Status
Please wait ...
Gene information
General information
TC007799 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 676043..689839
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007799
    iBeetle screen iB_07120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007799 RA
      MKRCAKLFA VVFLVFVSV ASVIRLCFC FWSGLFESD 
      NKPDGKVDE WLERNNLSP FKDCFVDKG ISQLAQCGQ 
      LGDLPELPA SDEQRLQKA AALLQQRLV LRQWLTKYN 
      LQHYYPRLL NLEVTSLED VYWLEDTKA KSILQKDFP 
      KWSTARQSL PTRKPHLET LKADLWSEV VKSSSHQDA 
      WTWGGMLVV SVSVAGLVT LAAMTQPSL APEAKHSLL 
      QYVTGKYLL PSNCKVIFH WSDPHPVGH TVCFTVQFY 
      QRNGQPYPI CDTDNFVVD VCEGSRKMV VITELGSPT 
      DPNSANIAK VKFMVRHAG QYRITIMVS NQHVAGSPF 
      MRTFIAGPA YAQRTVVVR HCSTVVCTA NVAHLLYIE 
      PRDEYGNVC SYPSDDDPT QDYTVHITQ LGTYSKESN 
      LDNDDNNFT TALDYDWQS QRVSLQLKF LYEGCYHTT 
      IKYKGIELH NGDFDIIVL NSIDSTLVH KNVASKNHN 
      ICYEAKLIG VNNERLQKA KKVLCYVSP KQLIIKELI 
      LKFIPKRLY TFRLCPSTK FSFQTEAKT MDHPTGYFT 
      VDDGCQPKI ELISKERNV IAATFAQFL LKNMGGSET 
      FKDKQDFFY HEVRKYHQK HYHEKLSMK VNREKLLES 
      SMKATKNFS VSEWCRNFE ITFQGEQGI DWGGLRREW 
      FELICSQLF DAKNALFTS FHEGQQSLV HPNPSRPSH 
      LKLRHYEFA GKVVGKCLY ESALGGSYR QLVRARFTR 
      SFLAQVIGL RVHYKYFEQ DDPDLYLSK IKYLLENDI 
      DQIDTELYF VEEQYDTSG QLLKTVELI PNGSKIRVR 
      NATKLQYLD ALAQYRLAT SIKDEMEAF LKGLNELIP 
      DNLLSIFDE NELELLLCG TGQYSIADF KQHHVINGN 
      SAEFRRIVG WFWAAVGNF TQEEMARLL QFTTGCSQL 
      PPGGFKELT PKFQITAAP TFGNLPTAH TCFNQLCLP 
      DYDCYEHFE KSLLLAISE GTEGFGMV
    CDS sequence
    • >TC007799 RA
      atgaagcgt tgtgccaaa ctattcgct gttgtattt 
      ttggttttt gttagtgtt gctagtgtg ataagactg 
      tgtttttgc ttttggtct ggattattt gaatcggac 
      aataaaccg gatggtaaa gtcgatgaa tggctagaa 
      cggaacaat ttgtcaccg ttcaaggac tgttttgtt 
      gacaaaggc atatcccag ctggcccaa tgcggccaa 
      ttgggcgat ttgccagaa cttcccgcc tccgacgag 
      caacgtctc caaaaggca gccgccttg ttacagcaa 
      cgtctcgtc ctccgccaa tggctaaca aaatacaac 
      ctccagcac tactatcct cgactcctt aacctcgaa 
      gtgacaagc ttggaggac gtctattgg ttagaagac 
      accaaagct aaatccatc ttgcagaaa gacttcccc 
      aagtggtca actgcaaga caatccttg ccgacacgt 
      aagccgcac ttggaaaca ctcaaagcc gatctttgg 
      tccgaagtt gtcaagtca agcagccac caagacgcc 
      tggacatgg ggcggtatg ttggtcgtt tctgtgtca 
      gttgccggt ttggtaacg ttagctgca atgacccag 
      ccgtcattg gcccccgaa gctaaacat tcccttctc 
      cagtatgta accggaaaa tatctacta ccatcaaac 
      tgtaaagtg attttccat tggtcggac cctcaccct 
      gtaggtcat acggtttgt ttcactgtc cagttttat 
      cagcgcaat ggccagcct tatccgatc tgtgataca 
      gacaatttt gttgttgat gtttgcgaa ggttcacgt 
      aaaatggtg gttataacc gaacttgga tcaccgacc 
      gatccaaac agtgctaac attgccaaa gttaagttt 
      atggtacga catgcaggc cagtatcga atcacaatt 
      atggtaagc aatcagcat gtggcagga agtcctttt 
      atgaggact ttcatcgct gggccggct tatgcccaa 
      aggacggtt gttgtgagg cattgtagt acggttgtg 
      tgtacggca aatgttgca catttgttg tacattgaa 
      ccacgggat gagtatgga aatgtttgt agctatcct 
      tcagacgat gatccgaca caggattac acagtccat 
      attacacag ttaggaacc tattctaaa gaatcaaac 
      ttggacaat gacgacaat aattttacc acggcttta 
      gattacgac tggcaaagt caacgagtg agtcttcaa 
      ttgaaattt ctctacgaa ggttgttat cacaccaca 
      attaaatat aaaggaatc gagttacat aatggcgat 
      tttgacatt attgtttta aacagcatc gattcaact 
      cttgtccac aaaaatgtg gcttcgaaa aaccacaat 
      atttgctac gaagctaaa ttaattggt gtcaataat 
      gagcgactg caaaaagcg aaaaaagtc ctgtgttac 
      gtttcgccc aaacagctc atcatcaag gaactcatc 
      ctgaaattt atccccaaa cgcttgtac acgttccgg 
      ttgtgtcct tcaaccaaa ttcagtttt caaactgaa 
      gccaaaacg atggaccac cccactggt tatttcaca 
      gtggacgat ggttgccaa cctaaaatc gaattaatt 
      tcaaaagaa cgtaacgtc atagcggct acttttgcc 
      caattcctt ctcaaaaac atgggcggt tccgaaact 
      tttaaagat aaacaggac ttcttctac catgaggta 
      cgcaagtac catcagaaa cactaccat gagaagctc 
      tcgatgaaa gtcaatcga gagaaattg ctagaatcg 
      agcatgaaa gcaacgaag aatttttca gtgagcgag 
      tggtgccgt aacttcgaa atcacattt caaggagaa 
      caaggcatc gactggggc ggcttgcgc cgcgaatgg 
      ttcgagctg atttgctcg caacttttc gacgccaaa 
      aacgctcta tttaccagt ttccacgag ggccaacaa 
      tcgctcgta cacccgaat ccaagccgc ccttcgcac 
      ctaaaactg cgccactac gaattcgcc ggaaaagtg 
      gtcggcaaa tgtttgtac gagagtgct ttaggcggc 
      tcctaccgc caattagtt cgtgccaga ttcacccgt 
      tcctttctg gcgcaagta atcggcctt cgagttcac 
      tacaaatat ttcgagcaa gacgatccc gacctatac 
      ttgtccaaa atcaagtat ttgctcgaa aacgacatc 
      gatcagatc gacaccgaa ctgtatttc gttgaggaa 
      cagtacgat acctcaggc cagttgctt aaaactgtt 
      gaactgata ccgaacggt tcgaaaatt cgcgtgaga 
      aacgccacg aaattgcag tatttggac gctttggct 
      cagtatcgt ctggccacg agtatcaaa gacgagatg 
      gaggcgttt ctcaaaggt ttaaacgag cttattcct 
      gacaatctt ttgagcatt ttcgacgaa aacgaactg 
      gaattactg ctctgcggg acgggtcag tatagtatt 
      gccgatttt aaacaacat catgttatt aatggaaat 
      tcggccgaa tttcgacga attgtaggc tggttttgg 
      gctgctgtt gggaatttt acacaagag gaaatggca 
      aggctactg cagttcact acagggtgt tcgcagttg 
      ccgccaggt ggttttaaa gaattgact ccgaaattt 
      caaattact gcagcccca acttttggg aatcttcca 
      acagcgcat acttgtttt aatcagctt tgtttgccg 
      gattacgac tgttatgag cattttgaa aaatcgctt 
      cttctggcg ataagcgaa gggacggaa ggcttcggc 
      atggtttaa
    mRNA sequence
    • >TC007799 RA
      gcggccggt tgctaaaag tgggcacag ccgtctcaa 
      ccaaactcg aaaaaaagc caaaatgta acgaaaatt 
      tcgtaaaat tgtattatg aagcgttgt gccaaacta 
      ttcgctgtt gtatttttg gtttttgtt agtgttgct 
      agtgtgata agactgtgt ttttgcttt tggtctgga 
      ttatttgaa tcggacaat aaaccggat ggtaaagtc 
      gatgaatgg ctagaacgg aacaatttg tcaccgttc 
      aaggactgt tttgttgac aaaggcata tcccagctg 
      gcccaatgc ggccaattg ggcgatttg ccagaactt 
      cccgcctcc gacgagcaa cgtctccaa aaggcagcc 
      gccttgtta cagcaacgt ctcgtcctc cgccaatgg 
      ctaacaaaa tacaacctc cagcactac tatcctcga 
      ctccttaac ctcgaagtg acaagcttg gaggacgtc 
      tattggtta gaagacacc aaagctaaa tccatcttg 
      cagaaagac ttccccaag tggtcaact gcaagacaa 
      tccttgccg acacgtaag ccgcacttg gaaacactc 
      aaagccgat ctttggtcc gaagttgtc aagtcaagc 
      agccaccaa gacgcctgg acatggggc ggtatgttg 
      gtcgtttct gtgtcagtt gccggtttg gtaacgtta 
      gctgcaatg acccagccg tcattggcc cccgaagct 
      aaacattcc cttctccag tatgtaacc ggaaaatat 
      ctactacca tcaaactgt aaagtgatt ttccattgg 
      tcggaccct caccctgta ggtcatacg gtttgtttc 
      actgtccag ttttatcag cgcaatggc cagccttat 
      ccgatctgt gatacagac aattttgtt gttgatgtt 
      tgcgaaggt tcacgtaaa atggtggtt ataaccgaa 
      cttggatca ccgaccgat ccaaacagt gctaacatt 
      gccaaagtt aagtttatg gtacgacat gcaggccag 
      tatcgaatc acaattatg gtaagcaat cagcatgtg 
      gcaggaagt ccttttatg aggactttc atcgctggg 
      ccggcttat gcccaaagg acggttgtt gtgaggcat 
      tgtagtacg gttgtgtgt acggcaaat gttgcacat 
      ttgttgtac attgaacca cgggatgag tatggaaat 
      gtttgtagc tatccttca gacgatgat ccgacacag 
      gattacaca gtccatatt acacagtta ggaacctat 
      tctaaagaa tcaaacttg gacaatgac gacaataat 
      tttaccacg gctttagat tacgactgg caaagtcaa 
      cgagtgagt cttcaattg aaatttctc tacgaaggt 
      tgttatcac accacaatt aaatataaa ggaatcgag 
      ttacataat ggcgatttt gacattatt gttttaaac 
      agcatcgat tcaactctt gtccacaaa aatgtggct 
      tcgaaaaac cacaatatt tgctacgaa gctaaatta 
      attggtgtc aataatgag cgactgcaa aaagcgaaa 
      aaagtcctg tgttacgtt tcgcccaaa cagctcatc 
      atcaaggaa ctcatcctg aaatttatc cccaaacgc 
      ttgtacacg ttccggttg tgtccttca accaaattc 
      agttttcaa actgaagcc aaaacgatg gaccacccc 
      actggttat ttcacagtg gacgatggt tgccaacct 
      aaaatcgaa ttaatttca aaagaacgt aacgtcata 
      gcggctact tttgcccaa ttccttctc aaaaacatg 
      ggcggttcc gaaactttt aaagataaa caggacttc 
      ttctaccat gaggtacgc aagtaccat cagaaacac 
      taccatgag aagctctcg atgaaagtc aatcgagag 
      aaattgcta gaatcgagc atgaaagca acgaagaat 
      ttttcagtg agcgagtgg tgccgtaac ttcgaaatc 
      acatttcaa ggagaacaa ggcatcgac tggggcggc 
      ttgcgccgc gaatggttc gagctgatt tgctcgcaa 
      cttttcgac gccaaaaac gctctattt accagtttc 
      cacgagggc caacaatcg ctcgtacac ccgaatcca 
      agccgccct tcgcaccta aaactgcgc cactacgaa 
      ttcgccgga aaagtggtc ggcaaatgt ttgtacgag 
      agtgcttta ggcggctcc taccgccaa ttagttcgt 
      gccagattc acccgttcc tttctggcg caagtaatc 
      ggccttcga gttcactac aaatatttc gagcaagac 
      gatcccgac ctatacttg tccaaaatc aagtatttg 
      ctcgaaaac gacatcgat cagatcgac accgaactg 
      tatttcgtt gaggaacag tacgatacc tcaggccag 
      ttgcttaaa actgttgaa ctgataccg aacggttcg 
      aaaattcgc gtgagaaac gccacgaaa ttgcagtat 
      ttggacgct ttggctcag tatcgtctg gccacgagt 
      atcaaagac gagatggag gcgtttctc aaaggttta 
      aacgagctt attcctgac aatcttttg agcattttc 
      gacgaaaac gaactggaa ttactgctc tgcgggacg 
      ggtcagtat agtattgcc gattttaaa caacatcat 
      gttattaat ggaaattcg gccgaattt cgacgaatt 
      gtaggctgg ttttgggct gctgttggg aattttaca 
      caagaggaa atggcaagg ctactgcag ttcactaca 
      gggtgttcg cagttgccg ccaggtggt tttaaagaa 
      ttgactccg aaatttcaa attactgca gccccaact 
      tttgggaat cttccaaca gcgcatact tgttttaat 
      cagctttgt ttgccggat tacgactgt tatgagcat 
      tttgaaaaa tcgcttctt ctggcgata agcgaaggg 
      acggaaggc ttcggcatg gtttaaaca cacagtatt 
      gttttcctg cacagttaa gtataatat tatttgtga 
      tatttattc acggtacaa aaattcttc ttaatcaaa 
      gtttgaacg agcatatag tgtacagat tgtgataaa 
      gtaatgtaa aacagtgtt cattaaatt attaataac 
      ttaggatag gtttattta agcagccgt attgcttat 
      tctagctga tagagcttt taatgagaa cgagttgtg 
      ctaaataac ggacaaatg tttgagttt gtttcagat 
      ctctgtgta cttaatttg catttatgt aattaattt 
      tttaacatt aaaaatttt agcgagcga ttgttataa 
      tacttgtgt tttttcttg ggaccagtg agtttcaaa 
      ctaaagctt acacaaaat tagaaatct ttaatgaga 
      cgacaatag catttaaaa cgcctttta gacatagtt 
      atgtgctgt tagaattaa atttgacta gaatcgtat 
      acgatgtat tgaagaaat cgagcaaaa tataataaa 
      tttgaaaaa tgcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY