Status
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Gene information
General information
TC007327 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6140556..6143036
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007327
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07189

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007327 RA
      MTDLESPKK NNDCYFYYY STCAKGDNC TFRHEPSAL 
      GCETMCSFW KEGKCLNVH CNFRHMELR KNRKAIPCY 
      WESQPGGCL KPHCPFLHQ SPKPAAANE SHESKTDSG 
      TESADNSMN EKFSHLSEK GFKNAPVDS LVVNFEEES 
      DNESAPTNS PIKSNHRAV KVKTLEEIK LEKIQAESA 
      AYYSYPEDD GEDLRQRIW ERLSTKNPG FGLKRQHET 
      NITGPLSKK PKKTETDVS EIKIKTLDE IRAERAKKT 
      SQNSDAINI CDSCVSSST EDTQNEVKK AKIKLQREP 
      AYIQEKKEQ LNETKEVVD KTSDSDVDM SQSNNNKTA 
      DEILLLQDD DDNCNVSLK EEERLLNDL DDFLED
    • >TC007327 RB
      MTEKYYCLD NFFKKMTDL ESPKKNNDC YFYYYSTCA 
      KGDNCTFRH EPSALGCET MCSFWKEGK CLNVHCNFR 
      HMELRKNRK AIPCYWESQ PGGCLKPHC PFLHQSPKP 
      AAANESHES KTDSGTESA DNSMNEKFS HLSEKGFKN 
      APVDSLVVN FEEESDNES APTNSPIKS NHRAVKVKT 
      LEEIKLEKI QAESAAYYS YPEDDGEDL RQRIWERLS 
      TKNPGFGLK RQHETNITG PLSKKPKKT ETDVSEIKI 
      KTLDEIRAE RAKKTSQNS DAINICDSC VSSSTEDTQ 
      NEVKKAKIK LQREPAYIQ EKKEQLNET KEVVDKTSD 
      SDVDMSQSN NNKTADEIL LLQDDDDNC NVSLKEEER 
      LLNDLDDFL ED
    CDS sequence
    • >TC007327 RA
      atgacggat ttggagtcc ccgaagaag aacaatgat 
      tgctatttc tactattac tcgacgtgt gcgaagggt 
      gacaactgc acgtttcgc cacgaacca tctgcttta 
      ggctgtgaa actatgtgt tccttctgg aaggaaggt 
      aaatgctta aatgtccac tgcaatttc aggcacatg 
      gaattgaga aaaaaccgc aaagcgata ccttgttac 
      tgggagtcg caacctggc ggctgtcta aagcctcac 
      tgtcccttc ctgcatcag agccccaaa cctgcagct 
      gctaacgaa tctcatgaa agtaaaaca gattctggg 
      acagaatct gcggataat tcaatgaac gaaaaattt 
      agccatttg agtgagaag ggtttcaag aacgcgccc 
      gttgactcc ctggttgtt aattttgaa gaagaatct 
      gacaatgaa tcagcgcca accaattcg ccaattaag 
      tccaaccat cgcgctgtg aaagttaaa actttggaa 
      gaaattaaa ctggaaaaa attcaagcc gaatctgcc 
      gcttactac tcatatcca gaagacgac ggtgaggac 
      ttgcgccag aggatatgg gaacgactt tcaacgaaa 
      aatcccgga tttggttta aagagacaa cacgaaaca 
      aacatcaca gggccgctc tcaaaaaaa ccgaagaaa 
      acagaaaca gacgtcagt gaaattaag attaagact 
      ttggacgaa atccgggct gaacgagca aagaaaacc 
      agtcaaaat tcagatgca atcaatatt tgtgatagt 
      tgcgttagt agtagtact gaagatact cagaatgag 
      gtgaaaaag gcaaaaata aaactacaa agggagcca 
      gcctatatt caagaaaag aaagaacag ttgaatgaa 
      accaaagaa gttgttgac aaaactagt gatagtgat 
      gttgatatg agccagagt aataataat aagacagct 
      gacgaaatt ttgctcctg caggatgac gatgataat 
      tgtaatgtt agtttgaag gaggaggag aggctttta 
      aatgatttg gacgatttt ttggaagac tga
    • >TC007327 RB
      atgacagaa aagtattat tgtctagac aatttcttc 
      aagaaaatg acggatttg gagtccccg aagaagaac 
      aatgattgc tatttctac tattactcg acgtgtgcg 
      aagggtgac aactgcacg tttcgccac gaaccatct 
      gctttaggc tgtgaaact atgtgttcc ttctggaag 
      gaaggtaaa tgcttaaat gtccactgc aatttcagg 
      cacatggaa ttgagaaaa aaccgcaaa gcgatacct 
      tgttactgg gagtcgcaa cctggcggc tgtctaaag 
      cctcactgt cccttcctg catcagagc cccaaacct 
      gcagctgct aacgaatct catgaaagt aaaacagat 
      tctgggaca gaatctgcg gataattca atgaacgaa 
      aaatttagc catttgagt gagaagggt ttcaagaac 
      gcgcccgtt gactccctg gttgttaat tttgaagaa 
      gaatctgac aatgaatca gcgccaacc aattcgcca 
      attaagtcc aaccatcgc gctgtgaaa gttaaaact 
      ttggaagaa attaaactg gaaaaaatt caagccgaa 
      tctgccgct tactactca tatccagaa gacgacggt 
      gaggacttg cgccagagg atatgggaa cgactttca 
      acgaaaaat cccggattt ggtttaaag agacaacac 
      gaaacaaac atcacaggg ccgctctca aaaaaaccg 
      aagaaaaca gaaacagac gtcagtgaa attaagatt 
      aagactttg gacgaaatc cgggctgaa cgagcaaag 
      aaaaccagt caaaattca gatgcaatc aatatttgt 
      gatagttgc gttagtagt agtactgaa gatactcag 
      aatgaggtg aaaaaggca aaaataaaa ctacaaagg 
      gagccagcc tatattcaa gaaaagaaa gaacagttg 
      aatgaaacc aaagaagtt gttgacaaa actagtgat 
      agtgatgtt gatatgagc cagagtaat aataataag 
      acagctgac gaaattttg ctcctgcag gatgacgat 
      gataattgt aatgttagt ttgaaggag gaggagagg 
      cttttaaat gatttggac gattttttg gaagactga
    mRNA sequence
    • >TC007327 RA
      ttggcgaca aaaaataaa aaggtcacg tattttctt 
      cgaagtcta gacaatttc ttcaagaaa atgacggat 
      ttggagtcc ccgaagaag aacaatgat tgctatttc 
      tactattac tcgacgtgt gcgaagggt gacaactgc 
      acgtttcgc cacgaacca tctgcttta ggctgtgaa 
      actatgtgt tccttctgg aaggaaggt aaatgctta 
      aatgtccac tgcaatttc aggcacatg gaattgaga 
      aaaaaccgc aaagcgata ccttgttac tgggagtcg 
      caacctggc ggctgtcta aagcctcac tgtcccttc 
      ctgcatcag agccccaaa cctgcagct gctaacgaa 
      tctcatgaa agtaaaaca gattctggg acagaatct 
      gcggataat tcaatgaac gaaaaattt agccatttg 
      agtgagaag ggtttcaag aacgcgccc gttgactcc 
      ctggttgtt aattttgaa gaagaatct gacaatgaa 
      tcagcgcca accaattcg ccaattaag tccaaccat 
      cgcgctgtg aaagttaaa actttggaa gaaattaaa 
      ctggaaaaa attcaagcc gaatctgcc gcttactac 
      tcatatcca gaagacgac ggtgaggac ttgcgccag 
      aggatatgg gaacgactt tcaacgaaa aatcccgga 
      tttggttta aagagacaa cacgaaaca aacatcaca 
      gggccgctc tcaaaaaaa ccgaagaaa acagaaaca 
      gacgtcagt gaaattaag attaagact ttggacgaa 
      atccgggct gaacgagca aagaaaacc agtcaaaat 
      tcagatgca atcaatatt tgtgatagt tgcgttagt 
      agtagtact gaagatact cagaatgag gtgaaaaag 
      gcaaaaata aaactacaa agggagcca gcctatatt 
      caagaaaag aaagaacag ttgaatgaa accaaagaa 
      gttgttgac aaaactagt gatagtgat gttgatatg 
      agccagagt aataataat aagacagct gacgaaatt 
      ttgctcctg caggatgac gatgataat tgtaatgtt 
      agtttgaag gaggaggag aggctttta aatgatttg 
      gacgatttt ttggaagac tgagttagg gacaccgtg 
      tcattatta tgttaagca ttaatttta atttaacga 
      gaagcagtt ttatttttc atgtttaca ttatcagcg 
      ttgtacaaa atatatttt aagagtttt tcaaaataa 
      atatttaga taatta
    • >TC007327 RB
      agatttcct gttggcgac aaaaaataa aaaggtcac 
      gtattttct tcgaaggta agctgtcag tttgttttt 
      ttacaagaa gtgataatt ttttgttaa aaagtttgg 
      ggtaaatag gcttgaaaa tcccgtggt gttatttgt 
      caaaaaatc caaattttt cgtgaagca acgcaatca 
      tgacagaaa agtattatt gtctagaca atttcttca 
      agaaaatga cggatttgg agtccccga agaagaaca 
      atgattgct atttctact attactcga cgtgtgcga 
      agggtgaca actgcacgt ttcgccacg aaccatctg 
      ctttaggct gtgaaacta tgtgttcct tctggaagg 
      aaggtaaat gcttaaatg tccactgca atttcaggc 
      acatggaat tgagaaaaa accgcaaag cgatacctt 
      gttactggg agtcgcaac ctggcggct gtctaaagc 
      ctcactgtc ccttcctgc atcagagcc ccaaacctg 
      cagctgcta acgaatctc atgaaagta aaacagatt 
      ctgggacag aatctgcgg ataattcaa tgaacgaaa 
      aatttagcc atttgagtg agaagggtt tcaagaacg 
      cgcccgttg actccctgg ttgttaatt ttgaagaag 
      aatctgaca atgaatcag cgccaacca attcgccaa 
      ttaagtcca accatcgcg ctgtgaaag ttaaaactt 
      tggaagaaa ttaaactgg aaaaaattc aagccgaat 
      ctgccgctt actactcat atccagaag acgacggtg 
      aggacttgc gccagagga tatgggaac gactttcaa 
      cgaaaaatc ccggatttg gtttaaaga gacaacacg 
      aaacaaaca tcacagggc cgctctcaa aaaaaccga 
      agaaaacag aaacagacg tcagtgaaa ttaagatta 
      agactttgg acgaaatcc gggctgaac gagcaaaga 
      aaaccagtc aaaattcag atgcaatca atatttgtg 
      atagttgcg ttagtagta gtactgaag atactcaga 
      atgaggtga aaaaggcaa aaataaaac tacaaaggg 
      agccagcct atattcaag aaaagaaag aacagttga 
      atgaaacca aagaagttg ttgacaaaa ctagtgata 
      gtgatgttg atatgagcc agagtaata ataataaga 
      cagctgacg aaattttgc tcctgcagg atgacgatg 
      ataattgta atgttagtt tgaaggagg aggagaggc 
      ttttaaatg atttggacg attttttgg aagactgag 
      ttagggaca ccgtgtcat tattatgtt aagcattaa 
      ttttaattt aacgagaag cagttttat ttttcatgt 
      ttacattat cagcgttgt acaaaatat attttaaga 
      gtttttcaa aataaatat ttagataat ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY