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Gene information
General information
TC008033 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4112488..4122651
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008033
    iBeetle screen iB_07169

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008033 RA
      MQQSDYRMY STSSYPIDK RLSALSLST KQETPEYAI 
NRFSQKYDS YQEFLYRNN QQRRRLSST GEGSGDSST 
TTSGSSYGE REDGSEGRA ESDETEERE ERKPLEGQI 
TDYERQQVE TFFKGLKTQ VYVCGSLAN LYFKTTSDK 
DWQLVYTGI PVVVLDIGE SRARDKRRI QIALAERGT 
CFMLWSDTI DNLSAYRVA GNAFHTMCC SSDHTKHIG 
FSFDTAQAA DEMWSHIER LVSDPENIS LSTPGKKKK 
KKERKVPKP APLPPKSHI SQPCCFQHI TSVDRDDTN 
RYYSLKEFV PSGHPARII HALDD
    CDS sequence
    • >TC008033 RA
      atgcaacag agcgattac aggatgtac tcgacctcg 
tcttatccc atcgacaaa agactctcg gctttgtcc 
ttatcgacc aaacaggag actcccgaa tacgccata 
aaccgtttc tcgcagaaa tacgactcc taccaggag 
tttctctac aggaacaac cagcagagg cgtcggctc 
tcgtccaca ggggaagga agtggcgac tcgagcacg 
actacctcc ggaagtagt tacggggaa cgagaggat 
ggcagcgaa gggagggcc gaaagcgac gaaaccgaa 
gaacgggaa gaaaggaaa ccgctagag ggtcagatt 
acggactat gagaggcag caagtggag accttcttc 
aaagggctg aagacgcag gtgtacgtt tgcggctct 
ttggccaac ctgtacttc aaaaccacc tccgacaaa 
gactggcaa ctcgtctac accggcatc cccgtcgtg 
gtgctcgac atcggggag tcccgggcc cgggacaag 
cgccgcatc cagatcgcc ctggcagag cggggcacc 
tgcttcatg ctctggagc gacaccatc gacaacctg 
tccgcgtac cgggtcgca ggcaacgct ttccacacc 
atgtgctgc tcctccgac cacaccaag cacatcggc 
ttcagcttc gacacggcc caggccgcc gacgagatg 
tggtcgcac atcgagcga ttggtttcc gatccggaa 
aacatatcg ctttccacg ccagggaaa aagaagaag 
aagaaagag cggaaagtg ccaaagcct gcgcctctt 
cctcccaaa agccatatc tcgcagccg tgctgcttt 
cagcatatc acgtcggtc gacagggat gacacgaat 
aggtattac tcgctgaag gagttcgtg cccagcgga 
cacccagcg aggataatt cacgcgctt gatgactga
    mRNA sequence
    • >TC008033 RA
      cgcttgtcg taccggatc gacgaaccg gagctttgc 
gttacactc agtcagttt tacagttcg tggtcaggt 
ggaatacct gagtgagtg cacttccga gggaaaaaa 
tgagcattt ttgtgccgc gactagtgt gattcagtg 
cgttcccgg attttcccg gattatttc acaggagaa 
gatgcaaca gagcgatta caggatgta ctcgacctc 
gtcttatcc catcgacaa aagactctc ggctttgtc 
cttatcgac caaacagga gactcccga atacgccat 
aaaccgttt ctcgcagaa atacgactc ctaccagga 
gtttctcta caggaacaa ccagcagag gcgtcggct 
ctcgtccac aggggaagg aagtggcga ctcgagcac 
gactacctc cggaagtag ttacgggga acgagagga 
tggcagcga agggagggc cgaaagcga cgaaaccga 
agaacggga agaaaggaa accgctaga gggtcagat 
tacggacta tgagaggca gcaagtgga gaccttctt 
caaagggct gaagacgca ggtgtacgt ttgcggctc 
tttggccaa cctgtactt caaaaccac ctccgacaa 
agactggca actcgtcta caccggcat ccccgtcgt 
ggtgctcga catcgggga gtcccgggc ccgggacaa 
gcgccgcat ccagatcgc cctggcaga gcggggcac 
ctgcttcat gctctggag cgacaccat cgacaacct 
gtccgcgta ccgggtcgc aggcaacgc tttccacac 
catgtgctg ctcctccga ccacaccaa gcacatcgg 
cttcagctt cgacacggc ccaggccgc cgacgagat 
gtggtcgca catcgagcg attggtttc cgatccgga 
aaacatatc gctttccac gccagggaa aaagaagaa 
gaagaaaga gcggaaagt gccaaagcc tgcgcctct 
tcctcccaa aagccatat ctcgcagcc gtgctgctt 
tcagcatat cacgtcggt cgacaggga tgacacgaa 
taggtatta ctcgctgaa ggagttcgt gcccagcgg 
acacccagc gaggataat tcacgcgct tgatgactg 
attctgtgt ggacgagtt tatgtaacc agtgttgct 
tgtgtctag attaggttg ttatcaaag ctagtttgg 
taattctat tattgtaga agctgagat agaggatgt 
attttaaat taatctgtg ataccgttt caacattta 
ttttatatt acttaagtg ttttccttt gttagtaat 
aaaggactt actgtaagt gtagttaag ctgagaata 
aatatgttt caatttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY