| General information | |||
| TC007894 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 2292012..2301073 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007894 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07137 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
Fertility
mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 -
Number of animals showing the phenotype: 4
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
larval musculature pattern irregular -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007894 RA
MISTDGYNN VDQYSDGYM EQEEEWERE GLLDPAWEK QQKKTFTAW CNSHLRKAG TAIENIEED FRNGLKLML LLEVISGET LPKPDRGKM RFHKIANVN KALDFIASK GVKLVSIGA EEIVDGNLK MTLGMIWTI ILRFAIQDI SVEEMTAKE GLLLWCQRK TAPYKNVNV QNFHLSFKD GLAFCALIH RHRPDLIDY SKLSKDNPL ENLNTAFDV AEKYLDIPR MLDPDDLIN TPKPDERAI MTYVSCYYH AFQGAQQAE TAANRICKV LKVNQENER LMEEYERLA SDLLEWIRR TMPWLNSRQ TDNSLAGVQ KKLEEYRTY RRKHKPPRV EQKAKLETN FNTLQTKLR LSNRPAYMP TEGKMVSDI ANAWKGLEN AEKAFEEWL LSEMMRLER LEHLAQKFK HKADAHEEW TRGKEEMLQ SQDFRQCRL NELKALKKK HEAFESDLA AHQDRVEQI AAIAQELNS LEYHDSVSV NARCQRICD QWDRLGALT QRRRQALDE AERILEKID ILHLEFAKR AAPFNNWLD GTREDLVDI FIVHTVEEI QGLIDAHSH FKATLGEAD KEYQSIVGL VREVEAIVK QHQVPGGLQ NPYTTLTAH DLTRKWGEV RQLVPQRDA TLQGELRKQ QNNEMLRRQ FAEKANAVG PWIERQLDA VTAIGMGLH GTLEDQLHR LKEYEQGVY AYKPHIEEL EKINQAVQE SMIFENRYT QYTMETLRV GWEQLLTSI NRNINEVEN QILTRDSKG ITQQQLNEF RSSFNHFDK NRTGRLTPE EFKSCLVSL GYSIGKDRQ GDIDFQRIL AVVDPNSTG YVHFDAFLD FMTRESTDT DTAEQVIDS FRILAADKP YILPDELRR ELPPDQAEY CIQRMPPFK GPGAVPGAL DYMSFSTAL YGESDL
- >TC007894 RB
MISTDGYNN VDQYSDGYM EQEEEWERE GLLDPAWEK QQKKTFTAW CNSHLRKAG TAIENIEED FRNGLKLML LLEVISGET LPKPDRGKM RFHKIANVN KALDFIASK GVKLVSIGA EEIVDGNLK MTLGMIWTI ILRFAIQDI SVEEMTAKE GLLLWCQRK TAPYKNVNV QNFHLSFKD GLAFCALIH RHRPDLIDY SKLSKDNPL ENLNTAFDV AEKYLDIPR MLDPDDLQN TAMPDERAV MTYVSSYYH CFSGAQKAE TAANRICKV LKVNQENER LMEEYERLA SDLLEWIRR TMPWLNSRQ TDNSLAGVQ KKLEEYRTY RRKHKPPRV EQKAKLETN FNTLQTKLR LSNRPAYMP TEGKMVSDI ANAWKGLEN AEKAFEEWL LSEMMRLER LEHLAQKFK HKADAHEEW TRGKEEMLQ SQDFRQCRL NELKALKKK HEAFESDLA AHQDRVEQI AAIAQELNS LEYHDSVSV NARCQRICD QWDRLGALT QRRRQALDE AERILEKID ILHLEFAKR AAPFNNWLD GTREDLVDI FIVHTVEEI QGLIDAHSH FKATLGEAD KEYQSIVGL VREVEAIVK QHQVPGGLQ NPYTTLTAH DLTRKWGEV RQLVPQRDA TLQGELRKQ QNNEMLRRQ FAEKANAVG PWIERQLDA VTAIGMGLH GTLEDQLHR LKEYEQGVY AYKPHIEEL EKINQAVQE SMIFENRYT QYTMETLRV GWEQLLTSI NRNINEVEN QILTRDSKG ITQQQLNEF RSSFNHFDK NRTGRLTPE EFKSCLVSL GYSIGKDRQ GDIDFQRIL AVVDPNSTG YVHFDAFLD FMTRESTDT DTAEQVIDS FRILAADKP YILPDELRR ELPPDQAEY CIQRMPPFK GPGAVPGAL DYMSFSTAL YGESDL
- >TC007894 RC
MISTDGYNN VDQYSDGYM EQEEEWERE GLLDPAWEK QQKKTFTAW CNSHLRKAG TAIENIEED FRNGLKLML LLEVISGET LPKPDRGKM RFHKIANVN KALDFIASK GVKLVSIGA EEIVDGNLK MTLGMIWTI ILRFAIQDI SVEEMTAKE GLLLWCQRK TAPYKNVNV QNFHLSFKD GLAFCALIH RHRPDLIDY SKLSKDNPL ENLNTAFDV AEKYLDIPR MLDPDDLIN TPKPDERAI MTYVSCYYH AFQGAQQVS IVRKYLQNT AMPDERAVM TYVSSYYHC FSGAQKAET AANRICKVL KVNQENERL MEEYERLAS DLLEWIRRT MPWLNSRQT DNSLAGVQK KLEEYRTYR RKHKPPRVE QKAKLETNF NTLQTKLRL SNRPAYMPT EGKMVSDIA NAWKGLENA EKAFEEWLL SEMMRLERL EHLAQKFKH KADAHEEWT RGKEEMLQS QDFRQCRLN ELKALKKKH EAFESDLAA HQDRVEQIA AIAQELNSL EYHDSVSVN ARCQRICDQ WDRLGALTQ RRRQALDEA ERILEKIDI LHLEFAKRA APFNNWLDG TREDLVDIF IVHTVEEIQ GLIDAHSHF KATLGEADK EYQSIVGLV REVEAIVKQ HQVPGGLQN PYTTLTAHD LTRKWGEVR QLVPQRDAT LQGELRKQQ NNEMLRRQF AEKANAVGP WIERQLDAV TAIGMGLHG TLEDQLHRL KEYEQGVYA YKPHIEELE KINQAVQES MIFENRYTQ YTMETLRVG WEQLLTSIN RNINEVENQ ILTRDSKGI TQQQLNEFR SSFNHFDKN RTGRLTPEE FKSCLVSLG YSIGKDRQG DIDFQRILA VVDPNSTGY VHFDAFLDF MTRESTDTD TAEQVIDSF RILAADKPY ILPDELRRE LPPDQAEYC IQRMPPFKG PGAVPGALD YMSFSTALY GESDL
- >TC007894 RA
atgatcagc accgacggc tacaacaac gtcgaccag tactccgac ggctacatg gagcaggag gaggaatgg gagcgcgag ggtctcctc gacccggct tgggaaaaa cagcagaaa aagacattc acagcttgg tgcaacagc cacctgcgg aaggccggc accgccatc gaaaacatc gaagaagac ttccgcaac ggcctcaaa ctcatgctc cttctggag gtgatctcc ggtgagacc ttgccaaag cccgaccgc ggcaagatg cgtttccac aagattgcc aacgtcaac aaagcactg gacttcatc gccagcaaa ggcgtcaaa ctcgtatcg atcggtgcg gaagaaatc gtcgacggt aatctgaaa atgacgcta ggtatgatc tggaccatc atccttcga ttcgccatc caagacatc tccgtggaa gagatgacg gcgaaagaa gggctgctc ttgtggtgc cagcgcaag acagcgcct tacaaaaac gtcaacgtc caaaacttc catctgtcg ttcaaggac gggctggcc ttctgtgcg ctgatccac cgccatcga ccagatctc atcgattac agtaaactg tcgaaagat aatccattg gagaacctc aacactgcc ttcgacgta gccgagaag tatctcgac attcccagg atgttggac cctgacgat ttaatcaac acgccaaaa cccgacgag cgtgccatc atgacctac gtgtcatgt tattatcac gcgttccaa ggggcccaa caggccgag actgccgcc aacagaatc tgcaaggtc ctgaaagtc aaccaagag aacgagcgt ttgatggaa gagtacgaa cgtttggcg agtgatctc ttggaatgg atccgtcgc accatgcca tggctgaac tcccgtcaa acggacaac tccctcgcc ggcgtgcaa aagaaactg gaagaatac cgcacttac agacgcaaa cacaagccg cctcgcgtg gagcaaaaa gccaaactc gagaccaac ttcaacact ttacagacg aaactccgt ctctcaaat cggccggct tacatgccc acagaaggc aaaatggtt tcggacatt gccaacgct tggaaaggt ttggaaaac gcggagaaa gccttcgag gagtggctc ctctccgag atgatgcgt ctggaacgt ctggagcac ctcgcccag aagttcaag cacaaggcg gacgctcac gaggaatgg actcgcggc aaggaagaa atgctccaa agccaggac ttcagacaa tgtcgtctc aacgagctc aaagctttg aagaagaaa cacgaagcc ttcgaatcg gatttggcg gctcatcag gaccgagtc gagcaaatc gcagctatc gctcaagaa ctgaactct ttggagtat cacgatagc gtgagcgtg aacgcacgc tgccagcgc atttgcgac cagtgggac aggttgggc gcgttgacg cagcgccgc cgacaggcg ctggacgag gctgagagg atcctggag aagattgat attctccat ttggagttt gccaaaagg gccgcgccg tttaataat tggctggat gggacgagg gaggatttg gtcgatatt ttcatcgtg catacggtt gaggaaatt caaggactc attgacgct cattctcac tttaaagct actctaggt gaagccgat aaggaatac caaagtatc gtaggactt gttagggaa gttgaggcc attgttaag caacaccaa gtacccgga gggttacag aatccttac actacactc acggcccat gacttgacc cgcaaatgg ggcgaagtc cggcaactg gtgccccaa cgcgacgcc accctgcaa ggcgagctc cgcaagcag caaaacaac gaaatgttg cgtcggcaa ttcgcggaa aaagccaac gctgttggt ccctggatc gagcgtcaa ctcgacgct gtcaccgcc atcggaatg ggtctccac ggcaccctc gaagaccaa ctgcaccgt ctcaaagaa tacgaacag ggcgtctac gcctacaag ccacatatc gaagagctg gagaagatt aaccaagcg gtacaggaa agcatgatc tttgagaat cgctacacg cagtacacc atggagacg ctccgtgtt ggctgggaa caacttctt acgtcgatc aaccgtaat attaacgaa gtggagaac cagattttg acccgcgac tcgaaagga atcacccaa cagcagttg aatgagttt aggtcgtcg tttaatcac tttgataag aacaggact gggagactc acaccggag gagttcaag tcgtgtttg gtgtcgttg gggtactcg attgggaag gatcggcaa ggagacatc gatttccag aggattttg gccgtggtt gaccccaac agcaccgga tatgtgcac ttcgacgcg ttcttagac ttcatgacg agggaaagc acagacacc gacacggcc gagcaagtt atcgacagt ttccgcatt ttggccgcc gataagccg tacattttg ccagacgag ctgagacgg gagcttccc cccgaccaa gcggagtat tgcatccag cgcatgccc ccattcaag ggtccagga gccgtgcca ggagctttg gactacatg tccttctcg acggctttg tacggagaa tccgatctt taa
- >TC007894 RB
atgatcagc accgacggc tacaacaac gtcgaccag tactccgac ggctacatg gagcaggag gaggaatgg gagcgcgag ggtctcctc gacccggct tgggaaaaa cagcagaaa aagacattc acagcttgg tgcaacagc cacctgcgg aaggccggc accgccatc gaaaacatc gaagaagac ttccgcaac ggcctcaaa ctcatgctc cttctggag gtgatctcc ggtgagacc ttgccaaag cccgaccgc ggcaagatg cgtttccac aagattgcc aacgtcaac aaagcactg gacttcatc gccagcaaa ggcgtcaaa ctcgtatcg atcggtgcg gaagaaatc gtcgacggt aatctgaaa atgacgcta ggtatgatc tggaccatc atccttcga ttcgccatc caagacatc tccgtggaa gagatgacg gcgaaagaa gggctgctc ttgtggtgc cagcgcaag acagcgcct tacaaaaac gtcaacgtc caaaacttc catctgtcg ttcaaggac gggctggcc ttctgtgcg ctgatccac cgccatcga ccagatctc atcgattac agtaaactg tcgaaagat aatccattg gagaacctc aacactgcc ttcgacgta gccgagaag tatctcgac attcccagg atgttggac cctgacgat ttacagaac accgctatg ccggacgaa agggcggtc atgacatac gtatcgtcc tactaccat tgcttctca ggtgcacaa aaggccgag actgccgcc aacagaatc tgcaaggtc ctgaaagtc aaccaagag aacgagcgt ttgatggaa gagtacgaa cgtttggcg agtgatctc ttggaatgg atccgtcgc accatgcca tggctgaac tcccgtcaa acggacaac tccctcgcc ggcgtgcaa aagaaactg gaagaatac cgcacttac agacgcaaa cacaagccg cctcgcgtg gagcaaaaa gccaaactc gagaccaac ttcaacact ttacagacg aaactccgt ctctcaaat cggccggct tacatgccc acagaaggc aaaatggtt tcggacatt gccaacgct tggaaaggt ttggaaaac gcggagaaa gccttcgag gagtggctc ctctccgag atgatgcgt ctggaacgt ctggagcac ctcgcccag aagttcaag cacaaggcg gacgctcac gaggaatgg actcgcggc aaggaagaa atgctccaa agccaggac ttcagacaa tgtcgtctc aacgagctc aaagctttg aagaagaaa cacgaagcc ttcgaatcg gatttggcg gctcatcag gaccgagtc gagcaaatc gcagctatc gctcaagaa ctgaactct ttggagtat cacgatagc gtgagcgtg aacgcacgc tgccagcgc atttgcgac cagtgggac aggttgggc gcgttgacg cagcgccgc cgacaggcg ctggacgag gctgagagg atcctggag aagattgat attctccat ttggagttt gccaaaagg gccgcgccg tttaataat tggctggat gggacgagg gaggatttg gtcgatatt ttcatcgtg catacggtt gaggaaatt caaggactc attgacgct cattctcac tttaaagct actctaggt gaagccgat aaggaatac caaagtatc gtaggactt gttagggaa gttgaggcc attgttaag caacaccaa gtacccgga gggttacag aatccttac actacactc acggcccat gacttgacc cgcaaatgg ggcgaagtc cggcaactg gtgccccaa cgcgacgcc accctgcaa ggcgagctc cgcaagcag caaaacaac gaaatgttg cgtcggcaa ttcgcggaa aaagccaac gctgttggt ccctggatc gagcgtcaa ctcgacgct gtcaccgcc atcggaatg ggtctccac ggcaccctc gaagaccaa ctgcaccgt ctcaaagaa tacgaacag ggcgtctac gcctacaag ccacatatc gaagagctg gagaagatt aaccaagcg gtacaggaa agcatgatc tttgagaat cgctacacg cagtacacc atggagacg ctccgtgtt ggctgggaa caacttctt acgtcgatc aaccgtaat attaacgaa gtggagaac cagattttg acccgcgac tcgaaagga atcacccaa cagcagttg aatgagttt aggtcgtcg tttaatcac tttgataag aacaggact gggagactc acaccggag gagttcaag tcgtgtttg gtgtcgttg gggtactcg attgggaag gatcggcaa ggagacatc gatttccag aggattttg gccgtggtt gaccccaac agcaccgga tatgtgcac ttcgacgcg ttcttagac ttcatgacg agggaaagc acagacacc gacacggcc gagcaagtt atcgacagt ttccgcatt ttggccgcc gataagccg tacattttg ccagacgag ctgagacgg gagcttccc cccgaccaa gcggagtat tgcatccag cgcatgccc ccattcaag ggtccagga gccgtgcca ggagctttg gactacatg tccttctcg acggctttg tacggagaa tccgatctt taa
- >TC007894 RC
atgatcagc accgacggc tacaacaac gtcgaccag tactccgac ggctacatg gagcaggag gaggaatgg gagcgcgag ggtctcctc gacccggct tgggaaaaa cagcagaaa aagacattc acagcttgg tgcaacagc cacctgcgg aaggccggc accgccatc gaaaacatc gaagaagac ttccgcaac ggcctcaaa ctcatgctc cttctggag gtgatctcc ggtgagacc ttgccaaag cccgaccgc ggcaagatg cgtttccac aagattgcc aacgtcaac aaagcactg gacttcatc gccagcaaa ggcgtcaaa ctcgtatcg atcggtgcg gaagaaatc gtcgacggt aatctgaaa atgacgcta ggtatgatc tggaccatc atccttcga ttcgccatc caagacatc tccgtggaa gagatgacg gcgaaagaa gggctgctc ttgtggtgc cagcgcaag acagcgcct tacaaaaac gtcaacgtc caaaacttc catctgtcg ttcaaggac gggctggcc ttctgtgcg ctgatccac cgccatcga ccagatctc atcgattac agtaaactg tcgaaagat aatccattg gagaacctc aacactgcc ttcgacgta gccgagaag tatctcgac attcccagg atgttggac cctgacgat ttaatcaac acgccaaaa cccgacgag cgtgccatc atgacctac gtgtcatgt tattatcac gcgttccaa ggggcccaa caggtatcg attgtacga aagtattta cagaacacc gctatgccg gacgaaagg gcggtcatg acatacgta tcgtcctac taccattgc ttctcaggt gcacaaaag gccgagact gccgccaac agaatctgc aaggtcctg aaagtcaac caagagaac gagcgtttg atggaagag tacgaacgt ttggcgagt gatctcttg gaatggatc cgtcgcacc atgccatgg ctgaactcc cgtcaaacg gacaactcc ctcgccggc gtgcaaaag aaactggaa gaataccgc acttacaga cgcaaacac aagccgcct cgcgtggag caaaaagcc aaactcgag accaacttc aacacttta cagacgaaa ctccgtctc tcaaatcgg ccggcttac atgcccaca gaaggcaaa atggtttcg gacattgcc aacgcttgg aaaggtttg gaaaacgcg gagaaagcc ttcgaggag tggctcctc tccgagatg atgcgtctg gaacgtctg gagcacctc gcccagaag ttcaagcac aaggcggac gctcacgag gaatggact cgcggcaag gaagaaatg ctccaaagc caggacttc agacaatgt cgtctcaac gagctcaaa gctttgaag aagaaacac gaagccttc gaatcggat ttggcggct catcaggac cgagtcgag caaatcgca gctatcgct caagaactg aactctttg gagtatcac gatagcgtg agcgtgaac gcacgctgc cagcgcatt tgcgaccag tgggacagg ttgggcgcg ttgacgcag cgccgccga caggcgctg gacgaggct gagaggatc ctggagaag attgatatt ctccatttg gagtttgcc aaaagggcc gcgccgttt aataattgg ctggatggg acgagggag gatttggtc gatattttc atcgtgcat acggttgag gaaattcaa ggactcatt gacgctcat tctcacttt aaagctact ctaggtgaa gccgataag gaataccaa agtatcgta ggacttgtt agggaagtt gaggccatt gttaagcaa caccaagta cccggaggg ttacagaat ccttacact acactcacg gcccatgac ttgacccgc aaatggggc gaagtccgg caactggtg ccccaacgc gacgccacc ctgcaaggc gagctccgc aagcagcaa aacaacgaa atgttgcgt cggcaattc gcggaaaaa gccaacgct gttggtccc tggatcgag cgtcaactc gacgctgtc accgccatc ggaatgggt ctccacggc accctcgaa gaccaactg caccgtctc aaagaatac gaacagggc gtctacgcc tacaagcca catatcgaa gagctggag aagattaac caagcggta caggaaagc atgatcttt gagaatcgc tacacgcag tacaccatg gagacgctc cgtgttggc tgggaacaa cttcttacg tcgatcaac cgtaatatt aacgaagtg gagaaccag attttgacc cgcgactcg aaaggaatc acccaacag cagttgaat gagtttagg tcgtcgttt aatcacttt gataagaac aggactggg agactcaca ccggaggag ttcaagtcg tgtttggtg tcgttgggg tactcgatt gggaaggat cggcaagga gacatcgat ttccagagg attttggcc gtggttgac cccaacagc accggatat gtgcacttc gacgcgttc ttagacttc atgacgagg gaaagcaca gacaccgac acggccgag caagttatc gacagtttc cgcattttg gccgccgat aagccgtac attttgcca gacgagctg agacgggag cttcccccc gaccaagcg gagtattgc atccagcgc atgccccca ttcaagggt ccaggagcc gtgccagga gctttggac tacatgtcc ttctcgacg gctttgtac ggagaatcc gatctttaa
- >TC007894 RA
gacgtcacc ttttttgaa aaattcctg tgaaatttt ggcggcaaa ttcaaaatc gcctgtatg tttacgttt ggtccgctt ctttgcgat aacctccta ctagaagct ggtgtggag tttgcgcca acagcccga caagcaact ggcaagcag aaagcagtc gggcacggg ctagtgggg taaagtgcg tgtcaggtc gtgtgcttt tgcacgact gaattccat ctgccgacc gaacattta aacgaactt agaattcag ttactgaaa agcgtgcat ttcgacgaa aatgatcag caccgacgg ctacaacaa cgtcgacca gtactccga cggctacat ggagcagga ggaggaatg ggagcgcga gggtctcct cgacccggc ttgggaaaa acagcagaa aaagacatt cacagcttg gtgcaacag ccacctgcg gaaggccgg caccgccat cgaaaacat cgaagaaga cttccgcaa cggcctcaa actcatgct ccttctgga ggtgatctc cggtgagac cttgccaaa gcccgaccg cggcaagat gcgtttcca caagattgc caacgtcaa caaagcact ggacttcat cgccagcaa aggcgtcaa actcgtatc gatcggtgc ggaagaaat cgtcgacgg taatctgaa aatgacgct aggtatgat ctggaccat catccttcg attcgccat ccaagacat ctccgtgga agagatgac ggcgaaaga agggctgct cttgtggtg ccagcgcaa gacagcgcc ttacaaaaa cgtcaacgt ccaaaactt ccatctgtc gttcaagga cgggctggc cttctgtgc gctgatcca ccgccatcg accagatct catcgatta cagtaaact gtcgaaaga taatccatt ggagaacct caacactgc cttcgacgt agccgagaa gtatctcga cattcccag gatgttgga ccctgacga tttaatcaa cacgccaaa acccgacga gcgtgccat catgaccta cgtgtcatg ttattatca cgcgttcca aggggccca acaggccga gactgccgc caacagaat ctgcaaggt cctgaaagt caaccaaga gaacgagcg tttgatgga agagtacga acgtttggc gagtgatct cttggaatg gatccgtcg caccatgcc atggctgaa ctcccgtca aacggacaa ctccctcgc cggcgtgca aaagaaact ggaagaata ccgcactta cagacgcaa acacaagcc gcctcgcgt ggagcaaaa agccaaact cgagaccaa cttcaacac tttacagac gaaactccg tctctcaaa tcggccggc ttacatgcc cacagaagg caaaatggt ttcggacat tgccaacgc ttggaaagg tttggaaaa cgcggagaa agccttcga ggagtggct cctctccga gatgatgcg tctggaacg tctggagca cctcgccca gaagttcaa gcacaaggc ggacgctca cgaggaatg gactcgcgg caaggaaga aatgctcca aagccagga cttcagaca atgtcgtct caacgagct caaagcttt gaagaagaa acacgaagc cttcgaatc ggatttggc ggctcatca ggaccgagt cgagcaaat cgcagctat cgctcaaga actgaactc tttggagta tcacgatag cgtgagcgt gaacgcacg ctgccagcg catttgcga ccagtggga caggttggg cgcgttgac gcagcgccg ccgacaggc gctggacga ggctgagag gatcctgga gaagattga tattctcca tttggagtt tgccaaaag ggccgcgcc gtttaataa ttggctgga tgggacgag ggaggattt ggtcgatat tttcatcgt gcatacggt tgaggaaat tcaaggact cattgacgc tcattctca ctttaaagc tactctagg tgaagccga taaggaata ccaaagtat cgtaggact tgttaggga agttgaggc cattgttaa gcaacacca agtacccgg agggttaca gaatcctta cactacact cacggccca tgacttgac ccgcaaatg gggcgaagt ccggcaact ggtgcccca acgcgacgc caccctgca aggcgagct ccgcaagca gcaaaacaa cgaaatgtt gcgtcggca attcgcgga aaaagccaa cgctgttgg tccctggat cgagcgtca actcgacgc tgtcaccgc catcggaat gggtctcca cggcaccct cgaagacca actgcaccg tctcaaaga atacgaaca gggcgtcta cgcctacaa gccacatat cgaagagct ggagaagat taaccaagc ggtacagga aagcatgat ctttgagaa tcgctacac gcagtacac catggagac gctccgtgt tggctggga acaacttct tacgtcgat caaccgtaa tattaacga agtggagaa ccagatttt gacccgcga ctcgaaagg aatcaccca acagcagtt gaatgagtt taggtcgtc gtttaatca ctttgataa gaacaggac tgggagact cacaccgga ggagttcaa gtcgtgttt ggtgtcgtt ggggtactc gattgggaa ggatcggca aggagacat cgatttcca gaggatttt ggccgtggt tgaccccaa cagcaccgg atatgtgca cttcgacgc gttcttaga cttcatgac gagggaaag cacagacac cgacacggc cgagcaagt tatcgacag tttccgcat tttggccgc cgataagcc gtacatttt gccagacga gctgagacg ggagcttcc ccccgacca agcggagta ttgcatcca gcgcatgcc cccattcaa gggtccagg agccgtgcc aggagcttt ggactacat gtccttctc gacggcttt gtacggaga atccgatct ttaaaaata aagttcatg ccttacccc accccttat ttatctacg acaggccga gaaatcggt ctgaaaccg agcatcgat acaattttt ttttcgctt ggtatatta cgatcattt taattttta acggaaagt gtttgccgt ttctcaatc atttacgta ctttttttg caagtgctc agatttatt tatttattt aaggcttca gttgtacag tttttggat aaacgctct ttattacac acgtaagac taagaaata aggagcttt tattatgtt tttggttgg agttaattt atggttatt gaaatgtaa aaactttat atttttttg cccaaagta tactaacat tacgtatat cgaataaat tttatttga tttaacgcg tttattttt ttctttttt ttccagata taattgtag gtttttgcg cagctattg ctgcggcga ggtatgtag tactatatg ctcagaact ttatatttc gtaagttac aggtatgtg aatatatgt aattttaaa ac
- >TC007894 RB
gacgtcacc ttttttgaa aaattcctg tgaaatttt ggcggcaaa ttcaaaatc gcctgtatg tttacgttt ggtccgctt ctttgcgat aacctccta ctagaagct ggtgtggag tttgcgcca acagcccga caagcaact ggcaagcag aaagcagtc gggcacggg ctagtgggg taaagtgcg tgtcaggtc gtgtgcttt tgcacgact gaattccat ctgccgacc gaacattta aacgaactt agaattcag ttactgaaa agcgtgcat ttcgacgaa aatgatcag caccgacgg ctacaacaa cgtcgacca gtactccga cggctacat ggagcagga ggaggaatg ggagcgcga gggtctcct cgacccggc ttgggaaaa acagcagaa aaagacatt cacagcttg gtgcaacag ccacctgcg gaaggccgg caccgccat cgaaaacat cgaagaaga cttccgcaa cggcctcaa actcatgct ccttctgga ggtgatctc cggtgagac cttgccaaa gcccgaccg cggcaagat gcgtttcca caagattgc caacgtcaa caaagcact ggacttcat cgccagcaa aggcgtcaa actcgtatc gatcggtgc ggaagaaat cgtcgacgg taatctgaa aatgacgct aggtatgat ctggaccat catccttcg attcgccat ccaagacat ctccgtgga agagatgac ggcgaaaga agggctgct cttgtggtg ccagcgcaa gacagcgcc ttacaaaaa cgtcaacgt ccaaaactt ccatctgtc gttcaagga cgggctggc cttctgtgc gctgatcca ccgccatcg accagatct catcgatta cagtaaact gtcgaaaga taatccatt ggagaacct caacactgc cttcgacgt agccgagaa gtatctcga cattcccag gatgttgga ccctgacga tttacagaa caccgctat gccggacga aagggcggt catgacata cgtatcgtc ctactacca ttgcttctc aggtgcaca aaaggccga gactgccgc caacagaat ctgcaaggt cctgaaagt caaccaaga gaacgagcg tttgatgga agagtacga acgtttggc gagtgatct cttggaatg gatccgtcg caccatgcc atggctgaa ctcccgtca aacggacaa ctccctcgc cggcgtgca aaagaaact ggaagaata ccgcactta cagacgcaa acacaagcc gcctcgcgt ggagcaaaa agccaaact cgagaccaa cttcaacac tttacagac gaaactccg tctctcaaa tcggccggc ttacatgcc cacagaagg caaaatggt ttcggacat tgccaacgc ttggaaagg tttggaaaa cgcggagaa agccttcga ggagtggct cctctccga gatgatgcg tctggaacg tctggagca cctcgccca gaagttcaa gcacaaggc ggacgctca cgaggaatg gactcgcgg caaggaaga aatgctcca aagccagga cttcagaca atgtcgtct caacgagct caaagcttt gaagaagaa acacgaagc cttcgaatc ggatttggc ggctcatca ggaccgagt cgagcaaat cgcagctat cgctcaaga actgaactc tttggagta tcacgatag cgtgagcgt gaacgcacg ctgccagcg catttgcga ccagtggga caggttggg cgcgttgac gcagcgccg ccgacaggc gctggacga ggctgagag gatcctgga gaagattga tattctcca tttggagtt tgccaaaag ggccgcgcc gtttaataa ttggctgga tgggacgag ggaggattt ggtcgatat tttcatcgt gcatacggt tgaggaaat tcaaggact cattgacgc tcattctca ctttaaagc tactctagg tgaagccga taaggaata ccaaagtat cgtaggact tgttaggga agttgaggc cattgttaa gcaacacca agtacccgg agggttaca gaatcctta cactacact cacggccca tgacttgac ccgcaaatg gggcgaagt ccggcaact ggtgcccca acgcgacgc caccctgca aggcgagct ccgcaagca gcaaaacaa cgaaatgtt gcgtcggca attcgcgga aaaagccaa cgctgttgg tccctggat cgagcgtca actcgacgc tgtcaccgc catcggaat gggtctcca cggcaccct cgaagacca actgcaccg tctcaaaga atacgaaca gggcgtcta cgcctacaa gccacatat cgaagagct ggagaagat taaccaagc ggtacagga aagcatgat ctttgagaa tcgctacac gcagtacac catggagac gctccgtgt tggctggga acaacttct tacgtcgat caaccgtaa tattaacga agtggagaa ccagatttt gacccgcga ctcgaaagg aatcaccca acagcagtt gaatgagtt taggtcgtc gtttaatca ctttgataa gaacaggac tgggagact cacaccgga ggagttcaa gtcgtgttt ggtgtcgtt ggggtactc gattgggaa ggatcggca aggagacat cgatttcca gaggatttt ggccgtggt tgaccccaa cagcaccgg atatgtgca cttcgacgc gttcttaga cttcatgac gagggaaag cacagacac cgacacggc cgagcaagt tatcgacag tttccgcat tttggccgc cgataagcc gtacatttt gccagacga gctgagacg ggagcttcc ccccgacca agcggagta ttgcatcca gcgcatgcc cccattcaa gggtccagg agccgtgcc aggagcttt ggactacat gtccttctc gacggcttt gtacggaga atccgatct ttaaaaata aagttcatg ccttacccc accccttat ttatctacg acaggccga gaaatcggt ctgaaaccg agcatcgat acaattttt ttttcgctt ggtatatta cgatcattt taattttta acggaaagt gtttgccgt ttctcaatc atttacgta ctttttttg caagtgctc agatttatt tatttattt aaggcttca gttgtacag tttttggat aaacgctct ttattacac acgtaagac taagaaata aggagcttt tattatgtt tttggttgg agttaattt atggttatt gaaatgtaa aaactttat atttttttg cccaaagta tactaacat tacgtatat cgaataaat tttatttga tttaacgcg tttattttt ttctttttt ttccagata taattgtag gtttttgcg cagctattg ctgcggcga ggtatgtag tactatatg ctcagaact ttatatttc gtaagttac aggtatgtg aatatatgt aattttaaa ac
- >TC007894 RC
gacgtcacc ttttttgaa aaattcctg tgaaatttt ggcggcaaa ttcaaaatc gcctgtatg tttacgttt ggtccgctt ctttgcgat aacctccta ctagaagct ggtgtggag tttgcgcca acagcccga caagcaact ggcaagcag aaagcagtc gggcacggg ctagtgggg taaagtgcg tgtcaggtc gtgtgcttt tgcacgact gaattccat ctgccgacc gaacattta aacgaactt agaattcag ttactgaaa agcgtgcat ttcgacgaa aatgatcag caccgacgg ctacaacaa cgtcgacca gtactccga cggctacat ggagcagga ggaggaatg ggagcgcga gggtctcct cgacccggc ttgggaaaa acagcagaa aaagacatt cacagcttg gtgcaacag ccacctgcg gaaggccgg caccgccat cgaaaacat cgaagaaga cttccgcaa cggcctcaa actcatgct ccttctgga ggtgatctc cggtgagac cttgccaaa gcccgaccg cggcaagat gcgtttcca caagattgc caacgtcaa caaagcact ggacttcat cgccagcaa aggcgtcaa actcgtatc gatcggtgc ggaagaaat cgtcgacgg taatctgaa aatgacgct aggtatgat ctggaccat catccttcg attcgccat ccaagacat ctccgtgga agagatgac ggcgaaaga agggctgct cttgtggtg ccagcgcaa gacagcgcc ttacaaaaa cgtcaacgt ccaaaactt ccatctgtc gttcaagga cgggctggc cttctgtgc gctgatcca ccgccatcg accagatct catcgatta cagtaaact gtcgaaaga taatccatt ggagaacct caacactgc cttcgacgt agccgagaa gtatctcga cattcccag gatgttgga ccctgacga tttaatcaa cacgccaaa acccgacga gcgtgccat catgaccta cgtgtcatg ttattatca cgcgttcca aggggccca acaggtatc gattgtacg aaagtattt acagaacac cgctatgcc ggacgaaag ggcggtcat gacatacgt atcgtccta ctaccattg cttctcagg tgcacaaaa ggccgagac tgccgccaa cagaatctg caaggtcct gaaagtcaa ccaagagaa cgagcgttt gatggaaga gtacgaacg tttggcgag tgatctctt ggaatggat ccgtcgcac catgccatg gctgaactc ccgtcaaac ggacaactc cctcgccgg cgtgcaaaa gaaactgga agaataccg cacttacag acgcaaaca caagccgcc tcgcgtgga gcaaaaagc caaactcga gaccaactt caacacttt acagacgaa actccgtct ctcaaatcg gccggctta catgcccac agaaggcaa aatggtttc ggacattgc caacgcttg gaaaggttt ggaaaacgc ggagaaagc cttcgagga gtggctcct ctccgagat gatgcgtct ggaacgtct ggagcacct cgcccagaa gttcaagca caaggcgga cgctcacga ggaatggac tcgcggcaa ggaagaaat gctccaaag ccaggactt cagacaatg tcgtctcaa cgagctcaa agctttgaa gaagaaaca cgaagcctt cgaatcgga tttggcggc tcatcagga ccgagtcga gcaaatcgc agctatcgc tcaagaact gaactcttt ggagtatca cgatagcgt gagcgtgaa cgcacgctg ccagcgcat ttgcgacca gtgggacag gttgggcgc gttgacgca gcgccgccg acaggcgct ggacgaggc tgagaggat cctggagaa gattgatat tctccattt ggagtttgc caaaagggc cgcgccgtt taataattg gctggatgg gacgaggga ggatttggt cgatatttt catcgtgca tacggttga ggaaattca aggactcat tgacgctca ttctcactt taaagctac tctaggtga agccgataa ggaatacca aagtatcgt aggacttgt tagggaagt tgaggccat tgttaagca acaccaagt acccggagg gttacagaa tccttacac tacactcac ggcccatga cttgacccg caaatgggg cgaagtccg gcaactggt gccccaacg cgacgccac cctgcaagg cgagctccg caagcagca aaacaacga aatgttgcg tcggcaatt cgcggaaaa agccaacgc tgttggtcc ctggatcga gcgtcaact cgacgctgt caccgccat cggaatggg tctccacgg caccctcga agaccaact gcaccgtct caaagaata cgaacaggg cgtctacgc ctacaagcc acatatcga agagctgga gaagattaa ccaagcggt acaggaaag catgatctt tgagaatcg ctacacgca gtacaccat ggagacgct ccgtgttgg ctgggaaca acttcttac gtcgatcaa ccgtaatat taacgaagt ggagaacca gattttgac ccgcgactc gaaaggaat cacccaaca gcagttgaa tgagtttag gtcgtcgtt taatcactt tgataagaa caggactgg gagactcac accggagga gttcaagtc gtgtttggt gtcgttggg gtactcgat tgggaagga tcggcaagg agacatcga tttccagag gattttggc cgtggttga ccccaacag caccggata tgtgcactt cgacgcgtt cttagactt catgacgag ggaaagcac agacaccga cacggccga gcaagttat cgacagttt ccgcatttt ggccgccga taagccgta cattttgcc agacgagct gagacggga gcttccccc cgaccaagc ggagtattg catccagcg catgccccc attcaaggg tccaggagc cgtgccagg agctttgga ctacatgtc cttctcgac ggctttgta cggagaatc cgatcttta aaaataaag ttcatgcct taccccacc ccttattta tctacgaca ggccgagaa atcggtctg aaaccgagc atcgataca atttttttt tcgcttggt atattacga tcattttaa tttttaacg gaaagtgtt tgccgtttc tcaatcatt tacgtactt tttttgcaa gtgctcaga tttatttat ttatttaag gcttcagtt gtacagttt ttggataaa cgctcttta ttacacacg taagactaa gaaataagg agcttttat tatgttttt ggttggagt taatttatg gttattgaa atgtaaaaa ctttatatt tttttgccc aaagtatac taacattac gtatatcga ataaatttt atttgattt aacgcgttt atttttttc ttttttttc cagatataa ttgtaggtt tttgcgcag ctattgctg cggcgaggt atgtagtac tatatgctc agaacttta tatttcgta agttacagg tatgtgaat atatgtaat tttaaaac
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