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Gene information
General information
TC032832
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008176
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6136608..6140202
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032832
    iBeetle screen iB_07188

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content opaque - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands size increased - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    gena triplet gena triplet dorsal not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032832 RA
      MPTAAPALV TLSFGPYRQ FLDGCFHRN ADRDVLSYG 
      TPTQTKREE PPPTPKGSP DCLPYTCSF NPHKQDVDV 
      FTATISQDN YLNHIWFGF ISLNQQGMK LTDLDGINS 
      DETNSGDNS NAGSSIGPD DDQARLRLK RKLQRNRTS 
      FTNDQIDSL EKEFERTHY PDVFARERL AAKIGLPEA 
      RIQVWFSNR RAKWRREEK LRNQRRGAP APAEHPGPA 
      ASPPRLQGF NNTSMYSPL PPPMSIADT YSSMSSMSS 
      FNHGGLTSS MGPSPGACL QQRESPSMG GAMSGYCMG 
      RPPSYDLGI GYGSRPSPC SPTQPYQMN GHQYNTNGT 
      SSTGLISPG VSVPIAVPG QADMTSQYW PRLQ
    CDS sequence
    • >TC032832 RA
      atgccaacc gccgcccct gcgctcgtg actctttcc 
      tttggtcct tatcgccag tttctggat ggctgcttc 
      caccgtaac gcagatcgg gatgtctta tcttacggg 
      acgcccacc caaactaag cgagaggaa ccaccgccg 
      acccctaag ggctccccc gactgcctc ccctacact 
      tgctcgttt aatccccac aaacaagac gtggatgtg 
      tttaccgcc acgatctca caggataat tacttaaat 
      catatttgg ttcggcttt atctcacta aatcaacag 
      ggaatgaag ttgacggac ctggacggc atcaactcc 
      gacgagacg aattccggt gacaattcc aacgcgggc 
      tcgagcata ggccccgac gacgaccaa gcaagactt 
      aggctaaag aggaagttg caaagaaat cggacgtct 
      ttcacgaac gaccaaata gacagtctc gagaaagaa 
      ttcgaaagg acgcattat ccagatgtc ttcgcgcgt 
      gaaaggtta gcagcaaaa atcggcctt ccggaagca 
      aggattcaa gtgtggttt tcgaatcgg agggctaag 
      tggcgaagg gaggagaaa ttgaggaat cagaggcgg 
      ggggcgcct gcaccggct gaacatcca gggccggcg 
      gcgtcgccc ccaaggcta cagggcttc aacaacacg 
      tccatgtat tcgcccctg cccccgccg atgtccatt 
      gcggacaca tacagctcg atgtcgtcg atgtcgagc 
      ttcaaccac ggagggctg acgtcgagc atgggcccc 
      tctccgggt gcgtgtttg caacaaagg gaaagtccg 
      agtatgggc ggagcaatg agcggttat tgcatggga 
      cgtccacct agttacgac ttggggatc ggatatggg 
      tcaagaccg tcgccttgc agccccact cagccctat 
      cagatgaac ggacaccaa tataacaca aacgggaca 
      tcgtcgaca ggattgata tcgcccgga gtttcggtc 
      ccaattgcg gttccgggt caagctgat atgacgtct 
      caatactgg cctcgttta cagtga
    mRNA sequence
    • >TC032832 RA
      cgcagtttt ttttcctaa tttcaagaa attgaaaat 
      tcatttgaa taattatga accaacttc gtctggaaa 
      tgttttatc gggagtaaa ttagcgccg gtaatgaat 
      cattttatt cgtttgcga attaaataa acataggat 
      ttatgaaat ccttgtcga ggatcgtcc tcgctgcct 
      acacggaat gccaaccgc cgcccctgc gctcgtgac 
      tctttcctt tggtcctta tcgccagtt tctggatgg 
      ctgcttcca ccgtaacgc agatcggga tgtcttatc 
      ttacgggac gcccaccca aactaagcg agaggaacc 
      accgccgac ccctaaggg ctcccccga ctgcctccc 
      ctacacttg ctcgtttaa tccccacaa acaagacgt 
      ggatgtgtt taccgccac gatctcaca ggataatta 
      cttaaatca tatttggtt cggctttat ctcactaaa 
      tcaacaggg aatgaagtt gacggacct ggacggcat 
      caactccga cgagacgaa ttccggtga caattccaa 
      cgcgggctc gagcatagg ccccgacga cgaccaagc 
      aagacttag gctaaagag gaagttgca aagaaatcg 
      gacgtcttt cacgaacga ccaaataga cagtctcga 
      gaaagaatt cgaaaggac gcattatcc agatgtctt 
      cgcgcgtga aaggttagc agcaaaaat cggccttcc 
      ggaagcaag gattcaagt gtggttttc gaatcggag 
      ggctaagtg gcgaaggga ggagaaatt gaggaatca 
      gaggcgggg ggcgcctgc accggctga acatccagg 
      gccggcggc gtcgccccc aaggctaca gggcttcaa 
      caacacgtc catgtattc gcccctgcc cccgccgat 
      gtccattgc ggacacata cagctcgat gtcgtcgat 
      gtcgagctt caaccacgg agggctgac gtcgagcat 
      gggcccctc tccgggtgc gtgtttgca acaaaggga 
      aagtccgag tatgggcgg agcaatgag cggttattg 
      catgggacg tccacctag ttacgactt ggggatcgg 
      atatgggtc aagaccgtc gccttgcag ccccactca 
      gccctatca gatgaacgg acaccaata taacacaaa 
      cgggacatc gtcgacagg attgatatc gcccggagt 
      ttcggtccc aattgcggt tccgggtca agctgatat 
      gacgtctca atactggcc tcgtttaca gtgaccttg 
      gtggaccta aatgatttt ggcgtaaat gttgtctta 
      ccctctttc tagtattgt taaaatttg aagagtttg 
      gaagtgatt gaataccgg gtgtttcca gtccttgag 
      tcgacttat tcattccaa agagttagt gtacaattc 
      atatttttg agcattctt tgtgtaaat gtgatcagg 
      aaattcgtt caacaaata tgtgagata cggggattt 
      attttaatt attttgtgt aacgacata tccaatatc 
      agaataaag cgtttcttt caaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY