Status
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Gene information
General information
TC008315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8404742..8410192
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008315
    iBeetle screen iB_07227

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008315 RA
      MEEGAGDAV TELLSGVDF ENTKHQKVI SNGYGPYSG 
      GYANGYTCI NKRNGYRGS EQNGHVLAS SDEECTAKN 
      MKGPIKNGY VKTTLKEPL KTRILKESF EPPTLLTAA 
      LTHISFYIL MFLGYLSQA LFPPKIVKE KNRDGYLPL 
      FDRFASFYS RYVYRRIRD CWNYPICSV PGNEVVLKD 
      RVTNDNGWT FEFTGSKTK CLNLASYNY LGFAENSGP 
      CAEFAIDAI YKYGVTTGG TRQQYGTCD LHVELEKLT 
      AEFLGVEDA ITFGMGFAT NALNIPTLL SPGCLVISD 
      EKNHASLIL GIRLPGATV KVFKHNDVK HLEQILRES 
      IYKGQPSQE GYKPWKKIL IVVEGVYSM EGTIVRLPE 
      IIALKKKYK AYLYLDEAH SIGAMGKHG RGIVDYFGC 
      DPKDIDILM GTFTKSFGS AGGYIAGTK ELIDFLRKN 
      SFASKYAWA MAPPVAAQI IAVLKILMG RDGTNEGQK 
      RIETLARNT RYFRRRIEQ MGIIIHGNE DSPVVPILV 
      YLYSKIAAM VRTLIKEKI ATVGVGYPA TPLMEGRIR 
      ICLSAAHTK EQLDYALAV IEKVADEIG LKYSRKPID 
      PTPIDYDKI KVYVD
    • >TC008315 RB
      MQGCLTVSR ESQSTNKKF AFVKCGRMR PQILSIEAR 
      MEEGAGDAV TELLSGVDF ENTKHQKVI SNGYGPYSG 
      GYANGYTCI NKRNGYRGS EQNGHVLAS SDEECTAKN 
      MKGPIKNGY VKTTLKEPL KTRILKESF EPPTLLTAA 
      LTHISFYIL MFLGYLSQA LFPPKIVKE KNRDGYLPL 
      FDRFASFYS RYVYRRIRD CWNYPICSV PGNEVVLKD 
      RVTNDNGWT FEFTGSKTK CLNLASYNY LGFAENSGP 
      CAEFAIDAI YKYGVTTGG TRQQYGTCD LHVELEKLT 
      AEFLGVEDA ITFGMGFAT NALNIPTLL SPGCLVISD 
      EKNHASLIL GIRLPGATV KVFKHNDVK HLEQILRES 
      IYKGQPSQE GYKPWKKIL IVVEGVYSM EGTIVRLPE 
      IIALKKKYK AYLYLDEAH SIGAMGKHG RGIVDYFGC 
      DPKDIDILM GTFTKSFGS AGGYIAGTK ELIDFLRKN 
      SFASKYAWA MAPPVAAQI IAVLKILMG RDGTNEGQK 
      RIETLARNT RYFRRRIEQ MGIIIHGNE DSPVVPILV 
      YLYSKIAAM VRTLIKEKI ATVGVGYPA TPLMEGRIR 
      ICLSAAHTK EQLDYALAV IEKVADEIG LKYSRKPID 
      PTPIDYDKI KVYVD
    CDS sequence
    • >TC008315 RA
      atggaagag ggtgcaggt gatgctgtg acagagcta 
      ctaagtggg gtagatttc gaaaatacc aaacaccaa 
      aaggtcatt tcgaacgga tacggcccc tactcggga 
      ggctacgca aatggatat acgtgcatc aataagagg 
      aacgggtac agaggatcc gagcagaat ggacatgtg 
      ctggcatcg tctgacgag gaatgcacc gccaaaaac 
      atgaaggga ccaataaaa aacggttat gtaaaaaca 
      actttaaag gaaccgcta aaaacccga atattgaaa 
      gaatccttc gaaccaccg actctcctc acagcggct 
      ttaacgcac attagtttc tatatttta atgtttctc 
      ggttatttg agccaggcc ttgtttcca ccaaagata 
      gtcaaagag aaaaatcgg gacggttat ctccctctg 
      tttgataga tttgcaagt ttttactcg cgttatgtc 
      taccgaaga ataagggat tgctggaac taccccatt 
      tgcagcgtt ccggggaac gaagtggtt ttgaaagac 
      agggtcacg aacgacaac ggatggacc tttgagttt 
      accggctct aaaaccaaa tgcttgaac ttggcctcg 
      tacaattac ctcggtttt gcggaaaat tccggccct 
      tgtgccgag tttgcaatt gacgcgatt tacaaatac 
      ggggtcacc acaggtggt actagacaa cagtacggc 
      acttgtgat ttgcacgtc gaattggaa aaattaact 
      gcggagttt ttgggcgtc gaagacgca attaccttc 
      ggaatgggt tttgcaaca aacgccctc aatattccg 
      acgctgtta tcacccggt tgtctcgtt atcagtgac 
      gagaaaaac cacgcttcg ctcattttg gggattcgt 
      ttaccgggg gccactgtc aaagttttc aaacataac 
      gatgtgaag catctggag caaattttg cgcgagagc 
      atctacaag ggccagccg agccaagag gggtacaag 
      ccgtggaag aaaattttg atcgtggtc gaaggtgtc 
      tacagtatg gagggcaca atcgtccgt ttacctgaa 
      ataatagca cttaaaaag aagtacaaa gcttattta 
      tacctagat gaagcgcat agcatcggt gcgatgggc 
      aagcacggc cgaggcatc gttgattat ttcgggtgc 
      gaccccaaa gatatagat attttgatg gggactttc 
      accaagagt tttggctcc gctgggggc tacatagcc 
      ggaaccaaa gaattgatc gattttttg cggaagaat 
      agttttgct tcgaaatac gcctgggcc atggcgcca 
      cctgtagcc gcacaaatt atcgcagtt ttgaaaata 
      ctgatggga cgggatggt actaatgag ggacagaaa 
      aggatcgaa acgctggca cgcaatacg aggtatttc 
      aggaggagg atcgaacag atggggatt attatacac 
      gggaatgag gattcgccg gttgttccg atccttgtc 
      tacctctac tcgaaaatt gcagctatg gtcaggacg 
      ctgattaag gagaaaatc gcaacggtt ggtgtcgga 
      tatccggcg acgccgttg atggaaggc cggataagg 
      atctgtctg tcagccgca cacaccaaa gaacagctc 
      gactacgcc ttggctgtt atcgaaaaa gttgcagat 
      gagataggg ttgaaatat tcgcgcaag ccgattgat 
      ccaacacct atcgattat gacaaaatc aaggtgtat 
      gtcgactaa
    • >TC008315 RB
      atgcaaggt tgtctcacg gtatcacgc gagtcgcaa 
      tcaaccaac aaaaaattt gcgtttgta aaatgtgga 
      cgcatgcgt cctcaaatc ctcagtata gaagcgaga 
      atggaagag ggtgcaggt gatgctgtg acagagcta 
      ctaagtggg gtagatttc gaaaatacc aaacaccaa 
      aaggtcatt tcgaacgga tacggcccc tactcggga 
      ggctacgca aatggatat acgtgcatc aataagagg 
      aacgggtac agaggatcc gagcagaat ggacatgtg 
      ctggcatcg tctgacgag gaatgcacc gccaaaaac 
      atgaaggga ccaataaaa aacggttat gtaaaaaca 
      actttaaag gaaccgcta aaaacccga atattgaaa 
      gaatccttc gaaccaccg actctcctc acagcggct 
      ttaacgcac attagtttc tatatttta atgtttctc 
      ggttatttg agccaggcc ttgtttcca ccaaagata 
      gtcaaagag aaaaatcgg gacggttat ctccctctg 
      tttgataga tttgcaagt ttttactcg cgttatgtc 
      taccgaaga ataagggat tgctggaac taccccatt 
      tgcagcgtt ccggggaac gaagtggtt ttgaaagac 
      agggtcacg aacgacaac ggatggacc tttgagttt 
      accggctct aaaaccaaa tgcttgaac ttggcctcg 
      tacaattac ctcggtttt gcggaaaat tccggccct 
      tgtgccgag tttgcaatt gacgcgatt tacaaatac 
      ggggtcacc acaggtggt actagacaa cagtacggc 
      acttgtgat ttgcacgtc gaattggaa aaattaact 
      gcggagttt ttgggcgtc gaagacgca attaccttc 
      ggaatgggt tttgcaaca aacgccctc aatattccg 
      acgctgtta tcacccggt tgtctcgtt atcagtgac 
      gagaaaaac cacgcttcg ctcattttg gggattcgt 
      ttaccgggg gccactgtc aaagttttc aaacataac 
      gatgtgaag catctggag caaattttg cgcgagagc 
      atctacaag ggccagccg agccaagag gggtacaag 
      ccgtggaag aaaattttg atcgtggtc gaaggtgtc 
      tacagtatg gagggcaca atcgtccgt ttacctgaa 
      ataatagca cttaaaaag aagtacaaa gcttattta 
      tacctagat gaagcgcat agcatcggt gcgatgggc 
      aagcacggc cgaggcatc gttgattat ttcgggtgc 
      gaccccaaa gatatagat attttgatg gggactttc 
      accaagagt tttggctcc gctgggggc tacatagcc 
      ggaaccaaa gaattgatc gattttttg cggaagaat 
      agttttgct tcgaaatac gcctgggcc atggcgcca 
      cctgtagcc gcacaaatt atcgcagtt ttgaaaata 
      ctgatggga cgggatggt actaatgag ggacagaaa 
      aggatcgaa acgctggca cgcaatacg aggtatttc 
      aggaggagg atcgaacag atggggatt attatacac 
      gggaatgag gattcgccg gttgttccg atccttgtc 
      tacctctac tcgaaaatt gcagctatg gtcaggacg 
      ctgattaag gagaaaatc gcaacggtt ggtgtcgga 
      tatccggcg acgccgttg atggaaggc cggataagg 
      atctgtctg tcagccgca cacaccaaa gaacagctc 
      gactacgcc ttggctgtt atcgaaaaa gttgcagat 
      gagataggg ttgaaatat tcgcgcaag ccgattgat 
      ccaacacct atcgattat gacaaaatc aaggtgtat 
      gtcgactaa
    mRNA sequence
    • >TC008315 RA
      gcgagtcgc aatcaacca acaaaaaat ttgcgtttg 
      taaaatgtg gacgcatgc gtcctcaaa tcctcagta 
      tagaagcga gaatggaag agggtgcag gtgatgctg 
      tgacagagc tactaagtg gggtagatt tcgaaaata 
      ccaaacacc aaaaggtca tttcgaacg gatacggcc 
      cctactcgg gaggctacg caaatggat atacgtgca 
      tcaataaga ggaacgggt acagaggat ccgagcaga 
      atggacatg tgctggcat cgtctgacg aggaatgca 
      ccgccaaaa acatgaagg gaccaataa aaaacggtt 
      atgtaaaaa caactttaa aggaaccgc taaaaaccc 
      gaatattga aagaatcct tcgaaccac cgactctcc 
      tcacagcgg ctttaacgc acattagtt tctatattt 
      taatgtttc tcggttatt tgagccagg ccttgtttc 
      caccaaaga tagtcaaag agaaaaatc gggacggtt 
      atctccctc tgtttgata gatttgcaa gtttttact 
      cgcgttatg tctaccgaa gaataaggg attgctgga 
      actacccca tttgcagcg ttccgggga acgaagtgg 
      ttttgaaag acagggtca cgaacgaca acggatgga 
      cctttgagt ttaccggct ctaaaacca aatgcttga 
      acttggcct cgtacaatt acctcggtt ttgcggaaa 
      attccggcc cttgtgccg agtttgcaa ttgacgcga 
      tttacaaat acggggtca ccacaggtg gtactagac 
      aacagtacg gcacttgtg atttgcacg tcgaattgg 
      aaaaattaa ctgcggagt ttttgggcg tcgaagacg 
      caattacct tcggaatgg gttttgcaa caaacgccc 
      tcaatattc cgacgctgt tatcacccg gttgtctcg 
      ttatcagtg acgagaaaa accacgctt cgctcattt 
      tggggattc gtttaccgg gggccactg tcaaagttt 
      tcaaacata acgatgtga agcatctgg agcaaattt 
      tgcgcgaga gcatctaca agggccagc cgagccaag 
      aggggtaca agccgtgga agaaaattt tgatcgtgg 
      tcgaaggtg tctacagta tggagggca caatcgtcc 
      gtttacctg aaataatag cacttaaaa agaagtaca 
      aagcttatt tatacctag atgaagcgc atagcatcg 
      gtgcgatgg gcaagcacg gccgaggca tcgttgatt 
      atttcgggt gcgacccca aagatatag atattttga 
      tggggactt tcaccaaga gttttggct ccgctgggg 
      gctacatag ccggaacca aagaattga tcgattttt 
      tgcggaaga atagttttg cttcgaaat acgcctggg 
      ccatggcgc cacctgtag ccgcacaaa ttatcgcag 
      ttttgaaaa tactgatgg gacgggatg gtactaatg 
      agggacaga aaaggatcg aaacgctgg cacgcaata 
      cgaggtatt tcaggagga ggatcgaac agatgggga 
      ttattatac acgggaatg aggattcgc cggttgttc 
      cgatccttg tctacctct actcgaaaa ttgcagcta 
      tggtcagga cgctgatta aggagaaaa tcgcaacgg 
      ttggtgtcg gatatccgg cgacgccgt tgatggaag 
      gccggataa ggatctgtc tgtcagccg cacacacca 
      aagaacagc tcgactacg ccttggctg ttatcgaaa 
      aagttgcag atgagatag ggttgaaat attcgcgca 
      agccgattg atccaacac ctatcgatt atgacaaaa 
      tcaaggtgt atgtcgact aaattttaa atcaaattc 
      atagcttaa ttacgtctg cttgttaaa aattttgat 
      acataggca agatatttt tttattgaa tttttgatg 
      gttgaaact tttataaac ctttattac agattatac 
      acatttcgc gtacattgt atattacaa tgtccgtaa 
      catacccag tccataatt ttacatcgg aatttcgtt 
      gtttaactt aatgtaata gtcgtctag tttttgttt 
      gtaaagtgt tttatttat tttttattt atagcgcat 
      gttcattac accgctcta cttgcagat gaatttcag 
      tattgacta aattaaggc acaattaca gtattaacg 
      aaatttctg cttttcata gaatacaag gacttacat 
      atcatagtt gctttatag tctactaca ctagagata 
      atgcttaac aagcaaaaa cgctagaaa ttcgtttaa 
      ttttattta gttatttat attgtgtcg ttttgtcaa 
      ttattttaa ggaaaaaac gttaatcat tcattcccg 
      ttattttta ctgaaatcc tacttattt ttatatttt 
      attgcagtc tctatttta cccgttttg tgttttcta 
      caccttgct gttattgtt aaagattaa aataataaa 
      tctttacat aaaca
    • >TC008315 RB
      cggttgttc agtttctgt tatttgggg aaaattcgg 
      aaagaccgc aagtttttg aatgcaagg ttgtctcac 
      ggtatcacg cgagtcgca atcaaccaa caaaaaatt 
      tgcgtttgt aaaatgtgg acgcatgcg tcctcaaat 
      cctcagtat agaagcgag aatggaaga gggtgcagg 
      tgatgctgt gacagagct actaagtgg ggtagattt 
      cgaaaatac caaacacca aaaggtcat ttcgaacgg 
      atacggccc ctactcggg aggctacgc aaatggata 
      tacgtgcat caataagag gaacgggta cagaggatc 
      cgagcagaa tggacatgt gctggcatc gtctgacga 
      ggaatgcac cgccaaaaa catgaaggg accaataaa 
      aaacggtta tgtaaaaac aactttaaa ggaaccgct 
      aaaaacccg aatattgaa agaatcctt cgaaccacc 
      gactctcct cacagcggc tttaacgca cattagttt 
      ctatatttt aatgtttct cggttattt gagccaggc 
      cttgtttcc accaaagat agtcaaaga gaaaaatcg 
      ggacggtta tctccctct gtttgatag atttgcaag 
      tttttactc gcgttatgt ctaccgaag aataaggga 
      ttgctggaa ctaccccat ttgcagcgt tccggggaa 
      cgaagtggt tttgaaaga cagggtcac gaacgacaa 
      cggatggac ctttgagtt taccggctc taaaaccaa 
      atgcttgaa cttggcctc gtacaatta cctcggttt 
      tgcggaaaa ttccggccc ttgtgccga gtttgcaat 
      tgacgcgat ttacaaata cggggtcac cacaggtgg 
      tactagaca acagtacgg cacttgtga tttgcacgt 
      cgaattgga aaaattaac tgcggagtt tttgggcgt 
      cgaagacgc aattacctt cggaatggg ttttgcaac 
      aaacgccct caatattcc gacgctgtt atcacccgg 
      ttgtctcgt tatcagtga cgagaaaaa ccacgcttc 
      gctcatttt ggggattcg tttaccggg ggccactgt 
      caaagtttt caaacataa cgatgtgaa gcatctgga 
      gcaaatttt gcgcgagag catctacaa gggccagcc 
      gagccaaga ggggtacaa gccgtggaa gaaaatttt 
      gatcgtggt cgaaggtgt ctacagtat ggagggcac 
      aatcgtccg tttacctga aataatagc acttaaaaa 
      gaagtacaa agcttattt atacctaga tgaagcgca 
      tagcatcgg tgcgatggg caagcacgg ccgaggcat 
      cgttgatta tttcgggtg cgaccccaa agatataga 
      tattttgat ggggacttt caccaagag ttttggctc 
      cgctggggg ctacatagc cggaaccaa agaattgat 
      cgatttttt gcggaagaa tagttttgc ttcgaaata 
      cgcctgggc catggcgcc acctgtagc cgcacaaat 
      tatcgcagt tttgaaaat actgatggg acgggatgg 
      tactaatga gggacagaa aaggatcga aacgctggc 
      acgcaatac gaggtattt caggaggag gatcgaaca 
      gatggggat tattataca cgggaatga ggattcgcc 
      ggttgttcc gatccttgt ctacctcta ctcgaaaat 
      tgcagctat ggtcaggac gctgattaa ggagaaaat 
      cgcaacggt tggtgtcgg atatccggc gacgccgtt 
      gatggaagg ccggataag gatctgtct gtcagccgc 
      acacaccaa agaacagct cgactacgc cttggctgt 
      tatcgaaaa agttgcaga tgagatagg gttgaaata 
      ttcgcgcaa gccgattga tccaacacc tatcgatta 
      tgacaaaat caaggtgta tgtcgacta aattttaaa 
      tcaaattca tagcttaat tacgtctgc ttgttaaaa 
      attttgata cataggcaa gatattttt ttattgaat 
      ttttgatgg ttgaaactt ttataaacc tttattaca 
      gattataca catttcgcg tacattgta tattacaat 
      gtccgtaac atacccagt ccataattt tacatcgga 
      atttcgttg tttaactta atgtaatag tcgtctagt 
      ttttgtttg taaagtgtt ttatttatt ttttattta 
      tagcgcatg ttcattaca ccgctctac ttgcagatg 
      aatttcagt attgactaa attaaggca caattacag 
      tattaacga aatttctgc ttttcatag aatacaagg 
      acttacata tcatagttg ctttatagt ctactacac 
      tagagataa tgcttaaca agcaaaaac gctagaaat 
      tcgtttaat tttatttag ttatttata ttgtgtcgt 
      tttgtcaat tattttaag gaaaaaacg ttaatcatt 
      cattcccgt tatttttac tgaaatcct acttatttt 
      tatatttta ttgcagtct ctattttac ccgttttgt 
      gttttctac accttgctg ttattgtta aagattaaa 
      ataataaat ctttacata aaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY