Status
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Gene information
General information
TC032604
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002121
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20006975..20010935
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032604
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07071

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032604 RA
      MTTALQQQA FALDARYNA HRAPESPNF RTLYIRRRS 
      QLLRETQRD PERWKQYLR LRSMLLQQR YGSSAVESS 
      SSNLSRSSS MSTDQHSTV VTPRGHLVP TKQAEASRA 
      LVGGTSIAE NYAFVGVHH IFDQHSEAV TMVKFANND 
      KSTICCASL DFTLSICDV TSEPPTVSA ILRGHSKAV 
      TGFDWSANN DLIVSSSLD GTCRVWKVS DFSCLRIVQ 
      DSNNSQFLC CMFQPINNN LFVTGNSRG ELKIANVST 
      GRFMKNTCK IGGNVLCLT SDNSGKIFW VGNDKGEIV 
      SLFCELNGA LCKTKKILL SPNCSITSL SYRAWISRE 
      ARDPLLLVN ATNNSFCLF SVLDGEGTL QLKKSFQNR 
      QQKHIVRST FCPIMSFRQ GACVVTGSE DGSVYFVDV 
      EKVGNRAVV NTLQGHASA VLGISFNYD ESLLATSDL 
      QGLVILWKK GNLL
    CDS sequence
    • >TC032604 RA
      atgacaaca gccctccaa cagcaagcg ttcgctttg 
      gacgcccgg tacaatgcc caccgtgcc cccgagagc 
      cccaatttc cgaacttta tacataaga cggcgtagc 
      caattacta agagaaact cagcgagat ccagaaagg 
      tggaaacaa tatttacgt cttcgttcc atgctttta 
      caacagcga tatgggtcg tcagctgtt gaatcatct 
      tcatctaat ttatcgaga tctagtagc atgtcaacg 
      gaccaacat tccactgtt gtgacacct aggggacac 
      ttggtgccc actaaacaa gcggaggct tcaagagca 
      ttggttgga gggacttca attgcggaa aattatgct 
      tttgtgggg gtacatcat atatttgac caacacagt 
      gaagctgtt acaatggta aagtttgca aacaatgat 
      aaaagtaca atttgttgc gcttctttg gactttact 
      ttgtccata tgtgatgtt acaagtgaa cctccgaca 
      gtgtctgcg attttgaga gggcatagc aaggctgtg 
      acaggtttt gattggtcg gcaaataat gatttgatt 
      gtgagcagt tctttagac ggaacttgc agagtgtgg 
      aaagtgtca gattttagt tgtttgaga atcgttcaa 
      gattcaaac aactcacag tttttgtgc tgcatgttc 
      cagcctatt aataacaac ttgtttgtt actggtaat 
      agtagagga gaactaaaa attgccaat gtctcgact 
      ggtagattt atgaaaaac acttgtaaa attggcgga 
      aacgtgtta tgtttaacc tcagataat agtggaaaa 
      attttttgg gttggaaat gacaaggga gaaattgtg 
      tcattattt tgcgaactg aacggagct ctttgtaaa 
      acaaagaaa attctattg tcaccaaat tgttcaatt 
      actagtttg agttacaga gcttggata agtagagaa 
      gctcgtgat cctttacta cttgtaaat gcaacaaac 
      aattcgttt tgtttgttt agtgtttta gatggtgaa 
      gggacttta caactgaaa aaaagcttc caaaacaga 
      caacaaaaa cacattgtg agatcaacc ttctgccca 
      attatgtca tttcgtcaa ggggcgtgt gttgttact 
      ggaagcgag gatggaagt gtttatttt gtagacgtt 
      gaaaaagtg ggaaatagg gctgttgtt aacactttg 
      caaggacat gctagtgct gtcttgggc ataagtttt 
      aattatgat gagtcattg ttggccaca agtgatctt 
      caaggactt gttatttta tggaaaaaa ggaaatttg 
      ctataa
    mRNA sequence
    • >TC032604 RA
      taacctcac aaaaaatat agtgtcaaa aattgattt 
      ttgaatttt taagcactt aggccatga caacagccc 
      tccaacagc aagcgttcg ctttggacg cccggtaca 
      atgcccacc gtgcccccg agagcccca atttccgaa 
      ctttataca taagacggc gtagccaat tactaagag 
      aaactcagc gagatccag aaaggtgga aacaatatt 
      tacgtcttc gttccatgc ttttacaac agcgatatg 
      ggtcgtcag ctgttgaat catcttcat ctaatttat 
      cgagatcta gtagcatgt caacggacc aacattcca 
      ctgttgtga cacctaggg gacacttgg tgcccacta 
      aacaagcgg aggcttcaa gagcattgg ttggaggga 
      cttcaattg cggaaaatt atgcttttg tgggggtac 
      atcatatat ttgaccaac acagtgaag ctgttacaa 
      tggtaaagt ttgcaaaca atgataaaa gtacaattt 
      gttgcgctt ctttggact ttactttgt ccatatgtg 
      atgttacaa gtgaacctc cgacagtgt ctgcgattt 
      tgagagggc atagcaagg ctgtgacag gttttgatt 
      ggtcggcaa ataatgatt tgattgtga gcagttctt 
      tagacggaa cttgcagag tgtggaaag tgtcagatt 
      ttagttgtt tgagaatcg ttcaagatt caaacaact 
      cacagtttt tgtgctgca tgttccagc ctattaata 
      acaacttgt ttgttactg gtaatagta gaggagaac 
      taaaaattg ccaatgtct cgactggta gatttatga 
      aaaacactt gtaaaattg gcggaaacg tgttatgtt 
      taacctcag ataatagtg gaaaaattt tttgggttg 
      gaaatgaca agggagaaa ttgtgtcat tattttgcg 
      aactgaacg gagctcttt gtaaaacaa agaaaattc 
      tattgtcac caaattgtt caattacta gtttgagtt 
      acagagctt ggataagta gagaagctc gtgatcctt 
      tactacttg taaatgcaa caaacaatt cgttttgtt 
      tgtttagtg ttttagatg gtgaaggga ctttacaac 
      tgaaaaaaa gcttccaaa acagacaac aaaaacaca 
      ttgtgagat caaccttct gcccaatta tgtcatttc 
      gtcaagggg cgtgtgttg ttactggaa gcgaggatg 
      gaagtgttt attttgtag acgttgaaa aagtgggaa 
      atagggctg ttgttaaca ctttgcaag gacatgcta 
      gtgctgtct tgggcataa gttttaatt atgatgagt 
      cattgttgg ccacaagtg atcttcaag gacttgtta 
      ttttatgga aaaaaggaa atttgctat aatataatt 
      ggtattatt tataacttg tgatagaaa cgtattggt 
      tgtgattta tttttgtaa attgagaaa taaaggtgg 
      tggcctcta ttccagttt ctgaataaa atgtttatt 
      aatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY