Status
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Gene information
General information
TC008070 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4522902..4524948
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008070
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07172

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008070 RA
      MAAVILLLV TAMALAAPD WTDCPSPCR CKWSSGKKT 
      AVCKGGGFS AVPDTLDGE MQVLDLSGN YISRLGNDA 
      FKSVGLLNL QRIFLATAG IQEVHKDAF RDLTILVEV 
      DLSHNQIKS LHPETFHGN ERLRVLYLN GNPLRRLVQ 
      EQFPQLPHL RILELDGCQ LEYVHKNAF VHLSVLETL 
      SLRQNLLRN LSEEVFMNF AHLKTLVLE GNPWKCDCE 
      LRGFRDWFL LSKLNSVSL TCTQPERLE GKLWENVLS 
      EEFACPPRV SVFPQSQVQ AEAGGNVSF GCHVTGDPE 
      PQVSWLYEG YPINHTWLV IQAEEGLLD KWANISVYN 
      VSDTDTGVY TCTARNILD SVSLNVTLV LPEVVTATT 
      VSKSEGGFL WWGLLVALV TLGSVGLGA SIAVCCLRG 
      RKTQKRMKA SVSFTEQEK KLLDVSIAT TTDRGTGSC 
      EVLGPEMEL DPPVHITIE SEPLPLAVF PPPPEFGNV 
      VPSAYGNIF ISVSVSRDP SDASRCPDL LDLPHRAKP 
      VYHGMATLP RRPCAAPHY DNMGPRVTA AGSSTLSLP 
      GVPPPPPPP PPPQLTPEF VSL
    CDS sequence
    • >TC008070 RA
      atggcggcg gtgatcctt ctcctggtg acggccatg 
      gccctggcg gccccggac tggaccgac tgtccgtcg 
      ccgtgccgg tgcaagtgg tcgtcgggc aaaaagacg 
      gcggtatgt aaggggggc ggcttctcc gccgtcccc 
      gacacgctc gatggcgag atgcaggtg ctggacctg 
      tcggggaac tacatctct cggctgggg aacgatgcg 
      ttcaaaagt gtcgggctg ctgaacctg cagcggatt 
      ttcctggcc acggccggg atccaggag gtccacaag 
      gacgccttc cgggatttg actattttg gtcgaggtc 
      gacttgtcg cataatcag atcaagagt ttgcatccg 
      gagacgttt cacgggaat gagcggcta cgcgtgttg 
      tatctcaat gggaatccg ctgaggagg ctggtccag 
      gagcagttt ccccagctg ccgcatctc cggattttg 
      gagcttgat ggctgtcag ctggaatat gttcacaag 
      aacgctttc gtgcatttg agtgtgttg gagactctg 
      agtctgaga cagaattta ttgcggaat ctttcggag 
      gaggttttt atgaatttt gctcatttg aagactttg 
      gttttggaa gggaatcct tggaagtgc gattgtgag 
      ttgcgcggg tttagggat tggtttctc ttgagtaaa 
      ttgaattcg gtttctttg acttgtact cagcctgaa 
      cggcttgaa ggcaagttg tgggaaaac gttctttcg 
      gaagaattc gcttgtccg cctcgcgta tcagttttc 
      ccccaaagt caagtccag gctgaagct ggcggaaat 
      gtcagtttt gggtgtcac gtgactggg gacccggaa 
      ccccaagtc tcctggctt tatgaaggt tatccgata 
      aatcatacg tggttagtg attcaagcc gaagaagga 
      cttttagat aagtgggcc aatataagt gtttacaac 
      gtcagtgat acagacaca ggtgtatac acttgcacc 
      gcacgaaat attctcgat agtgtctcc ctgaatgta 
      actctagtg cttccagaa gtggtaaca gccaccaca 
      gtgagtaaa tccgagggt ggtttctta tggtggggc 
      ctgttggtg gctttagtg acccttgga agtgtgggc 
      ttaggggct agcattgct gtttgctgc ctccggggc 
      cgcaagacc cagaagcgc atgaaagcg agtgtgagc 
      ttcaccgag caggagaag aagctcctg gatgtgagt 
      atcgcaacg accacggac cgcggaacg ggaagttgc 
      gaagttctg ggtccggaa atggagctg gatcctccg 
      gtgcacatc accatcgag agcgagccg ctgccgctg 
      gcggtgttt ccgccgccc ccggagttc ggcaatgtg 
      gttccgtca gcgtacggc aacatcttc atttcagtg 
      tcagtgagt cgtgaccct tcggatgct tccagatgt 
      cctgacttg ttggacttg ccccacagg gccaagccg 
      gtgtaccat ggtatggcg acgctgccg cggcggccg 
      tgcgccgcc ccccactac gacaacatg ggcccccgc 
      gtcaccgcc gccggtagc tcgacgctg tcgctgccc 
      ggcgtgcct ccgccgccg ccgccgcca ccgcccccc 
      cagctcacc cccgagttt gtctctctt taa
    mRNA sequence
    • >TC008070 RA
      aggttcgaa agcggacgc tcgctagca gtcgcgttt 
      ccagcacgc acgctccac tctaaattt atcccgaaa 
      tatttccag tgctcgtaa aatgcgcgt gccgtcacg 
      atgagatgt tatggcggc ggtgatcct tctcctggt 
      gacggccat ggccctggc ggccccgga ctggaccga 
      ctgtccgtc gccgtgccg gtgcaagtg gtcgtcggg 
      caaaaagac ggcggtatg taagggggg cggcttctc 
      cgccgtccc cgacacgct cgatggcga gatgcaggt 
      gctggacct gtcggggaa ctacatctc tcggctggg 
      gaacgatgc gttcaaaag tgtcgggct gctgaacct 
      gcagcggat tttcctggc cacggccgg gatccagga 
      ggtccacaa ggacgcctt ccgggattt gactatttt 
      ggtcgaggt cgacttgtc gcataatca gatcaagag 
      tttgcatcc ggagacgtt tcacgggaa tgagcggct 
      acgcgtgtt gtatctcaa tgggaatcc gctgaggag 
      gctggtcca ggagcagtt tccccagct gccgcatct 
      ccggatttt ggagcttga tggctgtca gctggaata 
      tgttcacaa gaacgcttt cgtgcattt gagtgtgtt 
      ggagactct gagtctgag acagaattt attgcggaa 
      tctttcgga ggaggtttt tatgaattt tgctcattt 
      gaagacttt ggttttgga agggaatcc ttggaagtg 
      cgattgtga gttgcgcgg gtttaggga ttggtttct 
      cttgagtaa attgaattc ggtttcttt gacttgtac 
      tcagcctga acggcttga aggcaagtt gtgggaaaa 
      cgttctttc ggaagaatt cgcttgtcc gcctcgcgt 
      atcagtttt cccccaaag tcaagtcca ggctgaagc 
      tggcggaaa tgtcagttt tgggtgtca cgtgactgg 
      ggacccgga accccaagt ctcctggct ttatgaagg 
      ttatccgat aaatcatac gtggttagt gattcaagc 
      cgaagaagg acttttaga taagtgggc caatataag 
      tgtttacaa cgtcagtga tacagacac aggtgtata 
      cacttgcac cgcacgaaa tattctcga tagtgtctc 
      cctgaatgt aactctagt gcttccaga agtggtaac 
      agccaccac agtgagtaa atccgaggg tggtttctt 
      atggtgggg cctgttggt ggctttagt gacccttgg 
      aagtgtggg cttaggggc tagcattgc tgtttgctg 
      cctccgggg ccgcaagac ccagaagcg catgaaagc 
      gagtgtgag cttcaccga gcaggagaa gaagctcct 
      ggatgtgag tatcgcaac gaccacgga ccgcggaac 
      gggaagttg cgaagttct gggtccgga aatggagct 
      ggatcctcc ggtgcacat caccatcga gagcgagcc 
      gctgccgct ggcggtgtt tccgccgcc cccggagtt 
      cggcaatgt ggttccgtc agcgtacgg caacatctt 
      catttcagt gtcagtgag tcgtgaccc ttcggatgc 
      ttccagatg tcctgactt gttggactt gccccacag 
      ggccaagcc ggtgtacca tggtatggc gacgctgcc 
      gcggcggcc gtgcgccgc cccccacta cgacaacat 
      gggcccccg cgtcaccgc cgccggtag ctcgacgct 
      gtcgctgcc cggcgtgcc tccgccgcc gccgccgcc 
      accgccccc ccagctcac ccccgagtt tgtctctct 
      ttaacctga ctcctggaa atcttcaat tagtgcctt 
      tgctcacgt cacggtctc ttctgtttt tattattgt 
      atttcgcgt tctgtgtgt tccactcca cactctctt 
      attgtcggt catgtgtat acctttaga cttactggc 
      gccacaacg gagattatg agacttttt accatcttt 
      aacgtttct tttgttatt tgcgtattg ttgatctgt 
      tagataaat caaataaat taatctttg taat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY