Status
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Gene information
General information
TC008266 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7540865..7552900
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008266
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07215

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber shape bent - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008266 RA
      MKRFQLWLT VSLLTKFET GSGVSLKQS SRAPQMNNH 
      QNISSLLDN LLRGYDNSV RPDFGGPPA VVEVDIMVR 
      SMGPISEVD MTYSMDCYF RQSWVDRRL AFPGDTNDT 
      LALSISMLA RIWKPDTYF YNGKQSYLH TITTPNKFV 
      RLYQDGRVL YSSRLTIKA GCPMNLEDF PMDIQRCPL 
      KFGSFGYTA QDVLYRWNA ARQVAIAED MKLSQFDLI 
      ATPAANHTD TIVNSDTKS AEYSMLLVS FHLQRHMGN 
      FLIQVYGPC VLLVVLSWV SFWLNREAT ADRVSLGIT 
      TVLTMTFLG LEARTDLPK VPYPTALDF FVFLSFSFI 
      FATIIQFAV VHYFTKYGS GECYFSSDF SSDSSSDEE 
      DWIESRENA PKEVSHSPV CKSSNYGSS NGGDPHFIE 
      VIPLSACSI PVASMRRTS QPWSNLSCI NRSQDDKNY 
      HASPNKSTG SQKSPCQSQ KHRKKKKKR TPRFNSVSK 
      IDRASRVVF PVLFVTINL FYWYSYLSR TKRIVNLNV 
      S
    CDS sequence
    • >TC008266 RA
      atgaagcgg tttcaactt tggctgacc gtttcgctg 
      ctaaccaag tttgaaacc ggctcaggt gtgtccctc 
      aaacaatct tcacgggcg ccacaaatg aacaatcac 
      cagaacatc tcgtcgctt ttggataat ctcctcaga 
      ggttacgac aacagcgtc cgtcctgat ttcggcggc 
      cctcctgcg gtcgtggag gtcgatatc atggtgcgg 
      agcatgggc cccatctcg gaggtggac atgacgtat 
      tcaatggat tgctacttc cggcagtcg tgggtcgac 
      cgacgatta gccttccca ggggatacc aacgatact 
      ctggccctt agtatctcg atgctggca aggatctgg 
      aagccggat acttatttc tacaatggc aagcagtcg 
      tatttgcac accatcaca actcccaac aagttcgtc 
      agactgtat caagacggg agggtgctt tattcgtcg 
      agattgacg attaaagcc ggatgtcca atgaatctg 
      gaggacttt ccgatggac atccagagg tgtccgctg 
      aaattcggc tcgttcgga tacacggct caggatgtc 
      ctctacagg tggaacgcc gcgagacaa gtggcgatt 
      gcagaagac atgaagctg tcgcagttc gacctcatc 
      gccaccccc gctgccaat cacaccgac accatcgtc 
      aacagcgat accaaatca gctgaatat tcaatgctt 
      cttgtcagt ttccacttg caacgccac atgggcaac 
      tttctcatc caggtgtac ggcccttgt gtccttctc 
      gtcgtcctc tcttgggtc tccttctgg ctgaaccgc 
      gaagccaca gctgaccgc gtctccctc ggcataacc 
      accgtatta acgatgaca tttctcggt ctggaagcg 
      agaaccgac ttgcccaaa gttccctac cccacagcc 
      ctcgatttc ttcgtcttc ctgtcattt tccttcatc 
      tttgcaact attattcag ttcgctgtg gtccactac 
      ttcacgaag tacggttcc ggcgagtgc tacttcagc 
      tcggatttc agttccgat tccagcagc gacgaagag 
      gattggatt gaaagcagg gaaaatgcg cccaaagag 
      gtgtcgcat tctccagtg tgcaaaagc tcaaattac 
      ggcagctcg aacgggggc gatccgcat ttcattgaa 
      gtgattccg ctgtcagcg tgttcgatt ccagtggcg 
      tcaatgagg cgcacgtca cagccttgg agcaatctc 
      tcctgtatc aaccgatcg caagacgat aaaaattat 
      cacgcgagt ccgaacaaa tcaactgga agccaaaaa 
      tcgccctgt caaagccaa aaacacaga aagaagaaa 
      aagaaaagg acgccgagg tttaactca gtttcgaaa 
      attgatcga gcttctaga gtagtgttt cccgtcctt 
      tttgtaacg attaatttg ttttattgg tattcgtac 
      ctgtcgagg accaagaga attgtaaat ctgaatgtt 
      tcgtaa
    mRNA sequence
    • >TC008266 RA
      cctcagcaa aattccatc cgaaaataa ttctttgtt 
      tcagtttga ggatgaagc ggtttcaac tttggctga 
      ccgtttcgc tgctaacca agtttgaaa ccggctcag 
      gtgtgtccc tcaaacaat cttcacggg cgccacaaa 
      tgaacaatc accagaaca tctcgtcgc ttttggata 
      atctcctca gaggttacg acaacagcg tccgtcctg 
      atttcggcg gccctcctg cggtcgtgg aggtcgata 
      tcatggtgc ggagcatgg gccccatct cggaggtgg 
      acatgacgt attcaatgg attgctact tccggcagt 
      cgtgggtcg accgacgat tagccttcc caggggata 
      ccaacgata ctctggccc ttagtatct cgatgctgg 
      caaggatct ggaagccgg atacttatt tctacaatg 
      gcaagcagt cgtatttgc acaccatca caactccca 
      acaagttcg tcagactgt atcaagacg ggagggtgc 
      tttattcgt cgagattga cgattaaag ccggatgtc 
      caatgaatc tggaggact ttccgatgg acatccaga 
      ggtgtccgc tgaaattcg gctcgttcg gatacacgg 
      ctcaggatg tcctctaca ggtggaacg ccgcgagac 
      aagtggcga ttgcagaag acatgaagc tgtcgcagt 
      tcgacctca tcgccaccc ccgctgcca atcacaccg 
      acaccatcg tcaacagcg ataccaaat cagctgaat 
      attcaatgc ttcttgtca gtttccact tgcaacgcc 
      acatgggca actttctca tccaggtgt acggccctt 
      gtgtccttc tcgtcgtcc tctcttggg tctccttct 
      ggctgaacc gcgaagcca cagctgacc gcgtctccc 
      tcggcataa ccaccgtat taacgatga catttctcg 
      gtctggaag cgagaaccg acttgccca aagttccct 
      accccacag ccctcgatt tcttcgtct tcctgtcat 
      tttccttca tctttgcaa ctattattc agttcgctg 
      tggtccact acttcacga agtacggtt ccggcgagt 
      gctacttca gctcggatt tcagttccg attccagca 
      gcgacgaag aggattgga ttgaaagca gggaaaatg 
      cgcccaaag aggtgtcgc attctccag tgtgcaaaa 
      gctcaaatt acggcagct cgaacgggg gcgatccgc 
      atttcattg aagtgattc cgctgtcag cgtgttcga 
      ttccagtgg cgtcaatga ggcgcacgt cacagcctt 
      ggagcaatc tctcctgta tcaaccgat cgcaagacg 
      ataaaaatt atcacgcga gtccgaaca aatcaactg 
      gaagccaaa aatcgccct gtcaaagcc aaaaacaca 
      gaaagaaga aaaagaaaa ggacgccga ggtttaact 
      cagtttcga aaattgatc gagcttcta gagtagtgt 
      ttcccgtcc tttttgtaa cgattaatt tgttttatt 
      ggtattcgt acctgtcga ggaccaaga gaattgtaa 
      atctgaatg tttcgtaag gaaactgtc atagctgtg 
      atgttgctt cattggtgt tagttcctc ttgcttcta 
      tttttttcg gtttggaaa gtggtagag gctatagct 
      acgtaaact gccagttga gtgtttgtt ggaaggcgt 
      tcagaacca attagagta gagtgatac aaaataaca 
      cctttgttt actaggcga aaattatat ttataacgt 
      gttaattta tggagaatc aactagttt ttagaaaaa 
      taaatcacc aacaaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY