Status
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Gene information
General information
TC008265 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7520384..7527174
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008265
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008265 RA
      MWEQVAKCV KEWSKKLGN ELYNLSTTM TRKKEIEKS 
      FSQIADKIH YESDMRLLR HITENMMKT MEKKKQAVE 
      RIAQYAEFL AYHRKNDYI EPNGTFYFY NANNITNTT 
      CDCGKEKEL EKEIQEAKC VWWCEQKDL PVTKPLKHN 
      YFTKRRIEE LFAPACDCS RPIDRTRPQ ENFNLLPLK 
      KHPNYGDTP VNLNLSVVK IATNIYERE QEVLQGVRW 
      SEPLDMIFK ENLDKDPTL KYQYFASPH GYMRHFPAV 
      KWSDERYDQ TYDPRTRSW YTEAMTSPK DVFILLDSS 
      GSVCKLKRK IAAHIVNNI LDTLNDNDF VNIYLFANS 
      TRPLVPCFK DTLVQANEE NLRLLRETL DNYKPDFQA 
      NVAVGLEKA FTLLAEFRE KGIGSLCNQ AIMLITEEA 
      FFREDEKNF FNRSNWQYG TPVRVFTYQ LERSESDAR 
      LLEWIACSN KGYFVNISL MQEVREKAL PYLNVMSRP 
      INYCHKDNP PRGELIWSY LYIDLADRR YTNWLWRKL 
      EGNRQRAIF LDHAKREFT RLQKVLTSE SHFLLQDNH 
      DYEKYEKPN EYDYMTTLS LPVYSRKAE DVDLIGVAG 
      VDIPIKLLN SLIPHHTIG VNGYAFIVT NNGYVLMHP 
      GHRREFENI PKPTFNRVD LLEVEILDD TNEPRIFDK 
      SIVKLRDKI VQQVTGSDV LKVKYALDN MKRIVLSKR 
      RYVFMSLMD SAFSVGIAL PDKYGLMVA NITIKNIAN 
      YKGGLLQTK NWAVHPDWV YCGDNSGKE PEEVVKNCL 
      LLCIQKRDL KNKNVNCDE PLFKSLLFD AEVTKWFDE 
      LIPNEAHFT KQYLVDNVF MATHSGLTR WKSLQPQTF 
      EEVVDDDEG KGKKEVFWG NKSIDEDWY KRAVQENVN 
      RNEDNFIYS VPFEISGYE YNTMITSIN AIFVTAEGK 
      KSPVAAVGV QFNHRRMLD VFNQTTAKC DSAQKPFQC 
      ACKDLSCYI LDNNAYVVL SNEKEYTGR FVGDVSPII 
      THRLIQNGV YKRIRMFDY QAICQKPPE AKSKNSATM 
      TITLFRRLL ENLLSVTDW ALATILMLI ASVAGGPDY 
      ESTKNETLL YERLEINKT VPTPCDKQM WLFTLGDSF 
      SSVDYSNKS SKAGCDWYY VIERIPHSN LLFMAANPV 
      CELDTVSHF QSHSKTPTP VEIDYNDNE TLACYIAKY 
      NNFTRRFYT KCYNYSSDE DKLAGNRTY CGHTWKP
    CDS sequence
    • >TC008265 RA
      atgtgggaa caagtagcc aaatgtgtt aaggaatgg 
      tcaaaaaaa ttaggcaat gagctttac aatctcagt 
      acgaccatg acacggaaa aaagaaatt gaaaagagt 
      tttagtcaa atcgcggac aaaatccat tatgagtca 
      gatatgcgt ctcctgcgt cacatcact gagaatatg 
      atgaaaact atggaaaag aaaaaacaa gcagttgag 
      cgtattgca caatacgct gaattttta gcttaccac 
      cgaaaaaat gattacatt gaacccaat ggaacattt 
      tatttctac aatgctaat aacataaca aacaccacg 
      tgtgactgt ggtaaggaa aaagagttg gagaaggag 
      atacaagaa gctaagtgt gtttggtgg tgcgaacag 
      aaagatcta ccggtgact aaacccttg aagcacaat 
      tatttcaca aagaggcgg attgaagaa ttgtttgcc 
      cctgcttgt gactgttct agacccatt gacaggacc 
      cgaccgcag gaaaatttt aatttgcta ccactgaag 
      aaacacccg aattatgga gacactccg gttaattta 
      aacttaagc gttgttaaa attgccaca aatatttac 
      gagcgagaa caagaagta ctacaaggg gtgcgttgg 
      agcgaacct ttagatatg atttttaaa gagaatttg 
      gataaagat ccgacgctc aaataccag tactttgcc 
      agtccgcat ggttatatg cgacatttt cctgctgtg 
      aagtggtct gatgagcgg tacgaccag acgtacgac 
      cctcgaacc cgctcctgg tacaccgaa gcaatgact 
      tcgccaaaa gatgtgttc attcttttg gacagttcg 
      ggatctgtc tgtaaactc aaaagaaaa attgcggct 
      catatagtt aacaatatt ttggatact ctcaacgat 
      aacgacttt gtgaacatt tatctcttt gcaaatagt 
      actaggccg ctagtaccg tgtttcaag gacactcta 
      gtgcaagca aacgaggag aatctccgc ttgttgcgg 
      gagactttg gacaattac aagcccgac ttccaggca 
      aacgtggca gttggactg gagaaagcc ttcactctt 
      ttggccgaa tttcgggag aaagggatc ggttcgctg 
      tgtaaccag gcaataatg ctgataaca gaagaggca 
      tttttccgc gaagacgag aagaacttt ttcaaccgt 
      tcaaactgg cagtacggc actccggtc cgtgttttc 
      acgtatcag ctcgaacgg agcgaatcc gacgcgcgg 
      cttttggag tggattgcg tgttcgaat aagggatat 
      ttcgtgaat atttcccta atgcaagaa gttcgggag 
      aaagcattg ccgtatttg aatgtaatg tcacgacct 
      attaattac tgtcacaaa gacaaccct ccgagaggt 
      gaactgata tggagttat ttatacatc gatctcgcg 
      gatcgcaga tacacaaat tggctgtgg aggaagctg 
      gagggtaac aggcaacga gcgattttt ctggaccat 
      gcgaagcgg gagtttaca agattgcag aaagttcta 
      acaagcgag tcgcatttt ctgctccaa gacaatcat 
      gattatgaa aagtacgag aaaccgaat gagtatgat 
      tacatgacg acactttcc ctccccgtt tacagtcgg 
      aaagctgaa gatgtcgat cttattgga gtcgctgga 
      gttgacatc cctatcaag cttttaaac tcgttaatt 
      ccccaccac acgattgga gtgaatggg tacgcattt 
      attgtcaca aataacggc tatgttctc atgcatcca 
      ggacacagg cgcgagttt gaaaatatc cccaaaccg 
      acgttcaac agagtggat ttactcgaa gtggaaatt 
      ttagacgac acaaacgaa ccgcgaatt ttcgataaa 
      tcaattgtg aagctgagg gacaaaata gtgcaacaa 
      gtcaccggt tccgatgta ttgaaagtc aaatatgca 
      ttggacaat atgaaacgc atcgtgttg agcaagcga 
      aggtacgtt ttcatgtct ttgatggac tccgctttc 
      agcgttggc atcgcctta cccgacaaa tacggtctc 
      atggtggcc aacattacg attaaaaat atcgcgaat 
      tataaaggc ggcctactc cagacaaaa aattgggcc 
      gtacatcca gactgggtt tactgcggc gacaacagc 
      ggaaaagaa ccggaagaa gtcgtgaaa aattgttta 
      ctactttgc attcaaaag cgggacttg aaaaacaaa 
      aatgtaaac tgtgacgaa ccacttttt aagtcgctc 
      ttatttgat gcggaggtc actaaatgg tttgacgaa 
      ctgattcca aacgaagcc cattttacg aaacaatat 
      ttagttgat aatgttttc atggccacc cactcgggc 
      ctgactcgt tggaagtcg ctccagcca caaactttc 
      gaagaggtg gtcgatgat gacgaaggt aaaggcaag 
      aaagaggtt ttttggggg aacaaatca atcgatgaa 
      gactggtac aaaagggcc gttcaagag aatgtcaac 
      agaaacgaa gacaatttc atttattcc gtcccgttt 
      gaaatctca ggatacgaa tacaacacg atgattaca 
      agcattaat gcgatcttt gtaacagct gaagggaaa 
      aagtcaccg gtggcggcc gtcggggtc cagtttaac 
      caccgccga atgcttgac gttttcaac caaaccacc 
      gcaaaatgt gacagtgcc caaaagccg ttccaatgc 
      gcctgcaaa gatttgtct tgctatatc ctggacaat 
      aacgcttat gtcgtgcta agtaacgag aaggaatat 
      acagggcga tttgttggg gatgttagc cccattata 
      acccaccgt ttgatacaa aacggcgtc tacaaaaga 
      atcagaatg tttgactac caggcaata tgtcagaaa 
      ccaccagag gccaaaagc aaaaattct gccaccatg 
      actattact ttattcaga agattgctt gaaaattta 
      ttaagtgtg accgattgg gctctagct acgatttta 
      atgttgatt gcttcggtg gccggaggc ccggattat 
      gaaagtaca aagaatgag acacttctg tacgaaaga 
      ctcgaaatt aacaaaacc gtgccaaca ccttgcgac 
      aaacagatg tggttgttt acgttgggt gatagtttt 
      agttccgtt gattattcg aacaagtcg tcaaaagcc 
      ggatgtgat tggtattat gtcatcgaa cgcatcccc 
      cacagtaat ctgttgttt atggccgca aatccagtt 
      tgcgaactg gatacagtg agccatttc cagtcccat 
      tctaaaacg cctacacct gttgaaatt gactacaat 
      gacaacgag actcttgct tgctacatc gccaaatac 
      aacaatttc actcggagg ttttatacg aaatgttac 
      aattacagc tccgatgag gataagctc gccgggaac 
      aggacgtat tgtggccac acttggaag ccataa
    mRNA sequence
    • >TC008265 RA
      taataaaag cgagcaaag tatgtggga acaagtagc 
      caaatgtgt taaggaatg gtcaaaaaa attaggcaa 
      tgagcttta caatctcag tacgaccat gacacggaa 
      aaaagaaat tgaaaagag ttttagtca aatcgcgga 
      caaaatcca ttatgagtc agatatgcg tctcctgcg 
      tcacatcac tgagaatat gatgaaaac tatggaaaa 
      gaaaaaaca agcagttga gcgtattgc acaatacgc 
      tgaattttt agcttacca ccgaaaaaa tgattacat 
      tgaacccaa tggaacatt ttatttcta caatgctaa 
      taacataac aaacaccac gtgtgactg tggtaagga 
      aaaagagtt ggagaagga gatacaaga agctaagtg 
      tgtttggtg gtgcgaaca gaaagatct accggtgac 
      taaaccctt gaagcacaa ttatttcac aaagaggcg 
      gattgaaga attgtttgc ccctgcttg tgactgttc 
      tagacccat tgacaggac ccgaccgca ggaaaattt 
      taatttgct accactgaa gaaacaccc gaattatgg 
      agacactcc ggttaattt aaacttaag cgttgttaa 
      aattgccac aaatattta cgagcgaga acaagaagt 
      actacaagg ggtgcgttg gagcgaacc tttagatat 
      gatttttaa agagaattt ggataaaga tccgacgct 
      caaatacca gtactttgc cagtccgca tggttatat 
      gcgacattt tcctgctgt gaagtggtc tgatgagcg 
      gtacgacca gacgtacga ccctcgaac ccgctcctg 
      gtacaccga agcaatgac ttcgccaaa agatgtgtt 
      cattctttt ggacagttc gggatctgt ctgtaaact 
      caaaagaaa aattgcggc tcatatagt taacaatat 
      tttggatac tctcaacga taacgactt tgtgaacat 
      ttatctctt tgcaaatag tactaggcc gctagtacc 
      gtgtttcaa ggacactct agtgcaagc aaacgagga 
      gaatctccg cttgttgcg ggagacttt ggacaatta 
      caagcccga cttccaggc aaacgtggc agttggact 
      ggagaaagc cttcactct tttggccga atttcggga 
      gaaagggat cggttcgct gtgtaacca ggcaataat 
      gctgataac agaagaggc atttttccg cgaagacga 
      gaagaactt tttcaaccg ttcaaactg gcagtacgg 
      cactccggt ccgtgtttt cacgtatca gctcgaacg 
      gagcgaatc cgacgcgcg gcttttgga gtggattgc 
      gtgttcgaa taagggata tttcgtgaa tatttccct 
      aatgcaaga agttcggga gaaagcatt gccgtattt 
      gaatgtaat gtcacgacc tattaatta ctgtcacaa 
      agacaaccc tccgagagg tgaactgat atggagtta 
      tttatacat cgatctcgc ggatcgcag atacacaaa 
      ttggctgtg gaggaagct ggagggtaa caggcaacg 
      agcgatttt tctggacca tgcgaagcg ggagtttac 
      aagattgca gaaagttct aacaagcga gtcgcattt 
      tctgctcca agacaatca tgattatga aaagtacga 
      gaaaccgaa tgagtatga ttacatgac gacactttc 
      cctccccgt ttacagtcg gaaagctga agatgtcga 
      tcttattgg agtcgctgg agttgacat ccctatcaa 
      gcttttaaa ctcgttaat tccccacca cacgattgg 
      agtgaatgg gtacgcatt tattgtcac aaataacgg 
      ctatgttct catgcatcc aggacacag gcgcgagtt 
      tgaaaatat ccccaaacc gacgttcaa cagagtgga 
      tttactcga agtggaaat tttagacga cacaaacga 
      accgcgaat tttcgataa atcaattgt gaagctgag 
      ggacaaaat agtgcaaca agtcaccgg ttccgatgt 
      attgaaagt caaatatgc attggacaa tatgaaacg 
      catcgtgtt gagcaagcg aaggtacgt tttcatgtc 
      tttgatgga ctccgcttt cagcgttgg catcgcctt 
      acccgacaa atacggtct catggtggc caacattac 
      gattaaaaa tatcgcgaa ttataaagg cggcctact 
      ccagacaaa aaattgggc cgtacatcc agactgggt 
      ttactgcgg cgacaacag cggaaaaga accggaaga 
      agtcgtgaa aaattgttt actactttg cattcaaaa 
      gcgggactt gaaaaacaa aaatgtaaa ctgtgacga 
      accactttt taagtcgct cttatttga tgcggaggt 
      cactaaatg gtttgacga actgattcc aaacgaagc 
      ccattttac gaaacaata tttagttga taatgtttt 
      catggccac ccactcggg cctgactcg ttggaagtc 
      gctccagcc acaaacttt cgaagaggt ggtcgatga 
      tgacgaagg taaaggcaa gaaagaggt tttttgggg 
      gaacaaatc aatcgatga agactggta caaaagggc 
      cgttcaaga gaatgtcaa cagaaacga agacaattt 
      catttattc cgtcccgtt tgaaatctc aggatacga 
      atacaacac gatgattac aagcattaa tgcgatctt 
      tgtaacagc tgaagggaa aaagtcacc ggtggcggc 
      cgtcggggt ccagtttaa ccaccgccg aatgcttga 
      cgttttcaa ccaaaccac cgcaaaatg tgacagtgc 
      ccaaaagcc gttccaatg cgcctgcaa agatttgtc 
      ttgctatat cctggacaa taacgctta tgtcgtgct 
      aagtaacga gaaggaata tacagggcg atttgttgg 
      ggatgttag ccccattat aacccaccg tttgataca 
      aaacggcgt ctacaaaag aatcagaat gtttgacta 
      ccaggcaat atgtcagaa accaccaga ggccaaaag 
      caaaaattc tgccaccat gactattac tttattcag 
      aagattgct tgaaaattt attaagtgt gaccgattg 
      ggctctagc tacgatttt aatgttgat tgcttcggt 
      ggccggagg cccggatta tgaaagtac aaagaatga 
      gacacttct gtacgaaag actcgaaat taacaaaac 
      cgtgccaac accttgcga caaacagat gtggttgtt 
      tacgttggg tgatagttt tagttccgt tgattattc 
      gaacaagtc gtcaaaagc cggatgtga ttggtatta 
      tgtcatcga acgcatccc ccacagtaa tctgttgtt 
      tatggccgc aaatccagt ttgcgaact ggatacagt 
      gagccattt ccagtccca ttctaaaac gcctacacc 
      tgttgaaat tgactacaa tgacaacga gactcttgc 
      ttgctacat cgccaaata caacaattt cactcggag 
      gttttatac gaaatgtta caattacag ctccgatga 
      ggataagct cgccgggaa caggacgta ttgtggcca 
      cacttggaa gccataatt tgtgtctgt attgcttga 
      aataaattt gcattggaa at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY