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Gene information
General information
TC032848
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007256
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6768003..6770972
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032848
    iBeetle screen iB_07204

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032848 RA
      MQNRLLIRS LLLFLITTC FALALQSSA 
      PNKNNVPNQ EIARDKRLI FKKKSSPGF 
      FRTLFSVVY EQWTDTKNT ISNVSRLIN 
      DNFAPENAP LKVSTTTVS SDPNATTTE 
      APFKITRSE FNRILRRNL RGLVRLFNI 
      ELQDALKVS EKNWAEYQK NASLEISKF 
      L
    CDS sequence
    • >TC032848 RA
      atgcagaat agattatta attagaagt ctgctgttg 
      tttttaata acaacctgt tttgctctg gcgctccag 
      tcgtcagcc cccaacaaa aacaatgtc ccgaaccaa 
      gaaattgcg cgcgataaa cgcctcatt tttaagaag 
      aaaagcagt ccgggattt tttcgaacc ctattttcg 
      gtagtatat gagcaatgg acagacacg aaaaataca 
      ataagtaac gtaagcaga ctaattaat gataatttc 
      gccccggaa aatgctccg ttgaaagtt tcgaccact 
      acggtgagt agtgatccg aatgctacg acaacggaa 
      gccccgttt aagataacc cggagtgag tttaaccgg 
      atcctccgg aggaattta cggggtttg gtccgcttg 
      ttcaatatc gaattacaa gacgctttg aaggtttcc 
      gaaaaaaat tgggccgag tatcagaaa aatgcgtcg 
      ttagaaatt tcgaaattt ttgtaa
    mRNA sequence
    • >TC032848 RA
      taaagttca agatcaagt ctatcattg accctgacg 
      cacacgtct aatttacaa tggtctagt gaaagttgc 
      atcaaaaat tacctaata ttcattccc aaatcttgc 
      aataataaa caacccaaa tttaatcga aattcgaaa 
      gtctctact gatagtagc taaaaagtg acagtttgc 
      aatgatata attctcgaa ttttgtcca actttcaaa 
      acaaaaatt atcacaaaa tagctaatt acgatgcac 
      aagtggtgt gagtcgaaa ttcgttggg caataccat 
      cgatttcca attacatcc gtctcttca ttactttct 
      tacttggag gcatgtgaa taataatgg aaaaggtcg 
      tcaaggctg gaaaatcga gcaaacctc ccaaaaatc 
      ccaaccaac ttgttatat cacggcttc tgccaacca 
      gtgagagta aatgcacac atgccttcg gtcctcagc 
      gtacgattt gtgacagaa aaggaaaac tctaccact 
      acgtgtaca gtttattca attcacttt caagtgccg 
      gtggattcc tgtaataac tattcgagc attctgatg 
      aaagaatgc cagtaggta aggtgtacg acgtagtgg 
      tggctagtt gacgagcaa tcagtgacg agtgataac 
      atggtgtag agataggat tttgaagca attttgcta 
      tgcagaata gattattaa ttagaagtc tgctgttgt 
      ttttaataa caacctgtt ttgctctgg cgctccagt 
      cgtcagccc ccaacaaaa acaatgtcc cgaaccaag 
      aaattgcgc gcgataaac gcctcattt ttaagaaga 
      aaagcagtc cgggatttt ttcgaaccc tattttcgg 
      tagtatatg agcaatgga cagacacga aaaatacaa 
      taagtaacg taagcagac taattaatg ataatttcg 
      ccccggaaa atgctccgt tgaaagttt cgaccacta 
      cggtgagta gtgatccga atgctacga caacggaag 
      ccccgttta agataaccc ggagtgagt ttaaccgga 
      tcctccgga ggaatttac ggggtttgg tccgcttgt 
      tcaatatcg aattacaag acgctttga aggtttccg 
      aaaaaaatt gggccgagt atcagaaaa atgcgtcgt 
      tagaaattt cgaaatttt tgtaaatgg gaaggaaat 
      gttgccaga ttgtctgtg ataaaatgt agaattatg 
      tggattgct caattaaag ttatggtcg ttagcttag 
      tttacaaaa tcatgacta tttattgct tgagtttta 
      gcgactgca ctattttgt aattttaat ttatttaat 
      ttgtaaata aatataaca aaagcgact tttatttct 
      atcattaac aagtaaaag ccggtaaaa gccaagttt 
      atctttttt tcatttagc ggaaaaatt tcaaacgca 
      aattttaac cttgtcctt ttgaataat tattttttg 
      aactatata attatgcac ccacgtaac tatttttac 
      tcaaaaaat gtccacact actttactt gctgtttct 
      agtgtcagt tgctcacaa tggggacgc tctcatccg 
      tagaaaact aattgagtc aagttatta cagttcgcc 
      gttaaattt tgtaaaaaa ttactagtg tttagtgtt 
      aatttaatc gcagatttt ttagttaaa attaccact 
      gacttatcg taaaggtac cttaggtgt gaatttctt 
      aggttttca cgctactgc aggtctttt tggcggttt 
      attggccga aacggctgg tttgggtgg ccctcatgc 
      tgaggctag tacgccttt tgggcaaag cgcgggtgc 
      tggtattgg tgtaagaag taaacttgg taaaaattc 
      aaagttttg atgaaggaa gttcgatgt aaaagaaat 
      ttttaggtt ctaattatg catatcttt actgttatt 
      ctcagttgg gcaaaagta aagctggga tggttttat 
      taggtggaa tggataagg ggagatgga aagtgaagc 
      ttcaaagca tcgaagatg ttgaagtgg gaaaatttc 
      tttaaaacg cttaattaa taatggaag tttatacaa 
      aatgtttcg tctaaaccc cacattcgg agtactgga 
      agttaaata agttgtaat ggtaatata ttgatctaa 
      aaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY