Status
Please wait ...
Gene information
General information
TC007762 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 319296..321677
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007762
      iBeetle screen iB_07107

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007762 RA
        MKSLVFLLP LVLSTASNP LRQPKEIAQ RRQLLGYVN 
        NIVHENPNY PDQAAQSSV KVNFNTDQR QIYNYQQQQ 
        QQQPEQSQL FNVGYSVRF TNNNNNRPT IKQTRSNNL 
        ENAEIITGT RKQNEEVQS PQDFISSKK HKISGIKPK 
        PQVVSSFPN PKILLTQAN LSPATQKYY KALEQAKPQ 
        IANVQDLQQ KYSWRNLSP GVEIIKSTE VPTVKYTRT 
        ETGFDHVKA LGRSQDFDQ SNAVENQQN VLYFARPQQ 
        QQQVPLVAK YQIDTSLLR EQVPIQVDA RFVNEEMRK 
        LGLYANPNV NQPVQEVNY LQQAYRTIP DSSQLRPVQ 
        IVYQQQEPE QNRFQYKIY GKPQPINRQ AEEPVLRQP 
        RFLKDLYTN MRPPPFDST YQRIVRVYK
      CDS sequence
      • >TC007762 RA
        atgaaatca ctcgtattc ctcctccct ttggtcctc 
        tcaacagcc tcaaaccct ctccgccaa cccaaagaa 
        atcgcccaa agacgtcag ctcctcggt tacgtcaac 
        aacatcgtc cacgaaaac cccaattat cccgatcaa 
        gcagcacaa agttcagtc aaagtcaac ttcaacact 
        gaccaacga caaatctac aattatcaa caacaacaa 
        caacaacaa cccgaacaa tcccaacta ttcaatgtt 
        ggatacagc gtccgtttt accaacaac aacaacaac 
        cgccccacg attaaacaa accagaagc aacaacttg 
        gaaaatgct gaaattata acaggaact aggaaacaa 
        aacgaagaa gttcaaagt cctcaggat ttcatcagc 
        agcaaaaaa cacaaaatc tcaggaatt aaacccaag 
        ccacaagtc gtttccagc ttccctaac ccgaaaatc 
        ctcctaact caagcgaac ttatcacca gccacccaa 
        aaatactac aaggcttta gaacaagcc aaaccacaa 
        atcgcaaac gtgcaagat ttacaacaa aagtactcc 
        tggaggaac ctaagcccg ggcgtcgaa atcataaaa 
        agcaccgaa gtcccaact gttaaatac acaagaact 
        gagacaggt tttgaccac gttaaggcc cttggcagg 
        agccaagac tttgaccaa tcaaacgcc gtcgaaaac 
        caacaaaat gtattatat ttcgcacgt ccccaacaa 
        caacagcaa gtcccactt gtggccaaa taccaaata 
        gacacgtcc ttattgcga gagcaagtc ccaattcag 
        gtcgatgcg cgttttgtc aacgaagaa atgcgaaaa 
        ttgggactt tatgcaaat cctaatgtt aatcagccg 
        gttcaagag gttaattat ctccaacaa gcttataga 
        acaattccg gacagttca caattgaga ccagtccaa 
        atcgtctac caacaacaa gaaccggag caaaaccgt 
        tttcagtat aaaatttac ggaaaacct caaccgatt 
        aatcgacaa gccgaagag ccagtttta cgtcaaccg 
        agatttttg aaagatttg tacacaaat atgagaccg 
        ccacctttt gatagcacc tatcaacga attgtacgc 
        gtgtacaaa taa
      mRNA sequence
      • >TC007762 RA
        tgtggtgct ttcaacaac ggcaagttt tacctcaaa 
        aactgaact gaacaaatg gtggcagtg tcgaagctg 
        acaaaatga aatcactcg tattcctcc tccctttgg 
        tcctctcaa cagcctcaa accctctcc gccaaccca 
        aagaaatcg cccaaagac gtcagctcc tcggttacg 
        tcaacaaca tcgtccacg aaaacccca attatcccg 
        atcaagcag cacaaagtt cagtcaaag tcaacttca 
        acactgacc aacgacaaa tctacaatt atcaacaac 
        aacaacaac aacaacccg aacaatccc aactattca 
        atgttggat acagcgtcc gttttacca acaacaaca 
        acaaccgcc ccacgatta aacaaacca gaagcaaca 
        acttggaaa atgctgaaa ttataacag gaactagga 
        aacaaaacg aagaagttc aaagtcctc aggatttca 
        tcagcagca aaaaacaca aaatctcag gaattaaac 
        ccaagccac aagtcgttt ccagcttcc ctaacccga 
        aaatcctcc taactcaag cgaacttat caccagcca 
        cccaaaaat actacaagg ctttagaac aagccaaac 
        cacaaatcg caaacgtgc aagatttac aacaaaagt 
        actcctgga ggaacctaa gcccgggcg tcgaaatca 
        taaaaagca ccgaagtcc caactgtta aatacacaa 
        gaactgaga caggttttg accacgtta aggcccttg 
        gcaggagcc aagactttg accaatcaa acgccgtcg 
        aaaaccaac aaaatgtat tatatttcg cacgtcccc 
        aacaacaac agcaagtcc cacttgtgg ccaaatacc 
        aaatagaca cgtccttat tgcgagagc aagtcccaa 
        ttcaggtcg atgcgcgtt ttgtcaacg aagaaatgc 
        gaaaattgg gactttatg caaatccta atgttaatc 
        agccggttc aagaggtta attatctcc aacaagctt 
        atagaacaa ttccggaca gttcacaat tgagaccag 
        tccaaatcg tctaccaac aacaagaac cggagcaaa 
        accgttttc agtataaaa tttacggaa aacctcaac 
        cgattaatc gacaagccg aagagccag ttttacgtc 
        aaccgagat ttttgaaag atttgtaca caaatatga 
        gaccgccac cttttgata gcacctatc aacgaattg 
        tacgcgtgt acaaataaa aaatacttg ttattggta 
        cgattatat tatagagct tatttcttg tactggtta 
        gttgtttaa gtttttatt tattacttt gttatacag 
        aatttttca ataaagtcg agtgtttca g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY