Status
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Gene information
General information
TC007678 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 992774..997171
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007678
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07125

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007678 RA
      MRVVALISG GKDSCFNMM QCIAAGHEI VALANISPH 
      NKSELDSYM YQSVGHEAI EFIAAAMDL PLYREATRG 
      VSTQQGKIY EPSPDDEVE DLFRVLGQV KGDLEVEGV 
      SVGAILSDY QRVRVENVC VRLGLVPLA YLWQRDQKE 
      LLDEMIKCE VDAVIIKVA ALGLDPGKH LGRTLSSMQ 
      PHLLAMSEK YGLNVCGEG GEYETLTLD CPLFRSRLV 
      IDESDVVLH SSDPIAPVG YLQLKKISL ETKLPLLDL 
      QDRLSGLPL KNSDGYVTD YGVEASDFS DDESVNSLE 
      NATVTLETI EQEPMGVKS RDGWLCIAG IQGRSSDFR 
      QAMEEAAAK LKSILGQHD QTVQDVCSL VMFISDMSR 
      YAELNQVYC ETFNHVNPP SRACVEVPS CFPVILTAL 
      SWKEPHNQA GDFPLERHT MHVQSRSHW APANIGPYS 
      QAVRVGELI HLAGQIGMI PGSLEMVKG GIKAQCQLA 
      LRHVGRLLK AVDSNVNLR DVVQGICYV TDVSYVEHA 
      RKLWEEKTN NAIVDYVVV SQLPKSALV EWHVWAHRH 
      NNQFEYEET GKCFQGYSI SIYRRWNYE NDIAAVVCY 
      VHNTEETEL SEDIFIETV DYVIQKLNQ GHENDATSV 
      YNLNIFYPR MKIRTLNVD YLQKLAENI ALVYTLVPV 
      TYVKSEHTY LSICGVRNQ
    CDS sequence
    • >TC007678 RA
      atgagggtg gttgccctg atcagtggg ggcaaagac 
      agctgcttc aacatgatg cagtgcatc gcagcgggg 
      catgagatc gtagccctg gccaacatc agcccccat 
      aacaagtcc gagttggac agttacatg taccagagt 
      gtggggcac gaagctatc gagtttatt gcagcggcg 
      atggacttg cccctgtat cgggaggcc accaggggg 
      gtgagcacc caacagggc aaaatctac gagcccagt 
      ccggacgac gaagtggag gatttgttc cgggtgctg 
      gggcaggtg aagggggac ttggaggtg gagggggtg 
      tccgtgggg gcgatcttg tcggattac cagcgagtt 
      cgggtcgaa aatgtatgc gtaagactg gggcttgtg 
      cccttggcg tatttatgg caacgcgac caaaaggaa 
      ctacttgac gaaatgatc aaatgcgag gtggatgcg 
      gttataatc aaagttgca gctttgggg ctcgacccc 
      gggaaacac ctggggcgc actttgagt tcaatgcag 
      ccccattta ctggcaatg agcgaaaag tacgggttg 
      aatgtttgc ggcgaaggg ggcgagtac gagacactc 
      acacttgac tgcccactg ttcaggagc agattagtc 
      attgatgaa tcggacgta gttttgcat tcttctgac 
      cccatagct cccgtgggt tacctccag ttaaaaaaa 
      atcagtctt gaaaccaaa ctgccactt cttgattta 
      caagatcgc ctcagtggt ttacccttg aaaaatagt 
      gacggttat gtgaccgat tacggggtc gaagcgtcc 
      gattttagc gatgatgag tcggtgaat tcgttagaa 
      aacgccact gtcacacta gagaccatt gaacaggaa 
      ccaatgggt gtgaaaagt cgtgatggg tggctttgt 
      attgctggt attcaaggc cgatcttcc gattttcgc 
      caagctatg gaggaagca gccgccaaa ttaaagtct 
      attctgggt caacatgac caaaccgtc caagatgta 
      tgttctttg gtgatgttt atctccgat atgtcacga 
      tatgcggaa cttaatcaa gtctactgt gagactttt 
      aatcatgta aatccccca agtcgggcc tgtgttgag 
      gtgccatcg tgttttccg gttatttta acagcgttg 
      tcgtggaag gagccgcat aatcaagct ggggatttc 
      ccccttgaa agacataca atgcatgtc cagagccgg 
      tctcactgg gcccctgcc aatatcggg ccctacagt 
      caggctgta agagtaggt gaattgata cacctggca 
      ggccaaatt ggcatgatc ccaggaagt ttagaaatg 
      gtcaaaggt gggattaaa gcccagtgc caactagcc 
      ttaagacat gtgggaagg ctccttaaa gcagtcgat 
      tcaaatgtt aatcttaga gacgtcgta cagggaatt 
      tgttacgtg actgatgtt agttacgtc gaacacgct 
      cgtaaatta tgggaggaa aaaacaaat aatgccata 
      gtggactac gttgtagtg agccaattg ccaaaaagt 
      gctttagta gagtggcac gtgtgggca caccgtcac 
      aacaaccaa tttgaatat gaggagact ggaaagtgc 
      ttccaaggt tattccatc tcgatttat cgacgctgg 
      aattacgaa aacgacata gcggcagtc gtttgttac 
      gtgcataac acggaagaa accgaatta agtgaagat 
      atttttata gaaactgta gattacgtt atacagaaa 
      ctaaatcag ggccatgaa aacgacgca acttcggtc 
      tacaatctg aatattttt tatccccga atgaaaatt 
      cggactctt aacgtggac tatttgcaa aagcttgcc 
      gaaaatatt gcgctggtt tacacactg gtacctgtg 
      acgtacgtc aagagtgaa cacacctat ctgtcgatt 
      tgtggcgtc cgaaaccag taa
    mRNA sequence
    • >TC007678 RA
      tgtctctag ctactcctc cagtgcaaa gcgggataa 
      aagcagcag caaaaaata ctcggaagc gtaaaatag 
      caaaaggct tttaccacg tacatacat caaggaggg 
      gaaaggaaa ttcggggaa gaaaacgca aacacacaa 
      ctatagttt aatgagcaa aaataatat taatagtac 
      aatagtaag gtggctgtg gcgacttgg cgccgaatt 
      gcacagatc cgtagtgca aattttaca attttggtg 
      cgtgtctgt attaataat tttgcaaaa ttttaaata 
      tttagctgt atggttttg gtcgtgccg gacacagtc 
      tttgagtat tgaatctga aaaaatcct tgattaaac 
      caccgaaac gtttgaaca actcacttt ccagaataa 
      tcttaatac aactatcag agtccggag ggcaaaccc 
      attgggccg gcttagcac aagaaacga ttttaacca 
      atggattgt caatcgcca tattcacat tacaataat 
      agcacctta tactcaacg ttagcccaa aatttagca 
      acaaaatta taaatattc tgacatttt tcatcattt 
      gaagtttga atattctgg aaactttgt agcggctac 
      gttggtagc gcgtgccgt tctaacctc caattcaaa 
      agtgacctt caccatgtt attttcgct tttttcgtt 
      ccgttttat ctaatttgg tcatcatga gggtggttg 
      ccctgatca gtgggggca aagacagct gcttcaaca 
      tgatgcagt gcatcgcag cggggcatg agatcgtag 
      ccctggcca acatcagcc cccataaca agtccgagt 
      tggacagtt acatgtacc agagtgtgg ggcacgaag 
      ctatcgagt ttattgcag cggcgatgg acttgcccc 
      tgtatcggg aggccacca ggggggtga gcacccaac 
      agggcaaaa tctacgagc ccagtccgg acgacgaag 
      tggaggatt tgttccggg tgctggggc aggtgaagg 
      gggacttgg aggtggagg gggtgtccg tgggggcga 
      tcttgtcgg attaccagc gagttcggg tcgaaaatg 
      tatgcgtaa gactggggc ttgtgccct tggcgtatt 
      tatggcaac gcgaccaaa aggaactac ttgacgaaa 
      tgatcaaat gcgaggtgg atgcggtta taatcaaag 
      ttgcagctt tggggctcg accccggga aacacctgg 
      ggcgcactt tgagttcaa tgcagcccc atttactgg 
      caatgagcg aaaagtacg ggttgaatg tttgcggcg 
      aagggggcg agtacgaga cactcacac ttgactgcc 
      cactgttca ggagcagat tagtcattg atgaatcgg 
      acgtagttt tgcattctt ctgacccca tagctcccg 
      tgggttacc tccagttaa aaaaaatca gtcttgaaa 
      ccaaactgc cacttcttg atttacaag atcgcctca 
      gtggtttac ccttgaaaa atagtgacg gttatgtga 
      ccgattacg gggtcgaag cgtccgatt ttagcgatg 
      atgagtcgg tgaattcgt tagaaaacg ccactgtca 
      cactagaga ccattgaac aggaaccaa tgggtgtga 
      aaagtcgtg atgggtggc tttgtattg ctggtattc 
      aaggccgat cttccgatt ttcgccaag ctatggagg 
      aagcagccg ccaaattaa agtctattc tgggtcaac 
      atgaccaaa ccgtccaag atgtatgtt ctttggtga 
      tgtttatct ccgatatgt cacgatatg cggaactta 
      atcaagtct actgtgaga cttttaatc atgtaaatc 
      ccccaagtc gggcctgtg ttgaggtgc catcgtgtt 
      ttccggtta ttttaacag cgttgtcgt ggaaggagc 
      cgcataatc aagctgggg atttccccc ttgaaagac 
      atacaatgc atgtccaga gccggtctc actgggccc 
      ctgccaata tcgggccct acagtcagg ctgtaagag 
      taggtgaat tgatacacc tggcaggcc aaattggca 
      tgatcccag gaagtttag aaatggtca aaggtggga 
      ttaaagccc agtgccaac tagccttaa gacatgtgg 
      gaaggctcc ttaaagcag tcgattcaa atgttaatc 
      ttagagacg tcgtacagg gaatttgtt acgtgactg 
      atgttagtt acgtcgaac acgctcgta aattatggg 
      aggaaaaaa caaataatg ccatagtgg actacgttg 
      tagtgagcc aattgccaa aaagtgctt tagtagagt 
      ggcacgtgt gggcacacc gtcacaaca accaatttg 
      aatatgagg agactggaa agtgcttcc aaggttatt 
      ccatctcga tttatcgac gctggaatt acgaaaacg 
      acatagcgg cagtcgttt gttacgtgc ataacacgg 
      aagaaaccg aattaagtg aagatattt ttatagaaa 
      ctgtagatt acgttatac agaaactaa atcagggcc 
      atgaaaacg acgcaactt cggtctaca atctgaata 
      ttttttatc cccgaatga aaattcgga ctcttaacg 
      tggactatt tgcaaaagc ttgccgaaa atattgcgc 
      tggtttaca cactggtac ctgtgacgt acgtcaaga 
      gtgaacaca cctatctgt cgatttgtg gcgtccgaa 
      accagtaat tgttgtttt attttgact gttgttgtt 
      ttacaaata tacatttat ttagtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY