General information | |||
TC032879
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 8014182..8018932 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC032879 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07224 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032879 RA
MLLLLLLAA SRAEHVRDL EVSEGAPVG TRIGFIGDG ASDSGPPYL IVPVGSAVD SDLNIDQTT GEIRTKVPL DRETRSSYS LVAIPIRGD NVKVVVKVL DENDNAPTF PTSVMSIEF PENTPRDVK RTLHPARDL DLDIFNTQR YNIVSGNIN NTFRLSSHR ERDGVLYLD LQINGFLDR ETTPAYSLV IEALDGGTP PLRGEMRVN ITIQDVNDN QPIFNQSRY IATVPENAT VGTTVLQVF ATDKDSGEN GQIEYSINR RQSDRDNMF RIDPTTGLI VVNKPLDFE TKELHELVI VAKDHGLQP LETTAFVSI RVTDVNDNQ PTINVIFLS DDATPKISE SAQPGEFVA RISVNDPDS KTEYSNINV TLSGGDGHF GLTTRDNII YLVIVSLPL DRELQPNYT LDVVATDRG NPPLHASRT INLMVTDIN DNAPEFDQE VYQANVMEV SDPGTSVIQ VLATDKDEG NNSAISYSL LDTVETHSS WFQIDPRSG LVTTRAHVD CETDPVPRL TVVATDNGF PPLSSSATV LVTIHDVND NEPIFDQSF YNVSVAENE AEGRCILKV RKNGGRGGG NPPWNV
- >TC032879 RA
atgctgctg ctcctgctc ctggccgcg tccagggcg gagcacgtg cgcgacctc gaggtctcc gaaggggcg cccgtcggc accaggatt ggattcata ggtgatggg gcttccgac agcgggccg ccctatctc atcgttccg gtgggaagt gccgtcgat tcggacctc aacatcgac caaacgacg ggggaaata cggactaaa gtgcctttg gatcgcgaa acgcggtcg agttattcc cttgtggcc atccctatc agaggtgac aatgtgaaa gtggttgtt aaagtgctc gatgaaaac gacaacgct cccacgttt ccgacgtcg gttatgagc atcgagttc cccgaaaac acgccgaga gacgtcaaa cggactcta cacccggcg agagaccta gatcttgac attttcaac acacaaaga tacaacatt gtttcgggg aatatcaac aacacgttc aggttatcg tcacacagg gaacgcgac ggagtgctt tatttagac ctgcagatt aacgggttc ttagataga gaaacaacg ccagcttat agcctcgtt atagaggca ttagatggt ggaactcct cctctcaga ggcgagatg agggtcaat ataacgatc caggatgtt aatgataat caaccgatt tttaatcaa agtagatac attgcgacc gttcccgaa aacgccacc gttggcaca actgtcctc caggtcttc gccaccgat aaagattcc ggagagaac ggtcaaatc gaatattcg atcaatcgg agacaaagt gatcgagat aacatgttt agaatcgat ccgacaacc ggtctcatc gtagtcaac aaaccctta gactttgaa actaaggaa ctccacgaa ctcgtcatc gtagccaaa gaccatggt ttacaaccg ctagaaacc accgctttc gtctcaatc agagtaaca gacgtcaat gataatcag cccacaatc aacgtcatt ttcttgagc gatgatgct actccgaaa atcagcgaa tcggcacaa cctggtgaa ttcgtagcg aggatttct gtcaacgat cccgattcc aaaacggaa tactccaac ataaatgtc acactgagt ggaggtgac ggccatttc gggttgacc acaagagat aacatcatt tatttggta atagtgtct ttgcctctg gacagggaa ttgcagccg aattacact ttggatgtg gtcgccacc gatcgggga aaccccccg ctccacgcc tccaggacc ataaatctg atggtgacg gacattaac gacaacgcg cccgaattc gaccaagaa gtgtaccaa gcaaacgtg atggaagtg tccgatcct ggaacttcc gtgatccag gtcttggcc accgacaag gacgagggc aacaacagt gcgatcagc tactcgctg ctggacacc gtcgagacg cattcgtcc tggttccag atcgaccct cgcagtggt ttggtgacg acccgcgct cacgtcgat tgtgaaacg gatccggtg ccgcgtctg accgtagtt gctacagat aacggtttt ccgccgttg tcgtcttca gcgacagtg ctagtgacc atccacgac gtcaacgac aacgagccc atcttcgac cagagtttc tacaatgtt tcggtggcc gaaaacgag gccgaagga cgctgtatt ctaaaggta aggaaaaac ggcggccga ggtggtggg aatcctcca tggaacgtc tag
- >TC032879 RA
gtgctctgt accttcgcg cgattaatc atttcgatc taatataag ttgtaattt gtattgcca gccaaagat ggaatattg caaggctcg agctcagtc catctcaag accgaagcc gaagagtcg gtccgcgtc cttttcgct gtttcgcca gtcaccgcg tcctagtgc gacggcgac gccgtctcc ggagtgcca gtgatctcg agtgagtgc gcgccccga caccgaagc gcccggagt cgacgactc ctcctcctc tctttaaaa cctccggag agaacacgt cgtccgggt cgcaacgcg taactagcc gtcgttcgt gcacccgtt taatccccg gaacggcac cttcaaaag aatccggta aattgagga accattaca aacatattt agagagcga gcgcgcacc aacggcccc gtcgcatgt tttcaggcc cgctagtag cgcttttgt tctggttat tagcagctc catatcaag ggatgcacc agccgtcca ggccgctcc accaggatg cgtgaacgt ccgaaatga cgctgtcga cgatgctgc tgctcctgc tcctggccg cgtccaggg cggagcacg tgcgcgacc tcgaggtct ccgaagggg cgcccgtcg gcaccagga ttggattca taggtgatg gggcttccg acagcgggc cgccctatc tcatcgttc cggtgggaa gtgccgtcg attcggacc tcaacatcg accaaacga cgggggaaa tacggacta aagtgcctt tggatcgcg aaacgcggt cgagttatt cccttgtgg ccatcccta tcagaggtg acaatgtga aagtggttg ttaaagtgc tcgatgaaa acgacaacg ctcccacgt ttccgacgt cggttatga gcatcgagt tccccgaaa acacgccga gagacgtca aacggactc tacacccgg cgagagacc tagatcttg acattttca acacacaaa gatacaaca ttgtttcgg ggaatatca acaacacgt tcaggttat cgtcacaca gggaacgcg acggagtgc tttatttag acctgcaga ttaacgggt tcttagata gagaaacaa cgccagctt atagcctcg ttatagagg cattagatg gtggaactc ctcctctca gaggcgaga tgagggtca atataacga tccaggatg ttaatgata atcaaccga tttttaatc aaagtagat acattgcga ccgttcccg aaaacgcca ccgttggca caactgtcc tccaggtct tcgccaccg ataaagatt ccggagaga acggtcaaa tcgaatatt cgatcaatc ggagacaaa gtgatcgag ataacatgt ttagaatcg atccgacaa ccggtctca tcgtagtca acaaaccct tagactttg aaactaagg aactccacg aactcgtca tcgtagcca aagaccatg gtttacaac cgctagaaa ccaccgctt tcgtctcaa tcagagtaa cagacgtca atgataatc agcccacaa tcaacgtca ttttcttga gcgatgatg ctactccga aaatcagcg aatcggcac aacctggtg aattcgtag cgaggattt ctgtcaacg atcccgatt ccaaaacgg aatactcca acataaatg tcacactga gtggaggtg acggccatt tcgggttga ccacaagag ataacatca tttatttgg taatagtgt ctttgcctc tggacaggg aattgcagc cgaattaca ctttggatg tggtcgcca ccgatcggg gaaaccccc cgctccacg cctccagga ccataaatc tgatggtga cggacatta acgacaacg cgcccgaat tcgaccaag aagtgtacc aagcaaacg tgatggaag tgtccgatc ctggaactt ccgtgatcc aggtcttgg ccaccgaca aggacgagg gcaacaaca gtgcgatca gctactcgc tgctggaca ccgtcgaga cgcattcgt cctggttcc agatcgacc ctcgcagtg gtttggtga cgacccgcg ctcacgtcg attgtgaaa cggatccgg tgccgcgtc tgaccgtag ttgctacag ataacggtt ttccgccgt tgtcgtctt cagcgacag tgctagtga ccatccacg acgtcaacg acaacgagc ccatcttcg accagagtt tctacaatg tttcggtgg ccgaaaacg aggccgaag gacgctgta ttctaaagg taaggaaaa acggcggcc gaggtggtg ggaatcctc catggaacg tctagtaaa aacgcgtcg atcaaaaac gaccaaaca aaagtaaaa gtttaaccg cgaatgaca ctgttaatt ggacaggag gtatgacac ggtgggtta ccgcgaacg gacgagata tgcgacata tttcaagtg tttgcattg ctgacaaca cgcagccga tgacgacgt taatggctt cacgaccag ggttttctc ggcgatcga tatttcgac ggcgacgac ggccaccca tcgagggtg ctcgcgacc ggctcgact tcgaggacc agcgtttcc taatataaa atttattaa gtg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
