Status
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Gene information
General information
TC031071
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004006,TC004009,TC004010
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 30291365..30377458
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031071
    iBeetle screen iB_03670

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    iBeetle screen iB_07100

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03670

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031071 RA
      MYSLQQFDS CYCDQYNPL VAQRLFNPY FPHLWFPLP 
      VKLQQPRPE KPPYSYIAL IAMAISSAP KERLTLSGI 
      YRFIMDNFP YYRENKQGW QNSIRHNLS LNDCFVKVP 
      RDKVSPRGP EQAGGKGSY WMLDPKAAN MFEKGNYRR 
      RKTRRQRIA DCTANLYQE QALSLVTRP PEKATTQSD 
      QESQRKSKN STLFTIENL IKSNSSKHD YNNYYCYIS 
      LQNGANTAH WSVWWVVVM VVVEMWRPM VVVEVRPTM 
      PMVTPMMPS MVPTKNEKN EKNEKNEKN EKNEKNEKN 
      EKNEKNEKN EKNEKNEKN EKNEKNEKN EKNEKNEKN 
      EKNEKNEKN EKNEKNEKY EKNEKNEKN EKNEKNEKN 
      ERNEKFKKP YINQISFAF GSLVLRQWC VMVAKIFWV 
      VAVLGAVTA EPPSTNYGT PLANSINNF AGYDGNAGN 
      SYDHGHGGH GDHGDHGEP KAYEFGYQV KDEYSGNNY 
      NRKEASDGN QVRGEYRVQ LPDGRTQIV TYYADWQTG 
      FHADVRYEG EAHYPEQYN TYQGGYQGQ NTGGNNYNS 
      GYNSGNNYD SYSAGYGNK GRPSYEYGA P
    CDS sequence
    • >TC031071 RA
      atgtactcc ttacaacag ttcgattct tgttactgt 
      gaccagtac aacccttta gtggcccag cgtctcttc 
      aacccctat ttcccccat ttgtggttc ccgttgccg 
      gtcaagtta caacaaccc cgtccggaa aaaccgccc 
      tattcttac atcgccctc attgccatg gcgatcagc 
      tcagccccc aaggagcgg ctcacgctg agcgggatc 
      tacaggttc atcatggat aattttcca tactacaga 
      gagaacaag caaggatgg cagaactcg attcggcat 
      aatttgagt ttgaatgac tgttttgtt aaagtgccg 
      agggacaaa gtgtcgcca agggggccc gagcaggcg 
      ggggggaag gggagttat tggatgttg gacccgaaa 
      gctgcaaat atgttcgaa aaggggaac tacagaagg 
      aggaagacg aggaggcag aggattgcc gattgtacg 
      gcgaatttg taccaggag caggccctc agtttggtg 
      actaggccg ccggagaag gcgacgacg caatcggac 
      caagaatcg cagagaaaa agcaagaat tccactcta 
      tttaccatc gagaattta attaaaagc aacagctcg 
      aaacacgat tacaataat tactattgt tacatcagt 
      ttacaaaat ggtgcaaat acagcacac tggtcggtg 
      tggtgggtg gtggtgatg gtggtggtc gaaatgtgg 
      aggcccatg gtggtggtc gaagtgagg cctaccatg 
      cccatggtg acccccatg atgccctcc atggtgccc 
      accaagaat gagaagaat gagaagaat gagaagaat 
      gagaagaat gagaagaat gagaagaat gagaagaat 
      gagaagaat gagaagaat gagaagaat gagaagaat 
      gagaagaat gagaagaat gagaagaat gagaagaat 
      gagaagaat gagaagaat gagaagaat gagaagaat 
      gagaagaat gagaagaat gagaagaat gagaagaat 
      gaaaagaat gaaaagtat gagaagaat gagaagaat 
      gagaagaat gagaagaat gagaagaat gagaagaat 
      gagaggaat gagaagttt aagaaacca tatataaac 
      caaattagt tttgcgttc ggcagtctt gtgttgagg 
      cagtggtgc gtgatggtg gctaagatt ttttgggtc 
      gttgcggtt ttgggggcg gtaacggcc gaacccccg 
      agcaccaac tatgggacg ccactggct aacagcatc 
      aataatttt gcgggatac gatgggaat gccgggaat 
      tcgtacgac catgggcat gggggtcat ggggaccat 
      ggggaccat ggtgagccc aaagcgtac gaattcgga 
      taccaggtc aaagacgag tactccgga aacaactac 
      aaccggaaa gaagccagc gatggtaat caggtgaga 
      ggggagtac cgggttcaa ctaccagat ggtcggacc 
      cagattgtt acctattat gcggactgg cagacggga 
      ttccacgcc gatgtcagg tacgaaggc gaggcgcat 
      tacccggaa cagtacaac acctaccaa ggcgggtat 
      caggggcag aataccgga ggaaataac tacaattcc 
      gggtacaat tccgggaac aattacgac tcgtactcg 
      gcgggttat ggcaacaaa gggaggccc agttatgag 
      tatggagca ccataa
    mRNA sequence
    • >TC031071 RA
      cgacttaag ccaagatgt attagccga aatttagtc 
      caatgtcct tccactcac tcagtcaat aaacgcgat 
      gtactcctt acaacagtt cgattcttg ttactgtga 
      ccagtacaa ccctttagt ggcccagcg tctcttcaa 
      cccctattt cccccattt gtggttccc gttgccggt 
      caagttaca acaaccccg tccggaaaa accgcccta 
      ttcttacat cgccctcat tgccatggc gatcagctc 
      agcccccaa ggagcggct cacgctgag cgggatcta 
      caggttcat catggataa ttttccata ctacagaga 
      gaacaagca aggatggca gaactcgat tcggcataa 
      tttgagttt gaatgactg ttttgttaa agtgccgag 
      ggacaaagt gtcgccaag ggggcccga gcaggcggg 
      ggggaaggg gagttattg gatgttgga cccgaaagc 
      tgcaaatat gttcgaaaa ggggaacta cagaaggag 
      gaagacgag gaggcagag gattgccga ttgtacggc 
      gaatttgta ccaggagca ggccctcag tttggtgac 
      taggccgcc ggagaaggc gacgacgca atcggacca 
      agaatcgca gagaaaaag caagaattc cactctatt 
      taccatcga gaatttaat taaaagcaa cagctcgaa 
      acacgatta caataatta ctattgtta catcagttt 
      acaaaatgg tgcaaatac agcacactg gtcggtgtg 
      gtgggtggt ggtgatggt ggtggtcga aatgtggag 
      gcccatggt ggtggtcga agtgaggcc taccatgcc 
      catggtgac ccccatgat gccctccat ggtgcccac 
      caagaatga gaagaatga gaagaatga gaagaatga 
      gaagaatga gaagaatga gaagaatga gaagaatga 
      gaagaatga gaagaatga gaagaatga gaagaatga 
      gaagaatga gaagaatga gaagaatga gaagaatga 
      gaagaatga gaagaatga gaagaatga gaagaatga 
      gaagaatga gaagaatga gaagaatga gaagaatga 
      aaagaatga aaagtatga gaagaatga gaagaatga 
      gaagaatga gaagaatga gaagaatga gaagaatga 
      gaggaatga gaagtttaa gaaaccata tataaacca 
      aattagttt tgcgttcgg cagtcttgt gttgaggca 
      gtggtgcgt gatggtggc taagatttt ttgggtcgt 
      tgcggtttt gggggcggt aacggccga acccccgag 
      caccaacta tgggacgcc actggctaa cagcatcaa 
      taattttgc gggatacga tgggaatgc cgggaattc 
      gtacgacca tgggcatgg gggtcatgg ggaccatgg 
      ggaccatgg tgagcccaa agcgtacga attcggata 
      ccaggtcaa agacgagta ctccggaaa caactacaa 
      ccggaaaga agccagcga tggtaatca ggtgagagg 
      ggagtaccg ggttcaact accagatgg tcggaccca 
      gattgttac ctattatgc ggactggca gacgggatt 
      ccacgccga tgtcaggta cgaaggcga ggcgcatta 
      cccggaaca gtacaacac ctaccaagg cgggtatca 
      ggggcagaa taccggagg aaataacta caattccgg 
      gtacaattc cgggaacaa ttacgactc gtactcggc 
      gggttatgg caacaaagg gaggcccag ttatgagta 
      tggagcacc ataattgag caaattatt aacacaatt 
      ttattattt attttttgt atataagta attgtatac 
      tttttgtac atttttgta tttaaataa ataaatcaa 
      actaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY