Status
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Gene information
General information
TC007200 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7630812..7639863
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007200
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment potentially number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007200 RA
      MRTRSNSGV RLDYYHRLV HKIIMSNQN PVTGLFPAS 
LENSHAWIR DNVYCIFAV WGLSMSYKK MADQDEDRA 
KTYELEQSC VKLMRGLLM AMMQQKEKV ERFKQTQNP 
LDSLHAKYS SQTGQIVVG DNEWGHLQI DAISLYLLI 
LAQMTASGL QIVFNLDEV AFIQNLVFY IECAYCTPD 
YGIWERGDK TNHGLPELN ASSIGMAKA ALEAMNELD 
LFGARGGPY SIIHVLADE AQKCQAVLQ SMLPRESSS 
KEIDSGLLS VVSFPAFAV DDPDLLQYT KDQIIEKLQ 
GRYGCKRFL RDGYKTPKE DPRRLYYEP WELRMFENI 
ECEWPLFFC YLIIENCFR GNKQGAAEY TQQLENIMI 
RTEDGLRYV PELYAVPQE LVEAEYKEP GTQKRVALG 
HCPFLWAQS LYIVGKLLQ EGFLAPGEL DPLNRRLCS 
EKKPDVVVQ VVILAEDNE IRDKLAEHD IMVQTISEV 
APIEVQPAR VLSHLYTYL GRNKKLGLS GRKSRDVGI 
LSTSKLYSL GDKIFAFTP QFTDMSHNY IASDYELMI 
DICKSEINF LKSSWQNML GRPLVTIAC RKLHLDDGK 
IPLAMITTM KKLKSGYIN GTRVTLGHL NDFLSTSCI 
TNLSFLGCH EDGLPDKLN PQVHQYLEE HLVKSLSSK 
SSLLKQTHG RTTRKHMRR RMSVRGAIK KTRSINVDS 
ETLGMEGNT TIERRGSMF LTNWQDTVD SASPLEHVR 
SPSPENAPQ VPKDLQRKN IPNINVTRH KTNSETQYA 
DTEVEELFA MLRESESLD EQGDILQYL VDSKGLDFH 
TGMLDFGKP ITVRDLLKG LYEKACQQK MWGLVRHTA 
GMLGKRVED LAKSVTDLL VRQKQITVG MPPHNEHTI 
TAPLPESEL RLLIHDAYG EDDSTAMLT QELLVYLAM 
FIRTEPQLF LEMLRLRVG LIIQVMATE LSRTLICDG 
DEASEHLLN LSPFEMKNL LHHIISGKE FAISSVGRG 
NFSIVSNKS SRISKKSHI SLGQDSNDE MIEGDRQGQ 
WLRRRRLDG ALNRVPRDF YPRVWSVLE RCQGIDIAG 
KVLPQSLTQ EMTSGELKF ALAVETVLN TIPQPEYRQ 
LIVEALMVL TLVAEYNIA PSLGGIIQV EHLVHIANE 
LFLDDQMKC DGDATLCCA KPKEMRETS KTGGLLCGG 
AAYICQHFY DSAPSGCYG TMTYMTRAV AVTLDCLPK 
QGELDCTIS
    CDS sequence
    • >TC007200 RA
      atgcgtact aggagcaat tccggtgtc cgcctggac 
tattaccac cgactcgtc cacaaaata atcatgtcg 
aaccaaaac ccggtcacg gggctgttt ccagcaagt 
ttggagaac agccacgcc tggatccgc gacaacgtc 
tattgcatc ttcgcggtg tggggcctt tcaatgtct 
tacaaaaaa atggctgat caggacgaa gaccgggcc 
aagacttac gagctggaa cagtcctgt gtcaaacta 
atgcggggc cttttaatg gcaatgatg caacagaag 
gaaaaggtc gagcgtttc aagcaaacc caaaacccg 
cttgattcc ctccacgcc aaatactcg tcccaaacg 
ggccagatc gtggtcggt gacaacgaa tgggggcac 
ttgcagatc gatgccatt tcgctgtat ttgttgatt 
ctggcgcaa atgacggca tcgggcctc cagatcgtg 
tttaatcta gacgaggtc gcgtttatt caaaatttg 
gtgttttat attgagtgt gcctattgc acccccgat 
tacggtatt tgggaacgt ggggataaa acgaaccac 
ggtttaccg gagctcaat gcgagttcc atcggcatg 
gcgaaggct gcgcttgag gccatgaac gagttggat 
ttatttggg gcccgaggg gggccttac agcataata 
cacgttttg gccgatgag gcccaaaag tgccaagcg 
gttttacaa tcaatgctc ccacgggag tcaagttca 
aaggaaata gacagcggg ttgttgtcg gttgttagc 
tttcctgct tttgcagtg gatgatcct gatttactc 
caatacact aaggatcaa attattgaa aagttgcaa 
ggtcgatac gggtgtaag cggtttttg cgggatggc 
tacaagaca ccgaaagag gacccccga aggctgtac 
tacgagcct tgggagctc cgcatgttc gaaaacatc 
gaatgcgaa tggccgtta tttttttgt tacctaatc 
atcgaaaat tgcttcagg ggtaacaag caaggagcg 
gctgaatac acccaacaa ctcgaaaat atcatgata 
cgcactgag gacggcttg agatacgtc ccggaatta 
tatgcagtt ccgcaagaa ctggtagaa gcggagtat 
aaggagcca gggacccag aaaagggtg gctttggga 
cactgtccc tttctgtgg gcccaatct ctttacatc 
gttggtaag ctgttgcaa gagggattt ctagctcct 
ggcgaactg gatccccta aaccgtcgt ctctgttcg 
gagaaaaaa ccggacgtg gtggtccaa gtcgtgatt 
ttggccgaa gacaacgaa attagggat aaactcgca 
gagcacgac attatggtg caaacaatc agcgaagtg 
gcccccatt gaagtgcaa ccagctcgg gttttgtca 
catttgtac acttatctc gggagaaac aaaaagttg 
ggcttgtcg gggcgtaaa tcccgcgac gttgggatt 
ctcagcact agcaaactg tattcattg ggggacaaa 
atcttcgcg ttcacgcca cagtttacg gacatgtct 
cacaattac atagcgagc gactatgaa ctgatgata 
gatatttgc aagagcgaa ataaatttc ttgaagtca 
agttggcag aatatgttg ggccggcca cttgtaaca 
attgcgtgt cggaaacta catttagac gatggaaaa 
atcccccta gctatgatc accacaatg aagaaactc 
aaatcgggt tacatcaac ggcacgaga gtcactcta 
ggccacctc aacgatttt ctcagcacg agctgcatc 
actaatttg agcttcctg gggtgtcat gaggacggc 
ttacccgac aaattgaac ccccaagtc caccaatac 
ctcgaggag cacttggtg aagtcactc tcaagcaaa 
tcgtcgctt cttaaacaa acccacggc agaaccacc 
agaaaacac atgcgacgg cgaatgtca gtgcgtggc 
gcaatcaag aaaacaagg tcaatcaac gtcgattcc 
gagacactg ggcatggaa ggaaacaca accattgaa 
cgtcgcggt tcaatgttc ctcaccaac tggcaagac 
accgtcgac agtgcatcg ccactcgaa cacgtgcga 
tccccatcc cccgaaaac gccccccag gtccccaaa 
gacttgcaa cgcaaaaac atccccaac ataaacgtg 
acgcgccac aaaaccaac tccgaaacg caatacgcc 
gacaccgag gtcgaggag ttgttcgca atgttgcgc 
gagtctgag tctctggat gaacaaggc gacatcctc 
cagtacctt gtcgactcc aaagggctg gatttccac 
actgggatg ctcgatttc gggaaaccg atcacagtg 
cgcgatttg ctcaaaggc ttgtatgag aaggcgtgc 
cagcagaag atgtggggc ttggtcaga cacacggcc 
gggatgttg gggaaacga gtcgaagat ttggccaaa 
tcggtgact gatttacta gtgcgtcag aagcaaatt 
acagtgggg atgccccca cacaacgag catacgatc 
acggccccc ttgccggaa agcgagttg agactgttg 
attcatgac gcttatggc gaggacgac agtacggca 
atgctgacg caagagttg ttagtctac cttgccatg 
ttcataaga actgagcca caattgttt ttggaaatg 
ttgagatta cgagtcggg ttgataatt caagtcatg 
gcaacggaa ttatcccga acgctgatt tgcgacggg 
gacgaagcg tccgagcat ttgcttaat ctgagtcct 
tttgaaatg aaaaatttg ctacatcac ataatcagt 
gggaaggaa tttgcaatc agtagcgtt ggtagaggg 
aatttctcg attgttagc aacaaatca agccgaatt 
agtaaaaaa agtcatatc agtttaggt caagacagc 
aatgatgag atgatagag ggggacagg caagggcag 
tggttaaga cggcgccgg ctggatggg gccttaaat 
agggtcccc cgtgatttc tacccccgg gtttggagt 
gtcttagaa cggtgtcag ggtatagac atcgcgggc 
aaagtcttg ccgcaaagc ctgacgcaa gagatgacc 
tctggtgag ttaaaattc gctttagcg gtcgaaacg 
gtcttgaac acaatccca caacccgaa taccgccaa 
ttaatagtt gaagctttg atggttcta actttggtg 
gcggaatac aacatagcg ccgtctcta ggcgggatt 
atccaagtt gaacattta gtccacatc gctaatgaa 
ttatttctc gacgatcag atgaaatgt gacggtgat 
gccacatta tgttgtgcc aaacccaaa gaaatgagg 
gaaacgtcc aaaactgga ggcttgtta tgtggcggc 
gctgcatac atttgccaa catttttac gacagcgct 
ccgagcggc tgttatggg actatgacc tacatgacg 
agagcagtg gctgtgact ttggactgt cttccaaaa 
caaggagaa ctcgattgc acaattagt taa
    mRNA sequence
    • >TC007200 RA
      atgtctttg tggtgtgtc tttaattaa gtggtagtt 
attgtgagt tgagttcgt tcaaaatgc gtactagga 
gcaattccg gtgtccgcc tggactatt accaccgac 
tcgtccaca aaataatca tgtcgaacc aaaacccgg 
tcacggggc tgtttccag caagtttgg agaacagcc 
acgcctgga tccgcgaca acgtctatt gcatcttcg 
cggtgtggg gcctttcaa tgtcttaca aaaaaatgg 
ctgatcagg acgaagacc gggccaaga cttacgagc 
tggaacagt cctgtgtca aactaatgc ggggccttt 
taatggcaa tgatgcaac agaaggaaa aggtcgagc 
gtttcaagc aaacccaaa acccgcttg attccctcc 
acgccaaat actcgtccc aaacgggcc agatcgtgg 
tcggtgaca acgaatggg ggcacttgc agatcgatg 
ccatttcgc tgtatttgt tgattctgg cgcaaatga 
cggcatcgg gcctccaga tcgtgttta atctagacg 
aggtcgcgt ttattcaaa atttggtgt tttatattg 
agtgtgcct attgcaccc ccgattacg gtatttggg 
aacgtgggg ataaaacga accacggtt taccggagc 
tcaatgcga gttccatcg gcatggcga aggctgcgc 
ttgaggcca tgaacgagt tggatttat ttggggccc 
gaggggggc cttacagca taatacacg ttttggccg 
atgaggccc aaaagtgcc aagcggttt tacaatcaa 
tgctcccac gggagtcaa gttcaaagg aaatagaca 
gcgggttgt tgtcggttg ttagctttc ctgcttttg 
cagtggatg atcctgatt tactccaat acactaagg 
atcaaatta ttgaaaagt tgcaaggtc gatacgggt 
gtaagcggt ttttgcggg atggctaca agacaccga 
aagaggacc cccgaaggc tgtactacg agccttggg 
agctccgca tgttcgaaa acatcgaat gcgaatggc 
cgttatttt tttgttacc taatcatcg aaaattgct 
tcaggggta acaagcaag gagcggctg aatacaccc 
aacaactcg aaaatatca tgatacgca ctgaggacg 
gcttgagat acgtcccgg aattatatg cagttccgc 
aagaactgg tagaagcgg agtataagg agccaggga 
cccagaaaa gggtggctt tgggacact gtccctttc 
tgtgggccc aatctcttt acatcgttg gtaagctgt 
tgcaagagg gatttctag ctcctggcg aactggatc 
ccctaaacc gtcgtctct gttcggaga aaaaaccgg 
acgtggtgg tccaagtcg tgattttgg ccgaagaca 
acgaaatta gggataaac tcgcagagc acgacatta 
tggtgcaaa caatcagcg aagtggccc ccattgaag 
tgcaaccag ctcgggttt tgtcacatt tgtacactt 
atctcggga gaaacaaaa agttgggct tgtcggggc 
gtaaatccc gcgacgttg ggattctca gcactagca 
aactgtatt cattggggg acaaaatct tcgcgttca 
cgccacagt ttacggaca tgtctcaca attacatag 
cgagcgact atgaactga tgatagata tttgcaaga 
gcgaaataa atttcttga agtcaagtt ggcagaata 
tgttgggcc ggccacttg taacaattg cgtgtcgga 
aactacatt tagacgatg gaaaaatcc ccctagcta 
tgatcacca caatgaaga aactcaaat cgggttaca 
tcaacggca cgagagtca ctctaggcc acctcaacg 
attttctca gcacgagct gcatcacta atttgagct 
tcctggggt gtcatgagg acggcttac ccgacaaat 
tgaaccccc aagtccacc aatacctcg aggagcact 
tggtgaagt cactctcaa gcaaatcgt cgcttctta 
aacaaaccc acggcagaa ccaccagaa aacacatgc 
gacggcgaa tgtcagtgc gtggcgcaa tcaagaaaa 
caaggtcaa tcaacgtcg attccgaga cactgggca 
tggaaggaa acacaacca ttgaacgtc gcggttcaa 
tgttcctca ccaactggc aagacaccg tcgacagtg 
catcgccac tcgaacacg tgcgatccc catcccccg 
aaaacgccc cccaggtcc ccaaagact tgcaacgca 
aaaacatcc ccaacataa acgtgacgc gccacaaaa 
ccaactccg aaacgcaat acgccgaca ccgaggtcg 
aggagttgt tcgcaatgt tgcgcgagt ctgagtctc 
tggatgaac aaggcgaca tcctccagt accttgtcg 
actccaaag ggctggatt tccacactg ggatgctcg 
atttcggga aaccgatca cagtgcgcg atttgctca 
aaggcttgt atgagaagg cgtgccagc agaagatgt 
ggggcttgg tcagacaca cggccggga tgttgggga 
aacgagtcg aagatttgg ccaaatcgg tgactgatt 
tactagtgc gtcagaagc aaattacag tggggatgc 
ccccacaca acgagcata cgatcacgg cccccttgc 
cggaaagcg agttgagac tgttgattc atgacgctt 
atggcgagg acgacagta cggcaatgc tgacgcaag 
agttgttag tctaccttg ccatgttca taagaactg 
agccacaat tgtttttgg aaatgttga gattacgag 
tcgggttga taattcaag tcatggcaa cggaattat 
cccgaacgc tgatttgcg acggggacg aagcgtccg 
agcatttgc ttaatctga gtccttttg aaatgaaaa 
atttgctac atcacataa tcagtggga aggaatttg 
caatcagta gcgttggta gagggaatt tctcgattg 
ttagcaaca aatcaagcc gaattagta aaaaaagtc 
atatcagtt taggtcaag acagcaatg atgagatga 
tagaggggg acaggcaag ggcagtggt taagacggc 
gccggctgg atggggcct taaataggg tcccccgtg 
atttctacc cccgggttt ggagtgtct tagaacggt 
gtcagggta tagacatcg cgggcaaag tcttgccgc 
aaagcctga cgcaagaga tgacctctg gtgagttaa 
aattcgctt tagcggtcg aaacggtct tgaacacaa 
tcccacaac ccgaatacc gccaattaa tagttgaag 
ctttgatgg ttctaactt tggtggcgg aatacaaca 
tagcgccgt ctctaggcg ggattatcc aagttgaac 
atttagtcc acatcgcta atgaattat ttctcgacg 
atcagatga aatgtgacg gtgatgcca cattatgtt 
gtgccaaac ccaaagaaa tgagggaaa cgtccaaaa 
ctggaggct tgttatgtg gcggcgctg catacattt 
gccaacatt tttacgaca gcgctccga gcggctgtt 
atgggacta tgacctaca tgacgagag cagtggctg 
tgactttgg actgtcttc caaaacaag gagaactcg 
attgcacaa ttagttaat tatgtttta ttgttggag 
gggtttatt gtttaataa aaaatagtt ctatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY