Status
Please wait ...
Gene information
General information
TC011460 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6495427..6503231
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011460
    iBeetle screen iB_07208

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva segment potentially number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    maxilla number increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    labium number increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: potentially transformed into maxilla and labium
    abdomen segment number decreased -
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011460 RA
      MPLLQETTP KRDMLDSQE KTPLSSVSY PSMFTPSQL 
      LMASHLMAA SRLSLPTNP AFFHPGLLP LAWQANSPP 
      SPPAPSELP ALKSRKLNN NNVVSSTNQ EIRGPKRKT 
      WKVEEDSPS PTSSVSPEV KDSSRDRPF TCEVCNRSF 
      GYKHVLQNH ERTHTGEKP FECQECHKR FTRDHHLKT 
      HMRLHTGER PYRCEHCDR QFVQVANLR RHLRVHTGE 
      RPYGCEHCS MKFSDSNQL KAHVLIHTN EKPFECDKC 
      RGRFRRRHH LVHHKCGGE EEAERARHP PSVPLVTLP 
      LRVALPEQT EPEDLSMTT GLHSHSSGE SPLSRSPSS 
      REEDDEDLD PHEAAAIFL HT
    CDS sequence
    • >TC011460 RA
      atgccgctg ctgcaagaa accactccc aaaagagac 
      atgctggac tctcaggag aagaccccg ttatcgtcg 
      gtctcgtac ccatcgatg ttcacccca tctcaactc 
      ctgatggcc agccacctc atggccgcc tcccgccta 
      agcctcccc accaacccc gctttcttc caccccggc 
      ctcctcccg ctcgcctgg caagccaat tccccccca 
      tctcccccg gcccccagc gagctcccg gccctaaaa 
      tcgcgcaaa ctgaacaac aacaacgtc gtcagcagc 
      accaaccag gagatccgc ggcccgaaa cgaaaaacg 
      tggaaggtg gaggaggac agtccttcg cccacgtcg 
      agcgtctcc cccgaggtg aaggactcc agccgggac 
      cggcccttc acctgcgag gtgtgcaac cgctcgttc 
      ggctacaag cacgtcctg cagaaccac gagcgcacc 
      cacacgggg gagaagccc ttcgagtgc caggagtgc 
      cacaagcgg ttcacgcgc gaccaccac ctcaagacc 
      cacatgcgg ctgcacacg ggcgagcgg ccctaccgc 
      tgcgagcac tgcgaccgg cagttcgtc caggtggcc 
      aacctgcgg cggcacctg cgggtgcac accggggag 
      cggccctac ggctgcgag cactgctcg atgaagttc 
      tccgactcg aaccagctc aaggcgcac gtgctcatc 
      cacacgaac gagaagccc ttcgagtgc gacaagtgc 
      cgcggcagg ttccgccgg cggcaccac ctggtgcac 
      cacaagtgc ggcggcgag gaggaggcc gagcgggcg 
      cggcacccg ccgtccgtg ccgctggtg acgctgccg 
      ctgcgcgtg gcgctgccc gagcagacg gagccggag 
      gacctctcc atgaccacc ggcctgcac tcgcacagc 
      agcggcgag tcgccgctg tccaggtcc cccagctcg 
      cgggaggag gacgacgag gacctggac ccgcacgaa 
      gccgccgcc atcttcctg cacacctag
    mRNA sequence
    • >TC011460 RA
      tcgtgcgtg cagaatttt tacattcag taagcgact 
      gtttggctt gtgctacgg ttggttctc tgaaactct 
      ccaccacac acgatcgca acacacatg agaaacaaa 
      agcaaagag actgtgaca gtggcgcac gtgatggac 
      attttcgag tgagaaaaa cgacggaaa atgccgctg 
      ctgcaagaa accactccc aaaagagac atgctggac 
      tctcaggag aagaccccg ttatcgtcg gtctcgtac 
      ccatcgatg ttcacccca tctcaactc ctgatggcc 
      agccacctc atggccgcc tcccgccta agcctcccc 
      accaacccc gctttcttc caccccggc ctcctcccg 
      ctcgcctgg caagccaat tccccccca tctcccccg 
      gcccccagc gagctcccg gccctaaaa tcgcgcaaa 
      ctgaacaac aacaacgtc gtcagcagc accaaccag 
      gagatccgc ggcccgaaa cgaaaaacg tggaaggtg 
      gaggaggac agtccttcg cccacgtcg agcgtctcc 
      cccgaggtg aaggactcc agccgggac cggcccttc 
      acctgcgag gtgtgcaac cgctcgttc ggctacaag 
      cacgtcctg cagaaccac gagcgcacc cacacgggg 
      gagaagccc ttcgagtgc caggagtgc cacaagcgg 
      ttcacgcgc gaccaccac ctcaagacc cacatgcgg 
      ctgcacacg ggcgagcgg ccctaccgc tgcgagcac 
      tgcgaccgg cagttcgtc caggtggcc aacctgcgg 
      cggcacctg cgggtgcac accggggag cggccctac 
      ggctgcgag cactgctcg atgaagttc tccgactcg 
      aaccagctc aaggcgcac gtgctcatc cacacgaac 
      gagaagccc ttcgagtgc gacaagtgc cgcggcagg 
      ttccgccgg cggcaccac ctggtgcac cacaagtgc 
      ggcggcgag gaggaggcc gagcgggcg cggcacccg 
      ccgtccgtg ccgctggtg acgctgccg ctgcgcgtg 
      gcgctgccc gagcagacg gagccggag gacctctcc 
      atgaccacc ggcctgcac tcgcacagc agcggcgag 
      tcgccgctg tccaggtcc cccagctcg cgggaggag 
      gacgacgag gacctggac ccgcacgaa gccgccgcc 
      atcttcctg cacacctag tcgcaggta cttgttttg 
      tacagttga ttggtatgc gagttcctc cagagatga 
      tgcaagccg gtgactatt gttagttga gtttgttag 
      gtacctgcc tttgctaat ttgcatggg acgaaggcc 
      gatgctcta gttaaagtt ttgtggaat tttgcggac 
      gacgagatg agggaccaa agtgtttcg agttttaaa 
      ttaacttgc ttttgtcgg atttttctt ttttttttc 
      aatatcctg ttctcattc atgtgttag tcgtcattc 
      gtcaagact tgtttacaa cgcacgatt tttacgaaa 
      ttcgatacc ttcgaccct ttggggtga caagcgcgc 
      gatctgact ttatcggtt tcaaggtgg aaagtggaa 
      acgaatcga aaagacggt tttaaacta caatcagac 
      agatgttca agaacttgg acaaaatta tagcaaaag 
      gacggctat tttaactaa tttttttct catggtaaa 
      gccaaaaag ctacgactt ggcggttaa tgccaacat 
      gttttaaat aattaaagc aaaaattca aataaacac 
      agatttgac gaaaaaaaa ttaataacc aaaagtttg 
      aagaaaaag aggctaaga tataagaaa aaaatgtct 
      attgctgta agaattttc taaagttag ttcaatttc 
      attcaaaca cgactacat tatgtattt aatttatac 
      attttaatt atttattat ttgaagaaa caataaaag 
      aaggaatta aaataaagt taaaaaaga aataataaa 
      acattaaac aataaaaaa ataataata tctgaagct 
      ctttgactt gataattcc caaaaacta aaacgaatt 
      gagaagttg tttgccgac ttaaaattc cgattaaaa 
      attagataa aaattgaca aaaaaaggt ttgacaaaa 
      aaaattggg aaacttttg aaaaaaacg aaaactcaa 
      aactattac tagccacgt atttgaaga aaaacagaa 
      gaaaaatat tagagagaa aaaaaaaag aaattaaac 
      ataatgtat tggtgtaag aagtttctg aagttagtt 
      ttttttttc aatctaaca cgactactt tttccaatt 
      tgttctgaa ttaaattaa ttcatttta aatacttat 
      ttaatgact ggaatctga tagacaaga aaaaaaaca 
      aaagaaaaa gttaattta aaaaataag ttaggaaaa 
      caaagttaa aaaagaaac aaacaaaca ataaaaaaa 
      taactaaaa ttgatgctt tttgatttg atgattttc 
      aaaaattaa aacgtaccg agaagttgt gtgcctact 
      ttcgtaaat tccgataga aaattataa aaaagtagt 
      aaaaaagag taaaaaaag gtttgacga aaaaaattg 
      ttaaaatat agaactgaa aacaaaaga aagtaaatt 
      aaagccagg tgtttgaag aaaaacagg ctaagatat 
      aaaaaaata tattggaaa agaaaaata agaaaaata 
      taatgtatt ggtgtaaga agttaattt tttttacaa 
      ttgaatttt tcattttaa atacttatt tgatgacag 
      gattctgtt agatgaaga gaaaaaaga agaaaaaaa 
      taattaaaa ttaaaaaaa aacaagtcc aaagcgcca 
      actatattt gaaaagata aatagaatt tgaagcttt 
      ttgagttaa tttccaaaa aataaatcg tatcaagat 
      at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY