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Gene information
General information
TC032795
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007490
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3783305..3788713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032795
    iBeetle screen iB_07161

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032795 RA
      MIDADSERL IFQVNGAWT LPGFVCDFY IAMDVTCST 
      SSIFNLVAI SIDRYIAVT QPIKYAKHK NNRRVWLTI 
      ALVWMISAA IGSPIVLGL NNTPDRVAD ACLFYNSDF 
      IIYSSLSSF YIPCIIMVF LYYNIFKAL RNRAIQARL 
      NRKPLPSEI KPGSVIENV ANTRRLAET TLGPATIEE 
      QATNTGSGS GDEDEEGAE ADCHVISND KSTEFILAT 
      VVEEAATVV STINTGSDR DRSTGSKKE RKATAAARF 
      TIYKVNKAS RKKREKSSA KKERKATKT LAIVLGVFL 
      ICWVPFFTC NIMDAMCSK LNLPCQPGV AAFLLTTWL 
      GYMNSFVNP VIYTIFNPE FRKAFKKLM FLGP
    CDS sequence
    • >TC032795 RA
      atgattgac gcagattca gaaaggctt atttttcag 
      gtgaatggg gcttggaca ctgccgggc ttcgtctgc 
      gacttctac atcgcgatg gacgtcaca tgctcaact 
      tcttccatc ttcaacctg gtcgcgatc tcgatagat 
      aggtacatc gccgtgacg cagccgata aaatacgcc 
      aagcacaag aacaaccga agagtgtgg ttaaccatc 
      gcccttgtg tggatgatc tcggcggcc atcggctcc 
      cccatcgtc ctcggcttg aacaacacc cccgatcgt 
      gtagcagat gcgtgccta ttctacaat agcgatttt 
      atcatctat tccagcctt tcctccttc tacatcccc 
      tgtatcatc atggttttc ctttattat aatatattt 
      aaggcgctg aggaaccgt gcgatccag gcacgtctc 
      aaccgcaag ccgctccct tccgaaatc aagcccgga 
      tctgtgata gagaacgtg gcgaacacc agacgtttg 
      gccgaaacg accctcggt ccagccacc atcgaagag 
      caggccacc aacaccggc tcgggctcg ggggacgag 
      gacgaagaa ggggccgaa gccgactgt cacgtcata 
      tcaaacgac aagagcact gaattcatt ttagcaacg 
      gtagtcgaa gaagccgca accgtcgta tcaacaata 
      aatacggga tctgaccgc gacagatca acagggtcc 
      aaaaaagag cgcaaagcg actgcagcg gcccgattc 
      accatctac aaagtgaac aaagcgagt cgtaaaaaa 
      cgcgagaaa tcgtcggca aaaaaggag aggaaggcc 
      acgaaaaca ttagccatt gtgttgggc gtgttcctg 
      atatgctgg gtccccttc ttcacgtgc aacatcatg 
      gacgcgatg tgcagcaag ctgaatctg ccgtgccag 
      ccgggggtg gcggccttc ctgctcacc acttggctg 
      gggtacatg aacagtttc gtgaatcca gtcatctat 
      accatattc aatccggag tttaggaaa gccttcaag 
      aaattaatg ttcctcggg ccttaa
    mRNA sequence
    • >TC032795 RA
      atcaatatc gcaatcccc gaggtatct agtcgtggc 
      aggcagtcg ccgccgatg attgacgca gattcagaa 
      aggcttatt tttcaggtg aatggggct tggacactg 
      ccgggcttc gtctgcgac ttctacatc gcgatggac 
      gtcacatgc tcaacttct tccatcttc aacctggtc 
      gcgatctcg atagatagg tacatcgcc gtgacgcag 
      ccgataaaa tacgccaag cacaagaac aaccgaaga 
      gtgtggtta accatcgcc cttgtgtgg atgatctcg 
      gcggccatc ggctccccc atcgtcctc ggcttgaac 
      aacaccccc gatcgtgta gcagatgcg tgcctattc 
      tacaatagc gattttatc atctattcc agcctttcc 
      tccttctac atcccctgt atcatcatg gttttcctt 
      tattataat atatttaag gcgctgagg aaccgtgcg 
      atccaggca cgtctcaac cgcaagccg ctcccttcc 
      gaaatcaag cccggatct gtgatagag aacgtggcg 
      aacaccaga cgtttggcc gaaacgacc ctcggtcca 
      gccaccatc gaagagcag gccaccaac accggctcg 
      ggctcgggg gacgaggac gaagaaggg gccgaagcc 
      gactgtcac gtcatatca aacgacaag agcactgaa 
      ttcatttta gcaacggta gtcgaagaa gccgcaacc 
      gtcgtatca acaataaat acgggatct gaccgcgac 
      agatcaaca gggtccaaa aaagagcgc aaagcgact 
      gcagcggcc cgattcacc atctacaaa gtgaacaaa 
      gcgagtcgt aaaaaacgc gagaaatcg tcggcaaaa 
      aaggagagg aaggccacg aaaacatta gccattgtg 
      ttgggcgtg ttcctgata tgctgggtc cccttcttc 
      acgtgcaac atcatggac gcgatgtgc agcaagctg 
      aatctgccg tgccagccg ggggtggcg gccttcctg 
      ctcaccact tggctgggg tacatgaac agtttcgtg 
      aatccagtc atctatacc atattcaat ccggagttt 
      aggaaagcc ttcaagaaa ttaatgttc ctcgggcct 
      taatttaat tctaggttt gtaatatgt tcggttgga 
      actctttta tatgagatt tctcttgct caatgtaca 
      taaacgacg ttttttcgt aatcctgca ttgagttgg 
      tcgcgttct tttcggctc aagcttcgt tacgtacct 
      gaagactca gcggtttaa tttctcttt ctctttcat 
      tcatcggta ggcgaataa gagatattc cgaatttat 
      tatagacat atcatcaat tttcattcg aattcaatg 
      cccacgcaa aacgagctt caatcattg ataataatt 
      agtgttttt gtaacgttg actctctgt cagaacggg 
      gtcaatcaa gtactttca ttccaaata tattctgtg 
      atcgctcta aacttagat tagattaat tttaaaaga 
      aacgtttga tattgttga gtttcgcgt tttgtaaat 
      atcagattt tacagctgc tgtagaaca atacttggc 
      gcccaacat cgtggcgat ctatgcaac cagcagtgc 
      acgcccgat gtcgggaca ctgcatttt tcacttgcg 
      ttctctctg tacatactt gtaaatacc aagttgcaa 
      ataatttcg gtggtaatc agtattaga tcagtaaat 
      tgtcctctg tgagccaaa gcaatattt gtaaaagtg 
      cgtgtgttt gaggccaac gcgagcgtc cgacacaca 
      ctttgagcc ccattttta caaaacttt gctcggata 
      cttccatcc ctttgtaaa atcaaaaac cttacgctt 
      attatcata aaatactgt aaaatagag tgtattata 
      ttaggcgca ttgcaatgg acatatatc taatgttaa 
      aataaagct atttcttat aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY