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Gene information
General information
TC030552 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3451485..3454155
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030552
    iBeetle screen iB_07156

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030552 RA
      MRRLNQIFA NFSEEFPIN STDLDPGGY YCDPFHSYL 
      KLLHTYYIP IIMAVGFVG NFLSCVVFL STHLKMRSS 
      SYYLAALAI ADFGFLCVV LVVHCSFNN VFEIYNADG 
      WCQFFVYLS SVCASLSVW LIVAFTVER FIAVQYPLQ 
      RPHICTVSR AKTVVISLT AVAMLSQLY LFWIAGVIN 
      NHDGKHPEC EMKPAYFEF MKVVNFVDT MATLIVPFV 
      LIVTMNTMI ARNLFLFRR RLQASSIDE YLDTDTDKT 
      ELQHSNVQS GCSNRRQGS QQSHVSQKS NPLRSSKKY 
      NNNQLERCP CIHIKMSAR NVASTRSQQ NITKMLLLI 
      SSVFIALNF PSYVTRLSV YFVFTLQQK NPPETLYCI 
      QQFAMLLYY TNFSINFLL YAMCGVTFR RCLWQLLRN 
      NLKRLASTP FHAKW
    CDS sequence
    • >TC030552 RA
      atgcgtcgc ttgaaccag atcttcgcg aacttttcg 
      gaggagttt cctatcaat tccaccgac ttagaccct 
      ggcggctac tactgcgac cctttccat tcctatctc 
      aaactgctc cacacttac tacattccc ataataatg 
      gccgtcggc ttcgtcggc aatttccta tcttgcgtg 
      gtgttcttg agcacccac ttaaaaatg cgtagttcc 
      agctactac ttggccgct ttggccatc gccgatttc 
      ggctttttg tgcgtggtt ttggtggtt cactgcagc 
      tttaataac gttttcgaa atatacaat gcggacggc 
      tggtgccag tttttcgta tacttaagc tctgtatgt 
      gcttctttg agtgtttgg cttattgtt gccttcacc 
      gtcgagaga ttcatcgct gtccagtat cctttgcaa 
      aggccgcat atttgtact gtttcgagg gcgaaaact 
      gtagttatc agtttgacg gctgtggct atgttatct 
      caactatat ctcttctgg atagctggt gttatcaat 
      aatcacgat ggaaagcat cccgaatgc gaaatgaaa 
      ccggcatac tttgaattc atgaaagtg gtcaatttt 
      gtcgatact atggcaact ttaattgtg ccctttgta 
      ctgatagtc acaatgaat acgatgatt gcgaggaat 
      cttttcttg tttcggaga agacttcag gcgagctcc 
      attgacgag tatctagac actgatacg gataaaacc 
      gaacttcag cactcaaac gtccagagc ggctgttcc 
      aataggcga caaggctcc cagcaatca cacgtctcc 
      caaaagtcc aatcctttg agatcaagt aaaaaatac 
      aacaataac caactagag agatgtcct tgcatacat 
      ataaaaatg tcagcgaga aacgttgct tcgacgcga 
      agccaacaa aacattact aaaatgctt ctcctaata 
      tcgtccgtc ttcattgca ctaaatttc ccaagttat 
      gtgactcgt ctctctgtg tattttgtc ttcaccctt 
      cagcaaaaa aatccgcct gaaacccta tactgcata 
      cagcagttc gccatgttg ctttattac accaacttc 
      agcataaac tttttactt tacgcaatg tgcggcgtg 
      acatttcgc cgttgtctc tggcaactc cttcggaat 
      aatttaaaa cggctagct agcacacct ttccacgcc 
      aagtggtaa
    mRNA sequence
    • >TC030552 RA
      cgacgtcct cacaatgcg tcgcttgaa ccagatctt 
      cgcgaactt ttcggagga gtttcctat caattccac 
      cgacttaga ccctggcgg ctactactg cgacccttt 
      ccattccta tctcaaact gctccacac ttactacat 
      tcccataat aatggccgt cggcttcgt cggcaattt 
      cctatcttg cgtggtgtt cttgagcac ccacttaaa 
      aatgcgtag ttccagcta ctacttggc cgctttggc 
      catcgccga tttcggctt tttgtgcgt ggttttggt 
      ggttcactg cagctttaa taacgtttt cgaaatata 
      caatgcgga cggctggtg ccagttttt cgtatactt 
      aagctctgt atgtgcttc tttgagtgt ttggcttat 
      tgttgcctt caccgtcga gagattcat cgctgtcca 
      gtatccttt gcaaaggcc gcatatttg tactgtttc 
      gagggcgaa aactgtagt tatcagttt gacggctgt 
      ggctatgtt atctcaact atatctctt ctggatagc 
      tggtgttat caataatca cgatggaaa gcatcccga 
      atgcgaaat gaaaccggc atactttga attcatgaa 
      agtggtcaa ttttgtcga tactatggc aactttaat 
      tgtgccctt tgtactgat agtcacaat gaatacgat 
      gattgcgag gaatctttt cttgtttcg gagaagact 
      tcaggcgag ctccattga cgagtatct agacactga 
      tacggataa aaccgaact tcagcactc aaacgtcca 
      gagcggctg ttccaatag gcgacaagg ctcccagca 
      atcacacgt ctcccaaaa gtccaatcc tttgagatc 
      aagtaaaaa atacaacaa taaccaact agagagatg 
      tccttgcat acatataaa aatgtcagc gagaaacgt 
      tgcttcgac gcgaagcca acaaaacat tactaaaat 
      gcttctcct aatatcgtc cgtcttcat tgcactaaa 
      tttcccaag ttatgtgac tcgtctctc tgtgtattt 
      tgtcttcac ccttcagca aaaaaatcc gcctgaaac 
      cctatactg catacagca gttcgccat gttgcttta 
      ttacaccaa cttcagcat aaacttttt actttacgc 
      aatgtgcgg cgtgacatt tcgccgttg tctctggca 
      actccttcg gaataattt aaaacggct agctagcac 
      acctttcca cgccaagtg gtaagaaaa tccgcctcg 
      ctttattaa actttaatt aattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY