Status
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Gene information
General information
TC008001 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3716985..3732296
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008001
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07158

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008001 RA
      MSNFCNPEP PTGPSNPPT APPPPPPPA QCSTYTFPE 
      VRSPESRGH LRSISHGGS TSLGGSSSA GVVGRPSAL 
      KGGRGHQRA FSQGQIGAE AVRPGHSRV GSKTDFILP 
      PGHKDSENV VGGSVSKVS AKGHSRQAS RSESIYTLR 
      RSAPPPWWR RLLCVLLRR SPKSEPEER YRIVVPNHL 
      VPPKTPRKE HPNGKRPNN KIRTTKYTL LSFLPRNLL 
      EQFHRVANL YFIFIVLLN WFPAINAFG KEIAMIPVM 
      FVLGVTAVK DLFEDRRRH ASDKRINNS TCRIYQSEA 
      ARYKKVLWK EVRVGDLIH LSNNEVVPA DILLLRSSD 
      PSGLCYIDT GHLDGETNL KQRQVARGF VSKQDVFEP 
      SKFRSKVEV EAPTTKIYR FHGAIVHPS GERVPVGTD 
      NILLRECLL KNTDFVEGI VVYAGHETK AMLNNGGPR 
      YKRSSLERQ MNQDVLWCV LILIVLCLI GAIGCKLWL 
      NIFNRLESP FKAEHSEGT EAFLAFWTY IIILQIMIP 
      LSLYVTLEM CKIIQVYHI HHNVDLYDS IMDKRTECR 
      ALNITEELG QIQYIFSDK TGTLTENRM IFRRCAIAG 
      VDYNHPKME DEGKNGKLT SLSVVANPR LLSDLNQVG 
      AVTEQIYTT RQTHAAKIQ EFLLLLAVC NTVVCSRHP 
      HYDMMNASG LIEAVPSSF SQSDKYTRL EESRSVTPS 
      PPLNYLEGR TTELSCSSS DVEVKKPKL LSLPLLGRK 
      QGSNTNLTD EQKAKLRSS TPSPHDLKP IFEAESPDE 
      LALVDAAFN YNCKLLKRT PTSVCVEFF DEGRVTYEI 
      LDVLPFDSI RKRMSVIVK HPVSGEIIL FCKGADSSI 
      FPYLAPVED DSEQKILIN KTQNHVNNY SKDGLRILL 
      MAKRVLSLQ EYTEWSKKQ RELNMSLDN FERKVQESY 
      SRIECNLTL LGATGIEDR LQEGVPETL SALMSAGIV 
      VWVLTGDKP ETAINIAYS SKLFSPQME LLKLMTRSK 
      ETAESTIMF YLSEIENAA SSIQTVLPT TSRRDLNRS 
      GPKNRSKAL VIDGKTLTF ILDRRSNLT KPFLKLTAH 
      CSSVLCCRA TPLQKAYIV KVVKEELKM RTLAIGDGA 
      NDVSMIQTA DVGIGISGQ EGMQAVMAA DFALSRFKF 
      LERLLLVHG HWSYDRLSR MVLYFFYKN AAFVFLCFW 
      YQLFCGFSG SVMIDQMYL MLYNLLFTS LPPLAIGVY 
      DQDAPEELL RDRPYLYRQ GRLGKVYKS YSFWLTMAD 
      AIYQSLCIF FICQGAYAD SDIDMFEFG TTATTACMF 
      VMLLHVSIE IRSWTIIHV GSIVISIGA FYVYSFLYN 
      SLCVNCFGL PSTYWIIQK ATSRPTYWL VTLLSCVVA 
      LLPRYVFRV LKTLLAPDD VTRAVMAYR RASRREPQI 
      RGLRITL
    CDS sequence
    • >TC008001 RA
      atgtcaaac ttctgcaac cccgagccg cccaccggt 
      ccgtcaaac cctccgacg gcgcctcca ccaccacca 
      cccccggcc caatgctcc acgtacaca tttcctgaa 
      gtccgttcc cctgaaagc cgtggccac ctccgtagc 
      atcagccat ggaggtagt acttctctt ggtggtagc 
      agttcagcg ggtgtcgtt ggccggccg tcggccctc 
      aaagggggc cgcggacac caacgtgct ttctcacaa 
      ggccaaatc ggggctgaa gccgttcgg cccggccat 
      agccgagtt ggttccaaa actgacttc atactacct 
      cctggacat aaagacagc gaaaatgtc gtcgggggt 
      agtgtctcg aaggtctcc gccaagggg cacagtcgg 
      caggcgtcg cgatcggag agtatttat accttgcgg 
      cggtcggcg ccgcccccg tggtggcgg cggttgctg 
      tgtgtactg ctgaggcgg tcacccaag tccgagccg 
      gaggaaagg tataggata gtggtgccg aaccaccta 
      gtgcccccc aagacgccg cggaaggag cacccgaac 
      ggcaagagg ccgaataat aaaatcagg actacaaaa 
      tatacgctt ttgtcgttt ttgccgcga aatttgctc 
      gaacagttt catcgcgtc gcgaatttg tattttatt 
      tttatcgtg ttgctcaat tggtttccg gcgataaat 
      gcgttcggg aaggagatc gcgatgatt ccggttatg 
      ttcgtgtta ggtgtgacg gctgtgaag gatttattc 
      gaagatcgc agaaggcat gcgagtgat aagaggata 
      aataactcg acatgtagg atatatcag agtgaggcc 
      gccagatat aaaaaagtt ctctggaag gaagtcaga 
      gttggcgat ttgatccat ctctcgaac aacgaagtc 
      gtcccagct gatattctt ctgctgcga tctagtgac 
      ccgagcggc ttgtgttac attgacacg ggccatctt 
      gacggtgaa acgaatttg aagcaacgt caagtcgcc 
      cgcggattc gtttcaaaa caagatgtg ttcgaacct 
      agcaaattt cgcagcaaa gtggaagtg gaagcgccc 
      acaactaaa atctatcgt tttcatggg gctatcgtc 
      cacccgtcg ggtgagaga gtgcccgtg ggcactgac 
      aatatacta ctcagggag tgccttctc aaaaatacc 
      gattttgtg gaaggaatc gtcgtttat gcgggtcac 
      gagaccaaa gcaatgctc aataacgga ggaccaaga 
      tataaaaga tcttccttg gaacgacaa atgaatcag 
      gacgtgctc tggtgcgtc ttgattctg atcgttctg 
      tgtctgatc ggtgccata gggtgtaag ctttggttg 
      aacattttt aataggttg gagtctccg ttcaaggcc 
      gagcactcc gaagggacg gaggcgttt ttagcattt 
      tggacctat atcatcatt ttacagata atgatccct 
      ttatcgctg tacgtaacc ctcgaaatg tgcaaaatc 
      atccaagtg taccacatc caccacaac gtggacctc 
      tacgactcc atcatggac aagcgcacc gaatgccgc 
      gccctcaac atcaccgaa gagctcggc caaatccag 
      tacatcttc tcggacaaa acgggcact ttgaccgaa 
      aaccggatg attttccgt cgttgcgcc atcgccggc 
      gtcgactac aaccacccg aaaatggaa gacgagggc 
      aagaacggt aaactcacc tccctcagc gtggtggca 
      aacccgcgc ctcctcagc gacctcaac caagtcggc 
      gccgtcacc gaacaaatc tacaccaca cgccaaacc 
      cacgccgcc aaaatccag gaattttta cttttacta 
      gccgtttgt aacacagtc gtgtgctcg agacacccc 
      cattacgac atgatgaac gcgtcgggt ttgatcgag 
      gcggtgccg agctcgttc agccaatcg gataagtac 
      acgcggctg gaggagtct cgttcggtg acgccctcc 
      ccacctttg aattatctc gaggggcgg acgaccgaa 
      ctgtcgtgt tcgtcgtcc gatgttgag gtgaagaaa 
      cccaaactt ttgagtttg ccgcttttg gggcgcaaa 
      caagggagt aacacgaat ttaactgac gaacagaag 
      gccaagctg aggtcgtcg acgccgagt ccgcacgat 
      ttgaagccg atttttgaa gccgaaagt ccggacgag 
      ttggcgttg gtcgacgcc gcctttaat tacaactgc 
      aagttgttg aaacggaca ccgacgtcg gtctgtgtc 
      gagtttttc gatgagggc cgagtgact tacgagatt 
      ttggacgtt ttaccgttt gattcaatc cggaaacgg 
      atgtctgtc atcgtcaaa catcccgtt tctggggaa 
      attatttta ttttgtaag ggggccgat agtagcatt 
      tttccctat ttggcccca gttgaggac gattccgaa 
      caaaaaatt ctaatcaac aagacgcaa aaccacgtc 
      aataattat tcgaaggat gggcttagg attttacta 
      atggccaag cgggtgttg tcgctccaa gaatataca 
      gagtggagt aaaaaacaa agggagttg aacatgtca 
      cttgataat ttcgagcgt aaggttcag gagagttat 
      tcacgaatt gagtgcaat ttaactttg ttgggggct 
      acggggatt gaggaccgg ctccaggag ggggtccct 
      gagactctt tcggccctc atgtcggca gggattgtc 
      gtttgggtt ctgaccgga gataagcca gaaaccgcc 
      ataaacata gcctactct agtaaatta ttctcgcca 
      caaatggag ttgttaaaa ctcatgaca cggtcaaag 
      gaaacggcc gaaagcacg atcatgttc tacttatca 
      gagattgaa aacgcagcc tctagtatc caaacggtg 
      ttgccgacc accagccgc cgtgatttg aaccgttcg 
      gggccgaaa aaccgaagt aaggcactg gtgattgac 
      ggaaaaacc ctgactttt atccttgac cggcgctca 
      aacctaact aaaccgttt ctgaaactg accgctcac 
      tgtagttcg gttttgtgt tgtcgggcg actccgctc 
      cagaaagct tatatagtt aaagtggtt aaggaagag 
      ttgaaaatg cggactttg gcgatcggg gacggggct 
      aatgacgtg tcgatgatc cagacggcc gatgtcggg 
      atcgggatt tcgggccag gaagggatg caggccgtc 
      atggcggcc gactttgcc ttatcccgg ttcaagttt 
      ctggagcgg ttgttgctc gtccacgga cattggagt 
      tacgatcgg ttgtcgcgc atggttttg tattttttc 
      tacaagaat gcggcgttc gtgtttttg tgtttttgg 
      tatcagttg ttttgcggg ttttccggc tcggttatg 
      atcgatcag atgtatctc atgttgtat aatttgttg 
      tttacgtcg ttgccgccg cttgcgatt ggagtgtac 
      gatcaggac gctcctgag gaactcttg agggatagg 
      ccgtacctg tatagacag ggccggttg gggaaagtc 
      tacaagagt tattcgttt tggttgacg atggccgat 
      gccatttac caaagtctt tgtattttt ttcatctgt 
      cagggggct tatgctgac tctgatatc gatatgttt 
      gagtttggg accacggcg acgacggcg tgcatgttt 
      gtcatgttg ttgcacgtc tccattgag atacggtct 
      tggaccata atccacgtg ggttcgatc gtgatttcg 
      attggggcg ttttacgtc tattcgttc ctgtacaat 
      tccctgtgc gtcaattgt ttcggcttg cctagcacg 
      tattggata atccagaag gcgacgagt cggcccact 
      tactggctg gtgactttg ctgtcgtgc gttgtcgct 
      ttgttgccg aggtatgtg ttccgggtg ttgaagacg 
      ctgctggcg cctgatgac gtgacgagg gccgtgatg 
      gcatatcgc agggctagt agaagagaa ccacagatt 
      cgaggccta agaataacc ctttga
    mRNA sequence
    • >TC008001 RA
      gttcagtga aaatagttc gacgtgtgc gagtttaga 
      agttggtgt ttgcgtgtc ctgaacctt attttttag 
      tatcacacg tgtgacttc attttccag tgattaatt 
      tacgtgtaa atctgaaag gaagtcgtc cggatctgg 
      atttcagtc aagttatga aagataaga acgcaaacg 
      atgtcaaac ttctgcaac cccgagccg cccaccggt 
      ccgtcaaac cctccgacg gcgcctcca ccaccacca 
      cccccggcc caatgctcc acgtacaca tttcctgaa 
      gtccgttcc cctgaaagc cgtggccac ctccgtagc 
      atcagccat ggaggtagt acttctctt ggtggtagc 
      agttcagcg ggtgtcgtt ggccggccg tcggccctc 
      aaagggggc cgcggacac caacgtgct ttctcacaa 
      ggccaaatc ggggctgaa gccgttcgg cccggccat 
      agccgagtt ggttccaaa actgacttc atactacct 
      cctggacat aaagacagc gaaaatgtc gtcgggggt 
      agtgtctcg aaggtctcc gccaagggg cacagtcgg 
      caggcgtcg cgatcggag agtatttat accttgcgg 
      cggtcggcg ccgcccccg tggtggcgg cggttgctg 
      tgtgtactg ctgaggcgg tcacccaag tccgagccg 
      gaggaaagg tataggata gtggtgccg aaccaccta 
      gtgcccccc aagacgccg cggaaggag cacccgaac 
      ggcaagagg ccgaataat aaaatcagg actacaaaa 
      tatacgctt ttgtcgttt ttgccgcga aatttgctc 
      gaacagttt catcgcgtc gcgaatttg tattttatt 
      tttatcgtg ttgctcaat tggtttccg gcgataaat 
      gcgttcggg aaggagatc gcgatgatt ccggttatg 
      ttcgtgtta ggtgtgacg gctgtgaag gatttattc 
      gaagatcgc agaaggcat gcgagtgat aagaggata 
      aataactcg acatgtagg atatatcag agtgaggcc 
      gccagatat aaaaaagtt ctctggaag gaagtcaga 
      gttggcgat ttgatccat ctctcgaac aacgaagtc 
      gtcccagct gatattctt ctgctgcga tctagtgac 
      ccgagcggc ttgtgttac attgacacg ggccatctt 
      gacggtgaa acgaatttg aagcaacgt caagtcgcc 
      cgcggattc gtttcaaaa caagatgtg ttcgaacct 
      agcaaattt cgcagcaaa gtggaagtg gaagcgccc 
      acaactaaa atctatcgt tttcatggg gctatcgtc 
      cacccgtcg ggtgagaga gtgcccgtg ggcactgac 
      aatatacta ctcagggag tgccttctc aaaaatacc 
      gattttgtg gaaggaatc gtcgtttat gcgggtcac 
      gagaccaaa gcaatgctc aataacgga ggaccaaga 
      tataaaaga tcttccttg gaacgacaa atgaatcag 
      gacgtgctc tggtgcgtc ttgattctg atcgttctg 
      tgtctgatc ggtgccata gggtgtaag ctttggttg 
      aacattttt aataggttg gagtctccg ttcaaggcc 
      gagcactcc gaagggacg gaggcgttt ttagcattt 
      tggacctat atcatcatt ttacagata atgatccct 
      ttatcgctg tacgtaacc ctcgaaatg tgcaaaatc 
      atccaagtg taccacatc caccacaac gtggacctc 
      tacgactcc atcatggac aagcgcacc gaatgccgc 
      gccctcaac atcaccgaa gagctcggc caaatccag 
      tacatcttc tcggacaaa acgggcact ttgaccgaa 
      aaccggatg attttccgt cgttgcgcc atcgccggc 
      gtcgactac aaccacccg aaaatggaa gacgagggc 
      aagaacggt aaactcacc tccctcagc gtggtggca 
      aacccgcgc ctcctcagc gacctcaac caagtcggc 
      gccgtcacc gaacaaatc tacaccaca cgccaaacc 
      cacgccgcc aaaatccag gaattttta cttttacta 
      gccgtttgt aacacagtc gtgtgctcg agacacccc 
      cattacgac atgatgaac gcgtcgggt ttgatcgag 
      gcggtgccg agctcgttc agccaatcg gataagtac 
      acgcggctg gaggagtct cgttcggtg acgccctcc 
      ccacctttg aattatctc gaggggcgg acgaccgaa 
      ctgtcgtgt tcgtcgtcc gatgttgag gtgaagaaa 
      cccaaactt ttgagtttg ccgcttttg gggcgcaaa 
      caagggagt aacacgaat ttaactgac gaacagaag 
      gccaagctg aggtcgtcg acgccgagt ccgcacgat 
      ttgaagccg atttttgaa gccgaaagt ccggacgag 
      ttggcgttg gtcgacgcc gcctttaat tacaactgc 
      aagttgttg aaacggaca ccgacgtcg gtctgtgtc 
      gagtttttc gatgagggc cgagtgact tacgagatt 
      ttggacgtt ttaccgttt gattcaatc cggaaacgg 
      atgtctgtc atcgtcaaa catcccgtt tctggggaa 
      attatttta ttttgtaag ggggccgat agtagcatt 
      tttccctat ttggcccca gttgaggac gattccgaa 
      caaaaaatt ctaatcaac aagacgcaa aaccacgtc 
      aataattat tcgaaggat gggcttagg attttacta 
      atggccaag cgggtgttg tcgctccaa gaatataca 
      gagtggagt aaaaaacaa agggagttg aacatgtca 
      cttgataat ttcgagcgt aaggttcag gagagttat 
      tcacgaatt gagtgcaat ttaactttg ttgggggct 
      acggggatt gaggaccgg ctccaggag ggggtccct 
      gagactctt tcggccctc atgtcggca gggattgtc 
      gtttgggtt ctgaccgga gataagcca gaaaccgcc 
      ataaacata gcctactct agtaaatta ttctcgcca 
      caaatggag ttgttaaaa ctcatgaca cggtcaaag 
      gaaacggcc gaaagcacg atcatgttc tacttatca 
      gagattgaa aacgcagcc tctagtatc caaacggtg 
      ttgccgacc accagccgc cgtgatttg aaccgttcg 
      gggccgaaa aaccgaagt aaggcactg gtgattgac 
      ggaaaaacc ctgactttt atccttgac cggcgctca 
      aacctaact aaaccgttt ctgaaactg accgctcac 
      tgtagttcg gttttgtgt tgtcgggcg actccgctc 
      cagaaagct tatatagtt aaagtggtt aaggaagag 
      ttgaaaatg cggactttg gcgatcggg gacggggct 
      aatgacgtg tcgatgatc cagacggcc gatgtcggg 
      atcgggatt tcgggccag gaagggatg caggccgtc 
      atggcggcc gactttgcc ttatcccgg ttcaagttt 
      ctggagcgg ttgttgctc gtccacgga cattggagt 
      tacgatcgg ttgtcgcgc atggttttg tattttttc 
      tacaagaat gcggcgttc gtgtttttg tgtttttgg 
      tatcagttg ttttgcggg ttttccggc tcggttatg 
      atcgatcag atgtatctc atgttgtat aatttgttg 
      tttacgtcg ttgccgccg cttgcgatt ggagtgtac 
      gatcaggac gctcctgag gaactcttg agggatagg 
      ccgtacctg tatagacag ggccggttg gggaaagtc 
      tacaagagt tattcgttt tggttgacg atggccgat 
      gccatttac caaagtctt tgtattttt ttcatctgt 
      cagggggct tatgctgac tctgatatc gatatgttt 
      gagtttggg accacggcg acgacggcg tgcatgttt 
      gtcatgttg ttgcacgtc tccattgag atacggtct 
      tggaccata atccacgtg ggttcgatc gtgatttcg 
      attggggcg ttttacgtc tattcgttc ctgtacaat 
      tccctgtgc gtcaattgt ttcggcttg cctagcacg 
      tattggata atccagaag gcgacgagt cggcccact 
      tactggctg gtgactttg ctgtcgtgc gttgtcgct 
      ttgttgccg aggtatgtg ttccgggtg ttgaagacg 
      ctgctggcg cctgatgac gtgacgagg gccgtgatg 
      gcatatcgc agggctagt agaagagaa ccacagatt 
      cgaggccta agaataacc ctttgacag ctgtgggtt 
      agaccagcg tggccttag ggaaataat caaagtgct 
      agtagttga aataaaact tgtatttta caggctgtg 
      atgtgtata aataaggga ccaattaag ttattccaa 
      aactgttat gttcgcgcg cgatttttt aaagtgtaa 
      gtggagcaa ttgattgtg ccatttttt aaccaagtt 
      gtgtactta tattttaag ccgattaca aactgcagt 
      taccaattc gttctttta tgttcctgt ttgattaga 
      tttcacttg ccagatgag ttatatgca caatttttc 
      taatctaat ttatttttg atgcgattt tttaaatat 
      ccatgactg tgactactc tatttttta atatttcta 
      aggtgctaa gtgatggca atgaaacga aatcaaact 
      ttctttctg ttgcatcat attatcata gacgaaaac 
      atttttaaa cacactttt tgtacggtt ttcaccatt 
      gccttataa acgtttttc tatagactg tgtgatgta 
      acatgatgt tgatttttt agagtataa aacagaatg 
      ttatcagat agtttattt tagatgcga tttaatgaa 
      tgaatattt ttatgtacg aataaaacg ctatcagta 
      tt
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