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Gene information
General information
TC003915 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26737806..26747178
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003915
    iBeetle screen iB_07083

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003915 RA
      MSGSGKPLL GLWLYRSGE DWSYKEDCP LQTAATGAN 
      QALNEEPKP KVEKISVIF TLKNQVGGL VRALQAFQD 
      LGINVNHIE SRPSQLADN QVDFLVDID TDQRKLDQL 
      GRLLKREVQ TMVIGTYGN GEENEFPPP TPLSATTSF 
      DFDDMPWFP KKISELDQA QNVLMYGAE LDADHPGFK 
      DPVYRKRRE QFFRIAMNY KHGQPIPKV QYTPEEIKT 
      WGTVFRELH KLYQKHACR EYLENWPQL EKYCGYRED 
      NIPQLEDVN VFLKRKTGF QLRPVAGYL SPRDFLSGL 
      AFRVFHCTQ YIRHSSDPF YTPEPDCCH ELLGHMPLL 
      ANPSFAQFS QELGLASLG ASEEDVNKL ATLYFFTVE 
      FGLCKQEGE MRVYGAGLL SSVAELRHA INTPEKIKR 
      FDPDETVKQ ECIITSFQL GYWYTDSFE EAKEKMRTF 
      AEQIQRPFG LRYNPYTQS VEVLSNEQK ITAVVSELR 
      GDLCIVNSA LKKISARHS NLDVDKIAN LLQTGLATS 
      NGEDNQK
    CDS sequence
    • >TC003915 RA
      atgagtggc tccggaaag cctttgctt ggtctttgg 
      ctttaccga agcggcgaa gattggagt tacaaagaa 
      gattgtcct cttcaaact gcagcgacg ggagctaat 
      caggcgcta aacgaggaa ccaaaaccg aaagtcgag 
      aaaatctct gtaatcttc accctaaaa aaccaagtc 
      ggaggtctg gtccgtgcc cttcaggcc ttccaggac 
      ctcggcatc aacgtaaat cacatcgaa tctcgtcca 
      tcgcaactt gccgataat caggtcgac ttcttggtg 
      gatattgac actgatcag cgtaaatta gatcagtta 
      gggcgtcta ctcaaacga gaagtgcaa actatggtc 
      ataggaact tatggaaac ggtgaagag aacgaattc 
      cctcctcct actccatta tcggcaact acgagcttc 
      gattttgat gacatgcct tggtttccg aagaaaatc 
      tcagagctt gatcaagca caaaatgtg ctaatgtat 
      ggagctgag cttgatgct gatcatcca gggtttaaa 
      gatcccgtc tacaggaaa aggcgagaa cagttcttt 
      agaattgca atgaattat aaacatggg cagccaata 
      ccgaaggtt cagtatact ccagaagaa ataaaaacg 
      tggggaacc gtcttccgt gaactccac aaactttac 
      cagaagcac gcctgtcga gagtacttg gaaaattgg 
      cctcagttg gaaaaatac tgcggctat cgagaggac 
      aatatccca caactggag gacgtaaac gtatttcta 
      aagcgaaaa acgggcttt caactgcgc ccagttgct 
      ggctacttg agtccaaga gattttctc tctggctta 
      gctttccgt gtctttcac tgcacccaa tacattcga 
      cactcttca gacccgttt tacactcca gaaccagac 
      tgttgccac gaattattg ggccacatg cctttgctt 
      gcaaaccca agctttgcc cagttttcc caagagttg 
      ggtttggcc tcacttgga gcttcagaa gaggatgtt 
      aacaaattg gctacactc tattttttt acggttgag 
      ttcggtttg tgcaaacaa gaaggcgaa atgcgcgtt 
      tatggtgca ggtctttta agttcagtt gcagaactc 
      cggcacgct attaacact ccggaaaaa ataaaacgt 
      ttcgatccg gacgaaacc gttaaacag gaatgcata 
      ataacgtcg tttcagctt ggttattgg tacactgat 
      tcgttcgaa gaagctaag gagaaaatg agaacattt 
      gccgaacaa attcagcgg ccgttcggt ctgcgttac 
      aatccgtac actcagagt gtggaagtt ttgagcaat 
      gagcagaaa attacagct gtggtttcg gaattacga 
      ggcgatttg tgtattgta aacagtgct ttgaagaaa 
      attagcgct cggcactct aacctggat gttgataaa 
      attgctaat ctcctgcaa actggactt gctacctca 
      aatggcgaa gacaatcaa aaataa
    mRNA sequence
    • >TC003915 RA
      ttgagttta taaaagctg atgcgggaa tcttaatcg 
      ttacaaacc tgttggtct ttacaagaa tatagtaag 
      aatgagtgg ctccggaaa gcctttgct tggtctttg 
      gctttaccg aagcggcga agattggag ttacaaaga 
      agattgtcc tcttcaaac tgcagcgac gggagctaa 
      tcaggcgct aaacgagga accaaaacc gaaagtcga 
      gaaaatctc tgtaatctt caccctaaa aaaccaagt 
      cggaggtct ggtccgtgc ccttcaggc cttccagga 
      cctcggcat caacgtaaa tcacatcga atctcgtcc 
      atcgcaact tgccgataa tcaggtcga cttcttggt 
      ggatattga cactgatca gcgtaaatt agatcagtt 
      agggcgtct actcaaacg agaagtgca aactatggt 
      cataggaac ttatggaaa cggtgaaga gaacgaatt 
      ccctcctcc tactccatt atcggcaac tacgagctt 
      cgattttga tgacatgcc ttggtttcc gaagaaaat 
      ctcagagct tgatcaagc acaaaatgt gctaatgta 
      tggagctga gcttgatgc tgatcatcc agggtttaa 
      agatcccgt ctacaggaa aaggcgaga acagttctt 
      tagaattgc aatgaatta taaacatgg gcagccaat 
      accgaaggt tcagtatac tccagaaga aataaaaac 
      gtggggaac cgtcttccg tgaactcca caaacttta 
      ccagaagca cgcctgtcg agagtactt ggaaaattg 
      gcctcagtt ggaaaaata ctgcggcta tcgagagga 
      caatatccc acaactgga ggacgtaaa cgtatttct 
      aaagcgaaa aacgggctt tcaactgcg cccagttgc 
      tggctactt gagtccaag agattttct ctctggctt 
      agctttccg tgtctttca ctgcaccca atacattcg 
      acactcttc agacccgtt ttacactcc agaaccaga 
      ctgttgcca cgaattatt gggccacat gcctttgct 
      tgcaaaccc aagctttgc ccagttttc ccaagagtt 
      gggtttggc ctcacttgg agcttcaga agaggatgt 
      taacaaatt ggctacact ctatttttt tacggttga 
      gttcggttt gtgcaaaca agaaggcga aatgcgcgt 
      ttatggtgc aggtctttt aagttcagt tgcagaact 
      ccggcacgc tattaacac tccggaaaa aataaaacg 
      tttcgatcc ggacgaaac cgttaaaca ggaatgcat 
      aataacgtc gtttcagct tggttattg gtacactga 
      ttcgttcga agaagctaa ggagaaaat gagaacatt 
      tgccgaaca aattcagcg gccgttcgg tctgcgtta 
      caatccgta cactcagag tgtggaagt tttgagcaa 
      tgagcagaa aattacagc tgtggtttc ggaattacg 
      aggcgattt gtgtattgt aaacagtgc tttgaagaa 
      aattagcgc tcggcactc taacctgga tgttgataa 
      aattgctaa tctcctgca aactggact tgctacctc 
      aaatggcga agacaatca aaaataatt tagaattaa 
      gcaaagtta tttgtggac cagattata tatgagtag 
      ttgttggca gttaataaa tattcatga tcaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY