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Gene information
General information
TC008182 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6170928..6172168
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008182
    iBeetle screen iB_07191

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008182 RA
      MTGDPNQEN RPSVFGFEP YPSLNVQPI 
      DWSRIPFEP SAAPVIPQG QFNPSLSYQ 
      QLPSSIGHF SFCASQTPH PAQFPFPSS 
      PPIENNVLA HHSLPSVRC KRKTESPPL 
      QPCKQHITE EKMAEHMSR LHISSETAT 
      SSRETEGDR TRRLYMCEE MRKLQSDSI 
      LPQSLLSKI QRPCTALVL WTPPRRLIP 
      LENTENENN NEEEAPDQN KMAMEADMN 
      NMDLDNV
    CDS sequence
    • >TC008182 RA
      atgacagga gaccctaac caagaaaac cgtccgtca 
      gttttcggt tttgaaccg tacccttcc ctcaacgtg 
      cagcccatc gattggagt cgcatccca ttcgagcct 
      tcggcagcc ccagttatc ccccaggga cagtttaac 
      ccgtcgtta agttaccaa cagctacct tcaagtata 
      ggacatttc tcgttttgt gcatcccag accccacat 
      ccggctcag ttcccgttt ccttccagc cctccaatc 
      gaaaataat gtactggca caccattcc ctaccttca 
      gtgcgctgc aagagaaaa acggagtcg ccaccgtta 
      cagccttgc aagcaacat atcacagag gagaagatg 
      gcagagcac atgtctcgc ttgcacata agttccgaa 
      acggcgact tcgtcgcgg gaaacagaa ggggacaga 
      actaggcgt ttgtacatg tgtgaggag atgaggaaa 
      ttgcaaagc gattcgatt cttccccag tctctgttg 
      tccaaaatt caacgacct tgtacagcc ctggtcttg 
      tggacgccg cctcggcgg ttaattcct ttagaaaac 
      acagagaat gaaaataat aacgaggaa gaggcccct 
      gatcagaat aagatggcc atggaggcc gacatgaac 
      aatatggac ctagataat gtttaa
    mRNA sequence
    • >TC008182 RA
      agtgtcaaa cagctgatt agaagttgc gtcgactca 
      ggagctttg tggtcactt caaacaaaa aataaacaa 
      ttattcgaa gtgtttttg tgtattttc aatccagtg 
      ccggttttt ttcgcataa gtgcccagt tagtggttg 
      catgtatga ataacatga caggagacc ctaaccaag 
      aaaaccgtc cgtcagttt tcggttttg aaccgtacc 
      cttccctca acgtgcagc ccatcgatt ggagtcgca 
      tcccattcg agccttcgg cagccccag ttatccccc 
      agggacagt ttaacccgt cgttaagtt accaacagc 
      taccttcaa gtataggac atttctcgt tttgtgcat 
      cccagaccc cacatccgg ctcagttcc cgtttcctt 
      ccagccctc caatcgaaa ataatgtac tggcacacc 
      attccctac cttcagtgc gctgcaaga gaaaaacgg 
      agtcgccac cgttacagc cttgcaagc aacatatca 
      cagaggaga agatggcag agcacatgt ctcgcttgc 
      acataagtt ccgaaacgg cgacttcgt cgcgggaaa 
      cagaagggg acagaacta ggcgtttgt acatgtgtg 
      aggagatga ggaaattgc aaagcgatt cgattcttc 
      cccagtctc tgttgtcca aaattcaac gaccttgta 
      cagccctgg tcttgtgga cgccgcctc ggcggttaa 
      ttcctttag aaaacacag agaatgaaa ataataacg 
      aggaagagg cccctgatc agaataaga tggccatgg 
      aggccgaca tgaacaata tggacctag ataatgttt 
      aagttcttc ttccaatga ttattctca aagactgat 
      gatttttat tgagatatt gcgttccta gccttattt 
      ttttatcgt gaggtggaa ttaatggac gaagtggtg 
      ttacacaga ccagttttt tgcaatcga gctttaagt 
      aatttataa ttttttaag aatctaacg aattgacct 
      ataatggat ttaaggatt gctcacgtt tgttcgttt 
      accgttttt ttactacta tacattgct gtattagac 
      gtaagtgtc tttttttag tttagtttt gtagtgatt 
      tgttaattc ttaacattt tttggttgt gttttgtaa 
      tggcagtgc aattggcca caataaagc gattactct 
      taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY