Status
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Gene information
General information
TC007200 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7630812..7639863
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007200
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment potentially number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007200 RA
      MRTRSNSGV RLDYYHRLV HKIIMSNQN PVTGLFPAS 
      LENSHAWIR DNVYCIFAV WGLSMSYKK MADQDEDRA 
      KTYELEQSC VKLMRGLLM AMMQQKEKV ERFKQTQNP 
      LDSLHAKYS SQTGQIVVG DNEWGHLQI DAISLYLLI 
      LAQMTASGL QIVFNLDEV AFIQNLVFY IECAYCTPD 
      YGIWERGDK TNHGLPELN ASSIGMAKA ALEAMNELD 
      LFGARGGPY SIIHVLADE AQKCQAVLQ SMLPRESSS 
      KEIDSGLLS VVSFPAFAV DDPDLLQYT KDQIIEKLQ 
      GRYGCKRFL RDGYKTPKE DPRRLYYEP WELRMFENI 
      ECEWPLFFC YLIIENCFR GNKQGAAEY TQQLENIMI 
      RTEDGLRYV PELYAVPQE LVEAEYKEP GTQKRVALG 
      HCPFLWAQS LYIVGKLLQ EGFLAPGEL DPLNRRLCS 
      EKKPDVVVQ VVILAEDNE IRDKLAEHD IMVQTISEV 
      APIEVQPAR VLSHLYTYL GRNKKLGLS GRKSRDVGI 
      LSTSKLYSL GDKIFAFTP QFTDMSHNY IASDYELMI 
      DICKSEINF LKSSWQNML GRPLVTIAC RKLHLDDGK 
      IPLAMITTM KKLKSGYIN GTRVTLGHL NDFLSTSCI 
      TNLSFLGCH EDGLPDKLN PQVHQYLEE HLVKSLSSK 
      SSLLKQTHG RTTRKHMRR RMSVRGAIK KTRSINVDS 
      ETLGMEGNT TIERRGSMF LTNWQDTVD SASPLEHVR 
      SPSPENAPQ VPKDLQRKN IPNINVTRH KTNSETQYA 
      DTEVEELFA MLRESESLD EQGDILQYL VDSKGLDFH 
      TGMLDFGKP ITVRDLLKG LYEKACQQK MWGLVRHTA 
      GMLGKRVED LAKSVTDLL VRQKQITVG MPPHNEHTI 
      TAPLPESEL RLLIHDAYG EDDSTAMLT QELLVYLAM 
      FIRTEPQLF LEMLRLRVG LIIQVMATE LSRTLICDG 
      DEASEHLLN LSPFEMKNL LHHIISGKE FAISSVGRG 
      NFSIVSNKS SRISKKSHI SLGQDSNDE MIEGDRQGQ 
      WLRRRRLDG ALNRVPRDF YPRVWSVLE RCQGIDIAG 
      KVLPQSLTQ EMTSGELKF ALAVETVLN TIPQPEYRQ 
      LIVEALMVL TLVAEYNIA PSLGGIIQV EHLVHIANE 
      LFLDDQMKC DGDATLCCA KPKEMRETS KTGGLLCGG 
      AAYICQHFY DSAPSGCYG TMTYMTRAV AVTLDCLPK 
      QGELDCTIS
    CDS sequence
    • >TC007200 RA
      atgcgtact aggagcaat tccggtgtc cgcctggac 
      tattaccac cgactcgtc cacaaaata atcatgtcg 
      aaccaaaac ccggtcacg gggctgttt ccagcaagt 
      ttggagaac agccacgcc tggatccgc gacaacgtc 
      tattgcatc ttcgcggtg tggggcctt tcaatgtct 
      tacaaaaaa atggctgat caggacgaa gaccgggcc 
      aagacttac gagctggaa cagtcctgt gtcaaacta 
      atgcggggc cttttaatg gcaatgatg caacagaag 
      gaaaaggtc gagcgtttc aagcaaacc caaaacccg 
      cttgattcc ctccacgcc aaatactcg tcccaaacg 
      ggccagatc gtggtcggt gacaacgaa tgggggcac 
      ttgcagatc gatgccatt tcgctgtat ttgttgatt 
      ctggcgcaa atgacggca tcgggcctc cagatcgtg 
      tttaatcta gacgaggtc gcgtttatt caaaatttg 
      gtgttttat attgagtgt gcctattgc acccccgat 
      tacggtatt tgggaacgt ggggataaa acgaaccac 
      ggtttaccg gagctcaat gcgagttcc atcggcatg 
      gcgaaggct gcgcttgag gccatgaac gagttggat 
      ttatttggg gcccgaggg gggccttac agcataata 
      cacgttttg gccgatgag gcccaaaag tgccaagcg 
      gttttacaa tcaatgctc ccacgggag tcaagttca 
      aaggaaata gacagcggg ttgttgtcg gttgttagc 
      tttcctgct tttgcagtg gatgatcct gatttactc 
      caatacact aaggatcaa attattgaa aagttgcaa 
      ggtcgatac gggtgtaag cggtttttg cgggatggc 
      tacaagaca ccgaaagag gacccccga aggctgtac 
      tacgagcct tgggagctc cgcatgttc gaaaacatc 
      gaatgcgaa tggccgtta tttttttgt tacctaatc 
      atcgaaaat tgcttcagg ggtaacaag caaggagcg 
      gctgaatac acccaacaa ctcgaaaat atcatgata 
      cgcactgag gacggcttg agatacgtc ccggaatta 
      tatgcagtt ccgcaagaa ctggtagaa gcggagtat 
      aaggagcca gggacccag aaaagggtg gctttggga 
      cactgtccc tttctgtgg gcccaatct ctttacatc 
      gttggtaag ctgttgcaa gagggattt ctagctcct 
      ggcgaactg gatccccta aaccgtcgt ctctgttcg 
      gagaaaaaa ccggacgtg gtggtccaa gtcgtgatt 
      ttggccgaa gacaacgaa attagggat aaactcgca 
      gagcacgac attatggtg caaacaatc agcgaagtg 
      gcccccatt gaagtgcaa ccagctcgg gttttgtca 
      catttgtac acttatctc gggagaaac aaaaagttg 
      ggcttgtcg gggcgtaaa tcccgcgac gttgggatt 
      ctcagcact agcaaactg tattcattg ggggacaaa 
      atcttcgcg ttcacgcca cagtttacg gacatgtct 
      cacaattac atagcgagc gactatgaa ctgatgata 
      gatatttgc aagagcgaa ataaatttc ttgaagtca 
      agttggcag aatatgttg ggccggcca cttgtaaca 
      attgcgtgt cggaaacta catttagac gatggaaaa 
      atcccccta gctatgatc accacaatg aagaaactc 
      aaatcgggt tacatcaac ggcacgaga gtcactcta 
      ggccacctc aacgatttt ctcagcacg agctgcatc 
      actaatttg agcttcctg gggtgtcat gaggacggc 
      ttacccgac aaattgaac ccccaagtc caccaatac 
      ctcgaggag cacttggtg aagtcactc tcaagcaaa 
      tcgtcgctt cttaaacaa acccacggc agaaccacc 
      agaaaacac atgcgacgg cgaatgtca gtgcgtggc 
      gcaatcaag aaaacaagg tcaatcaac gtcgattcc 
      gagacactg ggcatggaa ggaaacaca accattgaa 
      cgtcgcggt tcaatgttc ctcaccaac tggcaagac 
      accgtcgac agtgcatcg ccactcgaa cacgtgcga 
      tccccatcc cccgaaaac gccccccag gtccccaaa 
      gacttgcaa cgcaaaaac atccccaac ataaacgtg 
      acgcgccac aaaaccaac tccgaaacg caatacgcc 
      gacaccgag gtcgaggag ttgttcgca atgttgcgc 
      gagtctgag tctctggat gaacaaggc gacatcctc 
      cagtacctt gtcgactcc aaagggctg gatttccac 
      actgggatg ctcgatttc gggaaaccg atcacagtg 
      cgcgatttg ctcaaaggc ttgtatgag aaggcgtgc 
      cagcagaag atgtggggc ttggtcaga cacacggcc 
      gggatgttg gggaaacga gtcgaagat ttggccaaa 
      tcggtgact gatttacta gtgcgtcag aagcaaatt 
      acagtgggg atgccccca cacaacgag catacgatc 
      acggccccc ttgccggaa agcgagttg agactgttg 
      attcatgac gcttatggc gaggacgac agtacggca 
      atgctgacg caagagttg ttagtctac cttgccatg 
      ttcataaga actgagcca caattgttt ttggaaatg 
      ttgagatta cgagtcggg ttgataatt caagtcatg 
      gcaacggaa ttatcccga acgctgatt tgcgacggg 
      gacgaagcg tccgagcat ttgcttaat ctgagtcct 
      tttgaaatg aaaaatttg ctacatcac ataatcagt 
      gggaaggaa tttgcaatc agtagcgtt ggtagaggg 
      aatttctcg attgttagc aacaaatca agccgaatt 
      agtaaaaaa agtcatatc agtttaggt caagacagc 
      aatgatgag atgatagag ggggacagg caagggcag 
      tggttaaga cggcgccgg ctggatggg gccttaaat 
      agggtcccc cgtgatttc tacccccgg gtttggagt 
      gtcttagaa cggtgtcag ggtatagac atcgcgggc 
      aaagtcttg ccgcaaagc ctgacgcaa gagatgacc 
      tctggtgag ttaaaattc gctttagcg gtcgaaacg 
      gtcttgaac acaatccca caacccgaa taccgccaa 
      ttaatagtt gaagctttg atggttcta actttggtg 
      gcggaatac aacatagcg ccgtctcta ggcgggatt 
      atccaagtt gaacattta gtccacatc gctaatgaa 
      ttatttctc gacgatcag atgaaatgt gacggtgat 
      gccacatta tgttgtgcc aaacccaaa gaaatgagg 
      gaaacgtcc aaaactgga ggcttgtta tgtggcggc 
      gctgcatac atttgccaa catttttac gacagcgct 
      ccgagcggc tgttatggg actatgacc tacatgacg 
      agagcagtg gctgtgact ttggactgt cttccaaaa 
      caaggagaa ctcgattgc acaattagt taa
    mRNA sequence
    • >TC007200 RA
      atgtctttg tggtgtgtc tttaattaa gtggtagtt 
      attgtgagt tgagttcgt tcaaaatgc gtactagga 
      gcaattccg gtgtccgcc tggactatt accaccgac 
      tcgtccaca aaataatca tgtcgaacc aaaacccgg 
      tcacggggc tgtttccag caagtttgg agaacagcc 
      acgcctgga tccgcgaca acgtctatt gcatcttcg 
      cggtgtggg gcctttcaa tgtcttaca aaaaaatgg 
      ctgatcagg acgaagacc gggccaaga cttacgagc 
      tggaacagt cctgtgtca aactaatgc ggggccttt 
      taatggcaa tgatgcaac agaaggaaa aggtcgagc 
      gtttcaagc aaacccaaa acccgcttg attccctcc 
      acgccaaat actcgtccc aaacgggcc agatcgtgg 
      tcggtgaca acgaatggg ggcacttgc agatcgatg 
      ccatttcgc tgtatttgt tgattctgg cgcaaatga 
      cggcatcgg gcctccaga tcgtgttta atctagacg 
      aggtcgcgt ttattcaaa atttggtgt tttatattg 
      agtgtgcct attgcaccc ccgattacg gtatttggg 
      aacgtgggg ataaaacga accacggtt taccggagc 
      tcaatgcga gttccatcg gcatggcga aggctgcgc 
      ttgaggcca tgaacgagt tggatttat ttggggccc 
      gaggggggc cttacagca taatacacg ttttggccg 
      atgaggccc aaaagtgcc aagcggttt tacaatcaa 
      tgctcccac gggagtcaa gttcaaagg aaatagaca 
      gcgggttgt tgtcggttg ttagctttc ctgcttttg 
      cagtggatg atcctgatt tactccaat acactaagg 
      atcaaatta ttgaaaagt tgcaaggtc gatacgggt 
      gtaagcggt ttttgcggg atggctaca agacaccga 
      aagaggacc cccgaaggc tgtactacg agccttggg 
      agctccgca tgttcgaaa acatcgaat gcgaatggc 
      cgttatttt tttgttacc taatcatcg aaaattgct 
      tcaggggta acaagcaag gagcggctg aatacaccc 
      aacaactcg aaaatatca tgatacgca ctgaggacg 
      gcttgagat acgtcccgg aattatatg cagttccgc 
      aagaactgg tagaagcgg agtataagg agccaggga 
      cccagaaaa gggtggctt tgggacact gtccctttc 
      tgtgggccc aatctcttt acatcgttg gtaagctgt 
      tgcaagagg gatttctag ctcctggcg aactggatc 
      ccctaaacc gtcgtctct gttcggaga aaaaaccgg 
      acgtggtgg tccaagtcg tgattttgg ccgaagaca 
      acgaaatta gggataaac tcgcagagc acgacatta 
      tggtgcaaa caatcagcg aagtggccc ccattgaag 
      tgcaaccag ctcgggttt tgtcacatt tgtacactt 
      atctcggga gaaacaaaa agttgggct tgtcggggc 
      gtaaatccc gcgacgttg ggattctca gcactagca 
      aactgtatt cattggggg acaaaatct tcgcgttca 
      cgccacagt ttacggaca tgtctcaca attacatag 
      cgagcgact atgaactga tgatagata tttgcaaga 
      gcgaaataa atttcttga agtcaagtt ggcagaata 
      tgttgggcc ggccacttg taacaattg cgtgtcgga 
      aactacatt tagacgatg gaaaaatcc ccctagcta 
      tgatcacca caatgaaga aactcaaat cgggttaca 
      tcaacggca cgagagtca ctctaggcc acctcaacg 
      attttctca gcacgagct gcatcacta atttgagct 
      tcctggggt gtcatgagg acggcttac ccgacaaat 
      tgaaccccc aagtccacc aatacctcg aggagcact 
      tggtgaagt cactctcaa gcaaatcgt cgcttctta 
      aacaaaccc acggcagaa ccaccagaa aacacatgc 
      gacggcgaa tgtcagtgc gtggcgcaa tcaagaaaa 
      caaggtcaa tcaacgtcg attccgaga cactgggca 
      tggaaggaa acacaacca ttgaacgtc gcggttcaa 
      tgttcctca ccaactggc aagacaccg tcgacagtg 
      catcgccac tcgaacacg tgcgatccc catcccccg 
      aaaacgccc cccaggtcc ccaaagact tgcaacgca 
      aaaacatcc ccaacataa acgtgacgc gccacaaaa 
      ccaactccg aaacgcaat acgccgaca ccgaggtcg 
      aggagttgt tcgcaatgt tgcgcgagt ctgagtctc 
      tggatgaac aaggcgaca tcctccagt accttgtcg 
      actccaaag ggctggatt tccacactg ggatgctcg 
      atttcggga aaccgatca cagtgcgcg atttgctca 
      aaggcttgt atgagaagg cgtgccagc agaagatgt 
      ggggcttgg tcagacaca cggccggga tgttgggga 
      aacgagtcg aagatttgg ccaaatcgg tgactgatt 
      tactagtgc gtcagaagc aaattacag tggggatgc 
      ccccacaca acgagcata cgatcacgg cccccttgc 
      cggaaagcg agttgagac tgttgattc atgacgctt 
      atggcgagg acgacagta cggcaatgc tgacgcaag 
      agttgttag tctaccttg ccatgttca taagaactg 
      agccacaat tgtttttgg aaatgttga gattacgag 
      tcgggttga taattcaag tcatggcaa cggaattat 
      cccgaacgc tgatttgcg acggggacg aagcgtccg 
      agcatttgc ttaatctga gtccttttg aaatgaaaa 
      atttgctac atcacataa tcagtggga aggaatttg 
      caatcagta gcgttggta gagggaatt tctcgattg 
      ttagcaaca aatcaagcc gaattagta aaaaaagtc 
      atatcagtt taggtcaag acagcaatg atgagatga 
      tagaggggg acaggcaag ggcagtggt taagacggc 
      gccggctgg atggggcct taaataggg tcccccgtg 
      atttctacc cccgggttt ggagtgtct tagaacggt 
      gtcagggta tagacatcg cgggcaaag tcttgccgc 
      aaagcctga cgcaagaga tgacctctg gtgagttaa 
      aattcgctt tagcggtcg aaacggtct tgaacacaa 
      tcccacaac ccgaatacc gccaattaa tagttgaag 
      ctttgatgg ttctaactt tggtggcgg aatacaaca 
      tagcgccgt ctctaggcg ggattatcc aagttgaac 
      atttagtcc acatcgcta atgaattat ttctcgacg 
      atcagatga aatgtgacg gtgatgcca cattatgtt 
      gtgccaaac ccaaagaaa tgagggaaa cgtccaaaa 
      ctggaggct tgttatgtg gcggcgctg catacattt 
      gccaacatt tttacgaca gcgctccga gcggctgtt 
      atgggacta tgacctaca tgacgagag cagtggctg 
      tgactttgg actgtcttc caaaacaag gagaactcg 
      attgcacaa ttagttaat tatgtttta ttgttggag 
      gggtttatt gtttaataa aaaatagtt ctatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY