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Gene information
General information
TC007194 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7793421..7814693
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007194
    iBeetle screen iB_07222

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    antenna antennal flagellum number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna scapus structure not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: Stigma missing
    gena triplet bristle number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007194 RA
      MLISNGNAL GSGNDFSNV PGLSGCCPV PVAPAARSA 
      IVNWKMDSV GLPAPNTAP SVLSTAIPL SPTVASSPT 
      ITSPAGAAS GASTVPSSL APSALRELY GNAATAPGH 
      PLASLVSQQ RFLELSRFG LRHYDLAQH MLTQQGAVT 
      KLLGTLRPP GLIGGSKPK VATPAVVSK IEQYKRENP 
      TIFAWEIRE RLISEGVCT NATAPSVSS INRILRNRA 
      AERAAAEFA RAAGYGLYH PHPYAAAAA AFPWHSPLW 
      PNSPLGLQP PGAGSSMMA GSPSSSGSP HPDHGLLNS 
      PPGTHPDSK DDSSLDSSE QPKFRRNRT TFSPDQLEE 
      LEKEFEKSH YPCVSTRER LASKTSLSE ARVQVWFSN 
      RRAKWRRHQ RMNLLKQSS PGPQGSQSS MFGGRLTPS 
      PHYTAASTP GSPLTTRTG GPTASSLLL QMGGENSAF 
      KALVPNQLL AHDNMRRLS DYATDSEEE INVNDESDN 
      DEPRRRRSR SSSPVEVVD SPAKETPTC QPLQLTMHD 
      RD
    CDS sequence
    • >TC007194 RA
      atgctaatc tccaacgga aacgcgttg ggaagcgga 
      aacgacttt tccaatgtt cctggactt tccggctgt 
      tgtcccgtt ccggtcgct cccgccgcc cgatccgcc 
      attgtcaac tggaaaatg gactccgtc gggttgcct 
      gcccctaac acggcgccc agtgttctc tctacggcc 
      attccgctc tcgcccaca gtagcctca tcgccgaca 
      attaccagt cctgctggg gccgcgtcg ggagcctcg 
      acggtcccg tcctccctt gccccatct gccctcagg 
      gagctgtac ggaaacgcg gccacggct cccggccat 
      ccgctcgct tcgctagtc tcgcaacaa aggttcctg 
      gagttgtcc agattcggg ctaagacac tacgacctg 
      gcccagcat atgctcacc caacaaggt gccgtcacg 
      aaattgttg ggcacgctt cgaccgcct ggactaatc 
      ggtggcagc aagcccaaa gtggcaact ccagccgta 
      gtctcgaag atagaacag tacaaaaga gagaatccg 
      acgattttc gcatgggaa atccgagag agattgata 
      tcagaaggt gtgtgcact aatgcgacg gctccatcc 
      gtgagtagc atcaacagg atactcaga aatcgcgcg 
      gcggaaaga gctgccgcg gaattcgcc cgagctgct 
      ggctatggc ctctaccac ccccatcct tacgcagca 
      gctgctgct gccttccct tggcattca cctctgtgg 
      cctaacagt cctctggga ctccaacct ccaggcgcc 
      ggttcgtcc atgatggcc ggctccccc agctcctct 
      ggatcccca catcccgac cacggcctt ctcaactca 
      cctcctggc acccacccc gacagcaaa gacgattcg 
      agtctggac agctccgag caacccaaa tttagaaga 
      aaccggaca actttcagc cccgatcag ttggaagag 
      ctggaaaag gagttcgag aagagccac tatccgtgc 
      gtttcgacg cgggagcgt ctcgcgtcg aagacttcg 
      ctgtcggag gcccgggtt caggtttgg ttttctaac 
      agacgagcg aaatggaga cgccaccag cgcatgaac 
      ctgctaaag cagtcctct ccgggcccg caaggcagc 
      cagtcttcg atgttcggc ggccgcttg acgccctca 
      ccgcactac acagccgct tccacccct ggctcgccg 
      cttactact cgcaccggg ggaccgacg gcttcctcc 
      cttttgttg cagatgggc ggagaaaat agtgccttc 
      aaagctttg gtaccgaac caattgctg gcgcatgat 
      aacatgaga cgtctcagt gattacgcg acggattcc 
      gaggaggag atcaacgtc aacgacgag tcggataac 
      gacgagcca cggaggcgg aggtcgcgc tcttcgagt 
      cccgtcgaa gtagtcgac tcaccggcc aaagagacg 
      cccacatgt cagcctttg cagctcact atgcacgat 
      cgcgattaa
    mRNA sequence
    • >TC007194 RA
      aaaggtaac caaagcagt gggcagagc acaggaggg 
      ttcaagcct gcctatcgt gtgagcaca agtagccaa 
      ccgagctcg agtcgatga gcttcaacc tcttcacca 
      gaggagagc ctccaaggt ttctggcgc gcagtgaac 
      ttgaaacgc ggtgataac attaacgag tgatcgtgt 
      gcagagagg cacccatga acagtgcgc cagcaaaat 
      gctaatctc caacggaaa cgcgttggg aagcggaaa 
      cgacttttc caatgttcc tggactttc cggctgttg 
      tcccgttcc ggtcgctcc cgccgcccg atccgccat 
      tgtcaactg gaaaatgga ctccgtcgg gttgcctgc 
      ccctaacac ggcgcccag tgttctctc tacggccat 
      tccgctctc gcccacagt agcctcatc gccgacaat 
      taccagtcc tgctggggc cgcgtcggg agcctcgac 
      ggtcccgtc ctcccttgc cccatctgc cctcaggga 
      gctgtacgg aaacgcggc cacggctcc cggccatcc 
      gctcgcttc gctagtctc gcaacaaag gttcctgga 
      gttgtccag attcgggct aagacacta cgacctggc 
      ccagcatat gctcaccca acaaggtgc cgtcacgaa 
      attgttggg cacgcttcg accgcctgg actaatcgg 
      tggcagcaa gcccaaagt ggcaactcc agccgtagt 
      ctcgaagat agaacagta caaaagaga gaatccgac 
      gattttcgc atgggaaat ccgagagag attgatatc 
      agaaggtgt gtgcactaa tgcgacggc tccatccgt 
      gagtagcat caacaggat actcagaaa tcgcgcggc 
      ggaaagagc tgccgcgga attcgcccg agctgctgg 
      ctatggcct ctaccaccc ccatcctta cgcagcagc 
      tgctgctgc cttcccttg gcattcacc tctgtggcc 
      taacagtcc tctgggact ccaacctcc aggcgccgg 
      ttcgtccat gatggccgg ctcccccag ctcctctgg 
      atccccaca tcccgacca cggccttct caactcacc 
      tcctggcac ccaccccga cagcaaaga cgattcgag 
      tctggacag ctccgagca acccaaatt tagaagaaa 
      ccggacaac tttcagccc cgatcagtt ggaagagct 
      ggaaaagga gttcgagaa gagccacta tccgtgcgt 
      ttcgacgcg ggagcgtct cgcgtcgaa gacttcgct 
      gtcggaggc ccgggttca ggtttggtt ttctaacag 
      acgagcgaa atggagacg ccaccagcg catgaacct 
      gctaaagca gtcctctcc gggcccgca aggcagcca 
      gtcttcgat gttcggcgg ccgcttgac gccctcacc 
      gcactacac agccgcttc cacccctgg ctcgccgct 
      tactactcg caccggggg accgacggc ttcctccct 
      tttgttgca gatgggcgg agaaaatag tgccttcaa 
      agctttggt accgaacca attgctggc gcatgataa 
      catgagacg tctcagtga ttacgcgac ggattccga 
      ggaggagat caacgtcaa cgacgagtc ggataacga 
      cgagccacg gaggcggag gtcgcgctc ttcgagtcc 
      cgtcgaagt agtcgactc accggccaa agagacgcc 
      cacatgtca gcctttgca gctcactat gcacgatcg 
      cgattaatt tacgattac gtacatggt gaaacaaca 
      ttaccaaag caattcaac tcttataac acttattta 
      tctctgtaa atactattt aaatgacgc gacggatac 
      accaaaagg ttcctccac tcggcccga taattaaca 
      cagatggca gttttgttc caaaatcaa aaaagactt 
      aagctcact attcagtat tagcgaatt aagaagtca 
      aaatttttg gtagcaagt caggtcagg tcaaattat 
      ttcaaaccc ctactagtc atttcttgt tttgatttt 
      ggaaccgtt gtcgccggt gacccgagt ccctcacct 
      agtgttggt ttcgtagga gtaatctga tgcgaggat 
      catagaaat aactggacg cacttgtaa ataatagaa 
      gaatgagtc gtactgtct gaacgtact tgtagaata 
      ggaggaaat tgttgatat ttaaattat gtgtatatg 
      gaagacgag gaaatcgtc ttggcttaa taaattgcc 
      tggttgtcg tgttgttag agagcgtag gcttttcta 
      atgaatgga cagccgcgg caactcgtc cattgtata 
      gtagccgta agaaactgt aataattgt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY