Status
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Gene information
General information
TC008205 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6626126..6632302
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008205
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07199

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008205 RA
      MDNYAYQPD DNGKPFAIT RYPSVPRPA NDYLVEVNS 
      RSKFLKKPK FFKILSAVL IAVIILLLI VIIILTVQY 
      YDKTYLSRI CTSEECLKS AANLKLSLD SSTNPCDDF 
      YQYTCGRWS KEHPNHGWF PTFSTFATI SEQILLSTE 
      EFLTSEETN DEPSPVTQA RNFYKSCMD EDALDDLGL 
      SVIFKYLKE VDLPSVPSF LTKSDDEIA EFHFDWLKS 
      DAVIKKTFA MDVLIGFVV DSNMLNQSE NAMYIGVPG 
      LRCPLPSPF KKTGGDKSE SAPDIDDLR RITNGNIIK 
      FVLSTLYRN VTSKELKED VLEQASDVL LNMTFEIDS 
      LVANFSSES NDLQSQIVK DFQTRINEI LRSDIKKDY 
      PQILEKYLG FLFTGTNVT IDFDNDFFL LSQNDVDYL 
      PRILSYVLT SSDIYLELY MWWVTVFAM IINTSSEIV 
      EYIVKQTAP FYTGTAVLR SRSLDCSAL VNSFMGMAV 
      SYGLADRTF SNKTKPKVE QMLYDIKSA FMEHVKTIS 
      WMDQKTRTA TLEKSKEMI SFIGYPDWL LDKGALEIY 
      YQGITLANN TYLDNMMSF IKLYVPSKL EVFRKINER 
      NWSTDPLSV NAFNSFTNN AINLPMAIL AFPIYHLGL 
      EVLNYGAMG SILGHELTH GFDNEGRKH DKFGNYKEW 
      WTHETIDTF EAKIECFVK QYDNFTLAG VEGHVQGKK 
      TLGENIADN GGLRHAYWA YRKYVDRNG HEPKLPGFE 
      NFTDNQLFF IAFGSIWCE TISPDDLES QIEYDEHSP 
      NKIRVIGSL QNSKEFAQA FQCPVGSRM NPNTNKCQV 
      W
    CDS sequence
    • >TC008205 RA
      atggataac tatgcttat cagccggac gacaacggc 
      aaacctttt gctatcaca agatatcct tcagtgcca 
      aggcccgcc aatgattat ttagtggaa gttaactct 
      aggtccaaa tttttaaag aagcccaag tttttcaaa 
      atattgtca gcggtactc attgctgta attatcctt 
      cttctaatc gtaatcata atcttgaca gtacagtac 
      tatgacaaa acatactta agcaggatt tgtacctca 
      gaggagtgt ctcaaatct gcagcaaat ctgaagctt 
      tcactagat tccagcacg aatccttgc gatgatttc 
      taccaatac acttgcggg cgctggtcg aaggaacac 
      ccaaaccat gggtggttt cccacattt tcaaccttt 
      gcaacaatt agcgaacaa atattatta tcaactgag 
      gagttttta acttcggag gaaaccaac gatgaacct 
      tcgcccgta acacaagct agaaatttc tacaagtct 
      tgcatggat gaagacgca ctcgatgat ttgggctta 
      tctgtaatt ttcaaatac ttgaaagag gtggacctg 
      ccctcagtt ccaagtttc ttgactaaa agtgatgat 
      gagatcgcc gaatttcac ttcgattgg ttaaaatcc 
      gacgctgtg ataaagaaa acttttgct atggatgtt 
      ttgatcggt tttgtggtt gactcaaat atgctcaac 
      cagtctgag aatgcgatg tatatcggg gtgcctggc 
      ctccggtgc cccttacct agtcctttt aaaaaaact 
      ggtggggac aagtccgaa agtgcgcct gatattgac 
      gatctccga aggataacc aacggaaat ataatcaaa 
      tttgttttg tccacattg tatagaaac gtgacgtca 
      aaggaactc aaggaggac gttcttgaa caagcgagt 
      gatgtgtta ttgaacatg acgtttgag attgattct 
      ctagttgca aatttttcc tcggagtca aacgacttg 
      caatcgcag attgttaag gactttcaa acgagaatc 
      aatgaaatt ttacggagt gatatcaaa aaggattat 
      ccccaaatt ttggaaaaa tacctcggg ttcttgttc 
      actggcact aacgtcacg attgatttc gacaacgat 
      ttcttcctc cttagtcaa aacgacgtg gactaccta 
      ccacggata ctgtcatac gtcctgacg agttcggac 
      atttatctt gaattatac atgtggtgg gtgacagtt 
      ttcgcaatg ataatcaat acttcgtca gaaattgtc 
      gaatacatt gttaagcag acggctccg ttttacact 
      ggcaccgct gtcttaaga tcgcggtcc ttggactgt 
      agcgcttta gttaacagc tttatgggg atggcggtt 
      agttacggc cttgcagat cgaaccttt tccaacaaa 
      acgaagcct aaggttgag caaatgttg tacgacatt 
      aaaagtgcg tttatggaa catgttaaa actataagt 
      tggatggat caaaaaacg cgaaccgcc actttggaa 
      aaaagcaaa gaaatgatc agttttatc ggatatccg 
      gattggttg ttggacaaa ggggccctt gaaatttac 
      tatcaaggc atcacgctg gctaataat acgtatttg 
      gacaacatg atgagcttt atcaagctt tatgttccg 
      agtaaattg gaagttttc aggaaaatt aatgaacgg 
      aattggagt actgaccct ctaagtgtc aacgcgttc 
      aattctttt acaaataat gctatcaat cttccaatg 
      gcaattttg gcatttccg atataccat ctcgggtta 
      gaagtttta aattatggt gctatggga tcgatttta 
      ggtcatgaa ttgactcac ggttttgac aatgaaggg 
      agaaaacac gacaaattt gggaattac aaggagtgg 
      tggacacac gaaaccatc gacacgttc gaagcaaaa 
      attgaatgt ttcgtcaag cagtacgat aattttaca 
      cttgcgggt gttgaggga catgtccaa gggaagaaa 
      actttaggt gaaaacatt gccgataac gggggactt 
      aggcacgct tattgggct tatcgtaaa tatgtcgat 
      cgaaacgga cacgagcct aagcttcca ggttttgag 
      aattttacg gataatcaa ttatttttt atagctttt 
      ggaagtatt tggtgtgag acaatttct cccgatgat 
      ttggaaagt cagattgaa tatgacgaa cattctcct 
      aataagata agagttata ggttcgtta caaaattcc 
      aaagaattt gctcaagcg tttcaatgt cctgtcggg 
      agtagaatg aacccaaat actaataaa tgtcaagtg 
      tggtga
    mRNA sequence
    • >TC008205 RA
      ccactgttg ccatcacac attccgcac caatagtaa 
      agacatttt tggccccgc agcccatct tgaagagtt 
      attatccag cgccactgg ctttcggac atgggtacc 
      attgaacca tcgctattt atttcattg tacataact 
      tattttata cctagaata atgtacaaa aattttcga 
      taataaata tttgcattt gaaccgtgt ttcttgtgt 
      tttgcaaca aaccgacac ctttatttg ctcgtggcg 
      ataaggttt agctcttta atgggcttt taaagtaca 
      tccatggta atcaagcac atatttgca attaaaacc 
      attattatt gtcactcac atacgtacg cgcaatgca 
      aaaggaagc aattagtgt atgaataat ttatattgt 
      ttaaaacat atcttgaat cgatattca gaagccatc 
      gatttgttt tattagtgg tacagtaac tgtgcgagt 
      agtttaccg gtaaagttc tcgcgcgca acatgacac 
      actttgcgt cagctaaaa ccaccctta gtagttttg 
      tggacaaca gacatggat aactatgct tatcagccg 
      gacgacaac ggcaaacct tttgctatc acaagatat 
      ccttcagtg ccaaggccc gccaatgat tatttagtg 
      gaagttaac tctaggtcc aaattttta aagaagccc 
      aagtttttc aaaatattg tcagcggta ctcattgct 
      gtaattatc cttcttcta atcgtaatc ataatcttg 
      acagtacag tactatgac aaaacatac ttaagcagg 
      atttgtacc tcagaggag tgtctcaaa tctgcagca 
      aatctgaag ctttcacta gattccagc acgaatcct 
      tgcgatgat ttctaccaa tacacttgc gggcgctgg 
      tcgaaggaa cacccaaac catgggtgg tttcccaca 
      ttttcaacc tttgcaaca attagcgaa caaatatta 
      ttatcaact gaggagttt ttaacttcg gaggaaacc 
      aacgatgaa ccttcgccc gtaacacaa gctagaaat 
      ttctacaag tcttgcatg gatgaagac gcactcgat 
      gatttgggc ttatctgta attttcaaa tacttgaaa 
      gaggtggac ctgccctca gttccaagt ttcttgact 
      aaaagtgat gatgagatc gccgaattt cacttcgat 
      tggttaaaa tccgacgct gtgataaag aaaactttt 
      gctatggat gttttgatc ggttttgtg gttgactca 
      aatatgctc aaccagtct gagaatgcg atgtatatc 
      ggggtgcct ggcctccgg tgcccctta cctagtcct 
      tttaaaaaa actggtggg gacaagtcc gaaagtgcg 
      cctgatatt gacgatctc cgaaggata accaacgga 
      aatataatc aaatttgtt ttgtccaca ttgtataga 
      aacgtgacg tcaaaggaa ctcaaggag gacgttctt 
      gaacaagcg agtgatgtg ttattgaac atgacgttt 
      gagattgat tctctagtt gcaaatttt tcctcggag 
      tcaaacgac ttgcaatcg cagattgtt aaggacttt 
      caaacgaga atcaatgaa attttacgg agtgatatc 
      aaaaaggat tatccccaa attttggaa aaatacctc 
      gggttcttg ttcactggc actaacgtc acgattgat 
      ttcgacaac gatttcttc ctccttagt caaaacgac 
      gtggactac ctaccacgg atactgtca tacgtcctg 
      acgagttcg gacatttat cttgaatta tacatgtgg 
      tgggtgaca gttttcgca atgataatc aatacttcg 
      tcagaaatt gtcgaatac attgttaag cagacggct 
      ccgttttac actggcacc gctgtctta agatcgcgg 
      tccttggac tgtagcgct ttagttaac agctttatg 
      gggatggcg gttagttac ggccttgca gatcgaacc 
      ttttccaac aaaacgaag cctaaggtt gagcaaatg 
      ttgtacgac attaaaagt gcgtttatg gaacatgtt 
      aaaactata agttggatg gatcaaaaa acgcgaacc 
      gccactttg gaaaaaagc aaagaaatg atcagtttt 
      atcggatat ccggattgg ttgttggac aaaggggcc 
      cttgaaatt tactatcaa ggcatcacg ctggctaat 
      aatacgtat ttggacaac atgatgagc tttatcaag 
      ctttatgtt ccgagtaaa ttggaagtt ttcaggaaa 
      attaatgaa cggaattgg agtactgac cctctaagt 
      gtcaacgcg ttcaattct tttacaaat aatgctatc 
      aatcttcca atggcaatt ttggcattt ccgatatac 
      catctcggg ttagaagtt ttaaattat ggtgctatg 
      ggatcgatt ttaggtcat gaattgact cacggtttt 
      gacaatgaa gggagaaaa cacgacaaa tttgggaat 
      tacaaggag tggtggaca cacgaaacc atcgacacg 
      ttcgaagca aaaattgaa tgtttcgtc aagcagtac 
      gataatttt acacttgcg ggtgttgag ggacatgtc 
      caagggaag aaaacttta ggtgaaaac attgccgat 
      aacggggga cttaggcac gcttattgg gcttatcgt 
      aaatatgtc gatcgaaac ggacacgag cctaagctt 
      ccaggtttt gagaatttt acggataat caattattt 
      tttatagct tttggaagt atttggtgt gagacaatt 
      tctcccgat gatttggaa agtcagatt gaatatgac 
      gaacattct cctaataag ataagagtt ataggttcg 
      ttacaaaat tccaaagaa tttgctcaa gcgtttcaa 
      tgtcctgtc gggagtaga atgaaccca aatactaat 
      aaatgtcaa gtgtggtga taaaagtaa aatgtgtga 
      tattttttt gaattgctt taattcaag tagaccaaa 
      atagctttc taatgtgtg ccttttgtg tgatattac 
      gtagataga gtatttttc aataaataa gtaattatc 
      gg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY