| General information | |||
| TC008205 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6626126..6632302 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008205 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07199 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC008205 RA
MDNYAYQPD DNGKPFAIT RYPSVPRPA NDYLVEVNS RSKFLKKPK FFKILSAVL IAVIILLLI VIIILTVQY YDKTYLSRI CTSEECLKS AANLKLSLD SSTNPCDDF YQYTCGRWS KEHPNHGWF PTFSTFATI SEQILLSTE EFLTSEETN DEPSPVTQA RNFYKSCMD EDALDDLGL SVIFKYLKE VDLPSVPSF LTKSDDEIA EFHFDWLKS DAVIKKTFA MDVLIGFVV DSNMLNQSE NAMYIGVPG LRCPLPSPF KKTGGDKSE SAPDIDDLR RITNGNIIK FVLSTLYRN VTSKELKED VLEQASDVL LNMTFEIDS LVANFSSES NDLQSQIVK DFQTRINEI LRSDIKKDY PQILEKYLG FLFTGTNVT IDFDNDFFL LSQNDVDYL PRILSYVLT SSDIYLELY MWWVTVFAM IINTSSEIV EYIVKQTAP FYTGTAVLR SRSLDCSAL VNSFMGMAV SYGLADRTF SNKTKPKVE QMLYDIKSA FMEHVKTIS WMDQKTRTA TLEKSKEMI SFIGYPDWL LDKGALEIY YQGITLANN TYLDNMMSF IKLYVPSKL EVFRKINER NWSTDPLSV NAFNSFTNN AINLPMAIL AFPIYHLGL EVLNYGAMG SILGHELTH GFDNEGRKH DKFGNYKEW WTHETIDTF EAKIECFVK QYDNFTLAG VEGHVQGKK TLGENIADN GGLRHAYWA YRKYVDRNG HEPKLPGFE NFTDNQLFF IAFGSIWCE TISPDDLES QIEYDEHSP NKIRVIGSL QNSKEFAQA FQCPVGSRM NPNTNKCQV W
- >TC008205 RA
atggataac tatgcttat cagccggac gacaacggc aaacctttt gctatcaca agatatcct tcagtgcca aggcccgcc aatgattat ttagtggaa gttaactct aggtccaaa tttttaaag aagcccaag tttttcaaa atattgtca gcggtactc attgctgta attatcctt cttctaatc gtaatcata atcttgaca gtacagtac tatgacaaa acatactta agcaggatt tgtacctca gaggagtgt ctcaaatct gcagcaaat ctgaagctt tcactagat tccagcacg aatccttgc gatgatttc taccaatac acttgcggg cgctggtcg aaggaacac ccaaaccat gggtggttt cccacattt tcaaccttt gcaacaatt agcgaacaa atattatta tcaactgag gagttttta acttcggag gaaaccaac gatgaacct tcgcccgta acacaagct agaaatttc tacaagtct tgcatggat gaagacgca ctcgatgat ttgggctta tctgtaatt ttcaaatac ttgaaagag gtggacctg ccctcagtt ccaagtttc ttgactaaa agtgatgat gagatcgcc gaatttcac ttcgattgg ttaaaatcc gacgctgtg ataaagaaa acttttgct atggatgtt ttgatcggt tttgtggtt gactcaaat atgctcaac cagtctgag aatgcgatg tatatcggg gtgcctggc ctccggtgc cccttacct agtcctttt aaaaaaact ggtggggac aagtccgaa agtgcgcct gatattgac gatctccga aggataacc aacggaaat ataatcaaa tttgttttg tccacattg tatagaaac gtgacgtca aaggaactc aaggaggac gttcttgaa caagcgagt gatgtgtta ttgaacatg acgtttgag attgattct ctagttgca aatttttcc tcggagtca aacgacttg caatcgcag attgttaag gactttcaa acgagaatc aatgaaatt ttacggagt gatatcaaa aaggattat ccccaaatt ttggaaaaa tacctcggg ttcttgttc actggcact aacgtcacg attgatttc gacaacgat ttcttcctc cttagtcaa aacgacgtg gactaccta ccacggata ctgtcatac gtcctgacg agttcggac atttatctt gaattatac atgtggtgg gtgacagtt ttcgcaatg ataatcaat acttcgtca gaaattgtc gaatacatt gttaagcag acggctccg ttttacact ggcaccgct gtcttaaga tcgcggtcc ttggactgt agcgcttta gttaacagc tttatgggg atggcggtt agttacggc cttgcagat cgaaccttt tccaacaaa acgaagcct aaggttgag caaatgttg tacgacatt aaaagtgcg tttatggaa catgttaaa actataagt tggatggat caaaaaacg cgaaccgcc actttggaa aaaagcaaa gaaatgatc agttttatc ggatatccg gattggttg ttggacaaa ggggccctt gaaatttac tatcaaggc atcacgctg gctaataat acgtatttg gacaacatg atgagcttt atcaagctt tatgttccg agtaaattg gaagttttc aggaaaatt aatgaacgg aattggagt actgaccct ctaagtgtc aacgcgttc aattctttt acaaataat gctatcaat cttccaatg gcaattttg gcatttccg atataccat ctcgggtta gaagtttta aattatggt gctatggga tcgatttta ggtcatgaa ttgactcac ggttttgac aatgaaggg agaaaacac gacaaattt gggaattac aaggagtgg tggacacac gaaaccatc gacacgttc gaagcaaaa attgaatgt ttcgtcaag cagtacgat aattttaca cttgcgggt gttgaggga catgtccaa gggaagaaa actttaggt gaaaacatt gccgataac gggggactt aggcacgct tattgggct tatcgtaaa tatgtcgat cgaaacgga cacgagcct aagcttcca ggttttgag aattttacg gataatcaa ttatttttt atagctttt ggaagtatt tggtgtgag acaatttct cccgatgat ttggaaagt cagattgaa tatgacgaa cattctcct aataagata agagttata ggttcgtta caaaattcc aaagaattt gctcaagcg tttcaatgt cctgtcggg agtagaatg aacccaaat actaataaa tgtcaagtg tggtga
- >TC008205 RA
ccactgttg ccatcacac attccgcac caatagtaa agacatttt tggccccgc agcccatct tgaagagtt attatccag cgccactgg ctttcggac atgggtacc attgaacca tcgctattt atttcattg tacataact tattttata cctagaata atgtacaaa aattttcga taataaata tttgcattt gaaccgtgt ttcttgtgt tttgcaaca aaccgacac ctttatttg ctcgtggcg ataaggttt agctcttta atgggcttt taaagtaca tccatggta atcaagcac atatttgca attaaaacc attattatt gtcactcac atacgtacg cgcaatgca aaaggaagc aattagtgt atgaataat ttatattgt ttaaaacat atcttgaat cgatattca gaagccatc gatttgttt tattagtgg tacagtaac tgtgcgagt agtttaccg gtaaagttc tcgcgcgca acatgacac actttgcgt cagctaaaa ccaccctta gtagttttg tggacaaca gacatggat aactatgct tatcagccg gacgacaac ggcaaacct tttgctatc acaagatat ccttcagtg ccaaggccc gccaatgat tatttagtg gaagttaac tctaggtcc aaattttta aagaagccc aagtttttc aaaatattg tcagcggta ctcattgct gtaattatc cttcttcta atcgtaatc ataatcttg acagtacag tactatgac aaaacatac ttaagcagg atttgtacc tcagaggag tgtctcaaa tctgcagca aatctgaag ctttcacta gattccagc acgaatcct tgcgatgat ttctaccaa tacacttgc gggcgctgg tcgaaggaa cacccaaac catgggtgg tttcccaca ttttcaacc tttgcaaca attagcgaa caaatatta ttatcaact gaggagttt ttaacttcg gaggaaacc aacgatgaa ccttcgccc gtaacacaa gctagaaat ttctacaag tcttgcatg gatgaagac gcactcgat gatttgggc ttatctgta attttcaaa tacttgaaa gaggtggac ctgccctca gttccaagt ttcttgact aaaagtgat gatgagatc gccgaattt cacttcgat tggttaaaa tccgacgct gtgataaag aaaactttt gctatggat gttttgatc ggttttgtg gttgactca aatatgctc aaccagtct gagaatgcg atgtatatc ggggtgcct ggcctccgg tgcccctta cctagtcct tttaaaaaa actggtggg gacaagtcc gaaagtgcg cctgatatt gacgatctc cgaaggata accaacgga aatataatc aaatttgtt ttgtccaca ttgtataga aacgtgacg tcaaaggaa ctcaaggag gacgttctt gaacaagcg agtgatgtg ttattgaac atgacgttt gagattgat tctctagtt gcaaatttt tcctcggag tcaaacgac ttgcaatcg cagattgtt aaggacttt caaacgaga atcaatgaa attttacgg agtgatatc aaaaaggat tatccccaa attttggaa aaatacctc gggttcttg ttcactggc actaacgtc acgattgat ttcgacaac gatttcttc ctccttagt caaaacgac gtggactac ctaccacgg atactgtca tacgtcctg acgagttcg gacatttat cttgaatta tacatgtgg tgggtgaca gttttcgca atgataatc aatacttcg tcagaaatt gtcgaatac attgttaag cagacggct ccgttttac actggcacc gctgtctta agatcgcgg tccttggac tgtagcgct ttagttaac agctttatg gggatggcg gttagttac ggccttgca gatcgaacc ttttccaac aaaacgaag cctaaggtt gagcaaatg ttgtacgac attaaaagt gcgtttatg gaacatgtt aaaactata agttggatg gatcaaaaa acgcgaacc gccactttg gaaaaaagc aaagaaatg atcagtttt atcggatat ccggattgg ttgttggac aaaggggcc cttgaaatt tactatcaa ggcatcacg ctggctaat aatacgtat ttggacaac atgatgagc tttatcaag ctttatgtt ccgagtaaa ttggaagtt ttcaggaaa attaatgaa cggaattgg agtactgac cctctaagt gtcaacgcg ttcaattct tttacaaat aatgctatc aatcttcca atggcaatt ttggcattt ccgatatac catctcggg ttagaagtt ttaaattat ggtgctatg ggatcgatt ttaggtcat gaattgact cacggtttt gacaatgaa gggagaaaa cacgacaaa tttgggaat tacaaggag tggtggaca cacgaaacc atcgacacg ttcgaagca aaaattgaa tgtttcgtc aagcagtac gataatttt acacttgcg ggtgttgag ggacatgtc caagggaag aaaacttta ggtgaaaac attgccgat aacggggga cttaggcac gcttattgg gcttatcgt aaatatgtc gatcgaaac ggacacgag cctaagctt ccaggtttt gagaatttt acggataat caattattt tttatagct tttggaagt atttggtgt gagacaatt tctcccgat gatttggaa agtcagatt gaatatgac gaacattct cctaataag ataagagtt ataggttcg ttacaaaat tccaaagaa tttgctcaa gcgtttcaa tgtcctgtc gggagtaga atgaaccca aatactaat aaatgtcaa gtgtggtga taaaagtaa aatgtgtga tattttttt gaattgctt taattcaag tagaccaaa atagctttc taatgtgtg ccttttgtg tgatattac gtagataga gtatttttc aataaataa gtaattatc gg
