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Gene information
General information
TC008078 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4753092..4755203
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008078
    iBeetle screen iB_07173

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008078 RA
      MDDEDNTCR DGDDDSDEW DEMEVTGEQ TTCLFCPLQ 
      FLTIAVALD HCRAEHNFD LLELKNKYN MDCYSYIKM 
      INYIRMQRP DPKILTESS VALWDDDVY LKAGEMESW 
      LMYDFEDLG SAPSTPHYA IDGKTPISN LNFSDLQRQ 
      IEDLTLQLK HKCTLLELA VKDIEKMKK ITRTFVESR 
      DVPEKPFHV TGYDSDYFH SYSHFGIHH EMLNDRVRT 
      ESYRDAILN NSDSFKDKI VLDVGCGTG ILSLFSAKA 
      GASKVIGID QSEVVYKAM DIIRENNYY DTIHLMKGR 
      IEDTNLPVE KVDIIVSEW MGYFLLFEG MLDSFIHAR 
      DRYLAPGGL LLPNRCNLN LIGCSDPER YDKVINFWD 
      NVYGFSMKC MKSEVISEA FVETVPGES VMTDPITLK 
      EIDLASCTV DTCDFSSAF ALKATKDAV LTCLVGYFD 
      TFFDLPKSV HFSTGPEAP KTHWQQSVF YLKETINMS 
      AGDVIEGSL VCSRLKKNA RGLSVTITL GKNKYHYNL 
      D
    CDS sequence
    • >TC008078 RA
      atggatgat gaggacaac acgtgtagg gacggagat 
      gacgattct gacgaatgg gacgaaatg gaagtcaca 
      ggggagcaa acgacgtgt cttttttgc cccctgcaa 
      ttcctgaca atagcggtg gcgctggac cattgtcgt 
      gctgagcac aactttgat ttattagag ttgaaaaat 
      aagtataac atggactgt tattcctac atcaagatg 
      attaattac attaggatg caaagaccg gacccgaaa 
      atcttgacc gagtcttcg gtcgcgttg tgggacgat 
      gatgtgtat ttgaaggcg ggcgagatg gagtcatgg 
      ttgatgtat gactttgag gacctggga agtgccccc 
      tccactccc cattatgcc atcgacggc aagacgccc 
      atcagcaat ttgaatttt tccgacctg cagaggcaa 
      attgaggac ttgacttta caattgaaa cacaaatgc 
      acgttacta gaactcgcg gtgaaagac atcgaaaag 
      atgaaaaaa atcactagg acgtttgtt gaaagtagg 
      gatgttcca gaaaagccg tttcacgtc actggttat 
      gactctgat tatttccac tcgtattca catttcggt 
      attcaccac gaaatgtta aatgataga gtcaggact 
      gaaagctac agggacgca attttaaat aattcggac 
      agtttcaag gacaaaatc gtcctggac gtgggctgt 
      ggcactggg attttgtcg ctgttttcg gccaaagct 
      ggcgcttcc aaagtgatt ggaatcgac cagtctgag 
      gtcgtttac aaagcaatg gacataatc agagaaaac 
      aattattac gacacaatc cacttgatg aaaggccga 
      atcgaggac actaatctc ccggttgaa aaagtcgac 
      ataatcgtc tcggaatgg atgggctat tttttgctg 
      ttcgaaggc atgttggac agtttcata cacgcgaga 
      gatcgctac ttggcccca ggaggcctc cttttgcca 
      aacaggtgc aacttgaac ctgattggc tgctctgac 
      cccgagcgt tacgacaaa gtgattaat ttctgggac 
      aatgtttat gggttttcg atgaaatgc atgaagagt 
      gaagtcatt tccgaggct tttgtcgaa accgttcct 
      ggcgaaagt gtcatgact gaccccata acactgaag 
      gagattgat ttggcaagt tgtaccgtt gatacttgc 
      gattttagc tcggctttt gcgttgaaa gccaccaaa 
      gatgccgtt ttgacatgc ttggtgggg tattttgat 
      acgttcttt gacttacca aaaagtgtt cacttttcg 
      acggggcct gaagcgccc aaaacgcac tggcaacaa 
      agtgttttt tatttgaag gagacaata aatatgagt 
      gcaggtgat gtgattgaa gggtcgcta gtttgttca 
      cggctcaaa aagaatgcg agaggtttg tctgtgacc 
      ataacttta ggtaaaaat aaatatcac tataatttg 
      gactag
    mRNA sequence
    • >TC008078 RA
      atcaataat ttcaattta caatcatta aaattcaag 
      ttttaagct ttaatttcg tatagaggt gccatagcg 
      ccccaaatt gtgccaaaa cttgggttg aattactct 
      tacgggaaa tttaacctc aatctgttg ccgtacacg 
      tgtttgtgt ttataattc gcccctgag ggttttaac 
      aaagaaaca atggatgat gaggacaac acgtgtagg 
      gacggagat gacgattct gacgaatgg gacgaaatg 
      gaagtcaca ggggagcaa acgacgtgt cttttttgc 
      cccctgcaa ttcctgaca atagcggtg gcgctggac 
      cattgtcgt gctgagcac aactttgat ttattagag 
      ttgaaaaat aagtataac atggactgt tattcctac 
      atcaagatg attaattac attaggatg caaagaccg 
      gacccgaaa atcttgacc gagtcttcg gtcgcgttg 
      tgggacgat gatgtgtat ttgaaggcg ggcgagatg 
      gagtcatgg ttgatgtat gactttgag gacctggga 
      agtgccccc tccactccc cattatgcc atcgacggc 
      aagacgccc atcagcaat ttgaatttt tccgacctg 
      cagaggcaa attgaggac ttgacttta caattgaaa 
      cacaaatgc acgttacta gaactcgcg gtgaaagac 
      atcgaaaag atgaaaaaa atcactagg acgtttgtt 
      gaaagtagg gatgttcca gaaaagccg tttcacgtc 
      actggttat gactctgat tatttccac tcgtattca 
      catttcggt attcaccac gaaatgtta aatgataga 
      gtcaggact gaaagctac agggacgca attttaaat 
      aattcggac agtttcaag gacaaaatc gtcctggac 
      gtgggctgt ggcactggg attttgtcg ctgttttcg 
      gccaaagct ggcgcttcc aaagtgatt ggaatcgac 
      cagtctgag gtcgtttac aaagcaatg gacataatc 
      agagaaaac aattattac gacacaatc cacttgatg 
      aaaggccga atcgaggac actaatctc ccggttgaa 
      aaagtcgac ataatcgtc tcggaatgg atgggctat 
      tttttgctg ttcgaaggc atgttggac agtttcata 
      cacgcgaga gatcgctac ttggcccca ggaggcctc 
      cttttgcca aacaggtgc aacttgaac ctgattggc 
      tgctctgac cccgagcgt tacgacaaa gtgattaat 
      ttctgggac aatgtttat gggttttcg atgaaatgc 
      atgaagagt gaagtcatt tccgaggct tttgtcgaa 
      accgttcct ggcgaaagt gtcatgact gaccccata 
      acactgaag gagattgat ttggcaagt tgtaccgtt 
      gatacttgc gattttagc tcggctttt gcgttgaaa 
      gccaccaaa gatgccgtt ttgacatgc ttggtgggg 
      tattttgat acgttcttt gacttacca aaaagtgtt 
      cacttttcg acggggcct gaagcgccc aaaacgcac 
      tggcaacaa agtgttttt tatttgaag gagacaata 
      aatatgagt gcaggtgat gtgattgaa gggtcgcta 
      gtttgttca cggctcaaa aagaatgcg agaggtttg 
      tctgtgacc ataacttta ggtaaaaat aaatatcac 
      tataatttg gactagtgg tttgtaatt tttattttt 
      caagtaaat aaaataaaa aaagtgttt acgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY