Status
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Gene information
General information
TC032879
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007180
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8014182..8018932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032879
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07224

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature lateral size shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032879 RA
      MLLLLLLAA SRAEHVRDL EVSEGAPVG TRIGFIGDG 
      ASDSGPPYL IVPVGSAVD SDLNIDQTT GEIRTKVPL 
      DRETRSSYS LVAIPIRGD NVKVVVKVL DENDNAPTF 
      PTSVMSIEF PENTPRDVK RTLHPARDL DLDIFNTQR 
      YNIVSGNIN NTFRLSSHR ERDGVLYLD LQINGFLDR 
      ETTPAYSLV IEALDGGTP PLRGEMRVN ITIQDVNDN 
      QPIFNQSRY IATVPENAT VGTTVLQVF ATDKDSGEN 
      GQIEYSINR RQSDRDNMF RIDPTTGLI VVNKPLDFE 
      TKELHELVI VAKDHGLQP LETTAFVSI RVTDVNDNQ 
      PTINVIFLS DDATPKISE SAQPGEFVA RISVNDPDS 
      KTEYSNINV TLSGGDGHF GLTTRDNII YLVIVSLPL 
      DRELQPNYT LDVVATDRG NPPLHASRT INLMVTDIN 
      DNAPEFDQE VYQANVMEV SDPGTSVIQ VLATDKDEG 
      NNSAISYSL LDTVETHSS WFQIDPRSG LVTTRAHVD 
      CETDPVPRL TVVATDNGF PPLSSSATV LVTIHDVND 
      NEPIFDQSF YNVSVAENE AEGRCILKV RKNGGRGGG 
      NPPWNV
    CDS sequence
    • >TC032879 RA
      atgctgctg ctcctgctc ctggccgcg tccagggcg 
      gagcacgtg cgcgacctc gaggtctcc gaaggggcg 
      cccgtcggc accaggatt ggattcata ggtgatggg 
      gcttccgac agcgggccg ccctatctc atcgttccg 
      gtgggaagt gccgtcgat tcggacctc aacatcgac 
      caaacgacg ggggaaata cggactaaa gtgcctttg 
      gatcgcgaa acgcggtcg agttattcc cttgtggcc 
      atccctatc agaggtgac aatgtgaaa gtggttgtt 
      aaagtgctc gatgaaaac gacaacgct cccacgttt 
      ccgacgtcg gttatgagc atcgagttc cccgaaaac 
      acgccgaga gacgtcaaa cggactcta cacccggcg 
      agagaccta gatcttgac attttcaac acacaaaga 
      tacaacatt gtttcgggg aatatcaac aacacgttc 
      aggttatcg tcacacagg gaacgcgac ggagtgctt 
      tatttagac ctgcagatt aacgggttc ttagataga 
      gaaacaacg ccagcttat agcctcgtt atagaggca 
      ttagatggt ggaactcct cctctcaga ggcgagatg 
      agggtcaat ataacgatc caggatgtt aatgataat 
      caaccgatt tttaatcaa agtagatac attgcgacc 
      gttcccgaa aacgccacc gttggcaca actgtcctc 
      caggtcttc gccaccgat aaagattcc ggagagaac 
      ggtcaaatc gaatattcg atcaatcgg agacaaagt 
      gatcgagat aacatgttt agaatcgat ccgacaacc 
      ggtctcatc gtagtcaac aaaccctta gactttgaa 
      actaaggaa ctccacgaa ctcgtcatc gtagccaaa 
      gaccatggt ttacaaccg ctagaaacc accgctttc 
      gtctcaatc agagtaaca gacgtcaat gataatcag 
      cccacaatc aacgtcatt ttcttgagc gatgatgct 
      actccgaaa atcagcgaa tcggcacaa cctggtgaa 
      ttcgtagcg aggatttct gtcaacgat cccgattcc 
      aaaacggaa tactccaac ataaatgtc acactgagt 
      ggaggtgac ggccatttc gggttgacc acaagagat 
      aacatcatt tatttggta atagtgtct ttgcctctg 
      gacagggaa ttgcagccg aattacact ttggatgtg 
      gtcgccacc gatcgggga aaccccccg ctccacgcc 
      tccaggacc ataaatctg atggtgacg gacattaac 
      gacaacgcg cccgaattc gaccaagaa gtgtaccaa 
      gcaaacgtg atggaagtg tccgatcct ggaacttcc 
      gtgatccag gtcttggcc accgacaag gacgagggc 
      aacaacagt gcgatcagc tactcgctg ctggacacc 
      gtcgagacg cattcgtcc tggttccag atcgaccct 
      cgcagtggt ttggtgacg acccgcgct cacgtcgat 
      tgtgaaacg gatccggtg ccgcgtctg accgtagtt 
      gctacagat aacggtttt ccgccgttg tcgtcttca 
      gcgacagtg ctagtgacc atccacgac gtcaacgac 
      aacgagccc atcttcgac cagagtttc tacaatgtt 
      tcggtggcc gaaaacgag gccgaagga cgctgtatt 
      ctaaaggta aggaaaaac ggcggccga ggtggtggg 
      aatcctcca tggaacgtc tag
    mRNA sequence
    • >TC032879 RA
      gtgctctgt accttcgcg cgattaatc atttcgatc 
      taatataag ttgtaattt gtattgcca gccaaagat 
      ggaatattg caaggctcg agctcagtc catctcaag 
      accgaagcc gaagagtcg gtccgcgtc cttttcgct 
      gtttcgcca gtcaccgcg tcctagtgc gacggcgac 
      gccgtctcc ggagtgcca gtgatctcg agtgagtgc 
      gcgccccga caccgaagc gcccggagt cgacgactc 
      ctcctcctc tctttaaaa cctccggag agaacacgt 
      cgtccgggt cgcaacgcg taactagcc gtcgttcgt 
      gcacccgtt taatccccg gaacggcac cttcaaaag 
      aatccggta aattgagga accattaca aacatattt 
      agagagcga gcgcgcacc aacggcccc gtcgcatgt 
      tttcaggcc cgctagtag cgcttttgt tctggttat 
      tagcagctc catatcaag ggatgcacc agccgtcca 
      ggccgctcc accaggatg cgtgaacgt ccgaaatga 
      cgctgtcga cgatgctgc tgctcctgc tcctggccg 
      cgtccaggg cggagcacg tgcgcgacc tcgaggtct 
      ccgaagggg cgcccgtcg gcaccagga ttggattca 
      taggtgatg gggcttccg acagcgggc cgccctatc 
      tcatcgttc cggtgggaa gtgccgtcg attcggacc 
      tcaacatcg accaaacga cgggggaaa tacggacta 
      aagtgcctt tggatcgcg aaacgcggt cgagttatt 
      cccttgtgg ccatcccta tcagaggtg acaatgtga 
      aagtggttg ttaaagtgc tcgatgaaa acgacaacg 
      ctcccacgt ttccgacgt cggttatga gcatcgagt 
      tccccgaaa acacgccga gagacgtca aacggactc 
      tacacccgg cgagagacc tagatcttg acattttca 
      acacacaaa gatacaaca ttgtttcgg ggaatatca 
      acaacacgt tcaggttat cgtcacaca gggaacgcg 
      acggagtgc tttatttag acctgcaga ttaacgggt 
      tcttagata gagaaacaa cgccagctt atagcctcg 
      ttatagagg cattagatg gtggaactc ctcctctca 
      gaggcgaga tgagggtca atataacga tccaggatg 
      ttaatgata atcaaccga tttttaatc aaagtagat 
      acattgcga ccgttcccg aaaacgcca ccgttggca 
      caactgtcc tccaggtct tcgccaccg ataaagatt 
      ccggagaga acggtcaaa tcgaatatt cgatcaatc 
      ggagacaaa gtgatcgag ataacatgt ttagaatcg 
      atccgacaa ccggtctca tcgtagtca acaaaccct 
      tagactttg aaactaagg aactccacg aactcgtca 
      tcgtagcca aagaccatg gtttacaac cgctagaaa 
      ccaccgctt tcgtctcaa tcagagtaa cagacgtca 
      atgataatc agcccacaa tcaacgtca ttttcttga 
      gcgatgatg ctactccga aaatcagcg aatcggcac 
      aacctggtg aattcgtag cgaggattt ctgtcaacg 
      atcccgatt ccaaaacgg aatactcca acataaatg 
      tcacactga gtggaggtg acggccatt tcgggttga 
      ccacaagag ataacatca tttatttgg taatagtgt 
      ctttgcctc tggacaggg aattgcagc cgaattaca 
      ctttggatg tggtcgcca ccgatcggg gaaaccccc 
      cgctccacg cctccagga ccataaatc tgatggtga 
      cggacatta acgacaacg cgcccgaat tcgaccaag 
      aagtgtacc aagcaaacg tgatggaag tgtccgatc 
      ctggaactt ccgtgatcc aggtcttgg ccaccgaca 
      aggacgagg gcaacaaca gtgcgatca gctactcgc 
      tgctggaca ccgtcgaga cgcattcgt cctggttcc 
      agatcgacc ctcgcagtg gtttggtga cgacccgcg 
      ctcacgtcg attgtgaaa cggatccgg tgccgcgtc 
      tgaccgtag ttgctacag ataacggtt ttccgccgt 
      tgtcgtctt cagcgacag tgctagtga ccatccacg 
      acgtcaacg acaacgagc ccatcttcg accagagtt 
      tctacaatg tttcggtgg ccgaaaacg aggccgaag 
      gacgctgta ttctaaagg taaggaaaa acggcggcc 
      gaggtggtg ggaatcctc catggaacg tctagtaaa 
      aacgcgtcg atcaaaaac gaccaaaca aaagtaaaa 
      gtttaaccg cgaatgaca ctgttaatt ggacaggag 
      gtatgacac ggtgggtta ccgcgaacg gacgagata 
      tgcgacata tttcaagtg tttgcattg ctgacaaca 
      cgcagccga tgacgacgt taatggctt cacgaccag 
      ggttttctc ggcgatcga tatttcgac ggcgacgac 
      ggccaccca tcgagggtg ctcgcgacc ggctcgact 
      tcgaggacc agcgtttcc taatataaa atttattaa 
      gtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY