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Gene information
General information
TC008205 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6626126..6632302
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008205
    iBeetle screen iB_07199

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008205 RA
      MDNYAYQPD DNGKPFAIT RYPSVPRPA NDYLVEVNS 
RSKFLKKPK FFKILSAVL IAVIILLLI VIIILTVQY 
YDKTYLSRI CTSEECLKS AANLKLSLD SSTNPCDDF 
YQYTCGRWS KEHPNHGWF PTFSTFATI SEQILLSTE 
EFLTSEETN DEPSPVTQA RNFYKSCMD EDALDDLGL 
SVIFKYLKE VDLPSVPSF LTKSDDEIA EFHFDWLKS 
DAVIKKTFA MDVLIGFVV DSNMLNQSE NAMYIGVPG 
LRCPLPSPF KKTGGDKSE SAPDIDDLR RITNGNIIK 
FVLSTLYRN VTSKELKED VLEQASDVL LNMTFEIDS 
LVANFSSES NDLQSQIVK DFQTRINEI LRSDIKKDY 
PQILEKYLG FLFTGTNVT IDFDNDFFL LSQNDVDYL 
PRILSYVLT SSDIYLELY MWWVTVFAM IINTSSEIV 
EYIVKQTAP FYTGTAVLR SRSLDCSAL VNSFMGMAV 
SYGLADRTF SNKTKPKVE QMLYDIKSA FMEHVKTIS 
WMDQKTRTA TLEKSKEMI SFIGYPDWL LDKGALEIY 
YQGITLANN TYLDNMMSF IKLYVPSKL EVFRKINER 
NWSTDPLSV NAFNSFTNN AINLPMAIL AFPIYHLGL 
EVLNYGAMG SILGHELTH GFDNEGRKH DKFGNYKEW 
WTHETIDTF EAKIECFVK QYDNFTLAG VEGHVQGKK 
TLGENIADN GGLRHAYWA YRKYVDRNG HEPKLPGFE 
NFTDNQLFF IAFGSIWCE TISPDDLES QIEYDEHSP 
NKIRVIGSL QNSKEFAQA FQCPVGSRM NPNTNKCQV 
W
    CDS sequence
    • >TC008205 RA
      atggataac tatgcttat cagccggac gacaacggc 
aaacctttt gctatcaca agatatcct tcagtgcca 
aggcccgcc aatgattat ttagtggaa gttaactct 
aggtccaaa tttttaaag aagcccaag tttttcaaa 
atattgtca gcggtactc attgctgta attatcctt 
cttctaatc gtaatcata atcttgaca gtacagtac 
tatgacaaa acatactta agcaggatt tgtacctca 
gaggagtgt ctcaaatct gcagcaaat ctgaagctt 
tcactagat tccagcacg aatccttgc gatgatttc 
taccaatac acttgcggg cgctggtcg aaggaacac 
ccaaaccat gggtggttt cccacattt tcaaccttt 
gcaacaatt agcgaacaa atattatta tcaactgag 
gagttttta acttcggag gaaaccaac gatgaacct 
tcgcccgta acacaagct agaaatttc tacaagtct 
tgcatggat gaagacgca ctcgatgat ttgggctta 
tctgtaatt ttcaaatac ttgaaagag gtggacctg 
ccctcagtt ccaagtttc ttgactaaa agtgatgat 
gagatcgcc gaatttcac ttcgattgg ttaaaatcc 
gacgctgtg ataaagaaa acttttgct atggatgtt 
ttgatcggt tttgtggtt gactcaaat atgctcaac 
cagtctgag aatgcgatg tatatcggg gtgcctggc 
ctccggtgc cccttacct agtcctttt aaaaaaact 
ggtggggac aagtccgaa agtgcgcct gatattgac 
gatctccga aggataacc aacggaaat ataatcaaa 
tttgttttg tccacattg tatagaaac gtgacgtca 
aaggaactc aaggaggac gttcttgaa caagcgagt 
gatgtgtta ttgaacatg acgtttgag attgattct 
ctagttgca aatttttcc tcggagtca aacgacttg 
caatcgcag attgttaag gactttcaa acgagaatc 
aatgaaatt ttacggagt gatatcaaa aaggattat 
ccccaaatt ttggaaaaa tacctcggg ttcttgttc 
actggcact aacgtcacg attgatttc gacaacgat 
ttcttcctc cttagtcaa aacgacgtg gactaccta 
ccacggata ctgtcatac gtcctgacg agttcggac 
atttatctt gaattatac atgtggtgg gtgacagtt 
ttcgcaatg ataatcaat acttcgtca gaaattgtc 
gaatacatt gttaagcag acggctccg ttttacact 
ggcaccgct gtcttaaga tcgcggtcc ttggactgt 
agcgcttta gttaacagc tttatgggg atggcggtt 
agttacggc cttgcagat cgaaccttt tccaacaaa 
acgaagcct aaggttgag caaatgttg tacgacatt 
aaaagtgcg tttatggaa catgttaaa actataagt 
tggatggat caaaaaacg cgaaccgcc actttggaa 
aaaagcaaa gaaatgatc agttttatc ggatatccg 
gattggttg ttggacaaa ggggccctt gaaatttac 
tatcaaggc atcacgctg gctaataat acgtatttg 
gacaacatg atgagcttt atcaagctt tatgttccg 
agtaaattg gaagttttc aggaaaatt aatgaacgg 
aattggagt actgaccct ctaagtgtc aacgcgttc 
aattctttt acaaataat gctatcaat cttccaatg 
gcaattttg gcatttccg atataccat ctcgggtta 
gaagtttta aattatggt gctatggga tcgatttta 
ggtcatgaa ttgactcac ggttttgac aatgaaggg 
agaaaacac gacaaattt gggaattac aaggagtgg 
tggacacac gaaaccatc gacacgttc gaagcaaaa 
attgaatgt ttcgtcaag cagtacgat aattttaca 
cttgcgggt gttgaggga catgtccaa gggaagaaa 
actttaggt gaaaacatt gccgataac gggggactt 
aggcacgct tattgggct tatcgtaaa tatgtcgat 
cgaaacgga cacgagcct aagcttcca ggttttgag 
aattttacg gataatcaa ttatttttt atagctttt 
ggaagtatt tggtgtgag acaatttct cccgatgat 
ttggaaagt cagattgaa tatgacgaa cattctcct 
aataagata agagttata ggttcgtta caaaattcc 
aaagaattt gctcaagcg tttcaatgt cctgtcggg 
agtagaatg aacccaaat actaataaa tgtcaagtg 
tggtga
    mRNA sequence
    • >TC008205 RA
      ccactgttg ccatcacac attccgcac caatagtaa 
agacatttt tggccccgc agcccatct tgaagagtt 
attatccag cgccactgg ctttcggac atgggtacc 
attgaacca tcgctattt atttcattg tacataact 
tattttata cctagaata atgtacaaa aattttcga 
taataaata tttgcattt gaaccgtgt ttcttgtgt 
tttgcaaca aaccgacac ctttatttg ctcgtggcg 
ataaggttt agctcttta atgggcttt taaagtaca 
tccatggta atcaagcac atatttgca attaaaacc 
attattatt gtcactcac atacgtacg cgcaatgca 
aaaggaagc aattagtgt atgaataat ttatattgt 
ttaaaacat atcttgaat cgatattca gaagccatc 
gatttgttt tattagtgg tacagtaac tgtgcgagt 
agtttaccg gtaaagttc tcgcgcgca acatgacac 
actttgcgt cagctaaaa ccaccctta gtagttttg 
tggacaaca gacatggat aactatgct tatcagccg 
gacgacaac ggcaaacct tttgctatc acaagatat 
ccttcagtg ccaaggccc gccaatgat tatttagtg 
gaagttaac tctaggtcc aaattttta aagaagccc 
aagtttttc aaaatattg tcagcggta ctcattgct 
gtaattatc cttcttcta atcgtaatc ataatcttg 
acagtacag tactatgac aaaacatac ttaagcagg 
atttgtacc tcagaggag tgtctcaaa tctgcagca 
aatctgaag ctttcacta gattccagc acgaatcct 
tgcgatgat ttctaccaa tacacttgc gggcgctgg 
tcgaaggaa cacccaaac catgggtgg tttcccaca 
ttttcaacc tttgcaaca attagcgaa caaatatta 
ttatcaact gaggagttt ttaacttcg gaggaaacc 
aacgatgaa ccttcgccc gtaacacaa gctagaaat 
ttctacaag tcttgcatg gatgaagac gcactcgat 
gatttgggc ttatctgta attttcaaa tacttgaaa 
gaggtggac ctgccctca gttccaagt ttcttgact 
aaaagtgat gatgagatc gccgaattt cacttcgat 
tggttaaaa tccgacgct gtgataaag aaaactttt 
gctatggat gttttgatc ggttttgtg gttgactca 
aatatgctc aaccagtct gagaatgcg atgtatatc 
ggggtgcct ggcctccgg tgcccctta cctagtcct 
tttaaaaaa actggtggg gacaagtcc gaaagtgcg 
cctgatatt gacgatctc cgaaggata accaacgga 
aatataatc aaatttgtt ttgtccaca ttgtataga 
aacgtgacg tcaaaggaa ctcaaggag gacgttctt 
gaacaagcg agtgatgtg ttattgaac atgacgttt 
gagattgat tctctagtt gcaaatttt tcctcggag 
tcaaacgac ttgcaatcg cagattgtt aaggacttt 
caaacgaga atcaatgaa attttacgg agtgatatc 
aaaaaggat tatccccaa attttggaa aaatacctc 
gggttcttg ttcactggc actaacgtc acgattgat 
ttcgacaac gatttcttc ctccttagt caaaacgac 
gtggactac ctaccacgg atactgtca tacgtcctg 
acgagttcg gacatttat cttgaatta tacatgtgg 
tgggtgaca gttttcgca atgataatc aatacttcg 
tcagaaatt gtcgaatac attgttaag cagacggct 
ccgttttac actggcacc gctgtctta agatcgcgg 
tccttggac tgtagcgct ttagttaac agctttatg 
gggatggcg gttagttac ggccttgca gatcgaacc 
ttttccaac aaaacgaag cctaaggtt gagcaaatg 
ttgtacgac attaaaagt gcgtttatg gaacatgtt 
aaaactata agttggatg gatcaaaaa acgcgaacc 
gccactttg gaaaaaagc aaagaaatg atcagtttt 
atcggatat ccggattgg ttgttggac aaaggggcc 
cttgaaatt tactatcaa ggcatcacg ctggctaat 
aatacgtat ttggacaac atgatgagc tttatcaag 
ctttatgtt ccgagtaaa ttggaagtt ttcaggaaa 
attaatgaa cggaattgg agtactgac cctctaagt 
gtcaacgcg ttcaattct tttacaaat aatgctatc 
aatcttcca atggcaatt ttggcattt ccgatatac 
catctcggg ttagaagtt ttaaattat ggtgctatg 
ggatcgatt ttaggtcat gaattgact cacggtttt 
gacaatgaa gggagaaaa cacgacaaa tttgggaat 
tacaaggag tggtggaca cacgaaacc atcgacacg 
ttcgaagca aaaattgaa tgtttcgtc aagcagtac 
gataatttt acacttgcg ggtgttgag ggacatgtc 
caagggaag aaaacttta ggtgaaaac attgccgat 
aacggggga cttaggcac gcttattgg gcttatcgt 
aaatatgtc gatcgaaac ggacacgag cctaagctt 
ccaggtttt gagaatttt acggataat caattattt 
tttatagct tttggaagt atttggtgt gagacaatt 
tctcccgat gatttggaa agtcagatt gaatatgac 
gaacattct cctaataag ataagagtt ataggttcg 
ttacaaaat tccaaagaa tttgctcaa gcgtttcaa 
tgtcctgtc gggagtaga atgaaccca aatactaat 
aaatgtcaa gtgtggtga taaaagtaa aatgtgtga 
tattttttt gaattgctt taattcaag tagaccaaa 
atagctttc taatgtgtg ccttttgtg tgatattac 
gtagataga gtatttttc aataaataa gtaattatc 
gg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY