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Gene information
General information
TC032795
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007490 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3783305..3788713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032795
    iBeetle screen iB_07161

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032795 RA
      MIDADSERL IFQVNGAWT LPGFVCDFY IAMDVTCST 
SSIFNLVAI SIDRYIAVT QPIKYAKHK NNRRVWLTI 
ALVWMISAA IGSPIVLGL NNTPDRVAD ACLFYNSDF 
IIYSSLSSF YIPCIIMVF LYYNIFKAL RNRAIQARL 
NRKPLPSEI KPGSVIENV ANTRRLAET TLGPATIEE 
QATNTGSGS GDEDEEGAE ADCHVISND KSTEFILAT 
VVEEAATVV STINTGSDR DRSTGSKKE RKATAAARF 
TIYKVNKAS RKKREKSSA KKERKATKT LAIVLGVFL 
ICWVPFFTC NIMDAMCSK LNLPCQPGV AAFLLTTWL 
GYMNSFVNP VIYTIFNPE FRKAFKKLM FLGP
    CDS sequence
    • >TC032795 RA
      atgattgac gcagattca gaaaggctt atttttcag 
gtgaatggg gcttggaca ctgccgggc ttcgtctgc 
gacttctac atcgcgatg gacgtcaca tgctcaact 
tcttccatc ttcaacctg gtcgcgatc tcgatagat 
aggtacatc gccgtgacg cagccgata aaatacgcc 
aagcacaag aacaaccga agagtgtgg ttaaccatc 
gcccttgtg tggatgatc tcggcggcc atcggctcc 
cccatcgtc ctcggcttg aacaacacc cccgatcgt 
gtagcagat gcgtgccta ttctacaat agcgatttt 
atcatctat tccagcctt tcctccttc tacatcccc 
tgtatcatc atggttttc ctttattat aatatattt 
aaggcgctg aggaaccgt gcgatccag gcacgtctc 
aaccgcaag ccgctccct tccgaaatc aagcccgga 
tctgtgata gagaacgtg gcgaacacc agacgtttg 
gccgaaacg accctcggt ccagccacc atcgaagag 
caggccacc aacaccggc tcgggctcg ggggacgag 
gacgaagaa ggggccgaa gccgactgt cacgtcata 
tcaaacgac aagagcact gaattcatt ttagcaacg 
gtagtcgaa gaagccgca accgtcgta tcaacaata 
aatacggga tctgaccgc gacagatca acagggtcc 
aaaaaagag cgcaaagcg actgcagcg gcccgattc 
accatctac aaagtgaac aaagcgagt cgtaaaaaa 
cgcgagaaa tcgtcggca aaaaaggag aggaaggcc 
acgaaaaca ttagccatt gtgttgggc gtgttcctg 
atatgctgg gtccccttc ttcacgtgc aacatcatg 
gacgcgatg tgcagcaag ctgaatctg ccgtgccag 
ccgggggtg gcggccttc ctgctcacc acttggctg 
gggtacatg aacagtttc gtgaatcca gtcatctat 
accatattc aatccggag tttaggaaa gccttcaag 
aaattaatg ttcctcggg ccttaa
    mRNA sequence
    • >TC032795 RA
      atcaatatc gcaatcccc gaggtatct agtcgtggc 
aggcagtcg ccgccgatg attgacgca gattcagaa 
aggcttatt tttcaggtg aatggggct tggacactg 
ccgggcttc gtctgcgac ttctacatc gcgatggac 
gtcacatgc tcaacttct tccatcttc aacctggtc 
gcgatctcg atagatagg tacatcgcc gtgacgcag 
ccgataaaa tacgccaag cacaagaac aaccgaaga 
gtgtggtta accatcgcc cttgtgtgg atgatctcg 
gcggccatc ggctccccc atcgtcctc ggcttgaac 
aacaccccc gatcgtgta gcagatgcg tgcctattc 
tacaatagc gattttatc atctattcc agcctttcc 
tccttctac atcccctgt atcatcatg gttttcctt 
tattataat atatttaag gcgctgagg aaccgtgcg 
atccaggca cgtctcaac cgcaagccg ctcccttcc 
gaaatcaag cccggatct gtgatagag aacgtggcg 
aacaccaga cgtttggcc gaaacgacc ctcggtcca 
gccaccatc gaagagcag gccaccaac accggctcg 
ggctcgggg gacgaggac gaagaaggg gccgaagcc 
gactgtcac gtcatatca aacgacaag agcactgaa 
ttcatttta gcaacggta gtcgaagaa gccgcaacc 
gtcgtatca acaataaat acgggatct gaccgcgac 
agatcaaca gggtccaaa aaagagcgc aaagcgact 
gcagcggcc cgattcacc atctacaaa gtgaacaaa 
gcgagtcgt aaaaaacgc gagaaatcg tcggcaaaa 
aaggagagg aaggccacg aaaacatta gccattgtg 
ttgggcgtg ttcctgata tgctgggtc cccttcttc 
acgtgcaac atcatggac gcgatgtgc agcaagctg 
aatctgccg tgccagccg ggggtggcg gccttcctg 
ctcaccact tggctgggg tacatgaac agtttcgtg 
aatccagtc atctatacc atattcaat ccggagttt 
aggaaagcc ttcaagaaa ttaatgttc ctcgggcct 
taatttaat tctaggttt gtaatatgt tcggttgga 
actctttta tatgagatt tctcttgct caatgtaca 
taaacgacg ttttttcgt aatcctgca ttgagttgg 
tcgcgttct tttcggctc aagcttcgt tacgtacct 
gaagactca gcggtttaa tttctcttt ctctttcat 
tcatcggta ggcgaataa gagatattc cgaatttat 
tatagacat atcatcaat tttcattcg aattcaatg 
cccacgcaa aacgagctt caatcattg ataataatt 
agtgttttt gtaacgttg actctctgt cagaacggg 
gtcaatcaa gtactttca ttccaaata tattctgtg 
atcgctcta aacttagat tagattaat tttaaaaga 
aacgtttga tattgttga gtttcgcgt tttgtaaat 
atcagattt tacagctgc tgtagaaca atacttggc 
gcccaacat cgtggcgat ctatgcaac cagcagtgc 
acgcccgat gtcgggaca ctgcatttt tcacttgcg 
ttctctctg tacatactt gtaaatacc aagttgcaa 
ataatttcg gtggtaatc agtattaga tcagtaaat 
tgtcctctg tgagccaaa gcaatattt gtaaaagtg 
cgtgtgttt gaggccaac gcgagcgtc cgacacaca 
ctttgagcc ccattttta caaaacttt gctcggata 
cttccatcc ctttgtaaa atcaaaaac cttacgctt 
attatcata aaatactgt aaaatagag tgtattata 
ttaggcgca ttgcaatgg acatatatc taatgttaa 
aataaagct atttcttat aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY