Status
Please wait ...
Gene information
General information
TC008154 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5843998..5854519
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008154
    iBeetle screen iB_07183

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008154 RA
      MTMLTSKAK HYLHCCLFI YVIFMFEIF TGGIKLLDG 
      AFFVPAEDI NPWVHYGYL GALVLYLLR LVTFLPLPQ 
      VLFNFIGLT YYNAFPDKV VLKASPILA PFICIRVVT 
      RGDFPQLVK NNVNRNMNK CLDAGLENF LIEVVTDKK 
      LGMEKHRKV REIVVPQDY RTKSGALFK ARALQYCLE 
      DDVNVLSPN DWIVHLDEE TLLTENSVR GILNFVGDG 
      KHQFGQGLI TYANEEVVN WITTLADSF RVTDDMGKL 
      KLQFRMFHK PLFSWKGSF VVTQVGAER EVSFDNGLD 
      GSIAEDCFF AMKAFSKGY SFNFIEGEM WEKSPFTFW 
      DFIQQRKRW IQGILLVVH SKDIPLRNK LLLACSCYS 
      WLTLPLSVS NLVLASKFP IPCPPIIDF ICAFIGAVN 
      IYMFVFGVI KSFTVYRFG LGRFVLCLC GAVLVIPFN 
      LVIENVAVI WGIFGKKHK FYVVNKNAR PQVTV
    CDS sequence
    • >TC008154 RA
      atgacaatg ctgacgagc aaagcgaag cactacctc 
      cactgctgt ctcttcatc tatgtgata ttcatgttc 
      gagatcttc accggaggg atcaaactc ctcgatggc 
      gcttttttc gtccccgcc gaagacatc aacccctgg 
      gtgcattac gggtacttg ggcgcgttg gtgctctac 
      ctgcttaga ctagtgact ttcctcccc ttaccccaa 
      gtgttgttt aatttcatc ggactgact tactacaac 
      gcgttcccg gataaagtc gttttaaaa gcgtcgccg 
      attttagcg ccgttcatc tgtattagg gtggtgacc 
      aggggcgac ttcccccag ctggtcaag aacaacgtc 
      aaccggaac atgaacaag tgtctagac gccgggctg 
      gagaacttc ctcattgaa gtcgtgact gataaaaaa 
      ctggggatg gagaagcac aggaaggtg agggagatt 
      gtggtcccc caggactac cggaccaag tcgggggcg 
      cttttcaaa gcccgagcg ctgcagtac tgcctggag 
      gacgacgtc aatgtcttg tcgccaaac gactggatc 
      gtgcacctg gacgaagag actctcctg acggagaat 
      tccgtccgg gggattttg aacttcgtc ggggatggc 
      aagcatcag ttcggacaa gggctgatc acttacgcc 
      aacgaagaa gtggttaat tggattacg accttagcg 
      gattctttt agagtcacg gacgatatg ggaaagctc 
      aagttgcaa tttcggatg ttccacaag ccgttgttc 
      agttggaag gggtcgttt gtcgtcacg caggtcggc 
      gcagaacgc gaggtttcc ttcgacaat ggccttgac 
      ggctcgatc gccgaggac tgtttcttc gcgatgaag 
      gccttcagc aaaggctac agtttcaac ttcatcgag 
      ggcgagatg tgggagaag tccccgttc accttctgg 
      gacttcatc cagcagcgg aagcgctgg atccagggc 
      atcctgctg gtggtgcac tccaaggac atcccgctc 
      aggaacaag ctgctcctg gcttgctcg tgctactcc 
      tggctcacg ctaccgctg tccgtctcc aacctggtg 
      ctggcgtcg aagttcccc atcccctgt ccgccgatt 
      attgacttt atttgtgca tttatcggt gctgtgaat 
      atttacatg tttgtgttc ggtgtcata aagtcgttt 
      acggtttac aggttcgga ctgggacgg tttgtgcta 
      tgtttgtgc ggagcggta ctcgttata ccctttaat 
      ttagttatc gaaaacgtc gcggtcatt tgggggatt 
      ttcggcaag aaacataag ttttatgtt gttaataag 
      aacgcgcgg ccgcaggtg acggtctag
    mRNA sequence
    • >TC008154 RA
      ttaaacgaa aaactgcga taaaatttt agattttgg 
      gacaatttg gctgtgttt atgcaatag aattatcag 
      cgctgtatt aaaccttgc cgagctaca cccattaaa 
      tgtggaata ttggtttct gcgatgcgc atgcctccg 
      taagcccca tacagagga atcaacaga aaagccgcg 
      atagcattc tcattgcag atgtctggt gccgaatga 
      tggtgtagt tggacgctc gaacccggc ttttgcaaa 
      tccgtcaca ttttatctg tgatatgta aagtgacgt 
      tccacccct tggtagtac aggccgtcg ggcaccgag 
      tggtgaaaa tcctgcttg cgtgattag tgtccagtg 
      cgagggttg tttgccgaa atgacaatg ctgacgagc 
      aaagcgaag cactacctc cactgctgt ctcttcatc 
      tatgtgata ttcatgttc gagatcttc accggaggg 
      atcaaactc ctcgatggc gcttttttc gtccccgcc 
      gaagacatc aacccctgg gtgcattac gggtacttg 
      ggcgcgttg gtgctctac ctgcttaga ctagtgact 
      ttcctcccc ttaccccaa gtgttgttt aatttcatc 
      ggactgact tactacaac gcgttcccg gataaagtc 
      gttttaaaa gcgtcgccg attttagcg ccgttcatc 
      tgtattagg gtggtgacc aggggcgac ttcccccag 
      ctggtcaag aacaacgtc aaccggaac atgaacaag 
      tgtctagac gccgggctg gagaacttc ctcattgaa 
      gtcgtgact gataaaaaa ctggggatg gagaagcac 
      aggaaggtg agggagatt gtggtcccc caggactac 
      cggaccaag tcgggggcg cttttcaaa gcccgagcg 
      ctgcagtac tgcctggag gacgacgtc aatgtcttg 
      tcgccaaac gactggatc gtgcacctg gacgaagag 
      actctcctg acggagaat tccgtccgg gggattttg 
      aacttcgtc ggggatggc aagcatcag ttcggacaa 
      gggctgatc acttacgcc aacgaagaa gtggttaat 
      tggattacg accttagcg gattctttt agagtcacg 
      gacgatatg ggaaagctc aagttgcaa tttcggatg 
      ttccacaag ccgttgttc agttggaag gggtcgttt 
      gtcgtcacg caggtcggc gcagaacgc gaggtttcc 
      ttcgacaat ggccttgac ggctcgatc gccgaggac 
      tgtttcttc gcgatgaag gccttcagc aaaggctac 
      agtttcaac ttcatcgag ggcgagatg tgggagaag 
      tccccgttc accttctgg gacttcatc cagcagcgg 
      aagcgctgg atccagggc atcctgctg gtggtgcac 
      tccaaggac atcccgctc aggaacaag ctgctcctg 
      gcttgctcg tgctactcc tggctcacg ctaccgctg 
      tccgtctcc aacctggtg ctggcgtcg aagttcccc 
      atcccctgt ccgccgatt attgacttt atttgtgca 
      tttatcggt gctgtgaat atttacatg tttgtgttc 
      ggtgtcata aagtcgttt acggtttac aggttcgga 
      ctgggacgg tttgtgcta tgtttgtgc ggagcggta 
      ctcgttata ccctttaat ttagttatc gaaaacgtc 
      gcggtcatt tgggggatt ttcggcaag aaacataag 
      ttttatgtt gttaataag aacgcgcgg ccgcaggtg 
      acggtctag aggctgaag gtggatgca gaaaatgag 
      aaacgccca atgcggaac tcggacggg ttctcattt 
      tccgcctag tgaaaattt ttactttgt tgtcctttt 
      ataatatac gatgtatta aagatcagg tattttaat 
      acggagcgg tttcacaat tggccacct catccatgt 
      atttattac gagtcattc atttcacta tcaattatg 
      aaatcgccg ggttagtat ttatgcaaa attggaaac 
      agcgcaaac attgttttt ttccatctt attacggac 
      agctttatt aaaacgctg ccttatcaa gatttatac 
      gttccataa actccccca tttcggtgc aaaacaatt 
      accttacta cgggaggac caagtatcg acttatcga 
      aaacttcgg cttttttat gtgttcctg agtcgcgaa 
      atacgtatt ttctttcac cgtttcctg cattcttca 
      ctagcttcc actgtagct tgccattaa ataagcgga 
      ttttgttgt agaaaatca aaacgatta gaatcaaaa 
      gtgtccaga gccaatttt tattgagat taacaagta 
      gtttgttat tgattggtg ttccaaagt aatgaatta 
      gtgtgcgaa gcctctatt ccttgtaca gtaacaaag 
      ttctagcgt tatgttgtt taatttaag acgtaaaca 
      tgtccattt agtaaactg gtctagtgc atgtttata 
      gaacaaaat tttaatact cgccggctc ttcatgtga 
      ctgttaaga ataagtgaa taccccaaa gattatttt 
      ttattgtgt gtaaataac tttgttttt gtgatgaat 
      tcattaaat tttctttca ctgaagaca tactagtca 
      ttattgatt atccaaata gcatcggtt gatcttact 
      tagctgcta gttcgtgta gttcattgt gatttcggg 
      ctatttttt cgagaagca aaaattgtt gttttgaag 
      ccgactgat gatttttta tgtacaaaa cgttaagta 
      ataaaatgc acaatgcca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY