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Gene information
General information
TC003883 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25859144..25861084
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003883
    iBeetle screen iB_07079

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003883 RA
      MSRSAQRGG LNPEPDSPK ISILFLDIT RFSQPVQFF 
      ICSGAVFVF FILYGYMQE LIFTIDGFQ PFGWYLTLV 
      QFGFYSVFG LIETRIRNI TSRSIPIQT YFLLALLTL 
      GTMGFSNAS LGYLNYPTQ VIFKCCKLI PVLVGSILI 
      QGKRYGPLD FSAAVLMCV GLTLFTLAD SHVQPNFNT 
      KGIFMISMA LLCDAIIGN VQEKSMKNY GAPNSEVVL 
      FSYSIGFVY LLIVMVATG DFTDGLQFF STNPKKLYG 
      YALIFSLTG YLGIQIVLT LVRTCGAFA AVTVTTCRK 
      AVTIIISFV FFSKPFTFQ YLWSGLLVV LGIYLNLFS 
      KRHPMSMQD LEVFIERCI RTIKLKLYN KRQGPRHYM 
      ANV
    CDS sequence
    • >TC003883 RA
      atgtcacgc agcgctcag cgtgggggg ctcaacccc 
      gagccagac agccccaaa atcagtatc cttttcctc 
      gatatcact cgtttttcc caacctgtc caatttttc 
      atttgcagt ggggctgtt ttcgtgttt ttcatcctt 
      tacgggtac atgcaagag ctgatcttc accatcgac 
      ggcttccag cccttcggg tggtacctc acgttggtc 
      cagttcggg ttttacagc gtctttggc ctgattgag 
      acacgaata aggaacata acctctcga tcaattccg 
      atccaaacg tacttttta ctcgcactg ttgaccctt 
      ggcaccatg ggtttctct aatgcctcg ctcggttac 
      ttgaattat ccgactcaa gtgattttc aagtgttgc 
      aagttaatt ccggtcctt gtcgggagt attctaatt 
      cagggcaaa cggtacggg cctttggac tttagtgca 
      gctgttttg atgtgtgtg ggactaaca ttattcact 
      ctggccgat tcacatgta caaccaaat tttaacact 
      aaaggcatc ttcatgatc tcgatggcc ttgctgtgc 
      gacgcaatt ataggaaat gtgcaggaa aagtcaatg 
      aaaaattat ggggctccg aattccgaa gtcgtcctg 
      ttttcgtat tcaattggg tttgtgtac ctccttatt 
      gtgatggtg gccactggc gacttcacc gatggcttg 
      caatttttt tctactaat ccaaaaaaa ctgtacggt 
      tacgcttta attttttca ctaactggg tatttgggg 
      attcagatc gttctcact ctagtcagg acttgtggg 
      gcttttgcg gcagtcact gtgacaacg tgccgcaaa 
      gccgtgact attatcata tcatttgtt tttttcagc 
      aaacctttt acgtttcag tatttatgg tccggttta 
      ttggtagtt ttaggtatt tatttaaat ttgtttagt 
      aaaaggcat ccaatgtcg atgcaagac ttggaggtg 
      tttatagaa aggtgcatt cgaacaatc aaattaaaa 
      ctttataac aaacgtcaa ggtcctcgc cactatatg 
      gcgaacgtt tga
    mRNA sequence
    • >TC003883 RA
      cgggaattt aatttgtca atttatttt caaaatgat 
      tggggggac gattggcga gcctggtgg gacgtcaag 
      tgtcagatg atttgaata tgtgtcaac gtgtctcaa 
      atcaagcaa aagtaatga ttttttccc gtaattgaa 
      aatgtcacg cagcgctca gcgtggggg gctcaaccc 
      cgagccaga cagccccaa aatcagtat ccttttcct 
      cgatatcac tcgtttttc ccaacctgt ccaattttt 
      catttgcag tggggctgt tttcgtgtt tttcatcct 
      ttacgggta catgcaaga gctgatctt caccatcga 
      cggcttcca gcccttcgg gtggtacct cacgttggt 
      ccagttcgg gttttacag cgtctttgg cctgattga 
      gacacgaat aaggaacat aacctctcg atcaattcc 
      gatccaaac gtacttttt actcgcact gttgaccct 
      tggcaccat gggtttctc taatgcctc gctcggtta 
      cttgaatta tccgactca agtgatttt caagtgttg 
      caagttaat tccggtcct tgtcgggag tattctaat 
      tcagggcaa acggtacgg gcctttgga ctttagtgc 
      agctgtttt gatgtgtgt gggactaac attattcac 
      tctggccga ttcacatgt acaaccaaa ttttaacac 
      taaaggcat cttcatgat ctcgatggc cttgctgtg 
      cgacgcaat tataggaaa tgtgcagga aaagtcaat 
      gaaaaatta tggggctcc gaattccga agtcgtcct 
      gttttcgta ttcaattgg gtttgtgta cctccttat 
      tgtgatggt ggccactgg cgacttcac cgatggctt 
      gcaattttt ttctactaa tccaaaaaa actgtacgg 
      ttacgcttt aattttttc actaactgg gtatttggg 
      gattcagat cgttctcac tctagtcag gacttgtgg 
      ggcttttgc ggcagtcac tgtgacaac gtgccgcaa 
      agccgtgac tattatcat atcatttgt ttttttcag 
      caaaccttt tacgtttca gtatttatg gtccggttt 
      attggtagt tttaggtat ttatttaaa tttgtttag 
      taaaaggca tccaatgtc gatgcaaga cttggaggt 
      gtttataga aaggtgcat tcgaacaat caaattaaa 
      actttataa caaacgtca aggtcctcg ccactatat 
      ggcgaacgt ttgatttta gtgatttaa gcaatactt 
      tgccatatt ttatatttt gttttatcg taatagatg 
      tgttcattt tttattgct ttaagaccg gacccgtcc 
      ttttcactc ccggaagat ggcgctcaa atcaaaata 
      cacaacgct caaagacga acaccaacg caaaactta 
      attatttaa attaacata atatatgtg gatttttca 
      gatttttta ttccacaaa atatgttca aattttgag 
      gtttgtaga ataaaagat cgtattttt cacttttta 
      attcaattg aaatgaatt cactgcata agaaaaaaa 
      tggggacct gtccaacga gttgttcaa atgaaaaaa 
      agaaaaaca aattgaatt tctaagata aataatgag 
      cccaacaat taattgaac aggaagttt aaatatatt 
      tttgtaact gagtttcct ttaagaaaa acatcttat 
      gaaaaaaat acaataaac atagatagg tcca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY