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Gene information
General information
TC032879
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007180 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8014182..8018932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032879
    iBeetle screen iB_07224

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature lateral size shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032879 RA
      MLLLLLLAA SRAEHVRDL EVSEGAPVG TRIGFIGDG 
ASDSGPPYL IVPVGSAVD SDLNIDQTT GEIRTKVPL 
DRETRSSYS LVAIPIRGD NVKVVVKVL DENDNAPTF 
PTSVMSIEF PENTPRDVK RTLHPARDL DLDIFNTQR 
YNIVSGNIN NTFRLSSHR ERDGVLYLD LQINGFLDR 
ETTPAYSLV IEALDGGTP PLRGEMRVN ITIQDVNDN 
QPIFNQSRY IATVPENAT VGTTVLQVF ATDKDSGEN 
GQIEYSINR RQSDRDNMF RIDPTTGLI VVNKPLDFE 
TKELHELVI VAKDHGLQP LETTAFVSI RVTDVNDNQ 
PTINVIFLS DDATPKISE SAQPGEFVA RISVNDPDS 
KTEYSNINV TLSGGDGHF GLTTRDNII YLVIVSLPL 
DRELQPNYT LDVVATDRG NPPLHASRT INLMVTDIN 
DNAPEFDQE VYQANVMEV SDPGTSVIQ VLATDKDEG 
NNSAISYSL LDTVETHSS WFQIDPRSG LVTTRAHVD 
CETDPVPRL TVVATDNGF PPLSSSATV LVTIHDVND 
NEPIFDQSF YNVSVAENE AEGRCILKV RKNGGRGGG 
NPPWNV
    CDS sequence
    • >TC032879 RA
      atgctgctg ctcctgctc ctggccgcg tccagggcg 
gagcacgtg cgcgacctc gaggtctcc gaaggggcg 
cccgtcggc accaggatt ggattcata ggtgatggg 
gcttccgac agcgggccg ccctatctc atcgttccg 
gtgggaagt gccgtcgat tcggacctc aacatcgac 
caaacgacg ggggaaata cggactaaa gtgcctttg 
gatcgcgaa acgcggtcg agttattcc cttgtggcc 
atccctatc agaggtgac aatgtgaaa gtggttgtt 
aaagtgctc gatgaaaac gacaacgct cccacgttt 
ccgacgtcg gttatgagc atcgagttc cccgaaaac 
acgccgaga gacgtcaaa cggactcta cacccggcg 
agagaccta gatcttgac attttcaac acacaaaga 
tacaacatt gtttcgggg aatatcaac aacacgttc 
aggttatcg tcacacagg gaacgcgac ggagtgctt 
tatttagac ctgcagatt aacgggttc ttagataga 
gaaacaacg ccagcttat agcctcgtt atagaggca 
ttagatggt ggaactcct cctctcaga ggcgagatg 
agggtcaat ataacgatc caggatgtt aatgataat 
caaccgatt tttaatcaa agtagatac attgcgacc 
gttcccgaa aacgccacc gttggcaca actgtcctc 
caggtcttc gccaccgat aaagattcc ggagagaac 
ggtcaaatc gaatattcg atcaatcgg agacaaagt 
gatcgagat aacatgttt agaatcgat ccgacaacc 
ggtctcatc gtagtcaac aaaccctta gactttgaa 
actaaggaa ctccacgaa ctcgtcatc gtagccaaa 
gaccatggt ttacaaccg ctagaaacc accgctttc 
gtctcaatc agagtaaca gacgtcaat gataatcag 
cccacaatc aacgtcatt ttcttgagc gatgatgct 
actccgaaa atcagcgaa tcggcacaa cctggtgaa 
ttcgtagcg aggatttct gtcaacgat cccgattcc 
aaaacggaa tactccaac ataaatgtc acactgagt 
ggaggtgac ggccatttc gggttgacc acaagagat 
aacatcatt tatttggta atagtgtct ttgcctctg 
gacagggaa ttgcagccg aattacact ttggatgtg 
gtcgccacc gatcgggga aaccccccg ctccacgcc 
tccaggacc ataaatctg atggtgacg gacattaac 
gacaacgcg cccgaattc gaccaagaa gtgtaccaa 
gcaaacgtg atggaagtg tccgatcct ggaacttcc 
gtgatccag gtcttggcc accgacaag gacgagggc 
aacaacagt gcgatcagc tactcgctg ctggacacc 
gtcgagacg cattcgtcc tggttccag atcgaccct 
cgcagtggt ttggtgacg acccgcgct cacgtcgat 
tgtgaaacg gatccggtg ccgcgtctg accgtagtt 
gctacagat aacggtttt ccgccgttg tcgtcttca 
gcgacagtg ctagtgacc atccacgac gtcaacgac 
aacgagccc atcttcgac cagagtttc tacaatgtt 
tcggtggcc gaaaacgag gccgaagga cgctgtatt 
ctaaaggta aggaaaaac ggcggccga ggtggtggg 
aatcctcca tggaacgtc tag
    mRNA sequence
    • >TC032879 RA
      gtgctctgt accttcgcg cgattaatc atttcgatc 
taatataag ttgtaattt gtattgcca gccaaagat 
ggaatattg caaggctcg agctcagtc catctcaag 
accgaagcc gaagagtcg gtccgcgtc cttttcgct 
gtttcgcca gtcaccgcg tcctagtgc gacggcgac 
gccgtctcc ggagtgcca gtgatctcg agtgagtgc 
gcgccccga caccgaagc gcccggagt cgacgactc 
ctcctcctc tctttaaaa cctccggag agaacacgt 
cgtccgggt cgcaacgcg taactagcc gtcgttcgt 
gcacccgtt taatccccg gaacggcac cttcaaaag 
aatccggta aattgagga accattaca aacatattt 
agagagcga gcgcgcacc aacggcccc gtcgcatgt 
tttcaggcc cgctagtag cgcttttgt tctggttat 
tagcagctc catatcaag ggatgcacc agccgtcca 
ggccgctcc accaggatg cgtgaacgt ccgaaatga 
cgctgtcga cgatgctgc tgctcctgc tcctggccg 
cgtccaggg cggagcacg tgcgcgacc tcgaggtct 
ccgaagggg cgcccgtcg gcaccagga ttggattca 
taggtgatg gggcttccg acagcgggc cgccctatc 
tcatcgttc cggtgggaa gtgccgtcg attcggacc 
tcaacatcg accaaacga cgggggaaa tacggacta 
aagtgcctt tggatcgcg aaacgcggt cgagttatt 
cccttgtgg ccatcccta tcagaggtg acaatgtga 
aagtggttg ttaaagtgc tcgatgaaa acgacaacg 
ctcccacgt ttccgacgt cggttatga gcatcgagt 
tccccgaaa acacgccga gagacgtca aacggactc 
tacacccgg cgagagacc tagatcttg acattttca 
acacacaaa gatacaaca ttgtttcgg ggaatatca 
acaacacgt tcaggttat cgtcacaca gggaacgcg 
acggagtgc tttatttag acctgcaga ttaacgggt 
tcttagata gagaaacaa cgccagctt atagcctcg 
ttatagagg cattagatg gtggaactc ctcctctca 
gaggcgaga tgagggtca atataacga tccaggatg 
ttaatgata atcaaccga tttttaatc aaagtagat 
acattgcga ccgttcccg aaaacgcca ccgttggca 
caactgtcc tccaggtct tcgccaccg ataaagatt 
ccggagaga acggtcaaa tcgaatatt cgatcaatc 
ggagacaaa gtgatcgag ataacatgt ttagaatcg 
atccgacaa ccggtctca tcgtagtca acaaaccct 
tagactttg aaactaagg aactccacg aactcgtca 
tcgtagcca aagaccatg gtttacaac cgctagaaa 
ccaccgctt tcgtctcaa tcagagtaa cagacgtca 
atgataatc agcccacaa tcaacgtca ttttcttga 
gcgatgatg ctactccga aaatcagcg aatcggcac 
aacctggtg aattcgtag cgaggattt ctgtcaacg 
atcccgatt ccaaaacgg aatactcca acataaatg 
tcacactga gtggaggtg acggccatt tcgggttga 
ccacaagag ataacatca tttatttgg taatagtgt 
ctttgcctc tggacaggg aattgcagc cgaattaca 
ctttggatg tggtcgcca ccgatcggg gaaaccccc 
cgctccacg cctccagga ccataaatc tgatggtga 
cggacatta acgacaacg cgcccgaat tcgaccaag 
aagtgtacc aagcaaacg tgatggaag tgtccgatc 
ctggaactt ccgtgatcc aggtcttgg ccaccgaca 
aggacgagg gcaacaaca gtgcgatca gctactcgc 
tgctggaca ccgtcgaga cgcattcgt cctggttcc 
agatcgacc ctcgcagtg gtttggtga cgacccgcg 
ctcacgtcg attgtgaaa cggatccgg tgccgcgtc 
tgaccgtag ttgctacag ataacggtt ttccgccgt 
tgtcgtctt cagcgacag tgctagtga ccatccacg 
acgtcaacg acaacgagc ccatcttcg accagagtt 
tctacaatg tttcggtgg ccgaaaacg aggccgaag 
gacgctgta ttctaaagg taaggaaaa acggcggcc 
gaggtggtg ggaatcctc catggaacg tctagtaaa 
aacgcgtcg atcaaaaac gaccaaaca aaagtaaaa 
gtttaaccg cgaatgaca ctgttaatt ggacaggag 
gtatgacac ggtgggtta ccgcgaacg gacgagata 
tgcgacata tttcaagtg tttgcattg ctgacaaca 
cgcagccga tgacgacgt taatggctt cacgaccag 
ggttttctc ggcgatcga tatttcgac ggcgacgac 
ggccaccca tcgagggtg ctcgcgacc ggctcgact 
tcgaggacc agcgtttcc taatataaa atttattaa 
gtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY