accgtacta aacaacctc 
aaaaccggc aaaaacctg aacgtgtcc cgattgcta 
aaatcaacc atagcagag gcaaaagtg agcgaaatc 
caggaagaa ccaaagcca aaaaaatat gagaggtcg 
caaaataac atggttgcg gctgtgttt gcgtccctt 
caaaacgaa gtcaaaact ccactgacc gatgataaa 
ataagcaaa gccagtgac attaacgaa ctgctttca 
atatcagag ggtagcgga gtctcgcga aaacatgcc 
ctgaaggtc gtttcgacc ctggccgaa tggtcatca 
tcaggaaaa actcagtta tcggagttt gaaaatgat 
catcgcttt atcaaactg tgtcgaatt ttgagcaag 
ggggccaaa gtcaacaga gctatggtg tcaaggagt 
gaagatttg tcgactgtg cttagtgtc acagctgat 
gatgaggcc gcaaggctt ataaataat ttaacgttg 
ccgcaaatg atcaaggtg atgtcaaca ctcacgatg 
agaaaacgt cgatcagtg cttttgtta cgcacgctt 
gcctataat atagcagcc agtggggag acacttaat 
ttaaaacag agcgcagac ttattatac agcatcagt 
gttgacata tttctgttg gatctatcg gaagttttg 
gattcactg agtgaatgg attttgcaa aaccatttg 
atttgccga cctcaggat gttttcagc ttatttgtg 
actttggct acagtcaac tatttgcca accaattct 
gaggaactg ttcaaagtg ttggtgcca caattaaca 
atttcagaa gcaaggaaa cctctggtg tggttggac 
tttgtttgg tcactcgtt attttaaat caggcctct 
cagagtcat atttctagc gtcctagcg ccggatttt 
gttgaaaaa attgaagat gcgagtgtg tttgggaaa 
gtaaaactg ttaaatata aacggtgcc gctaaatat 
ttgatggaa ggttttacc ggtgagaca ttgtcgcct 
ttgtcagaa atccacaac gtgaatatt gttaggacg 
aaggaaaac ggagaaatg gctgagtgt gttttagac 
actttgaag aacttgatt cagtcagac aacttaaat 
tttgatgtg aacacagga ctggggttt tccatagat 
gttgagtgt ttacttgac aaaaaatgc aatccactt 
ccaatcact aaagaaaat tccaacaaa actgatttc 
accaagatt gctttaata gcatacgat taccataat 
atgtgtata ggcaaagtg gaacctact ggtgctaat 
gtcctcaat tctcgacta cttgaagct tgtggttat 
aaggtacta gctattcct tacacggaa tttaaaatc 
acggacaag ttggtgcat cgagtccag tatctagaa 
agcaagcta aaaaatatt gtcaagtga
    • >TC011261 RB
      atgcttaaa ataggccaa accgtacta aacaacctc 
aaaaccggc aaaaacctg aacgtgtcc cgattgcta 
aaatcaacc atagcagag gcaaaagtg agcgaaatc 
caggaagaa ccaaagcca aaaaaatat gagaggtcg 
caaaataac atggttgcg gctgtgttt gcgtccctt 
caaaacgaa gtcaaaact ccactgacc gatgataaa 
ataagcaaa gccagtgac attaacgaa ctgctttca 
atatcagag ggtagcgga gtctcgcga aaacatgcc 
ctgaaggtc gtttcgacc ctggccgaa tggtcatca 
tcaggaaaa actcagtta tcggagttt gaaaatgat 
catcgcttt atcaaactg tgtcgaatt ttgagcaag 
ggggccaaa gtcaacaga gctatggtg tcaaggagt 
gaagatttg tcgactgtg cttagtgtc acagctgat 
gatgaggcc gcaaggctt ataaataat ttaacgttg 
ccgcaaatg atcaaggtg atgtcaaca ctcacgatg 
agaaaacgt cgatcagtg cttttgtta cgcacgctt 
gcctataat atagcagcc agtggggag acacttaat 
ttaaaacag agcgcagac ttattatac agcatcagt 
gttgacata tttctgttg gatctatcg gaagttttg 
gattcactg agtgaatgg attttgcaa aaccatttg 
atttgccga cctcaggat gttttcagc ttatttgtg 
actttggct acagtcaac tatttgcca accaattct 
gaggaactg ttcaaagtg ttggtgcca caattaaca 
atttcagaa gcaaggaaa cctctggtg tggttggac 
tttgtttgg tcactcgtt attttaaat caggcctct 
cagagtcat atttctagc gtcctagcg ccggatttt 
gttgaaaaa attgaagta gatgcgagt gtgtttggg 
aaagtaaaa ctgttaaat ataaacggt gccgctaaa 
tatttgatg gaaggtttt accggtgag acattgtcg 
cctttgtca gaaatccac aacgtgaat attgttagg 
acgaaggaa aacggagaa atggctgag tgtgtttta 
gacactttg aagaacttg attcagtca gacaactta 
aattttgat gtgaacaca ggactgggg ttttccata 
gatgttgag tgtttactt gacaaaaaa tgcaatcca 
cttccaatc actaaagaa aattccaac aaaactgat 
ttcaccaag attgcttta atagcatac gattaccat 
aatatgtgt ataggcaaa gtggaacct actggtgct 
aatgtcctc aattctcga ctacttgaa gcttgtggt 
tataaggta ctagctatt ccttacacg gaatttaaa 
atcacggac aagttggtg catcgagtc cagtatcta 
gaaagcaag ctaaaaaat attgtcaag tga
    mRNA sequence
    • >TC011261 RA
      tccttgtca aaatttgtg caaaagtta ctaaaaagt 
gaggttaga gattttaag atttagggg tgtttttta 
attaatagt gcattttac cgcaaagtt ttgagtgaa 
aacataaca attacaaat cctggaaga taattaaag 
tgctgtttc ttgttgata accccgtgt aaccccatg 
gaatgaagt gaggttagg acatcacat acctattat 
gtcataaga gggaccgtc taaaaacag tcatatttt 
tggtaataa agaggcaaa atgcttaaa ataggccaa 
accgtacta aacaacctc aaaaccggc aaaaacctg 
aacgtgtcc cgattgcta aaatcaacc atagcagag 
gcaaaagtg agcgaaatc caggaagaa ccaaagcca 
aaaaaatat gagaggtcg caaaataac atggttgcg 
gctgtgttt gcgtccctt caaaacgaa gtcaaaact 
ccactgacc gatgataaa ataagcaaa gccagtgac 
attaacgaa ctgctttca atatcagag ggtagcgga 
gtctcgcga aaacatgcc ctgaaggtc gtttcgacc 
ctggccgaa tggtcatca tcaggaaaa actcagtta 
tcggagttt gaaaatgat catcgcttt atcaaactg 
tgtcgaatt ttgagcaag ggggccaaa gtcaacaga 
gctatggtg tcaaggagt gaagatttg tcgactgtg 
cttagtgtc acagctgat gatgaggcc gcaaggctt 
ataaataat ttaacgttg ccgcaaatg atcaaggtg 
atgtcaaca ctcacgatg agaaaacgt cgatcagtg 
cttttgtta cgcacgctt gcctataat atagcagcc 
agtggggag acacttaat ttaaaacag agcgcagac 
ttattatac agcatcagt gttgacata tttctgttg 
gatctatcg gaagttttg gattcactg agtgaatgg 
attttgcaa aaccatttg atttgccga cctcaggat 
gttttcagc ttatttgtg actttggct acagtcaac 
tatttgcca accaattct gaggaactg ttcaaagtg 
ttggtgcca caattaaca atttcagaa gcaaggaaa 
cctctggtg tggttggac tttgtttgg tcactcgtt 
attttaaat caggcctct cagagtcat atttctagc 
gtcctagcg ccggatttt gttgaaaaa attgaagat 
gcgagtgtg tttgggaaa gtaaaactg ttaaatata 
aacggtgcc gctaaatat ttgatggaa ggttttacc 
ggtgagaca ttgtcgcct ttgtcagaa atccacaac 
gtgaatatt gttaggacg aaggaaaac ggagaaatg 
gctgagtgt gttttagac actttgaag aacttgatt 
cagtcagac aacttaaat tttgatgtg aacacagga 
ctggggttt tccatagat gttgagtgt ttacttgac 
aaaaaatgc aatccactt ccaatcact aaagaaaat 
tccaacaaa actgatttc accaagatt gctttaata 
gcatacgat taccataat atgtgtata ggcaaagtg 
gaacctact ggtgctaat gtcctcaat tctcgacta 
cttgaagct tgtggttat aaggtacta gctattcct 
tacacggaa tttaaaatc acggacaag ttggtgcat 
cgagtccag tatctagaa agcaagcta aaaaatatt 
gtcaagtga agaatgtaa tttattata gataaataa 
agagacttt ttgagt
    • >TC011261 RB
      tccttgtca aaatttgtg caaaagtta ctaaaaagt 
gaggttaga gattttaag atttagggg tgtttttta 
attaatagt gcattttac cgcaaagtt ttgagtgaa 
aacataaca attacaaat cctggaaga taattaaag 
tgctgtttc ttgttgata accccgtgt aaccccatg 
gaatgaagt gaggttagg acatcacat acctattat 
gtcataaga gggaccgtc taaaaacag tcatatttt 
tggtaataa agaggcaaa atgcttaaa ataggccaa 
accgtacta aacaacctc aaaaccggc aaaaacctg 
aacgtgtcc cgattgcta aaatcaacc atagcagag 
gcaaaagtg agcgaaatc caggaagaa ccaaagcca 
aaaaaatat gagaggtcg caaaataac atggttgcg 
gctgtgttt gcgtccctt caaaacgaa gtcaaaact 
ccactgacc gatgataaa ataagcaaa gccagtgac 
attaacgaa ctgctttca atatcagag ggtagcgga 
gtctcgcga aaacatgcc ctgaaggtc gtttcgacc 
ctggccgaa tggtcatca tcaggaaaa actcagtta 
tcggagttt gaaaatgat catcgcttt atcaaactg 
tgtcgaatt ttgagcaag ggggccaaa gtcaacaga 
gctatggtg tcaaggagt gaagatttg tcgactgtg 
cttagtgtc acagctgat gatgaggcc gcaaggctt 
ataaataat ttaacgttg ccgcaaatg atcaaggtg 
atgtcaaca ctcacgatg agaaaacgt cgatcagtg 
cttttgtta cgcacgctt gcctataat atagcagcc 
agtggggag acacttaat ttaaaacag agcgcagac 
ttattatac agcatcagt gttgacata tttctgttg 
gatctatcg gaagttttg gattcactg agtgaatgg 
attttgcaa aaccatttg atttgccga cctcaggat 
gttttcagc ttatttgtg actttggct acagtcaac 
tatttgcca accaattct gaggaactg ttcaaagtg 
ttggtgcca caattaaca atttcagaa gcaaggaaa 
cctctggtg tggttggac tttgtttgg tcactcgtt 
attttaaat caggcctct cagagtcat atttctagc 
gtcctagcg ccggatttt gttgaaaaa attgaagta 
gatgcgagt gtgtttggg aaagtaaaa ctgttaaat 
ataaacggt gccgctaaa tatttgatg gaaggtttt 
accggtgag acattgtcg cctttgtca gaaatccac 
aacgtgaat attgttagg acgaaggaa aacggagaa 
atggctgag tgtgtttta gacactttg aagaacttg 
attcagtca gacaactta aattttgat gtgaacaca 
ggactgggg ttttccata gatgttgag tgtttactt 
gacaaaaaa tgcaatcca cttccaatc actaaagaa 
aattccaac aaaactgat ttcaccaag attgcttta 
atagcatac gattaccat aatatgtgt ataggcaaa 
gtggaacct actggtgct aatgtcctc aattctcga 
ctacttgaa gcttgtggt tataaggta ctagctatt 
ccttacacg gaatttaaa atcacggac aagttggtg 
catcgagtc cagtatcta gaaagcaag ctaaaaaat 
attgtcaag tgaagaatg taatttatt atagataaa 
taaagagac tttttgagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY