Status
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Gene information
General information
TC016192 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4776919..4806193
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016192
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07165

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016192 RA
      MREAAEGHL TTEALMWNQ NLNERTISG MTSEDFRLR 
      LNQVLRKEG YKIDNQRYP EGYQEAPLA YDAVWSVAL 
      AFNKTMDRL HKYGKSLKE FNYNNKEIA DDIYSAINS 
      TQFLGISGY VAFSSQGDR IALTQIEQV INGNYVVLG 
      YYDTQADNL TWFDREVWH GGKVPQDRT IIRNVLRTV 
      TLPLFICMC VISSVGIVI AIALVVFNI WNNHRRVIQ 
      SSHPVCNTI MLFGIVVCL TAVFLLGLD GRFVSPGAY 
      PAICQTRAW LLSTGFTLS FGAMFSKVW RVHRLTTKA 
      KSDPKVRTF VAIPGWCCV SEWDNNLVV SFVVNVITR 
      LDG
    CDS sequence
    • >TC016192 RA
      atgcgggaa gctgcagag ggtcatctg accacggaa 
      gcgcttatg tggaaccag aatctcaac gaaaggacc 
      atttcgggg atgacctcg gaggacttc cggctgcgc 
      ctgaatcaa gtcctgcgg aaagaagga tacaagatc 
      gataaccag cgttatcct gaaggatat caggaggct 
      cctctggcc tacgatgcc gtctggtcg gtcgcttta 
      gcgtttaat aaaaccatg gaccgtctg cacaaatat 
      ggcaaaagc ttgaaggaa tttaattac aataataag 
      gaaatagcc gacgatatt tactcagcc atcaactcg 
      actcaattc ttgggaatt tccggttac gtagcgttc 
      agctcacag ggcgaccga atcgccctg actcaaatc 
      gagcaagtt ataaatggt aactatgtg gtgctgggt 
      tattacgac acacaagcg gataatctt acttggttc 
      gatcgagag gtctggcat ggtggaaaa gtgcctcaa 
      gacagaaca atcatacga aatgtatta cggaccgtt 
      accttgcca ctttttatt tgcatgtgt gttatttcc 
      agtgtgggc atagtcata gcgatcgct ttggtcgtt 
      ttcaacata tggaacaac cacagaaga gtaatccag 
      tcttctcac cccgtgtgc aacactata atgcttttc 
      ggaattgtc gtatgctta acggccgtt ttcctcctc 
      ggcctcgac ggcagattc gtgtcccca ggtgcctat 
      cccgccatt tgccagacc cgagcgtgg ctcctctcc 
      accggcttc acgctctcc ttcggcgcg atgtttagc 
      aaagtgtgg cgagttcat cggctcacc accaaagcc 
      aaaagcgac cccaaagtc aggacgttt gttgccatc 
      ccaggatgg tgttgtgtt agtgagtgg gataataat 
      ttggtagtt agttttgta gttaatgtt ataacgaga 
      ttggacggt taa
    mRNA sequence
    • >TC016192 RA
      caatttttt ttgtttcag gttggtacg aggacaatt 
      ggtttgagg tgaacctgg aggaggaaa acatcgaat 
      gtagcttgg agcagatgc gggaagctg cagagggtc 
      atctgacca cggaagcgc ttatgtgga accagaatc 
      tcaacgaaa ggaccattt cggggatga cctcggagg 
      acttccggc tgcgcctga atcaagtcc tgcggaaag 
      aaggataca agatcgata accagcgtt atcctgaag 
      gatatcagg aggctcctc tggcctacg atgccgtct 
      ggtcggtcg ctttagcgt ttaataaaa ccatggacc 
      gtctgcaca aatatggca aaagcttga aggaattta 
      attacaata ataaggaaa tagccgacg atatttact 
      cagccatca actcgactc aattcttgg gaatttccg 
      gttacgtag cgttcagct cacagggcg accgaatcg 
      ccctgactc aaatcgagc aagttataa atggtaact 
      atgtggtgc tgggttatt acgacacac aagcggata 
      atcttactt ggttcgatc gagaggtct ggcatggtg 
      gaaaagtgc ctcaagaca gaacaatca tacgaaatg 
      tattacgga ccgttacct tgccacttt ttatttgca 
      tgtgtgtta tttccagtg tgggcatag tcatagcga 
      tcgctttgg tcgttttca acatatgga acaaccaca 
      gaagagtaa tccagtctt ctcaccccg tgtgcaaca 
      ctataatgc ttttcggaa ttgtcgtat gcttaacgg 
      ccgttttcc tcctcggcc tcgacggca gattcgtgt 
      ccccaggtg cctatcccg ccatttgcc agacccgag 
      cgtggctcc tctccaccg gcttcacgc tctccttcg 
      gcgcgatgt ttagcaaag tgtggcgag ttcatcggc 
      tcaccacca aagccaaaa gcgacccca aagtcagga 
      cgtttgttg ccatcccag gatggtgtt gtgttagtg 
      agtgggata ataatttgg tagttagtt ttgtagtta 
      atgttataa cgagattgg acggttaaa acgattcta 
      aagaggttg tactgacga caaacaatg aattcatgt 
      tattggcac gttgcagcg tttgcaaca tatcaactt 
      ttggttcaa ctgacgctg gaattgcca aattctgtt 
      cccggagct ctgattcag ttgaattgt tacgccaat 
      agttgtgac cctatatta atgtctagc aatattatt 
      acgtgttag ttgttaaaa tgctctaag tatgttatt 
      tgctacttg attttatta cacgttgtc ttgtttagt 
      tccgacgat gaggcaaat cgttaaagc aacttccag 
      tttcacgtt ctaggctag gtacacact ctacacaag 
      atacaataa tgtctcaca tttgaaagt tgttttaat 
      gaagttgaa cgcacgatt tttgcagtc gtacgttgc 
      tggaggaag aaagtgttg agaaacggt catacccca 
      ttacggctc aaaatgagg tatgactgg gtttagtct 
      ataaggcac tttatgtta gttgaatga caaactgtt 
      tattgaaac tattatgta agtacataa gatgaataa 
      gtattgaat agtacctat acattgcag ttttactgt 
      cgaaatatg tgttcaaaa acccaatca gctacctaa 
      tcttatgtc aaattaaaa tttaagtgt aacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY