General information | |||
TC032795
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3783305..3788713 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC032795 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07161 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032795 RA
MIDADSERL IFQVNGAWT LPGFVCDFY IAMDVTCST SSIFNLVAI SIDRYIAVT QPIKYAKHK NNRRVWLTI ALVWMISAA IGSPIVLGL NNTPDRVAD ACLFYNSDF IIYSSLSSF YIPCIIMVF LYYNIFKAL RNRAIQARL NRKPLPSEI KPGSVIENV ANTRRLAET TLGPATIEE QATNTGSGS GDEDEEGAE ADCHVISND KSTEFILAT VVEEAATVV STINTGSDR DRSTGSKKE RKATAAARF TIYKVNKAS RKKREKSSA KKERKATKT LAIVLGVFL ICWVPFFTC NIMDAMCSK LNLPCQPGV AAFLLTTWL GYMNSFVNP VIYTIFNPE FRKAFKKLM FLGP
- >TC032795 RA
atgattgac gcagattca gaaaggctt atttttcag gtgaatggg gcttggaca ctgccgggc ttcgtctgc gacttctac atcgcgatg gacgtcaca tgctcaact tcttccatc ttcaacctg gtcgcgatc tcgatagat aggtacatc gccgtgacg cagccgata aaatacgcc aagcacaag aacaaccga agagtgtgg ttaaccatc gcccttgtg tggatgatc tcggcggcc atcggctcc cccatcgtc ctcggcttg aacaacacc cccgatcgt gtagcagat gcgtgccta ttctacaat agcgatttt atcatctat tccagcctt tcctccttc tacatcccc tgtatcatc atggttttc ctttattat aatatattt aaggcgctg aggaaccgt gcgatccag gcacgtctc aaccgcaag ccgctccct tccgaaatc aagcccgga tctgtgata gagaacgtg gcgaacacc agacgtttg gccgaaacg accctcggt ccagccacc atcgaagag caggccacc aacaccggc tcgggctcg ggggacgag gacgaagaa ggggccgaa gccgactgt cacgtcata tcaaacgac aagagcact gaattcatt ttagcaacg gtagtcgaa gaagccgca accgtcgta tcaacaata aatacggga tctgaccgc gacagatca acagggtcc aaaaaagag cgcaaagcg actgcagcg gcccgattc accatctac aaagtgaac aaagcgagt cgtaaaaaa cgcgagaaa tcgtcggca aaaaaggag aggaaggcc acgaaaaca ttagccatt gtgttgggc gtgttcctg atatgctgg gtccccttc ttcacgtgc aacatcatg gacgcgatg tgcagcaag ctgaatctg ccgtgccag ccgggggtg gcggccttc ctgctcacc acttggctg gggtacatg aacagtttc gtgaatcca gtcatctat accatattc aatccggag tttaggaaa gccttcaag aaattaatg ttcctcggg ccttaa
- >TC032795 RA
atcaatatc gcaatcccc gaggtatct agtcgtggc aggcagtcg ccgccgatg attgacgca gattcagaa aggcttatt tttcaggtg aatggggct tggacactg ccgggcttc gtctgcgac ttctacatc gcgatggac gtcacatgc tcaacttct tccatcttc aacctggtc gcgatctcg atagatagg tacatcgcc gtgacgcag ccgataaaa tacgccaag cacaagaac aaccgaaga gtgtggtta accatcgcc cttgtgtgg atgatctcg gcggccatc ggctccccc atcgtcctc ggcttgaac aacaccccc gatcgtgta gcagatgcg tgcctattc tacaatagc gattttatc atctattcc agcctttcc tccttctac atcccctgt atcatcatg gttttcctt tattataat atatttaag gcgctgagg aaccgtgcg atccaggca cgtctcaac cgcaagccg ctcccttcc gaaatcaag cccggatct gtgatagag aacgtggcg aacaccaga cgtttggcc gaaacgacc ctcggtcca gccaccatc gaagagcag gccaccaac accggctcg ggctcgggg gacgaggac gaagaaggg gccgaagcc gactgtcac gtcatatca aacgacaag agcactgaa ttcatttta gcaacggta gtcgaagaa gccgcaacc gtcgtatca acaataaat acgggatct gaccgcgac agatcaaca gggtccaaa aaagagcgc aaagcgact gcagcggcc cgattcacc atctacaaa gtgaacaaa gcgagtcgt aaaaaacgc gagaaatcg tcggcaaaa aaggagagg aaggccacg aaaacatta gccattgtg ttgggcgtg ttcctgata tgctgggtc cccttcttc acgtgcaac atcatggac gcgatgtgc agcaagctg aatctgccg tgccagccg ggggtggcg gccttcctg ctcaccact tggctgggg tacatgaac agtttcgtg aatccagtc atctatacc atattcaat ccggagttt aggaaagcc ttcaagaaa ttaatgttc ctcgggcct taatttaat tctaggttt gtaatatgt tcggttgga actctttta tatgagatt tctcttgct caatgtaca taaacgacg ttttttcgt aatcctgca ttgagttgg tcgcgttct tttcggctc aagcttcgt tacgtacct gaagactca gcggtttaa tttctcttt ctctttcat tcatcggta ggcgaataa gagatattc cgaatttat tatagacat atcatcaat tttcattcg aattcaatg cccacgcaa aacgagctt caatcattg ataataatt agtgttttt gtaacgttg actctctgt cagaacggg gtcaatcaa gtactttca ttccaaata tattctgtg atcgctcta aacttagat tagattaat tttaaaaga aacgtttga tattgttga gtttcgcgt tttgtaaat atcagattt tacagctgc tgtagaaca atacttggc gcccaacat cgtggcgat ctatgcaac cagcagtgc acgcccgat gtcgggaca ctgcatttt tcacttgcg ttctctctg tacatactt gtaaatacc aagttgcaa ataatttcg gtggtaatc agtattaga tcagtaaat tgtcctctg tgagccaaa gcaatattt gtaaaagtg cgtgtgttt gaggccaac gcgagcgtc cgacacaca ctttgagcc ccattttta caaaacttt gctcggata cttccatcc ctttgtaaa atcaaaaac cttacgctt attatcata aaatactgt aaaatagag tgtattata ttaggcgca ttgcaatgg acatatatc taatgttaa aataaagct atttcttat aa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
