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Gene information
General information
TC008147 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5795988..5800127
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008147
    iBeetle screen iB_07181

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008147 RA
      MDQTRPEPN TESQTIAPS EITIDNSHI 
      STPTVPSIV FVPTSSSSD DIPPPKADF 
      YAPPPYEVA TKTTKLPTY EEVQREKNL 
      EEQNVPINT LPPRLSQYR APGQRILTI 
      ENESNEDVD TSLLGTDFM FYIAFFVAF 
      IFNWIGFLL LMCFCHTIA SRYGALSGF 
      GLSLAKWTF IVQHSTDLA SKDNSWLWW 
      LIMTFGLII CIRAILQYL SIKRGWHML 
      SASHQERLL FFY
    CDS sequence
    • >TC008147 RA
      atggaccaa accagaccc gagccaaat accgaaagc 
      cagacgatt gctccgtcg gaaataacg attgataat 
      agccacatt tcgactcca acggtcccc agcattgtt 
      tttgtcccc acaagttct tcaagtgac gatattcca 
      ccccccaaa gctgatttt tacgcacca cccccttac 
      gaagttgcc accaaaacc actaaatta cccacgtat 
      gaagaggta caaagggag aaaaatctc gaggagcaa 
      aatgttcca ataaacacg ttaccgcca agactctca 
      cagtatagg gcgccagga cagaggatt ctcacaatt 
      gagaatgaa tcaaacgaa gacgtggac accagttta 
      ttgggaacg gacttcatg ttctacatc gcttttttt 
      gttgcgttc attttcaac tggattggg tttttactg 
      ttgatgtgc ttttgccac acgattgcg tcgaggtac 
      ggcgccttg tcgggattc ggtttgtcc ctcgcaaag 
      tggacattt attgtccag cattcaaca gacctagcg 
      tcaaaagac aacagctgg ctctggtgg ttaataatg 
      acctttggg ttaattatt tgcatacgt gcgatcctt 
      cagtatttg agcataaag agggggtgg cacatgctg 
      tcggcaagc caccaggag agactttta tttttttat 
      taa
    mRNA sequence
    • >TC008147 RA
      tgagggtgc tgtctacga tttatggct tccggtatt 
      tcaaaggaa taatccgct taatacatt caaaagtac 
      cttgacaag tccggcgag acgtcttga gctgataaa 
      tccgtaaca aatcgctat tttccaaca attaagaac 
      acaacaaac aagtttcaa acaatttgg caggtttga 
      ttttcgaaa agtgggcct tgaattcgt tgacacttt 
      gaaaaaaca gtcacaaaa ctacgtttc cggtcggtt 
      taaatgagg aaaagtccg gttttttgc aacttttgt 
      acttttgag ttaaataaa tataccacg cgttggtac 
      aacataaca cattcaccc accaaagca aaactgtca 
      actgttgcg ttttacgct acaaaaaat aaaacgcga 
      ctggagcgt atggtggca gcattgcca actaaggac 
      tgccaacag gaaactgca gcggagcca aattcccaa 
      ttgttatca aattgaact gacattttt aaccataac 
      taatcataa cagctgata agaggttgg aaacatatt 
      ttcaaagtt gtaataagg gaaatacaa aactcgact 
      cgacttaca caatgcgtg cgcacatga ttactcata 
      caggcgcaa cgttgcgct gttgacatt taagtgtaa 
      taaaatggt tgccgtttc ataaagcgt tgcgtttaa 
      aaaattact gtgcgtgca ctgcatttc cacttttga 
      ctgatatat gtcccaaga taagatgga ccaaaccag 
      acccgagcc aaataccga aagccagac gattgctcc 
      gtcggaaat aacgattga taatagcca catttcgac 
      tccaacggt ccccagcat tgtttttgt ccccacaag 
      ttcttcaag tgacgatat tccaccccc caaagctga 
      tttttacgc accaccccc ttacgaagt tgccaccaa 
      aaccactaa attacccac gtatgaaga ggtacaaag 
      ggagaaaaa tctcgagga gcaaaatgt tccaataaa 
      cacgttacc gccaagact ctcacagta tagggcgcc 
      aggacagag gattctcac aattgagaa tgaatcaaa 
      cgaagacgt ggacaccag tttattggg aacggactt 
      catgttcta catcgcttt ttttgttgc gttcatttt 
      caactggat tgggttttt actgttgat gtgcttttg 
      ccacacgat tgcgtcgag gtacggcgc cttgtcggg 
      attcggttt gtccctcgc aaagtggac atttattgt 
      ccagcattc aacagacct agcgtcaaa agacaacag 
      ctggctctg gtggttaat aatgacctt tgggttaat 
      tatttgcat acgtgcgat ccttcagta tttgagcat 
      aaagagggg gtggcacat gctgtcggc aagccacca 
      ggagagact tttattttt ttattaagc cctagaaat 
      caaatattt ataaatgtg attaataaa atgcttaga 
      tattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY