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Gene information
General information
TC032832
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008176 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6136608..6140202
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032832
    iBeetle screen iB_07188

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands content opaque - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    abdominal odoriferous glands size increased - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     gena triplet gena triplet dorsal not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032832 RA
      MPTAAPALV TLSFGPYRQ FLDGCFHRN ADRDVLSYG 
TPTQTKREE PPPTPKGSP DCLPYTCSF NPHKQDVDV 
FTATISQDN YLNHIWFGF ISLNQQGMK LTDLDGINS 
DETNSGDNS NAGSSIGPD DDQARLRLK RKLQRNRTS 
FTNDQIDSL EKEFERTHY PDVFARERL AAKIGLPEA 
RIQVWFSNR RAKWRREEK LRNQRRGAP APAEHPGPA 
ASPPRLQGF NNTSMYSPL PPPMSIADT YSSMSSMSS 
FNHGGLTSS MGPSPGACL QQRESPSMG GAMSGYCMG 
RPPSYDLGI GYGSRPSPC SPTQPYQMN GHQYNTNGT 
SSTGLISPG VSVPIAVPG QADMTSQYW PRLQ
    CDS sequence
    • >TC032832 RA
      atgccaacc gccgcccct gcgctcgtg actctttcc 
tttggtcct tatcgccag tttctggat ggctgcttc 
caccgtaac gcagatcgg gatgtctta tcttacggg 
acgcccacc caaactaag cgagaggaa ccaccgccg 
acccctaag ggctccccc gactgcctc ccctacact 
tgctcgttt aatccccac aaacaagac gtggatgtg 
tttaccgcc acgatctca caggataat tacttaaat 
catatttgg ttcggcttt atctcacta aatcaacag 
ggaatgaag ttgacggac ctggacggc atcaactcc 
gacgagacg aattccggt gacaattcc aacgcgggc 
tcgagcata ggccccgac gacgaccaa gcaagactt 
aggctaaag aggaagttg caaagaaat cggacgtct 
ttcacgaac gaccaaata gacagtctc gagaaagaa 
ttcgaaagg acgcattat ccagatgtc ttcgcgcgt 
gaaaggtta gcagcaaaa atcggcctt ccggaagca 
aggattcaa gtgtggttt tcgaatcgg agggctaag 
tggcgaagg gaggagaaa ttgaggaat cagaggcgg 
ggggcgcct gcaccggct gaacatcca gggccggcg 
gcgtcgccc ccaaggcta cagggcttc aacaacacg 
tccatgtat tcgcccctg cccccgccg atgtccatt 
gcggacaca tacagctcg atgtcgtcg atgtcgagc 
ttcaaccac ggagggctg acgtcgagc atgggcccc 
tctccgggt gcgtgtttg caacaaagg gaaagtccg 
agtatgggc ggagcaatg agcggttat tgcatggga 
cgtccacct agttacgac ttggggatc ggatatggg 
tcaagaccg tcgccttgc agccccact cagccctat 
cagatgaac ggacaccaa tataacaca aacgggaca 
tcgtcgaca ggattgata tcgcccgga gtttcggtc 
ccaattgcg gttccgggt caagctgat atgacgtct 
caatactgg cctcgttta cagtga
    mRNA sequence
    • >TC032832 RA
      cgcagtttt ttttcctaa tttcaagaa attgaaaat 
tcatttgaa taattatga accaacttc gtctggaaa 
tgttttatc gggagtaaa ttagcgccg gtaatgaat 
cattttatt cgtttgcga attaaataa acataggat 
ttatgaaat ccttgtcga ggatcgtcc tcgctgcct 
acacggaat gccaaccgc cgcccctgc gctcgtgac 
tctttcctt tggtcctta tcgccagtt tctggatgg 
ctgcttcca ccgtaacgc agatcggga tgtcttatc 
ttacgggac gcccaccca aactaagcg agaggaacc 
accgccgac ccctaaggg ctcccccga ctgcctccc 
ctacacttg ctcgtttaa tccccacaa acaagacgt 
ggatgtgtt taccgccac gatctcaca ggataatta 
cttaaatca tatttggtt cggctttat ctcactaaa 
tcaacaggg aatgaagtt gacggacct ggacggcat 
caactccga cgagacgaa ttccggtga caattccaa 
cgcgggctc gagcatagg ccccgacga cgaccaagc 
aagacttag gctaaagag gaagttgca aagaaatcg 
gacgtcttt cacgaacga ccaaataga cagtctcga 
gaaagaatt cgaaaggac gcattatcc agatgtctt 
cgcgcgtga aaggttagc agcaaaaat cggccttcc 
ggaagcaag gattcaagt gtggttttc gaatcggag 
ggctaagtg gcgaaggga ggagaaatt gaggaatca 
gaggcgggg ggcgcctgc accggctga acatccagg 
gccggcggc gtcgccccc aaggctaca gggcttcaa 
caacacgtc catgtattc gcccctgcc cccgccgat 
gtccattgc ggacacata cagctcgat gtcgtcgat 
gtcgagctt caaccacgg agggctgac gtcgagcat 
gggcccctc tccgggtgc gtgtttgca acaaaggga 
aagtccgag tatgggcgg agcaatgag cggttattg 
catgggacg tccacctag ttacgactt ggggatcgg 
atatgggtc aagaccgtc gccttgcag ccccactca 
gccctatca gatgaacgg acaccaata taacacaaa 
cgggacatc gtcgacagg attgatatc gcccggagt 
ttcggtccc aattgcggt tccgggtca agctgatat 
gacgtctca atactggcc tcgtttaca gtgaccttg 
gtggaccta aatgatttt ggcgtaaat gttgtctta 
ccctctttc tagtattgt taaaatttg aagagtttg 
gaagtgatt gaataccgg gtgtttcca gtccttgag 
tcgacttat tcattccaa agagttagt gtacaattc 
atatttttg agcattctt tgtgtaaat gtgatcagg 
aaattcgtt caacaaata tgtgagata cggggattt 
attttaatt attttgtgt aacgacata tccaatatc 
agaataaag cgtttcttt caaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY