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Gene information
General information
TC008236 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7104735..7108053
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC008236
      iBeetle screen iB_07210

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008236 RA
        MRYFVVLCC VLASVMAKE KKISLEDIE RDNLKTTGK 
IARAPPKIP VASPTDYGF VPTKTVTDY ARQQPKYVQ 
YVPQTYAQP AQQYTPPPQ QYATVPQQY YYQQQQSVQ 
NPYQQYENV QYVTDNSIA QAQQQYYTP QYVYLQQYT 
APSTAIQSV VDPKGGIQY VMYVVPKAE QTQNYENVV 
YTNSNEQQQ YVVPQTQYV QTQQVTPEI KYAPQPEKP 
QQLFTKSEP KSLLDSYVP SILQVQYYK QQQAQANAI 
QHSLKDVST VSGKSSYRP TQISHNSAE SVINYHYQL 
PSIQQQHQ
      • >TC008236 RB
        MAKEKKISL EDIERDNLK TTGKIARAP PKIPVASPT 
DYGFVPTKT VTDYARQQP KYVQYVPQT YAQPAQQYT 
PPPQQYATV PQQYYYQQQ QSVQNPYQQ YENVQYVTD 
NSIAQAQQQ YYTPQYVYL QQYTAPSTA IQSVVDPKG 
GIQYVMYVV PKAEQTQNY ENVVYTNSN EQQQYVVPQ 
TQYVQTQQV TPEIKYAPQ PEKPQQLFT KSEPKSLLD 
SYVPSILQV QYYKQQQAQ ANAIQHSLK DVSTVSGKS 
SYRPTQISH NSAESVINY HYQLPSIQQ QHQ
      CDS sequence
      • >TC008236 RA
        atgagatac ttcgtggta ctttgttgt gtgttggcg 
tcggtcatg gccaaggaa aaaaagatc agtttagaa 
gacatcgaa agggacaat ttgaaaacc accgggaaa 
attgcgcga gcacccccg aaaatcccc gttgcttcc 
ccaactgac tacggtttc gtgcccaca aaaacagtg 
actgactat gcaagacaa cagcccaag tatgtccaa 
tacgtccct caaacgtac gcccagccc gcccaacag 
tacacaccc cctccccaa cagtacgcc acagtaccc 
cagcagtac tactaccag caacagcaa tccgtgcaa 
aacccttac cagcaatac gaaaacgtg caatacgtc 
acggacaac agcattgcc caagcacaa cagcagtac 
tacaccccc caatacgtg tacctccaa cagtacact 
gcccccagt actgccatc cagagcgtg gttgacccc 
aaagggggg atccagtac gtcatgtat gtggtaccc 
aaagccgaa caaacccaa aattacgaa aatgtggtt 
tataccaac tctaacgaa caacaacag tatgtggta 
ccacaaacc cagtacgtc caaacccag caagtgacg 
cctgaaatc aaatacgcg cctcagccc gagaaacca 
caacaattg ttcacaaag agtgaacct aagagtctg 
ttggattcg tacgtgccg tcgatttta caagtccag 
tattacaag caacaacaa gcgcaagct aatgctatt 
caacactcg ttgaaagat gtgagtact gtctccggc 
aagagtagc tacagaccg acgcaaatc agtcacaat 
agtgcggag agtgtcatc aattaccac taccagctc 
ccgagtatc caacaacag caccaataa
      • >TC008236 RB
        atggccaag gaaaaaaag atcagttta gaagacatc 
gaaagggac aatttgaaa accaccggg aaaattgcg 
cgagcaccc ccgaaaatc cccgttgct tccccaact 
gactacggt ttcgtgccc acaaaaaca gtgactgac 
tatgcaaga caacagccc aagtatgtc caatacgtc 
cctcaaacg tacgcccag cccgcccaa cagtacaca 
ccccctccc caacagtac gccacagta ccccagcag 
tactactac cagcaacag caatccgtg caaaaccct 
taccagcaa tacgaaaac gtgcaatac gtcacggac 
aacagcatt gcccaagca caacagcag tactacacc 
ccccaatac gtgtacctc caacagtac actgccccc 
agtactgcc atccagagc gtggttgac cccaaaggg 
gggatccag tacgtcatg tatgtggta cccaaagcc 
gaacaaacc caaaattac gaaaatgtg gtttatacc 
aactctaac gaacaacaa cagtatgtg gtaccacaa 
acccagtac gtccaaacc cagcaagtg acgcctgaa 
atcaaatac gcgcctcag cccgagaaa ccacaacaa 
ttgttcaca aagagtgaa cctaagagt ctgttggat 
tcgtacgtg ccgtcgatt ttacaagtc cagtattac 
aagcaacaa caagcgcaa gctaatgct attcaacac 
tcgttgaaa gatgtgagt actgtctcc ggcaagagt 
agctacaga ccgacgcaa atcagtcac aatagtgcg 
gagagtgtc atcaattac cactaccag ctcccgagt 
atccaacaa cagcaccaa taa
      mRNA sequence
      • >TC008236 RA
        ctcacttaa gaaagtaca aatggaagt tctttggta 
gaggatcat tattttgtg ccgatcctt ccaccgaac 
tgtatcatt tcactttcg cagacatga gatacttcg 
tggtacttt gttgtgtgt tggcgtcgg tcatggcca 
aggaaaaaa agatcagtt tagaagaca tcgaaaggg 
acaatttga aaaccaccg ggaaaattg cgcgagcac 
ccccgaaaa tccccgttg cttccccaa ctgactacg 
gtttcgtgc ccacaaaaa cagtgactg actatgcaa 
gacaacagc ccaagtatg tccaatacg tccctcaaa 
cgtacgccc agcccgccc aacagtaca caccccctc 
cccaacagt acgccacag taccccagc agtactact 
accagcaac agcaatccg tgcaaaacc cttaccagc 
aatacgaaa acgtgcaat acgtcacgg acaacagca 
ttgcccaag cacaacagc agtactaca ccccccaat 
acgtgtacc tccaacagt acactgccc ccagtactg 
ccatccaga gcgtggttg accccaaag gggggatcc 
agtacgtca tgtatgtgg tacccaaag ccgaacaaa 
cccaaaatt acgaaaatg tggtttata ccaactcta 
acgaacaac aacagtatg tggtaccac aaacccagt 
acgtccaaa cccagcaag tgacgcctg aaatcaaat 
acgcgcctc agcccgaga aaccacaac aattgttca 
caaagagtg aacctaaga gtctgttgg attcgtacg 
tgccgtcga ttttacaag tccagtatt acaagcaac 
aacaagcgc aagctaatg ctattcaac actcgttga 
aagatgtga gtactgtct ccggcaaga gtagctaca 
gaccgacgc aaatcagtc acaatagtg cggagagtg 
tcatcaatt accactacc agctcccga gtatccaac 
aacagcacc aataatttt ttgttacca ttttattaa 
atcgtgatt ttagttgta agtcatatt ctttcttat 
ttatgtcgt ttcttaacg tcttgtctt ttcactttt 
ctactttta tagcaactg ttagaactt tatcggcca 
gtttcttct tttcaccac tatttattt tccattctg 
tttcttcac atgttattc gtttttatg tacaattta 
tgtattatg tacgtttat aaaacaaat aaaatactt 
ttaaaaat
      • >TC008236 RB
        ttggtagag gatcattat tttgtgccg atccttcca 
ccgaactgt atcatttca ctttcgcag acatgagat 
acttcgtgg tactttgtt gtgtgttgg cgtcggtca 
tggccaagg aaaaaaaga tcagtttag aagacatcg 
aaagggaca atttgaaaa ccaccggga aaattgcgc 
gagcacccc cgaaaatcc ccgttgctt ccccaactg 
actacggtt tcgtgccca caaaaacag tgactgact 
atgcaagac aacagccca agtatgtcc aatacgtcc 
ctcaaacgt acgcccagc ccgcccaac agtacacac 
cccctcccc aacagtacg ccacagtac cccagcagt 
actactacc agcaacagc aatccgtgc aaaaccctt 
accagcaat acgaaaacg tgcaatacg tcacggaca 
acagcattg cccaagcac aacagcagt actacaccc 
cccaatacg tgtacctcc aacagtaca ctgccccca 
gtactgcca tccagagcg tggttgacc ccaaagggg 
ggatccagt acgtcatgt atgtggtac ccaaagccg 
aacaaaccc aaaattacg aaaatgtgg tttatacca 
actctaacg aacaacaac agtatgtgg taccacaaa 
cccagtacg tccaaaccc agcaagtga cgcctgaaa 
tcaaatacg cgcctcagc ccgagaaac cacaacaat 
tgttcacaa agagtgaac ctaagagtc tgttggatt 
cgtacgtgc cgtcgattt tacaagtcc agtattaca 
agcaacaac aagcgcaag ctaatgcta ttcaacact 
cgttgaaag atgtgagta ctgtctccg gcaagagta 
gctacagac cgacgcaaa tcagtcaca atagtgcgg 
agagtgtca tcaattacc actaccagc tcccgagta 
tccaacaac agcaccaat aattttttg ttaccattt 
tattaaatc gtgatttta gttgtaagt catattctt 
tcttattta tgtcgtttc ttaacgtct tgtcttttc 
acttttcta cttttatag caactgtta gaactttat 
cggccagtt tcttctttt caccactat ttattttcc 
attctgttt cttcacatg ttattcgtt tttatgtac 
aatttatgt attatgtac gtttataaa acaaataaa 
atactttta aaaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY