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Gene information
General information
TC016192 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4776919..4806193
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC016192
    iBeetle screen iB_07165

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016192 RA
      MREAAEGHL TTEALMWNQ NLNERTISG MTSEDFRLR 
LNQVLRKEG YKIDNQRYP EGYQEAPLA YDAVWSVAL 
AFNKTMDRL HKYGKSLKE FNYNNKEIA DDIYSAINS 
TQFLGISGY VAFSSQGDR IALTQIEQV INGNYVVLG 
YYDTQADNL TWFDREVWH GGKVPQDRT IIRNVLRTV 
TLPLFICMC VISSVGIVI AIALVVFNI WNNHRRVIQ 
SSHPVCNTI MLFGIVVCL TAVFLLGLD GRFVSPGAY 
PAICQTRAW LLSTGFTLS FGAMFSKVW RVHRLTTKA 
KSDPKVRTF VAIPGWCCV SEWDNNLVV SFVVNVITR 
LDG
    CDS sequence
    • >TC016192 RA
      atgcgggaa gctgcagag ggtcatctg accacggaa 
gcgcttatg tggaaccag aatctcaac gaaaggacc 
atttcgggg atgacctcg gaggacttc cggctgcgc 
ctgaatcaa gtcctgcgg aaagaagga tacaagatc 
gataaccag cgttatcct gaaggatat caggaggct 
cctctggcc tacgatgcc gtctggtcg gtcgcttta 
gcgtttaat aaaaccatg gaccgtctg cacaaatat 
ggcaaaagc ttgaaggaa tttaattac aataataag 
gaaatagcc gacgatatt tactcagcc atcaactcg 
actcaattc ttgggaatt tccggttac gtagcgttc 
agctcacag ggcgaccga atcgccctg actcaaatc 
gagcaagtt ataaatggt aactatgtg gtgctgggt 
tattacgac acacaagcg gataatctt acttggttc 
gatcgagag gtctggcat ggtggaaaa gtgcctcaa 
gacagaaca atcatacga aatgtatta cggaccgtt 
accttgcca ctttttatt tgcatgtgt gttatttcc 
agtgtgggc atagtcata gcgatcgct ttggtcgtt 
ttcaacata tggaacaac cacagaaga gtaatccag 
tcttctcac cccgtgtgc aacactata atgcttttc 
ggaattgtc gtatgctta acggccgtt ttcctcctc 
ggcctcgac ggcagattc gtgtcccca ggtgcctat 
cccgccatt tgccagacc cgagcgtgg ctcctctcc 
accggcttc acgctctcc ttcggcgcg atgtttagc 
aaagtgtgg cgagttcat cggctcacc accaaagcc 
aaaagcgac cccaaagtc aggacgttt gttgccatc 
ccaggatgg tgttgtgtt agtgagtgg gataataat 
ttggtagtt agttttgta gttaatgtt ataacgaga 
ttggacggt taa
    mRNA sequence
    • >TC016192 RA
      caatttttt ttgtttcag gttggtacg aggacaatt 
ggtttgagg tgaacctgg aggaggaaa acatcgaat 
gtagcttgg agcagatgc gggaagctg cagagggtc 
atctgacca cggaagcgc ttatgtgga accagaatc 
tcaacgaaa ggaccattt cggggatga cctcggagg 
acttccggc tgcgcctga atcaagtcc tgcggaaag 
aaggataca agatcgata accagcgtt atcctgaag 
gatatcagg aggctcctc tggcctacg atgccgtct 
ggtcggtcg ctttagcgt ttaataaaa ccatggacc 
gtctgcaca aatatggca aaagcttga aggaattta 
attacaata ataaggaaa tagccgacg atatttact 
cagccatca actcgactc aattcttgg gaatttccg 
gttacgtag cgttcagct cacagggcg accgaatcg 
ccctgactc aaatcgagc aagttataa atggtaact 
atgtggtgc tgggttatt acgacacac aagcggata 
atcttactt ggttcgatc gagaggtct ggcatggtg 
gaaaagtgc ctcaagaca gaacaatca tacgaaatg 
tattacgga ccgttacct tgccacttt ttatttgca 
tgtgtgtta tttccagtg tgggcatag tcatagcga 
tcgctttgg tcgttttca acatatgga acaaccaca 
gaagagtaa tccagtctt ctcaccccg tgtgcaaca 
ctataatgc ttttcggaa ttgtcgtat gcttaacgg 
ccgttttcc tcctcggcc tcgacggca gattcgtgt 
ccccaggtg cctatcccg ccatttgcc agacccgag 
cgtggctcc tctccaccg gcttcacgc tctccttcg 
gcgcgatgt ttagcaaag tgtggcgag ttcatcggc 
tcaccacca aagccaaaa gcgacccca aagtcagga 
cgtttgttg ccatcccag gatggtgtt gtgttagtg 
agtgggata ataatttgg tagttagtt ttgtagtta 
atgttataa cgagattgg acggttaaa acgattcta 
aagaggttg tactgacga caaacaatg aattcatgt 
tattggcac gttgcagcg tttgcaaca tatcaactt 
ttggttcaa ctgacgctg gaattgcca aattctgtt 
cccggagct ctgattcag ttgaattgt tacgccaat 
agttgtgac cctatatta atgtctagc aatattatt 
acgtgttag ttgttaaaa tgctctaag tatgttatt 
tgctacttg attttatta cacgttgtc ttgtttagt 
tccgacgat gaggcaaat cgttaaagc aacttccag 
tttcacgtt ctaggctag gtacacact ctacacaag 
atacaataa tgtctcaca tttgaaagt tgttttaat 
gaagttgaa cgcacgatt tttgcagtc gtacgttgc 
tggaggaag aaagtgttg agaaacggt catacccca 
ttacggctc aaaatgagg tatgactgg gtttagtct 
ataaggcac tttatgtta gttgaatga caaactgtt 
tattgaaac tattatgta agtacataa gatgaataa 
gtattgaat agtacctat acattgcag ttttactgt 
cgaaatatg tgttcaaaa acccaatca gctacctaa 
tcttatgtc aaattaaaa tttaagtgt aacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY