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Gene information
General information
TC007156 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8396002..8404549
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007156
    iBeetle screen iB_07226

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007156 RA
      MAGVVSNDV WKSWEKSGK TEFVKTCMQ LLKDDTKSP 
      VFDKNGRVS SLITNINML IDKGLKGQL KVEAVVSTL 
      QELVSLHPD VPSVILDVL SITDSSCIP DSSNDDSKE 
      RNNLCFIIK ECEKLLSDQ LLKERLEID TLQEVGILK 
      NKTFYSKFI KVKTKLYYK QRKFNLFRE ECEGFAKLQ 
      TELNQEFGE NTSHEDVLD IVQSLIGCF NLDPNRVLD 
      IILESFENK PKDARIFVP LICSYMKDP KIVSEVLAS 
      KFTFLRSSE TNVPRSLYV LTAQLLQHN IISLDDIYL 
      WLTPEDKIM FKECDKAMK DAKEYVRKL QVISINNKE 
      KEETPEEPE NLMEKFNAN QKLGLCEAL LSVGDWNNA 
      KNLINRLPD HYALSFQPI ALALCKLLH YIVEPVYRE 
      FCKLGPKIK GKPIPRHEN PLSPKQATS FLELRDHAI 
      PMLITLGAS LRFDPVLMY KVIRISKAA LNSYGVESS 
      KSPCPSGDT LYYDILTLL DTTILSSLS YMDCNCCIA 
      EEVWTVLKL YPYQNRYCL YSRWKNETY SLYPDLLRK 
      RGDSEKQIK NIMKRVSKE NIKPVGRLI GKLTHCSPG 
      FLFDYILLQ IQVYSNLIH PVVDSLKYL TSLSYDVLG 
      FCLVECLAN ADKKRVKHD STSISSWLQ SLSSFCGAV 
      YKKYIIELT GLLQYVANQ LKAQKSLDL LILKEIVQK 
      MAGVEAAED LTMEQVNAM AGGELLKAE AGYLSQVRN 
      TKKSSLRLK EAMSEHNLA VALCLLMAQ QNYCVVYRE 
      TQKNHLKLV GKLADQCQD TLVQFGTFL GSSLSVDEY 
      VNKLPSIQS MLLDYHIPN EVAFFLARP MFNHAINIK 
      YDQLRKSDP NYKKLSTNV KNQKYFEAV NEVMEPVHK 
      SIIPLHPPK IWEDISPQF LSTFWSLTM YDLYVPDET 
      YQQVVNKTR QQSLATIES SNNKGKKEQ ERYLTLVEK 
      LQDEKKKQH EHVEKVMYR LKQEKDSWF LSRSAKSAK 
      NETITRFLQ LCLFPRCTF TSIDAVYCA KFVHVIHML 
      KTPNFSTLL CYDRLFCDV TYSITSCTE NEAMRYGRF 
      LYAMLETVM RWHKSKELF EKECANYPG FVTKYRLNN 
      QHADNLDNV GYENYRHVC HKWHYKVAK AMVTCLDSK 
      DYVQIRNSL IILIKIIPF FPVLQKVGQ FIEKRIEKV 
      RDEEKNNRQ DLFTLSSSY LGLLKQRIM SGHMVKESD 
      FHILVEKEK VVRSHEAKS EAGSSKHNG EVKEEMKDK 
      SEKKIKAED LKVVSRTSQ GSQSSGDYN AWERPEPRE 
      KHRREEKRD DFEKERERE KRRERKEEK LAKQEERQY 
      REERYSEIS PKDEMRYSS DRQVERYYD ERGSHYYRE 
      VRDEREIGL SGSNNGSSS MGRHSQEPE TDREVKRRK 
      MDGSSLKSS KHEERKLQQ LDYMLEKTD KKERSGKAK 
      EKREKLSDE EKELRKERK LGRKRDRTE EALQVEMKR 
      RREEEKAAK LSHQNGEIM EPVHQREKH HRSRDLSPY 
      PRERSHDDR LEMREKHRR SAENKRR
    CDS sequence
    • >TC007156 RA
      atggcaggt gttgttagt aacgatgtt tggaaaagt 
      tgggaaaaa tcgggaaaa acagagttt gttaaaaca 
      tgcatgcag ttgttgaaa gatgacacc aagtccccg 
      gtttttgac aaaaatggg agagtctca agtctcata 
      accaacata aatatgttg attgacaaa ggactcaaa 
      gggcagttg aaagtggag gctgttgtt tcaacgctc 
      caggaactt gtttcttta catcctgat gttccttcg 
      gtaatttta gacgtttta agtatcaca gactcttct 
      tgcatacct gatagcagt aacgacgat tcaaaagaa 
      aggaacaat ttgtgtttt atcataaaa gaatgtgaa 
      aagttgctt tcagaccaa ttgttgaag gaacggtta 
      gaaatagac acgctacaa gaagttggg atcttgaaa 
      aacaaaacg ttttattca aagtttatt aaagtgaag 
      acaaaacta tattacaaa cagcgcaaa tttaactta 
      tttcgcgag gaatgtgag ggttttgca aaattacaa 
      acggaattg aaccaagag tttggcgaa aacaccagt 
      catgaggac gtcctagac attgttcaa tcattaatt 
      ggttgcttc aacctcgac ccaaaccga gtccttgat 
      ataattctg gagagtttt gagaataaa ccaaaagac 
      gcgcgaatt ttcgttcct ctaatatgc tcgtatatg 
      aaagacccg aaaatcgtg agtgaagtt ctagcatcg 
      aaattcact tttttgcga agcagcgaa acaaatgtt 
      cctcgatct ctatacgta ttaacggcg cagttgtta 
      caacataac attatatca ctggacgac atttatctt 
      tggttaaca cctgaggat aaaatcatg tttaaagag 
      tgcgataaa gcaatgaaa gatgcaaaa gaatacgtg 
      cgtaagcta caagtgatc tcgataaat aataaggag 
      aaggaagaa acgcccgaa gagccggaa aatctcatg 
      gaaaaattt aacgcgaac caaaagttg gggctttgc 
      gaggcgttg ctttcagta ggtgattgg aacaatgcg 
      aaaaatttg ataaaccgt ctgcctgat cactacgca 
      ctgtcattc caaccgatc gcgttagca ctgtgcaaa 
      ttgttgcat tacatcgtt gaacctgtt tatagagaa 
      ttttgtaag ttgggacca aaaatcaag ggaaaacct 
      atcccgcgc catgaaaat cccctcagc ccgaaacaa 
      gcaacctca tttttggaa cttcgagat catgccatt 
      cctatgttg attacttta ggagcttcg ctgcgtttt 
      gatcccgtt ttgatgtac aaagtgata agaatttcc 
      aaagcggcg ttaaattcc tatggagta gagtcgtca 
      aaatcgcct tgtccgtct ggtgatacg ttatattac 
      gacattttg actttgttg gacacgaca atactgtcg 
      tcactctcg tacatggat tgcaattgt tgcatcgca 
      gaggaagtg tggacggtc ctcaaactc tacccatat 
      cagaatagg tactgtctt tacagccgg tggaaaaac 
      gagacgtat tctttatac cccgacttg ttacgaaaa 
      cgcggagat tccgaaaaa caaatcaaa aatatcatg 
      aaacgagtc agtaaggaa aacatcaaa cctgtaggc 
      cgcctcatt ggcaaactc acccactgt tcacccgga 
      tttctgttc gattatatt ttactccaa attcaagtt 
      tatagcaat ttgattcac ccagtcgtc gactctttg 
      aaatatctg acgagttta tcgtacgat gttttgggc 
      ttttgcctc gttgaatgt ctagcgaac gccgacaag 
      aaacgggta aaacatgat agcacttcg atctcttcg 
      tggctacaa agtctctcg agtttttgt ggcgctgtt 
      tacaaaaaa tacatcata gaattgacc gggcttttg 
      cagtatgtg gccaaccaa cttaaagcg caaaaaagt 
      ttggatttg ctgattttg aaagaaatc gtccagaaa 
      atggcaggt gttgaagcc gccgaagac ttgaccatg 
      gagcaagtg aacgcaatg gcagggggc gaacttctg 
      aaagccgag gccggttat ttgagtcaa gttcgcaac 
      actaaaaaa tcatcactg cggcttaag gaagcaatg 
      tctgagcat aatctagct gtggctttg tgtttactg 
      atggcccag caaaattat tgtgttgtc tatcgggaa 
      acgcagaag aatcatttg aaattggtc gggaaattg 
      gccgatcaa tgtcaggac actttggtc cagtttggg 
      acgtttctc ggctcttct ctttcagtt gatgagtat 
      gtgaataaa ttgccaagt atccagagt atgctcctc 
      gattatcat attccaaac gaagttgcc ttcttcctg 
      gccaggccc atgttcaac catgccata aatataaag 
      tacgatcag ttgcgtaaa tctgaccct aactacaag 
      aaactctca acgaacgta aaaaaccag aaatatttc 
      gaagcggtg aacgaagtg atggaaccg gtccacaaa 
      tctataatt ccgctccac cccccgaaa atctgggag 
      gacatttcg ccccaattt ttatcaacc ttttggtct 
      ttaacaatg tatgatctt tacgtacct gacgaaacg 
      taccaacaa gtcgtgaat aaaacgcgc caacaatcg 
      ttggcaacg atagaatcg agtaacaac aaaggtaaa 
      aaagagcaa gaacgttac ttgacccta gtcgagaaa 
      ctgcaagac gagaagaag aaacaacac gaacacgtg 
      gagaaagta atgtatagg ttgaagcaa gagaaggac 
      agttggttc ctgtcaaga tcggcaaaa tcggccaaa 
      aacgaaaca ataacccgg tttttgcag ttgtgtttg 
      ttccctaga tgcactttc acgtcgatc gatgccgtg 
      tattgcgct aagttcgtc cacgtgatt cacatgctg 
      aaaacgccg aatttttcg acgcttttg tgctacgac 
      aggctcttt tgcgacgtc acatattcc attacttcg 
      tgtaccgaa aatgaggcg atgcgttat gggcgattt 
      ctgtacgca atgctcgag actgtaatg cggtggcac 
      aaatcgaaa gagttgttt gagaaagaa tgtgccaat 
      tatccaggt tttgttact aaatatcgt ctgaataac 
      caacatgcc gacaatttg gataatgtc ggttatgag 
      aattacagg catgtttgt cataaatgg cactataaa 
      gtagcaaag gctatggtc acgtgcttg gactcgaaa 
      gattacgta caaatcagg aactcgtta attatccta 
      attaagatc attccattt ttccccgtg ttgcaaaag 
      gtggggcaa tttattgaa aaacggatc gaaaaagtg 
      cgagatgag gagaagaac aatcgacag gatttgttc 
      acgttgtct agtagttac ttggggttg ttgaaacag 
      aggattatg agcgggcat atggtgaaa gagagcgat 
      ttccatatt ttggtggag aaagagaag gttgtgaga 
      agtcatgaa gctaaaagt gaggcaggt tctagcaaa 
      cataacggc gaagttaaa gaagagatg aaggacaaa 
      agcgagaaa aaaatcaaa gcggaggat cttaaagtc 
      gtctcacga acatcgcaa ggttcccaa agctctggc 
      gattataac gcgtgggaa cgccccgaa ccaagggaa 
      aaacacaga agggaggag aaacgtgac gattttgag 
      aaagaacgc gaaagagag aaacgtaga gagcgtaaa 
      gaggagaaa ttggcgaaa caggaagaa cgacagtat 
      cgagaggag cgctattct gaaatcagt ccgaaagat 
      gaaatgcgg tattcaagc gacagacaa gtggaacga 
      tactatgac gaaagaggc agtcactac tatcgagaa 
      gtgcgagat gaacgcgaa atcgggttg agtggtagc 
      aataatggg tcaagtagt atgggaagg catagtcaa 
      gagcccgag accgataga gaagttaaa agaagaaaa 
      atggatggt tcttctttg aagagttcg aaacacgaa 
      gagaggaaa ctacaacaa ctcgattac atgttagag 
      aaaacggac aaaaaagaa aggtcgggc aaagcgaaa 
      gagaagagg gaaaagttg agcgacgag gaaaaagag 
      ttgaggaaa gaacggaag ttgggaagg aaaagggat 
      cggaccgaa gaagcgctc caagttgaa atgaagagg 
      cgaagagaa gaagaaaaa gcggcgaaa ctttcacac 
      caaaatgga gaaatcatg gaacccgtc caccaaaga 
      gaaaaacat catcgctcg agagacttg tcaccatat 
      ccacgagag aggtcgcac gatgaccga ctcgaaatg 
      agggaaaaa cacagaaga agtgccgaa aataaaagg 
      agatga
    mRNA sequence
    • >TC007156 RA
      catgttggg aaaaatgac gcttggttg ctaaaaatg 
      gcaggtgtt gttagtaac gatgtttgg aaaagttgg 
      gaaaaatcg ggaaaaaca gagtttgtt aaaacatgc 
      atgcagttg ttgaaagat gacaccaag tccccggtt 
      tttgacaaa aatgggaga gtctcaagt ctcataacc 
      aacataaat atgttgatt gacaaagga ctcaaaggg 
      cagttgaaa gtggaggct gttgtttca acgctccag 
      gaacttgtt tctttacat cctgatgtt ccttcggta 
      attttagac gttttaagt atcacagac tcttcttgc 
      atacctgat agcagtaac gacgattca aaagaaagg 
      aacaatttg tgttttatc ataaaagaa tgtgaaaag 
      ttgctttca gaccaattg ttgaaggaa cggttagaa 
      atagacacg ctacaagaa gttgggatc ttgaaaaac 
      aaaacgttt tattcaaag tttattaaa gtgaagaca 
      aaactatat tacaaacag cgcaaattt aacttattt 
      cgcgaggaa tgtgagggt tttgcaaaa ttacaaacg 
      gaattgaac caagagttt ggcgaaaac accagtcat 
      gaggacgtc ctagacatt gttcaatca ttaattggt 
      tgcttcaac ctcgaccca aaccgagtc cttgatata 
      attctggag agttttgag aataaacca aaagacgcg 
      cgaattttc gttcctcta atatgctcg tatatgaaa 
      gacccgaaa atcgtgagt gaagttcta gcatcgaaa 
      ttcactttt ttgcgaagc agcgaaaca aatgttcct 
      cgatctcta tacgtatta acggcgcag ttgttacaa 
      cataacatt atatcactg gacgacatt tatctttgg 
      ttaacacct gaggataaa atcatgttt aaagagtgc 
      gataaagca atgaaagat gcaaaagaa tacgtgcgt 
      aagctacaa gtgatctcg ataaataat aaggagaag 
      gaagaaacg cccgaagag ccggaaaat ctcatggaa 
      aaatttaac gcgaaccaa aagttgggg ctttgcgag 
      gcgttgctt tcagtaggt gattggaac aatgcgaaa 
      aatttgata aaccgtctg cctgatcac tacgcactg 
      tcattccaa ccgatcgcg ttagcactg tgcaaattg 
      ttgcattac atcgttgaa cctgtttat agagaattt 
      tgtaagttg ggaccaaaa atcaaggga aaacctatc 
      ccgcgccat gaaaatccc ctcagcccg aaacaagca 
      acctcattt ttggaactt cgagatcat gccattcct 
      atgttgatt actttagga gcttcgctg cgttttgat 
      cccgttttg atgtacaaa gtgataaga atttccaaa 
      gcggcgtta aattcctat ggagtagag tcgtcaaaa 
      tcgccttgt ccgtctggt gatacgtta tattacgac 
      attttgact ttgttggac acgacaata ctgtcgtca 
      ctctcgtac atggattgc aattgttgc atcgcagag 
      gaagtgtgg acggtcctc aaactctac ccatatcag 
      aataggtac tgtctttac agccggtgg aaaaacgag 
      acgtattct ttatacccc gacttgtta cgaaaacgc 
      ggagattcc gaaaaacaa atcaaaaat atcatgaaa 
      cgagtcagt aaggaaaac atcaaacct gtaggccgc 
      ctcattggc aaactcacc cactgttca cccggattt 
      ctgttcgat tatatttta ctccaaatt caagtttat 
      agcaatttg attcaccca gtcgtcgac tctttgaaa 
      tatctgacg agtttatcg tacgatgtt ttgggcttt 
      tgcctcgtt gaatgtcta gcgaacgcc gacaagaaa 
      cgggtaaaa catgatagc acttcgatc tcttcgtgg 
      ctacaaagt ctctcgagt ttttgtggc gctgtttac 
      aaaaaatac atcatagaa ttgaccggg cttttgcag 
      tatgtggcc aaccaactt aaagcgcaa aaaagtttg 
      gatttgctg attttgaaa gaaatcgtc cagaaaatg 
      gcaggtgtt gaagccgcc gaagacttg accatggag 
      caagtgaac gcaatggca gggggcgaa cttctgaaa 
      gccgaggcc ggttatttg agtcaagtt cgcaacact 
      aaaaaatca tcactgcgg cttaaggaa gcaatgtct 
      gagcataat ctagctgtg gctttgtgt ttactgatg 
      gcccagcaa aattattgt gttgtctat cgggaaacg 
      cagaagaat catttgaaa ttggtcggg aaattggcc 
      gatcaatgt caggacact ttggtccag tttgggacg 
      tttctcggc tcttctctt tcagttgat gagtatgtg 
      aataaattg ccaagtatc cagagtatg ctcctcgat 
      tatcatatt ccaaacgaa gttgccttc ttcctggcc 
      aggcccatg ttcaaccat gccataaat ataaagtac 
      gatcagttg cgtaaatct gaccctaac tacaagaaa 
      ctctcaacg aacgtaaaa aaccagaaa tatttcgaa 
      gcggtgaac gaagtgatg gaaccggtc cacaaatct 
      ataattccg ctccacccc ccgaaaatc tgggaggac 
      atttcgccc caattttta tcaaccttt tggtcttta 
      acaatgtat gatctttac gtacctgac gaaacgtac 
      caacaagtc gtgaataaa acgcgccaa caatcgttg 
      gcaacgata gaatcgagt aacaacaaa ggtaaaaaa 
      gagcaagaa cgttacttg accctagtc gagaaactg 
      caagacgag aagaagaaa caacacgaa cacgtggag 
      aaagtaatg tataggttg aagcaagag aaggacagt 
      tggttcctg tcaagatcg gcaaaatcg gccaaaaac 
      gaaacaata acccggttt ttgcagttg tgtttgttc 
      cctagatgc actttcacg tcgatcgat gccgtgtat 
      tgcgctaag ttcgtccac gtgattcac atgctgaaa 
      acgccgaat ttttcgacg cttttgtgc tacgacagg 
      ctcttttgc gacgtcaca tattccatt acttcgtgt 
      accgaaaat gaggcgatg cgttatggg cgatttctg 
      tacgcaatg ctcgagact gtaatgcgg tggcacaaa 
      tcgaaagag ttgtttgag aaagaatgt gccaattat 
      ccaggtttt gttactaaa tatcgtctg aataaccaa 
      catgccgac aatttggat aatgtcggt tatgagaat 
      tacaggcat gtttgtcat aaatggcac tataaagta 
      gcaaaggct atggtcacg tgcttggac tcgaaagat 
      tacgtacaa atcaggaac tcgttaatt atcctaatt 
      aagatcatt ccatttttc cccgtgttg caaaaggtg 
      gggcaattt attgaaaaa cggatcgaa aaagtgcga 
      gatgaggag aagaacaat cgacaggat ttgttcacg 
      ttgtctagt agttacttg gggttgttg aaacagagg 
      attatgagc gggcatatg gtgaaagag agcgatttc 
      catattttg gtggagaaa gagaaggtt gtgagaagt 
      catgaagct aaaagtgag gcaggttct agcaaacat 
      aacggcgaa gttaaagaa gagatgaag gacaaaagc 
      gagaaaaaa atcaaagcg gaggatctt aaagtcgtc 
      tcacgaaca tcgcaaggt tcccaaagc tctggcgat 
      tataacgcg tgggaacgc cccgaacca agggaaaaa 
      cacagaagg gaggagaaa cgtgacgat tttgagaaa 
      gaacgcgaa agagagaaa cgtagagag cgtaaagag 
      gagaaattg gcgaaacag gaagaacga cagtatcga 
      gaggagcgc tattctgaa atcagtccg aaagatgaa 
      atgcggtat tcaagcgac agacaagtg gaacgatac 
      tatgacgaa agaggcagt cactactat cgagaagtg 
      cgagatgaa cgcgaaatc gggttgagt ggtagcaat 
      aatgggtca agtagtatg ggaaggcat agtcaagag 
      cccgagacc gatagagaa gttaaaaga agaaaaatg 
      gatggttct tctttgaag agttcgaaa cacgaagag 
      aggaaacta caacaactc gattacatg ttagagaaa 
      acggacaaa aaagaaagg tcgggcaaa gcgaaagag 
      aagagggaa aagttgagc gacgaggaa aaagagttg 
      aggaaagaa cggaagttg ggaaggaaa agggatcgg 
      accgaagaa gcgctccaa gttgaaatg aagaggcga 
      agagaagaa gaaaaagcg gcgaaactt tcacaccaa 
      aatggagaa atcatggaa cccgtccac caaagagaa 
      aaacatcat cgctcgaga gacttgtca ccatatcca 
      cgagagagg tcgcacgat gaccgactc gaaatgagg 
      gaaaaacac agaagaagt gccgaaaat aaaaggaga 
      tgataattt ttgcggatg gcgatttaa ttttatttt 
      tagtaactt taagtgtat tacacatgg attgtacgt 
      tactgatgt gatctgtag aatatgaga ataaacatg 
      gtaatgtgg t
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