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Gene information
General information
TC007254 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6788740..6791843
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007254
      iBeetle screen iB_07205

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Fertility
      mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
      ovary -
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      17 larvae on slide and from them 13 is showing empty egg phenotype.

      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007254 RA
        MSPILILLL ITVASGVPI NMVQKAADN DFEKLDNYL 
        PKVGDFPKF HYSTFGGNT GSDELTNVI EFKSKENAK 
        SLGSVMDFV KDGASAGVD LLKDVGSVI TSTLHPETT 
        TEQETTTVP YEATTELEI TTKTLVQLD PVFLENVDD 
        NTNRTIRQD GDDDNDVEV DKDKSNDKE KESQESDEE 
        EEDQNMITG LLSAFLSGL SRPDGSVDV EAITGLLGS 
        LSTQNPDGS YDFNGLTEL LMSFFGGGG EEGGGSDFG 
        AFLGGLLGA FIKGVAQPP GAKGSGIFV GNLLTGILP 
        ALSGPAPTE EGQTEKPQP PLDSGGFLG GLLSSIAGS 
        SGSFSAGSS GGNNNANGG GGGSKFSLF KAIFSMITS 
        VFSSSSAAH
      CDS sequence
      • >TC007254 RA
        atgtctcca attttaatt ctcttgtta atcactgta 
        gcaagtggt gttccaata aatatggtc cagaaagca 
        gcagataac gatttcgag aaacttgat aattatttg 
        ccgaaagtt ggagatttc cccaaattt cattattct 
        acctttggg ggaaacacc gggagtgat gaattaacc 
        aacgtgatt gaattcaaa agcaaggaa aatgctaaa 
        agtttgggg agtgtcatg gactttgtc aaagacggt 
        gcgtctgct ggcgtggac ttgctaaaa gacgtcggc 
        agtgtgatc acatcaact ttgcaccct gaaaccacg 
        acggaacaa gaaactaca acggtacca tatgaagcc 
        accacagaa ttggaaatt acgacaaaa actttagtt 
        cagttggac ccagttttt ctggaaaat gttgacgac 
        aacacgaat agaacaata aggcaagac ggcgacgac 
        gacaatgac gtggaagtc gataaagac aaaagtaac 
        gataaagaa aaagaaagc caggagagt gatgaagag 
        gaggaagat cagaatatg atcactggc ctgctttcg 
        gcgtttttg agcgggtta agtcgaccc gacggtagt 
        gtcgacgtt gaggcaatt accggactg ttgggaagt 
        ctcagtact caaaaccct gacggttct tacgatttc 
        aacggcttg accgagctt ttgatgagc ttttttggg 
        ggaggaggc gaggaaggg ggcggttcc gactttggt 
        gctttttta ggaggtcta cttggagct tttattaaa 
        ggagtcgcc caacctcca ggagcgaaa ggatctggg 
        attttcgtt ggaaacctt ctaacagga attttgcca 
        gccttgagt ggaccggca cccaccgag gagggccag 
        accgagaag ccgcagccc cctttggac tccgggggc 
        ttcctgggg ggcctgctc tcctcgatt gccggaagc 
        agcggcagt ttcagcgct gggagcagc ggaggcaat 
        aataacgcc aacggcggg ggtggcggc tccaagttt 
        tcacttttc aaggcgatt ttttcgatg attacgtca 
        gttttttcg tcatcttcg gcggcacat tag
      mRNA sequence
      • >TC007254 RA
        ctataaaat aattaaact tagaaatta catagtatt 
        gtcgtacac gcatccatg agtccaata caataaatc 
        gctgttgtg gcactgaaa gaattggca actaatgac 
        tgtctggat ggaacccac atttttcaa taagtattc 
        gttgctaaa gactggaaa cggtaataa taaagacgt 
        tcatatcat ttaacatta ttgcaaaaa actctcttt 
        ttgatttac cgatatgac agttttcaa gtactcttc 
        acaaaacac acggcaccg aagaacagt tttgtttta 
        aacaagttg tgagcacag ttattttaa cattacgga 
        tatttatgg gacattagt agttttgtg gtcgcgatg 
        tctccaatt ttaattctc ttgttaatc actgtagca 
        agtggtgtt ccaataaat atggtccag aaagcagca 
        gataacgat ttcgagaaa cttgataat tatttgccg 
        aaagttgga gatttcccc aaatttcat tattctacc 
        tttggggga aacaccggg agtgatgaa ttaaccaac 
        gtgattgaa ttcaaaagc aaggaaaat gctaaaagt 
        ttggggagt gtcatggac tttgtcaaa gacggtgcg 
        tctgctggc gtggacttg ctaaaagac gtcggcagt 
        gtgatcaca tcaactttg caccctgaa accacgacg 
        gaacaagaa actacaacg gtaccatat gaagccacc 
        acagaattg gaaattacg acaaaaact ttagttcag 
        ttggaccca gtttttctg gaaaatgtt gacgacaac 
        acgaataga acaataagg caagacggc gacgacgac 
        aatgacgtg gaagtcgat aaagacaaa agtaacgat 
        aaagaaaaa gaaagccag gagagtgat gaagaggag 
        gaagatcag aatatgatc actggcctg ctttcggcg 
        tttttgagc gggttaagt cgacccgac ggtagtgtc 
        gacgttgag gcaattacc ggactgttg ggaagtctc 
        agtactcaa aaccctgac ggttcttac gatttcaac 
        ggcttgacc gagcttttg atgagcttt tttggggga 
        ggaggcgag gaagggggc ggttccgac tttggtgct 
        tttttagga ggtctactt ggagctttt attaaagga 
        gtcgcccaa cctccagga gcgaaagga tctgggatt 
        ttcgttgga aaccttcta acaggaatt ttgccagcc 
        ttgagtgga ccggcaccc accgaggag ggccagacc 
        gagaagccg cagccccct ttggactcc gggggcttc 
        ctggggggc ctgctctcc tcgattgcc ggaagcagc 
        ggcagtttc agcgctggg agcagcgga ggcaataat 
        aacgccaac ggcgggggt ggcggctcc aagttttca 
        cttttcaag gcgattttt tcgatgatt acgtcagtt 
        ttttcgtca tcttcggcg gcacattag acatgtggg 
        gctgtactt tttaataaa ttattccca agtgc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY