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Gene information
General information
TC008146 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5749805..5753741
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008146
    iBeetle screen iB_07180

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008146 RA
      MENNSRLNG AGRILIFIL VSFPVFGTS HTRHGDGTI 
      LRASVGCSQ PNSTFLICR NRQLRGLQT ILNVARVSF 
      ENVTGEHLD AKNIEHLRW TNSGIVDIK KAIVNPQSL 
      ITLDLTSNN VTKLQDYYF QQYTNLKEM NLSYNAIDD 
      LPRYVFVNQ SLRILALAH NSLQAIPFQ VFAPMQRLQ 
      ILDISYNHI VAILDHFFK FNKHIELLA LNNNKIAKL 
      TSNALADLS DLRTLDLSH NSIKSISKG LFDSLKNLK 
      YLNLANNPL TNVPSGTFR GLTNLRELN LSGNKLMHF 
      TFGLLHFSP ELTSLTLDD THIEELHNS ELLGIAKLE 
      TLRIRNNKQ LREIENYVF ADTPRLKEL DISGNALTF 
      LPISLANLT TLKRLNISD NPWACDCRM YWFAPWAEE 
      KRHHDLILS DLSCGPYAY PNDMLPTLN HLNCTGPRL 
      NYKTPTKQY RLKSDALLE CRYTANPPP SITWVTPSR 
      EVFHWNPDP AIPDIFEKH AHAHDALMI PIRVIPPRI 
      QVLDNGTLL IKNVTRKDC GRYTCYASN PIANLTENV 
      LLHIDPTDW NNVRIMSLI VGLQCATAF LALTLFIQL 
      IRYLIRRFG IFNNFCSFC KRDRVSPRA RQIYAMLDN 
      IEQYKSQQL ERLRDNYTQ QVNRIKENC AQQMEWIQN 
      SYQTQTKHL KDFRDIGSQ HLSTLRGQY CDQVKKVRD 
      YSTTQLNWV RENYVFQRN KIRKFSAHK VLQLRETYK 
      YQQQTLNKV LENLPSLYF ENCRAGTCG RAESLVFDP 
      NDVESIDVY LKAKIEKLA NLEEVTNTD ITQSRQSLY 
      YTPTERSLG SRILSPGDP PIEVFINHI TSRPMGLPP 
      FVDGPSTSH ETITSNGTN RSSLKTSHS TEELLAKNS 
      SCDIQLSTS LPELSVGDV VEDASKRHE TAL
    CDS sequence
    • >TC008146 RA
      atggaaaat aacagccgg ctaaatggt gccggtcgg 
      atcttgatc ttcatatta gtgtcgttt cctgttttc 
      ggaacgagt catacgcgt cacggtgac ggtactatt 
      ttacgcgct tctgtagga tgttctcaa ccgaattcg 
      acgttctta atatgtcgc aacaggcag ttgagaggt 
      cttcaaacg attttgaac gtggcccgt gtcagtttc 
      gaaaatgtc accggcgaa catctagac gcaaaaaac 
      atagaacac ctccgatgg actaacagc ggaatcgtc 
      gatatcaaa aaagcaatc gtcaatcca cagtcgcta 
      atcactcta gacctaacc agcaacaat gtaaccaaa 
      ctacaagac tactatttc cagcagtat actaactta 
      aaagaaatg aatttgtcg tataacgca atcgacgat 
      cttcccaga tacgttttt gtcaatcag tctttgaga 
      atcctagca ctggcacac aactcgtta caagcaata 
      cctttccaa gtattcgcc cccatgcaa cgactccaa 
      attctggac atctcgtac aaccacatc gtggccatc 
      ctagaccac ttcttcaaa ttcaacaaa cacatcgaa 
      ttgttagcg ctaaacaac aacaaaatc gccaaattg 
      acgtcgaac gctttggct gatctatcc gatctcaga 
      accttggac ctatcccac aattcgatt aaatccatc 
      tcgaaagga ctcttcgat tcacttaaa aacctaaag 
      tacttaaat ctcgccaac aaccctctt acgaacgtc 
      ccgagtggt actttccgc ggcttgacg aacctccgc 
      gaattaaac ctgagcggc aacaaactc atgcatttc 
      accttcgga ctgctacac ttcagtcct gaactaaca 
      tctctcaca cttgacgac acccacatt gaagagctc 
      cacaacagc gaactcctc ggcatcgca aaacttgaa 
      accctccgc atccgaaac aacaaacaa ttgcgggaa 
      attgaaaac tacgtcttc gcggacact cctcgcctc 
      aaggagctc gacatcagc gggaacgct ctcactttc 
      ttgcccata tctcttgcc aacctaacc acgctgaaa 
      cgcctcaac atttcggac aacccgtgg gcttgcgac 
      tgtcgaatg tattggttt gcgccgtgg gccgaggag 
      aagcgacac cacgacctg atcttatcc gatttgagc 
      tgcggaccg tacgcctac cccaacgac atgcttccg 
      actttaaac catctcaac tgcaccggc ccgcgtctc 
      aattacaaa acaccaacc aagcagtat cgactcaaa 
      tcggacgct ttgctggag tgtcgttac accgccaac 
      ccgccgcct tccatcacc tgggtcacg cccagcagg 
      gaggtgttc cactggaac ccggatccc gccattccc 
      gacatcttc gagaagcat gcccacgct cacgacgcg 
      ctcatgatc ccgattcgg gtgattcct cctcggatt 
      caagtgctg gacaacggg acgcttctg atcaaaaac 
      gtgacgcgg aaggactgc ggccggtac acgtgttac 
      gcgagcaat cccattgct aacttaacg gaaaacgtg 
      ctccttcat atagatccc accgactgg aataacgtc 
      cggattatg agtcttatc gtgggattg caatgtgcg 
      acggctttc ctcgctctc acactcttc atccaactt 
      atacgctac ttgattaga aggtttggt attttcaac 
      aatttttgc agcttttgc aagcgcgat cgcgtgtca 
      ccacgggca cgccaaatc tacgccatg ctcgacaac 
      atcgagcaa tacaagtcc caacaactg gagcgactt 
      cgggacaat tacactcag caggtcaac aggatcaaa 
      gagaactgc gcccagcag atggaatgg attcaaaat 
      agttatcag actcagact aaacatttg aaagatttt 
      cgcgacatc ggctcccag cacttgtcg actctccga 
      ggccagtat tgcgaccag gttaaaaaa gtcagagac 
      tactcgacc acgcaactc aactgggtt agagaaaac 
      tatgttttc cagaggaac aagattcgg aaattcagt 
      gcacataag gtacttcag ttgagggaa acctacaag 
      taccaacag caaaccctg aacaaagtc ttggagaat 
      cttccgagt ttgtatttc gagaattgt cgcgctggt 
      acttgcgga agggcggaa tctctcgtc ttcgacccg 
      aacgacgtt gaaagcatc gacgtgtat ttgaaagcc 
      aagattgaa aaactcgcc aatttggag gaggtgacc 
      aacacggat attacgcaa agccgccaa tcgctatat 
      tacacgcct acggaacga tccttaggc tcgaggata 
      ctttcgccg ggagatccg ccgattgaa gtcttcatc 
      aaccatata acaagcaga cccatggga ttaccaccg 
      ttcgtcgat ggaccatcg acttcacac gaaactatt 
      acgtccaat ggcactaat aggagtagt ctcaagacg 
      tcgcatagt accgaagaa ctgttggcg aagaacagt 
      tcttgtgat atacagttg agcacgagt ctaccagaa 
      ttgtcggtc ggggacgtc gttgaagat gcaagcaaa 
      cggcatgaa acggcttta tag
    mRNA sequence
    • >TC008146 RA
      gctctgtcc tgtacagtt cagtgttgc attcttcca 
      caatcgtga aaaatcgta ccattatac tgtaaatcc 
      ttgtgtcct tttacatta aatgcttgt ttcaacaca 
      aataaagta aaaataaaa cgtggcttt atcatatgg 
      attgaaagg aaggaagga agaggtcaa gatcatata 
      ggacaatgt attgtgaat tatggaaaa taacagccg 
      gctaaatgg tgccggtcg gatcttgat cttcatatt 
      agtgtcgtt tcctgtttt cggaacgag tcatacgcg 
      tcacggtga cggtactat tttacgcgc ttctgtagg 
      atgttctca accgaattc gacgttctt aatatgtcg 
      caacaggca gttgagagg tcttcaaac gattttgaa 
      cgtggcccg tgtcagttt cgaaaatgt caccggcga 
      acatctaga cgcaaaaaa catagaaca cctccgatg 
      gactaacag cggaatcgt cgatatcaa aaaagcaat 
      cgtcaatcc acagtcgct aatcactct agacctaac 
      cagcaacaa tgtaaccaa actacaaga ctactattt 
      ccagcagta tactaactt aaaagaaat gaatttgtc 
      gtataacgc aatcgacga tcttcccag atacgtttt 
      tgtcaatca gtctttgag aatcctagc actggcaca 
      caactcgtt acaagcaat acctttcca agtattcgc 
      ccccatgca acgactcca aattctgga catctcgta 
      caaccacat cgtggccat cctagacca cttcttcaa 
      attcaacaa acacatcga attgttagc gctaaacaa 
      caacaaaat cgccaaatt gacgtcgaa cgctttggc 
      tgatctatc cgatctcag aaccttgga cctatccca 
      caattcgat taaatccat ctcgaaagg actcttcga 
      ttcacttaa aaacctaaa gtacttaaa tctcgccaa 
      caaccctct tacgaacgt cccgagtgg tactttccg 
      cggcttgac gaacctccg cgaattaaa cctgagcgg 
      caacaaact catgcattt caccttcgg actgctaca 
      cttcagtcc tgaactaac atctctcac acttgacga 
      cacccacat tgaagagct ccacaacag cgaactcct 
      cggcatcgc aaaacttga aaccctccg catccgaaa 
      caacaaaca attgcggga aattgaaaa ctacgtctt 
      cgcggacac tcctcgcct caaggagct cgacatcag 
      cgggaacgc tctcacttt cttgcccat atctcttgc 
      caacctaac cacgctgaa acgcctcaa catttcgga 
      caacccgtg ggcttgcga ctgtcgaat gtattggtt 
      tgcgccgtg ggccgagga gaagcgaca ccacgacct 
      gatcttatc cgatttgag ctgcggacc gtacgccta 
      ccccaacga catgcttcc gactttaaa ccatctcaa 
      ctgcaccgg cccgcgtct caattacaa aacaccaac 
      caagcagta tcgactcaa atcggacgc tttgctgga 
      gtgtcgtta caccgccaa cccgccgcc ttccatcac 
      ctgggtcac gcccagcag ggaggtgtt ccactggaa 
      cccggatcc cgccattcc cgacatctt cgagaagca 
      tgcccacgc tcacgacgc gctcatgat cccgattcg 
      ggtgattcc tcctcggat tcaagtgct ggacaacgg 
      gacgcttct gatcaaaaa cgtgacgcg gaaggactg 
      cggccggta cacgtgtta cgcgagcaa tcccattgc 
      taacttaac ggaaaacgt gctccttca tatagatcc 
      caccgactg gaataacgt ccggattat gagtcttat 
      cgtgggatt gcaatgtgc gacggcttt cctcgctct 
      cacactctt catccaact tatacgcta cttgattag 
      aaggtttgg tattttcaa caatttttg cagcttttg 
      caagcgcga tcgcgtgtc accacgggc acgccaaat 
      ctacgccat gctcgacaa catcgagca atacaagtc 
      ccaacaact ggagcgact tcgggacaa ttacactca 
      gcaggtcaa caggatcaa agagaactg cgcccagca 
      gatggaatg gattcaaaa tagttatca gactcagac 
      taaacattt gaaagattt tcgcgacat cggctccca 
      gcacttgtc gactctccg aggccagta ttgcgacca 
      ggttaaaaa agtcagaga ctactcgac cacgcaact 
      caactgggt tagagaaaa ctatgtttt ccagaggaa 
      caagattcg gaaattcag tgcacataa ggtacttca 
      gttgaggga aacctacaa gtaccaaca gcaaaccct 
      gaacaaagt cttggagaa tcttccgag tttgtattt 
      cgagaattg tcgcgctgg tacttgcgg aagggcgga 
      atctctcgt cttcgaccc gaacgacgt tgaaagcat 
      cgacgtgta tttgaaagc caagattga aaaactcgc 
      caatttgga ggaggtgac caacacgga tattacgca 
      aagccgcca atcgctata ttacacgcc tacggaacg 
      atccttagg ctcgaggat actttcgcc gggagatcc 
      gccgattga agtcttcat caaccatat aacaagcag 
      acccatggg attaccacc gttcgtcga tggaccatc 
      gacttcaca cgaaactat tacgtccaa tggcactaa 
      taggagtag tctcaagac gtcgcatag taccgaaga 
      actgttggc gaagaacag ttcttgtga tatacagtt 
      gagcacgag tctaccaga attgtcggt cggggacgt 
      cgttgaaga tgcaagcaa acggcatga aacggcttt 
      atagactcg ccgcctagt ttaatcaac tgctgtgat 
      aaagtgagt acgctatca cagtgtaga gtccgctcc 
      attagtgcc aatctatag gaagctgtc atttttgtt 
      ttttgtttg tcgtggcca aaacaatga acgaaattt 
      tttttcttt gtacaattg tttaaatgg gaatatact 
      agactcagt ataccgaag aactagcta gtcaagaat 
      aaaaaaaaa gactgacga ttcttcaag gaaaaagtc 
      gcgccgcgt tcttgtttc tgggaaatg atttgccgc 
      ttcacgaat aacctttct tttttcaat tgataagtt 
      tagggaata ttttaacga aggtaaaat taggtttta 
      ctatagcgc catgtacat ttttaattt tttagctgt 
      gaatctgta attaagaat catctcatt atgtaatgt 
      tttgatagt gtaactata gaaagttac tatttatat 
      acctagata aacgacatt cggtgtttt gagtcctag 
      acattgttt atccacatc tactgctga tgatttcta 
      cactggtat agggaacgt ttttttata agcgggtac 
      caactaaga tacaactat ttttattat cgaaatgtt 
      attgtgatt tcattaagt gttaaaatt agcatgtat 
      ttgctatct attaataaa atattctaa gaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY