| General information | |||
| TC008236 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 7104735..7108053 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008236 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07210 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC008236 RA
MRYFVVLCC VLASVMAKE KKISLEDIE RDNLKTTGK IARAPPKIP VASPTDYGF VPTKTVTDY ARQQPKYVQ YVPQTYAQP AQQYTPPPQ QYATVPQQY YYQQQQSVQ NPYQQYENV QYVTDNSIA QAQQQYYTP QYVYLQQYT APSTAIQSV VDPKGGIQY VMYVVPKAE QTQNYENVV YTNSNEQQQ YVVPQTQYV QTQQVTPEI KYAPQPEKP QQLFTKSEP KSLLDSYVP SILQVQYYK QQQAQANAI QHSLKDVST VSGKSSYRP TQISHNSAE SVINYHYQL PSIQQQHQ
- >TC008236 RB
MAKEKKISL EDIERDNLK TTGKIARAP PKIPVASPT DYGFVPTKT VTDYARQQP KYVQYVPQT YAQPAQQYT PPPQQYATV PQQYYYQQQ QSVQNPYQQ YENVQYVTD NSIAQAQQQ YYTPQYVYL QQYTAPSTA IQSVVDPKG GIQYVMYVV PKAEQTQNY ENVVYTNSN EQQQYVVPQ TQYVQTQQV TPEIKYAPQ PEKPQQLFT KSEPKSLLD SYVPSILQV QYYKQQQAQ ANAIQHSLK DVSTVSGKS SYRPTQISH NSAESVINY HYQLPSIQQ QHQ
- >TC008236 RA
atgagatac ttcgtggta ctttgttgt gtgttggcg tcggtcatg gccaaggaa aaaaagatc agtttagaa gacatcgaa agggacaat ttgaaaacc accgggaaa attgcgcga gcacccccg aaaatcccc gttgcttcc ccaactgac tacggtttc gtgcccaca aaaacagtg actgactat gcaagacaa cagcccaag tatgtccaa tacgtccct caaacgtac gcccagccc gcccaacag tacacaccc cctccccaa cagtacgcc acagtaccc cagcagtac tactaccag caacagcaa tccgtgcaa aacccttac cagcaatac gaaaacgtg caatacgtc acggacaac agcattgcc caagcacaa cagcagtac tacaccccc caatacgtg tacctccaa cagtacact gcccccagt actgccatc cagagcgtg gttgacccc aaagggggg atccagtac gtcatgtat gtggtaccc aaagccgaa caaacccaa aattacgaa aatgtggtt tataccaac tctaacgaa caacaacag tatgtggta ccacaaacc cagtacgtc caaacccag caagtgacg cctgaaatc aaatacgcg cctcagccc gagaaacca caacaattg ttcacaaag agtgaacct aagagtctg ttggattcg tacgtgccg tcgatttta caagtccag tattacaag caacaacaa gcgcaagct aatgctatt caacactcg ttgaaagat gtgagtact gtctccggc aagagtagc tacagaccg acgcaaatc agtcacaat agtgcggag agtgtcatc aattaccac taccagctc ccgagtatc caacaacag caccaataa
- >TC008236 RB
atggccaag gaaaaaaag atcagttta gaagacatc gaaagggac aatttgaaa accaccggg aaaattgcg cgagcaccc ccgaaaatc cccgttgct tccccaact gactacggt ttcgtgccc acaaaaaca gtgactgac tatgcaaga caacagccc aagtatgtc caatacgtc cctcaaacg tacgcccag cccgcccaa cagtacaca ccccctccc caacagtac gccacagta ccccagcag tactactac cagcaacag caatccgtg caaaaccct taccagcaa tacgaaaac gtgcaatac gtcacggac aacagcatt gcccaagca caacagcag tactacacc ccccaatac gtgtacctc caacagtac actgccccc agtactgcc atccagagc gtggttgac cccaaaggg gggatccag tacgtcatg tatgtggta cccaaagcc gaacaaacc caaaattac gaaaatgtg gtttatacc aactctaac gaacaacaa cagtatgtg gtaccacaa acccagtac gtccaaacc cagcaagtg acgcctgaa atcaaatac gcgcctcag cccgagaaa ccacaacaa ttgttcaca aagagtgaa cctaagagt ctgttggat tcgtacgtg ccgtcgatt ttacaagtc cagtattac aagcaacaa caagcgcaa gctaatgct attcaacac tcgttgaaa gatgtgagt actgtctcc ggcaagagt agctacaga ccgacgcaa atcagtcac aatagtgcg gagagtgtc atcaattac cactaccag ctcccgagt atccaacaa cagcaccaa taa
- >TC008236 RA
ctcacttaa gaaagtaca aatggaagt tctttggta gaggatcat tattttgtg ccgatcctt ccaccgaac tgtatcatt tcactttcg cagacatga gatacttcg tggtacttt gttgtgtgt tggcgtcgg tcatggcca aggaaaaaa agatcagtt tagaagaca tcgaaaggg acaatttga aaaccaccg ggaaaattg cgcgagcac ccccgaaaa tccccgttg cttccccaa ctgactacg gtttcgtgc ccacaaaaa cagtgactg actatgcaa gacaacagc ccaagtatg tccaatacg tccctcaaa cgtacgccc agcccgccc aacagtaca caccccctc cccaacagt acgccacag taccccagc agtactact accagcaac agcaatccg tgcaaaacc cttaccagc aatacgaaa acgtgcaat acgtcacgg acaacagca ttgcccaag cacaacagc agtactaca ccccccaat acgtgtacc tccaacagt acactgccc ccagtactg ccatccaga gcgtggttg accccaaag gggggatcc agtacgtca tgtatgtgg tacccaaag ccgaacaaa cccaaaatt acgaaaatg tggtttata ccaactcta acgaacaac aacagtatg tggtaccac aaacccagt acgtccaaa cccagcaag tgacgcctg aaatcaaat acgcgcctc agcccgaga aaccacaac aattgttca caaagagtg aacctaaga gtctgttgg attcgtacg tgccgtcga ttttacaag tccagtatt acaagcaac aacaagcgc aagctaatg ctattcaac actcgttga aagatgtga gtactgtct ccggcaaga gtagctaca gaccgacgc aaatcagtc acaatagtg cggagagtg tcatcaatt accactacc agctcccga gtatccaac aacagcacc aataatttt ttgttacca ttttattaa atcgtgatt ttagttgta agtcatatt ctttcttat ttatgtcgt ttcttaacg tcttgtctt ttcactttt ctactttta tagcaactg ttagaactt tatcggcca gtttcttct tttcaccac tatttattt tccattctg tttcttcac atgttattc gtttttatg tacaattta tgtattatg tacgtttat aaaacaaat aaaatactt ttaaaaat
- >TC008236 RB
ttggtagag gatcattat tttgtgccg atccttcca ccgaactgt atcatttca ctttcgcag acatgagat acttcgtgg tactttgtt gtgtgttgg cgtcggtca tggccaagg aaaaaaaga tcagtttag aagacatcg aaagggaca atttgaaaa ccaccggga aaattgcgc gagcacccc cgaaaatcc ccgttgctt ccccaactg actacggtt tcgtgccca caaaaacag tgactgact atgcaagac aacagccca agtatgtcc aatacgtcc ctcaaacgt acgcccagc ccgcccaac agtacacac cccctcccc aacagtacg ccacagtac cccagcagt actactacc agcaacagc aatccgtgc aaaaccctt accagcaat acgaaaacg tgcaatacg tcacggaca acagcattg cccaagcac aacagcagt actacaccc cccaatacg tgtacctcc aacagtaca ctgccccca gtactgcca tccagagcg tggttgacc ccaaagggg ggatccagt acgtcatgt atgtggtac ccaaagccg aacaaaccc aaaattacg aaaatgtgg tttatacca actctaacg aacaacaac agtatgtgg taccacaaa cccagtacg tccaaaccc agcaagtga cgcctgaaa tcaaatacg cgcctcagc ccgagaaac cacaacaat tgttcacaa agagtgaac ctaagagtc tgttggatt cgtacgtgc cgtcgattt tacaagtcc agtattaca agcaacaac aagcgcaag ctaatgcta ttcaacact cgttgaaag atgtgagta ctgtctccg gcaagagta gctacagac cgacgcaaa tcagtcaca atagtgcgg agagtgtca tcaattacc actaccagc tcccgagta tccaacaac agcaccaat aattttttg ttaccattt tattaaatc gtgatttta gttgtaagt catattctt tcttattta tgtcgtttc ttaacgtct tgtcttttc acttttcta cttttatag caactgtta gaactttat cggccagtt tcttctttt caccactat ttattttcc attctgttt cttcacatg ttattcgtt tttatgtac aatttatgt attatgtac gtttataaa acaaataaa atactttta aaaat
