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Gene information
General information
TC011435 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5036657..5038956
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011435
    iBeetle screen iB_07093

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011435 RA
      MGRLKTEQK QKSWSEINK IRKEEKRKW KEQNLAKKL 
      EKQKVPETP QVSPNKKAP PTVSIAVPG SILETAQSP 
      ELRAYLSGQ IARAACIFQ VDEIVVFDD YGDESTAKK 
      AELEDNYGL KTMRESCVQ LGRILQYME CPQYLRKHF 
      FPIHNHLKF CGILNPLNA PHHLTKDEE FEFREGVVL 
      NKPVKPGRG SIVNVGLLK EVHVDKLLT PGIRCTVKL 
      SAQQESSKK LKGTVVAPS TPKSETGVY WGYTIRLAN 
      SLSKVFSQS PYKDGYDLT IGTSDKGTS VDEFKCPTF 
      KHVLVVFGG VQGLELALE SDSVLNVDD PKFLFDHYL 
      NTLPNQGSK TIRTEEAIL VTLAALRPK FNDSVEVNE
    CDS sequence
    • >TC011435 RA
      atggggcgc ctaaaaacc gagcaaaaa cagaaaagt 
      tggagcgag attaacaaa atccgcaag gaggagaag 
      cgcaaatgg aaggagcaa aacctggcg aaaaaatta 
      gagaagcag aaagtgcca gaaaccccc caagtgtcc 
      cccaacaaa aaggcgccc cccactgtc tcaatcgcc 
      gtccctggc tcaatcctc gaaacggcc cagtccccc 
      gaactccgc gcctactta tcgggccaa atcgcccgt 
      gcagcttgt attttccaa gttgacgaa attgtagtt 
      tttgacgat tatggtgac gaatcaacg gctaaaaaa 
      gccgaactg gaggacaac tacgggctg aaaacaatg 
      cgcgagagt tgtgtgcaa ctggggcgc attttgcag 
      tacatggag tgtcctcag tacttgcgc aagcatttt 
      tttcccatt cataatcat ttgaaattt tgcgggatt 
      ttgaatccg ctaaatgcg ccccatcat ctgacgaag 
      gacgaggag tttgagttt agggaaggg gtggttttg 
      aataaaccg gtgaagccg ggacggggc tcaattgtg 
      aatgtgggg ctgttgaag gaagtacat gttgataag 
      ttgctaaca cctgggata aggtgtact gttaagttg 
      tcagcacaa caggaatcg agcaaaaag ttgaaaggg 
      acggttgtg gccccctcg accccgaaa agcgaaacg 
      ggggtttac tgggggtac actatcagg ttggctaat 
      tcgttgtcg aaagttttt tcgcaaagt ccctacaag 
      gatggttat gatctgact ataggcact tcagataaa 
      ggaacctcc gttgatgaa ttcaaatgc cccactttt 
      aagcacgtt ttagttgtt tttggtggg gtccaagga 
      ctggaactg gcgttagag agtgacagt gttttaaat 
      gtggatgat ccaaaattt ctttttgat cattattta 
      aacactttg cccaaccaa ggatccaaa acaataagg 
      accgaagaa gccatttta gtgaccttg gccgccttg 
      agacccaaa tttaatgat agtgttgaa gtgaatgaa 
      taa
    mRNA sequence
    • >TC011435 RA
      aagaagtag aaaaaaaag atgcaacaa taaattttt 
      tattattaa agttgcgag ctatctatt gacagtcaa 
      tgacagtga cagtgacac ttgagtgag gttatctca 
      caccatggg gcgcctaaa aaccgagca aaaacagaa 
      aagttggag cgagattaa caaaatccg caaggagga 
      gaagcgcaa atggaagga gcaaaacct ggcgaaaaa 
      attagagaa gcagaaagt gccagaaac cccccaagt 
      gtcccccaa caaaaaggc gccccccac tgtctcaat 
      cgccgtccc tggctcaat cctcgaaac ggcccagtc 
      ccccgaact ccgcgccta cttatcggg ccaaatcgc 
      ccgtgcagc ttgtatttt ccaagttga cgaaattgt 
      agtttttga cgattatgg tgacgaatc aacggctaa 
      aaaagccga actggagga caactacgg gctgaaaac 
      aatgcgcga gagttgtgt gcaactggg gcgcatttt 
      gcagtacat ggagtgtcc tcagtactt gcgcaagca 
      tttttttcc cattcataa tcatttgaa attttgcgg 
      gattttgaa tccgctaaa tgcgcccca tcatctgac 
      gaaggacga ggagtttga gtttaggga aggggtggt 
      tttgaataa accggtgaa gccgggacg gggctcaat 
      tgtgaatgt ggggctgtt gaaggaagt acatgttga 
      taagttgct aacacctgg gataaggtg tactgttaa 
      gttgtcagc acaacagga atcgagcaa aaagttgaa 
      agggacggt tgtggcccc ctcgacccc gaaaagcga 
      aacgggggt ttactgggg gtacactat caggttggc 
      taattcgtt gtcgaaagt tttttcgca aagtcccta 
      caaggatgg ttatgatct gactatagg cacttcaga 
      taaaggaac ctccgttga tgaattcaa atgccccac 
      ttttaagca cgttttagt tgtttttgg tggggtcca 
      aggactgga actggcgtt agagagtga cagtgtttt 
      aaatgtgga tgatccaaa atttctttt tgatcatta 
      tttaaacac tttgcccaa ccaaggatc caaaacaat 
      aaggaccga agaagccat tttagtgac cttggccgc 
      cttgagacc caaatttaa tgatagtgt tgaagtgaa 
      tgaataaat aagttttgt aatgtttta caataaaaa 
      tattttaag taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY