Status
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Gene information
General information
TC007770 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 270613..272907
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007770
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07112

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007770 RA
      MSILRGKNS PFCILRRFN NLKNFAHDS TKTSATSAS 
      ATQAPSPGT FTISGKPSI SVPQDTVKV TLQKPDMDV 
      LVDTTTISR QMVAEQISK SIAFDNIPG PISLRIIHR 
      IWGMVPILS TQITGGAIQ YFLAVGSLL SWSGNMALF 
      KRFFDHYGP VVRLHGPLG GDIVMVSRP EHIAAVFKN 
      EGPYPIRSS LDSVEKYRL QHRKLRHTG PILMFGPEW 
      ENFRKSIEQ PLPQLIARQ YDKIDDACN KFIARIANI 
      RNRQEEVPG TFQKEIYKW CLECMCLVT LDKKLGFLD 
      PCGLSSTSD PGVLLDGLM KATKAIQRC EYGLHLWQF 
      LPTPAWKSL VENCDAIDN VLSKYVHRI QVLIKEKKE 
      ASDGVSKEV NCLMENVLL KPGIMVEDA MTILLDMML 
      IGINATAHT IAFMLYHLA KNPRCQVKL YNEIARQPK 
      KLSKDALAA MPYLQACIK ETLRLKPPI PLLGRILND 
      DLSIYNYHI PRGTYLLMV TSLSSWREE YFEDAHKFM 
      PERWLTPID DMQLFASIP FGYGAKACL GKELAEMQI 
      GALITKILR NFKIEYLYG DINSTNKLW AAPNKKLRF 
      RFAERV
    CDS sequence
    • >TC007770 RA
      atgtccatt ttacgcgga aaaaactcg cctttttgc 
      attttgcga cgcttcaat aacttgaag aacttcgcc 
      catgactcg actaaaaca tcggcgact tctgcatca 
      gcgacccaa gcccccagt cctgggacg tttacaatt 
      tctggtaaa ccgagcatt tctgtgccg caagacacc 
      gttaaagtc acgctccag aagcctgat atggacgtc 
      cttgtcgat accacaaca atcagccgc caaatggtt 
      gccgaacaa atatcgaaa tcaatcgct tttgataat 
      attccggga ccaatcagt ttgagaata attcatagg 
      atttggggg atggtcccg attctgagc acgcaaatc 
      actggaggg gctatccag tatttcttg gccgttggt 
      agtttactg agctggagc gggaacatg gcccttttc 
      aaacgtttt tttgaccat tatggccct gttgtgcgc 
      ctccatggc cctctaggg ggcgatatt gtcatggtg 
      agccggccc gaacacatt gctgctgtc ttcaaaaac 
      gaggggcct tacccaatc cgttccagt ctcgattcg 
      gttgaaaaa tatcgcttg caacaccga aaattgcga 
      catactgga ccgattctc atgtttggg cccgaatgg 
      gaaaacttc cgcaagtcg attgaacag ccccttccg 
      caattaatc gcccgtcaa tatgacaaa attgacgac 
      gcttgcaat aaattcatc gcgcgtatt gccaacata 
      cggaaccgc caagaggag gttcctggc acgttccag 
      aaagaaatt tacaagtgg tgtctggaa tgcatgtgt 
      ttggtcacg cttgataaa aaattgggt tttttggat 
      ccttgcggt ttgagttca acgtcggat cctggtgtg 
      ttactcgac ggtttgatg aaggcgact aaagcgata 
      cagcggtgc gaatacggc ctacatttg tggcaattt 
      ttgccaaca ccggcatgg aaatcgctt gtggagaat 
      tgcgatgcg attgataat gttttgagc aagtacgtg 
      caccgtatc caggttttg attaaagag aagaaggaa 
      gccagtgat ggtgttagt aaggaagtg aattgtttg 
      atggagaat gtgctttta aagccggga attatggtc 
      gaagatgcc atgactatt ttgcttgat atgatgctg 
      attggcatt aatgcaaca gcacacacg attgcgttc 
      atgttgtat cacttggca aaaaatccg aggtgtcag 
      gtgaaactc tacaatgag attgcgcgt cagcctaag 
      aaattatcc aaagatgct ttggcggca atgccatat 
      ctccaggcg tgtattaag gaaactttg agacttaaa 
      ccaccgatt cccttgtta gggcgtatt ctcaatgat 
      gatttgtcg atttacaat tatcatatt ccacgcggg 
      acgtattta ttgatggta acaagtctt tcaagttgg 
      cgagaagag tatttcgag gatgctcat aaatttatg 
      cctgaacgt tggctgacg cccattgat gatatgcaa 
      ctatttgcg tctatacct ttcggttat ggagctaaa 
      gcttgcttg ggtaaggaa ctagctgag atgcaaatt 
      ggagctttg ataactaag attttaagg aactttaag 
      attgagtat ctttatggc gatatcaac agtactaac 
      aaactgtgg gctgcgcca aataagaaa ctaagattt 
      agatttgct gaacgagtt taa
    mRNA sequence
    • >TC007770 RA
      gaaaattac tgtaaaatt ttgcaaaat ctagagcgc 
      caagccggc tgtttgaaa aaaaaaaat tcgagacac 
      gcgtccagt tggggttaa agtgttaaa aaccggcat 
      ttggaggta aaatctgtg caatttttt caattagaa 
      caataagat tcaacaatt ttgtcgttt tgtcgcaat 
      aataacgtc ttaaaagac caaatttgg cgttaattc 
      aatcccacc atagtagcc tatgctacg ccattaatt 
      ttttcagca ctacaaccg acgctgttt gaaaaatca 
      aaaatttgg gcaaaatgt ccattttac gcggaaaaa 
      actcgcctt tttgcattt tgcgacgct tcaataact 
      tgaagaact tcgcccatg actcgacta aaacatcgg 
      cgacttctg catcagcga cccaagccc ccagtcctg 
      ggacgttta caatttctg gtaaaccga gcatttctg 
      tgccgcaag acaccgtta aagtcacgc tccagaagc 
      ctgatatgg acgtccttg tcgatacca caacaatca 
      gccgccaaa tggttgccg aacaaatat cgaaatcaa 
      tcgcttttg ataatattc cgggaccaa tcagtttga 
      gaataattc ataggattt gggggatgg tcccgattc 
      tgagcacgc aaatcactg gaggggcta tccagtatt 
      tcttggccg ttggtagtt tactgagct ggagcggga 
      acatggccc ttttcaaac gtttttttg accattatg 
      gccctgttg tgcgcctcc atggccctc tagggggcg 
      atattgtca tggtgagcc ggcccgaac acattgctg 
      ctgtcttca aaaacgagg ggccttacc caatccgtt 
      ccagtctcg attcggttg aaaaatatc gcttgcaac 
      accgaaaat tgcgacata ctggaccga ttctcatgt 
      ttgggcccg aatgggaaa acttccgca agtcgattg 
      aacagcccc ttccgcaat taatcgccc gtcaatatg 
      acaaaattg acgacgctt gcaataaat tcatcgcgc 
      gtattgcca acatacgga accgccaag aggaggttc 
      ctggcacgt tccagaaag aaatttaca agtggtgtc 
      tggaatgca tgtgtttgg tcacgcttg ataaaaaat 
      tgggttttt tggatcctt gcggtttga gttcaacgt 
      cggatcctg gtgtgttac tcgacggtt tgatgaagg 
      cgactaaag cgatacagc ggtgcgaat acggcctac 
      atttgtggc aatttttgc caacaccgg catggaaat 
      cgcttgtgg agaattgcg atgcgattg ataatgttt 
      tgagcaagt acgtgcacc gtatccagg ttttgatta 
      aagagaaga aggaagcca gtgatggtg ttagtaagg 
      aagtgaatt gtttgatgg agaatgtgc ttttaaagc 
      cgggaatta tggtcgaag atgccatga ctattttgc 
      ttgatatga tgctgattg gcattaatg caacagcac 
      acacgattg cgttcatgt tgtatcact tggcaaaaa 
      atccgaggt gtcaggtga aactctaca atgagattg 
      cgcgtcagc ctaagaaat tatccaaag atgctttgg 
      cggcaatgc catatctcc aggcgtgta ttaaggaaa 
      ctttgagac ttaaaccac cgattccct tgttagggc 
      gtattctca atgatgatt tgtcgattt acaattatc 
      atattccac gcgggacgt atttattga tggtaacaa 
      gtctttcaa gttggcgag aagagtatt tcgaggatg 
      ctcataaat ttatgcctg aacgttggc tgacgccca 
      ttgatgata tgcaactat ttgcgtcta tacctttcg 
      gttatggag ctaaagctt gcttgggta aggaactag 
      ctgagatgc aaattggag ctttgataa ctaagattt 
      taaggaact ttaagattg agtatcttt atggcgata 
      tcaacagta ctaacaaac tgtgggctg cgccaaata 
      agaaactaa gatttagat ttgctgaac gagtttaat 
      tttttgtgt ctgtgttta gttgttgtt ggagttaat 
      taaaataaa t
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