| General information | |||
| TC008154 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5843998..5854519 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008154 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07183 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC008154 RA
MTMLTSKAK HYLHCCLFI YVIFMFEIF TGGIKLLDG AFFVPAEDI NPWVHYGYL GALVLYLLR LVTFLPLPQ VLFNFIGLT YYNAFPDKV VLKASPILA PFICIRVVT RGDFPQLVK NNVNRNMNK CLDAGLENF LIEVVTDKK LGMEKHRKV REIVVPQDY RTKSGALFK ARALQYCLE DDVNVLSPN DWIVHLDEE TLLTENSVR GILNFVGDG KHQFGQGLI TYANEEVVN WITTLADSF RVTDDMGKL KLQFRMFHK PLFSWKGSF VVTQVGAER EVSFDNGLD GSIAEDCFF AMKAFSKGY SFNFIEGEM WEKSPFTFW DFIQQRKRW IQGILLVVH SKDIPLRNK LLLACSCYS WLTLPLSVS NLVLASKFP IPCPPIIDF ICAFIGAVN IYMFVFGVI KSFTVYRFG LGRFVLCLC GAVLVIPFN LVIENVAVI WGIFGKKHK FYVVNKNAR PQVTV
- >TC008154 RA
atgacaatg ctgacgagc aaagcgaag cactacctc cactgctgt ctcttcatc tatgtgata ttcatgttc gagatcttc accggaggg atcaaactc ctcgatggc gcttttttc gtccccgcc gaagacatc aacccctgg gtgcattac gggtacttg ggcgcgttg gtgctctac ctgcttaga ctagtgact ttcctcccc ttaccccaa gtgttgttt aatttcatc ggactgact tactacaac gcgttcccg gataaagtc gttttaaaa gcgtcgccg attttagcg ccgttcatc tgtattagg gtggtgacc aggggcgac ttcccccag ctggtcaag aacaacgtc aaccggaac atgaacaag tgtctagac gccgggctg gagaacttc ctcattgaa gtcgtgact gataaaaaa ctggggatg gagaagcac aggaaggtg agggagatt gtggtcccc caggactac cggaccaag tcgggggcg cttttcaaa gcccgagcg ctgcagtac tgcctggag gacgacgtc aatgtcttg tcgccaaac gactggatc gtgcacctg gacgaagag actctcctg acggagaat tccgtccgg gggattttg aacttcgtc ggggatggc aagcatcag ttcggacaa gggctgatc acttacgcc aacgaagaa gtggttaat tggattacg accttagcg gattctttt agagtcacg gacgatatg ggaaagctc aagttgcaa tttcggatg ttccacaag ccgttgttc agttggaag gggtcgttt gtcgtcacg caggtcggc gcagaacgc gaggtttcc ttcgacaat ggccttgac ggctcgatc gccgaggac tgtttcttc gcgatgaag gccttcagc aaaggctac agtttcaac ttcatcgag ggcgagatg tgggagaag tccccgttc accttctgg gacttcatc cagcagcgg aagcgctgg atccagggc atcctgctg gtggtgcac tccaaggac atcccgctc aggaacaag ctgctcctg gcttgctcg tgctactcc tggctcacg ctaccgctg tccgtctcc aacctggtg ctggcgtcg aagttcccc atcccctgt ccgccgatt attgacttt atttgtgca tttatcggt gctgtgaat atttacatg tttgtgttc ggtgtcata aagtcgttt acggtttac aggttcgga ctgggacgg tttgtgcta tgtttgtgc ggagcggta ctcgttata ccctttaat ttagttatc gaaaacgtc gcggtcatt tgggggatt ttcggcaag aaacataag ttttatgtt gttaataag aacgcgcgg ccgcaggtg acggtctag
- >TC008154 RA
ttaaacgaa aaactgcga taaaatttt agattttgg gacaatttg gctgtgttt atgcaatag aattatcag cgctgtatt aaaccttgc cgagctaca cccattaaa tgtggaata ttggtttct gcgatgcgc atgcctccg taagcccca tacagagga atcaacaga aaagccgcg atagcattc tcattgcag atgtctggt gccgaatga tggtgtagt tggacgctc gaacccggc ttttgcaaa tccgtcaca ttttatctg tgatatgta aagtgacgt tccacccct tggtagtac aggccgtcg ggcaccgag tggtgaaaa tcctgcttg cgtgattag tgtccagtg cgagggttg tttgccgaa atgacaatg ctgacgagc aaagcgaag cactacctc cactgctgt ctcttcatc tatgtgata ttcatgttc gagatcttc accggaggg atcaaactc ctcgatggc gcttttttc gtccccgcc gaagacatc aacccctgg gtgcattac gggtacttg ggcgcgttg gtgctctac ctgcttaga ctagtgact ttcctcccc ttaccccaa gtgttgttt aatttcatc ggactgact tactacaac gcgttcccg gataaagtc gttttaaaa gcgtcgccg attttagcg ccgttcatc tgtattagg gtggtgacc aggggcgac ttcccccag ctggtcaag aacaacgtc aaccggaac atgaacaag tgtctagac gccgggctg gagaacttc ctcattgaa gtcgtgact gataaaaaa ctggggatg gagaagcac aggaaggtg agggagatt gtggtcccc caggactac cggaccaag tcgggggcg cttttcaaa gcccgagcg ctgcagtac tgcctggag gacgacgtc aatgtcttg tcgccaaac gactggatc gtgcacctg gacgaagag actctcctg acggagaat tccgtccgg gggattttg aacttcgtc ggggatggc aagcatcag ttcggacaa gggctgatc acttacgcc aacgaagaa gtggttaat tggattacg accttagcg gattctttt agagtcacg gacgatatg ggaaagctc aagttgcaa tttcggatg ttccacaag ccgttgttc agttggaag gggtcgttt gtcgtcacg caggtcggc gcagaacgc gaggtttcc ttcgacaat ggccttgac ggctcgatc gccgaggac tgtttcttc gcgatgaag gccttcagc aaaggctac agtttcaac ttcatcgag ggcgagatg tgggagaag tccccgttc accttctgg gacttcatc cagcagcgg aagcgctgg atccagggc atcctgctg gtggtgcac tccaaggac atcccgctc aggaacaag ctgctcctg gcttgctcg tgctactcc tggctcacg ctaccgctg tccgtctcc aacctggtg ctggcgtcg aagttcccc atcccctgt ccgccgatt attgacttt atttgtgca tttatcggt gctgtgaat atttacatg tttgtgttc ggtgtcata aagtcgttt acggtttac aggttcgga ctgggacgg tttgtgcta tgtttgtgc ggagcggta ctcgttata ccctttaat ttagttatc gaaaacgtc gcggtcatt tgggggatt ttcggcaag aaacataag ttttatgtt gttaataag aacgcgcgg ccgcaggtg acggtctag aggctgaag gtggatgca gaaaatgag aaacgccca atgcggaac tcggacggg ttctcattt tccgcctag tgaaaattt ttactttgt tgtcctttt ataatatac gatgtatta aagatcagg tattttaat acggagcgg tttcacaat tggccacct catccatgt atttattac gagtcattc atttcacta tcaattatg aaatcgccg ggttagtat ttatgcaaa attggaaac agcgcaaac attgttttt ttccatctt attacggac agctttatt aaaacgctg ccttatcaa gatttatac gttccataa actccccca tttcggtgc aaaacaatt accttacta cgggaggac caagtatcg acttatcga aaacttcgg cttttttat gtgttcctg agtcgcgaa atacgtatt ttctttcac cgtttcctg cattcttca ctagcttcc actgtagct tgccattaa ataagcgga ttttgttgt agaaaatca aaacgatta gaatcaaaa gtgtccaga gccaatttt tattgagat taacaagta gtttgttat tgattggtg ttccaaagt aatgaatta gtgtgcgaa gcctctatt ccttgtaca gtaacaaag ttctagcgt tatgttgtt taatttaag acgtaaaca tgtccattt agtaaactg gtctagtgc atgtttata gaacaaaat tttaatact cgccggctc ttcatgtga ctgttaaga ataagtgaa taccccaaa gattatttt ttattgtgt gtaaataac tttgttttt gtgatgaat tcattaaat tttctttca ctgaagaca tactagtca ttattgatt atccaaata gcatcggtt gatcttact tagctgcta gttcgtgta gttcattgt gatttcggg ctatttttt cgagaagca aaaattgtt gttttgaag ccgactgat gatttttta tgtacaaaa cgttaagta ataaaatgc acaatgcca a
